检索结果-内蒙古大学图书馆

A reversible water-based electrostatic adhesive

Angewandte Chemie 2023年第2期136卷

作者： Dr. Adriana Sierra-Romero Dr. Katarina Novakovic Prof. Mark Geoghegan School of Engineering Newcastle University Newcastle Upon Tyne NE1 7RU UK Contribution: Data curation (lead) Formal analysis (lead) Investigation (lead) Methodology (equal) Validation (lead) Visualization (lead) Writing - original draft (lead) Writing - review & editing (supporting) Contribution: Funding acquisition (equal) Methodology (equal) Project administration (supporting) Supervision (equal) Writing - review & editing (equal) Contribution: Conceptualization (lead) Funding acquisition (equal) Methodology (equal) Project administration (lead) Supervision (equal) Writing - review & editing (equal)

Commercial adhesives typically fall into two categories: structural or pressure sensitive. Structural glues rely on covalent bonds formed during curing and provide high tensile strength whilst pressure-sensitive adhesives use physical bonding to provide weaker adhesion, but with considerable convenience for the user. Here, a new class of adhesive is presented that is also reversible, with a bond strength intermediate between those of pressure-sensitive and structural adhesives. Complementary water-based formulations incorporating oppositely charged polyelectrolytes form electrostatic bonds that may be reversed through immersion in a low or high pH aqueous environment. This electrostatic adhesive has the advantageous property that it exhibits good adhesion to low-energy surfaces such as polypropylene. Furthermore, it is produced by the emulsion copolymerization of commodity materials, styrene and butyl acrylate, which makes it inexpensive and opens the possibility of industrial production. Bio-based materials have been also integrated into the formulations to further increase sustainability. Moreover, unlike other water-based glues, adhesion does not significantly degrade in humid environments. Because such electrostatic adhesives do not require mechanical detachment, they are appropriate for the large-scale recycling of, e.g., bottle labels or food packaging. The adhesive is also suitable for dismantling components in areas as varied as automotive parts and electronics.

关键词： Adhesion Emulsion Polymers Interfaces Polyelectrolytes

来源：评论

学校读者我要写书评

暂无评论

Cover Image, Volume 140, Issue 42

引用

Journal of Applied Polymer Science 2023年第42期140卷

作者： Haoping Peng Jiaxin Li Yufei Gu Changjun Wu Ya Liu Yonggang Zhao Xuping Su Zhiwei Li Contribution: Conceptualization (equal) Funding acquisition (lead) Project administration (lead) Resources (lead) Contribution: Formal analysis (lead) Investigation (lead) Methodology (lead) Validation (lead) Writing - original draft (lead) Writing - review & editing (lead) Contribution: Conceptualization (equal) Contribution: Conceptualization (equal) Funding acquisition (equal) Contribution: Conceptualization (equal) Resources (equal) Supervision (equal) Visualization (equal) Contribution: Supervision (supporting) Contribution: Funding acquisition (lead) Contribution: Conceptualization (lead) Data curation (equal) Methodology (equal) Supervision (equal) Writing - review & editing (lead)

来源：评论

学校读者我要写书评

暂无评论

A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions

引用

Angewandte Chemie 2022年第37期134卷

作者： Sergi Roda Laura Fernandez-Lopez Marius Benedens Dr. Alexander Bollinger Dr. Stephan Thies Dr. Julia Schumacher Dr. Cristina Coscolín Dr. Masoud Kazemi Dr. Gerard Santiago Dr. Christoph G. W. Gertzen Jose L. Gonzalez-Alfonso Prof. Francisco J. Plou Prof. Karl-Erich Jaeger Prof. Sander H. J. Smits Prof. Manuel Ferrer Prof. Víctor Guallar Department of Life Sciences Barcelona Supercomputing Center Carrer de Jordi Girona 31 08034 Barcelona Spain Contribution: Conceptualization (supporting) Data curation (supporting) Formal analysis (supporting) Methodology (supporting) Software (lead) Writing - original draft (supporting) Department of Applied Biocatalysis ICP CSIC Marie Curie 2 28049 Madrid Spain Contribution: Data curation (supporting) Formal analysis (supporting) Investigation (supporting) Methodology (supporting) Center for Structural Studies Heinrich-Heine-University Building 26.44.01.62 Universitaetsstr 1 40228 Duesseldorf Germany Contribution: Data curation (supporting) Formal analysis (supporting) Methodology (supporting) Institute of Molecular Enzyme Technology Heinrich-Heine-Universität Düsseldorf Building 26.44.01.62 Universitaetsstr 1 40228 Duesseldorf Germany Forschungszentrum Jülich Building 15.8 01/303 52428 Wilhelm Johnen Straße Jülich Germany Contribution: Investigation (supporting) Contribution: Methodology (supporting) Contribution: Conceptualization (supporting) Methodology (supporting) Contribution: Data curation (supporting) Formal analysis (supporting) Methodology (supporting) Resources (supporting) Validation (supporting) Writing - review & editing (supporting) Contribution: Funding acquisition (supporting) Investigation (supporting) Methodology (supporting) Resources (supporting) Contribution: Conceptualization (supporting) Funding acquisition (supporting) Investigation (supporting) Resources (supporting) Supervision (supporting) Contribution: Data curation (lead) Formal analysis (lead) Methodology (lead) Resources (lead) Validation (lead) Visualization (lead) Writing - review & editing (lead) Contribution: Conceptualization (lead) Data curation (lead) Formal analysis (lead) Funding acquisition (lead) Investigation (lead) Methodology (lead) Project administration (lead) Resources (lead) Supervision (lead) Validation (lead) Visualization (lead) Writing -

