We examined the visibility of motion artifacts - judder, motion blur, and edge banding - on a Samsung 240Hz stereoscopic 3D (S3D) OLED display. We determined the relative contributions of the frame rate of the content...
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Purpose: A calibrationless parallel imaging reconstruction method, termed simultaneous autocalibrating and k-space estimation (SAKE), is presented. It is a data-driven, coil-by-coil reconstruction method that does not...
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Understanding protein-mediated cell signaling and differentiation processes requires capturing response heterogeneity for single cells in large populations. We report single-cell western blots on standard microscope s...
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ISBN:
(纸本)9781632666246
Understanding protein-mediated cell signaling and differentiation processes requires capturing response heterogeneity for single cells in large populations. We report single-cell western blots on standard microscope slides that achieve 103-104 molecule detection limits for >6 protein targets per blot in a
We examined the visibility of motion artifacts—judder, motion blur, and edge banding—on a Samsung 240Hz stereoscopic 3D (S3D) OLED display. We determined the relative contributions of the frame rate of the content, ...
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We examined the visibility of motion artifacts—judder, motion blur, and edge banding—on a Samsung 240Hz stereoscopic 3D (S3D) OLED display. We determined the relative contributions of the frame rate of the content, update rate of the panel, duty cycle, and flash number. We compared the visibility of artifacts on the Samsung display with those on a 60Hz S3D LCD. Short duty cycles and low flash numbers reduce the visibility of motion artifacts.
We translate western blotting to a microchip workflow that cuts conventional assay times from 3-18 hours to 10-60 min with up to 5-plex analyte detection and 54-plex channel throughput. An engineered photoactive prote...
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ISBN:
(纸本)9780979806452
We translate western blotting to a microchip workflow that cuts conventional assay times from 3-18 hours to 10-60 min with up to 5-plex analyte detection and 54-plex channel throughput. An engineered photoactive protein capture matrix with tunable porosity (PACTgel) constitutes 1) a spatially addressable denaturing polyacrylamide gel electrophoresis (SDS-PAGE) separation matrix compatible with mask-based visible light photopatterning and 2) a UV light-toggled protein capture matrix with unprecedented capture efficiency (>75%). We report the first 10 minute western blot consisting of protein sizing and immunoaffinity detection in a single microchannel, and validate a rapid confirmatory HIV diagnostic using human serum. Our capability for rapid confirmatory HIV diagnosis at the point of care has the potential to circumvent the western blotting bottleneck in the current diagnostic pipeline.
We introduce a new microfluidic approach for rapid, integrated two-dimensional electrophoresis (2DE), which uses a fundamentally different design strategy for integrating two the distinct assay stages of isoelectric f...
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Objective We aimto determine the utility of quantifying DXA-derived surrogates of CT measures including thigh cross-sectional muscle area(CSA) and muscle attenuation. Methods We used a retrospective case-cohort study ...
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Objective We aimto determine the utility of quantifying DXA-derived surrogates of CT measures including thigh cross-sectional muscle area(CSA) and muscle attenuation. Methods We used a retrospective case-cohort study design using data from the HealthAging and Body Composition(HealthABC) *** were 111 participants that had a hip fracture during the 8.7-year study period.A cohortof 212 non-fracture participants wasrandomly selected
＜正＞Objective:To examine theindependent association ofbody shape（total volume,trunk volume,and trunk to leg volume ratio）tofracture risk. Methods:We performed a cross-sectional study of the NHANES 1999-2004 survey...
＜正＞Objective:To examine theindependent association ofbody shape（total volume,trunk volume,and trunk to leg volume ratio）tofracture risk. Methods:We performed a cross-sectional study of the NHANES 1999-2004 survey data downloaded from the study *** individualshad both a valid dual-energy Xray absorptiometry（DXA） scan and known fracture status. The body shape measureswere derived using DXA whole
We introduce a 'hands free' proteomic tool with the potential to radically transform the status quo of cancer protein bi-omarker validation. Here, we focus on a challenging and broadly relevant target in this ...
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ISBN:
(纸本)9781618395955
We introduce a 'hands free' proteomic tool with the potential to radically transform the status quo of cancer protein bi-omarker validation. Here, we focus on a challenging and broadly relevant target in this analyte class - the prostate cancer marker prostate-specific antigen (PSA). We introduce light-activatable, volume accessible polyacrylamide separation gels (LAVAgels) that capture electrophoretically separated PSA isoforms within a highly multiplexed (16-channel) microfluidic assay format. The 3D LAVAgel matrix offers unprecedented target capture duty, with capture efficiencies greater than 100fold higher than for existing surface chemistries. Our optofluidic separations strategy harnesses tremendous micro/nanoscale transport advantages to yield unmatched speed and efficiency - major barriers to realizing high-throughput proteomics.
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