Understanding protein-mediated cell signaling and differentiation processes requires capturing response heterogeneity for single cells in large populations. We report single-cell western blots on standard microscope s...
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ISBN:
(纸本)9781632666246
Understanding protein-mediated cell signaling and differentiation processes requires capturing response heterogeneity for single cells in large populations. We report single-cell western blots on standard microscope slides that achieve 103-104 molecule detection limits for >6 protein targets per blot in a
Microfluidic tools are an essential capability in understanding cell-to-cell heterogeneity. Similarly, precision microanalytical tools can play an outsize role in measuring molecule-to-molecule heterogeneity, especial...
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Gradients play a key role in mimicking biological conditions in situ and in separating biological species for analysis. Current methods to generate microscale gradients typically require high reagent consumption and e...
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The disassembly of a core-satellite nanostructured substrate is presented as a colorimetric biosensor observable under dark-field illumination. The fabrication method described herein utilizes thiol-mediated adsorptio...
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We present a technique for information-theoretic optimization of computational imaging systems demonstrated in snapshot 3D microscopy. By directly evaluating measurement quality and decoupling optimization from downst...
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We present a deep-learning method based on Wiener filters and U-Nets that performs image reconstruction in systems with spatially-varying aberrations. We train on simulated microscopy measurements and test on experime...
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Single-cell 'omics is advancing rapidly, but there are gaps in linking nucleic acids to proteins in their myriad forms. To surmount this gap, we describe a microfluidic device that reports information on nucleic a...
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Here we present a fully integrated lab-on-a-chip (LOC) system that incorporates cell preconcentration, polymerase chain reaction (PCR), and capillary electrophoretic (CE) microchannel for rapid and highly sensitive pa...
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