Engineering dual-function single polypeptide catalysts with two abiotic or biotic catalytic entities (or combinations of both) supporting cascade reactions is becoming an important area of enzyme engineering and catalysis. Herein we present the development of a PluriZyme , TR 2 E 2 , with efficient native transaminase ( k cat : 69.49±1.77 min −1 ) and artificial esterase ( k cat : 3908–0.41 min −1 ) activities integrated into a single scaffold, and evaluate its utility in a cascade reaction. TR 2 E 2 (pH opt : 8.0–9.5; T opt : 60–65 °C) efficiently converts methyl 3-oxo-4-(2,4,5-trifluorophenyl)butanoate into 3-( R )-amino-4-(2,4,5-trifluorophenyl)butanoic acid, a crucial intermediate for the synthesis of antidiabetic drugs. The reaction proceeds through the conversion of the β-keto ester into the β-keto acid at the hydrolytic site and subsequently into the β-amino acid (e.e. >99 %) at the transaminase site. The catalytic power of the TR 2 E 2 PluriZyme was proven with a set of β-keto esters, demonstrating the potential of such designs to address bioinspired cascade reactions.

关键词： Cascade Reactions Computational Engineering Esterase Plurizyme Transaminase

来源：评论

学校读者我要写书评

暂无评论

Carbazole-Dendronized Luminescent Radicals

引用

Angewandte Chemie 2023年第28期135卷

作者： Rui Xiaotian Wataru Ota Prof. Tohru Sato Minori Furukori Prof. Yasuo Nakayama Prof. Takuya Hosokai Eri Hisamura Kazuhiro Nakamura Kenshiro Matsuda Dr. Kohei Nakao Prof. Andrew P. Monkman Prof. Ken Albrecht Department of Applied Science for Electronics and Materials Interdisciplinary Graduate School of Engineering Sciences Kyushu University 6-1 Kasuga-Koen Kasuga-shi Fukuoka 816-8580 Japan Contribution: Conceptualization (supporting) Data curation (lead) Formal analysis (lead) Funding acquisition (supporting) Investigation (equal) Visualization (equal) Writing - original draft (lead) MOLFEX Inc. Takano Nishibiraki-cho 34–4 Sakyo-ku Kyoto 606-8103 Japan Contribution: Conceptualization (supporting) Data curation (equal) Formal analysis (equal) Investigation (equal) Methodology (equal) Resources (supporting) Software (supporting) Visualization (supporting) Writing - original draft (supporting) Fukui Institute for Fundamental Chemistry Kyoto University Takano Nishibiraki-cho 34–4 Sakyo-ku Kyoto 606-8103 Japan Department of Molecular Engineering Graduate School of Engineering Kyoto University Nishikyo-ku Kyoto 615-8510 Japan Elements Strategy Initiative for Catalysts & Batteries (ESICB) Kyoto University Kyotodaigaku Katsura Nishikyo-ku Kyoto 615-8245 Japan Contribution: Conceptualization (supporting) Software (supporting) Supervision (equal) Validation (supporting) Writing - review & editing (supporting) Department of Pure and Applied Chemistry Graduate School of Science and Technology Tokyo University of Science 2641 Yamazaki Noda Chiba 278-8510 Japan National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Central 5 1-1-1 Higashi Tsukuba Ibaraki 305-8565 Japan Contribution: Data curation (supporting) Formal analysis (supporting) Investigation (supporting) Contribution: Supervision (supporting) Writing - review & editing (supporting) Contribution: Conceptualization (supporting) Data curation (supporting) Formal analysis (supporting) Funding acquisition (supporting) Supervision (equal) Writing - review & editing (equal) Institute for Materials Chemistry and Engineering Kyushu University 6-1 Kasuga-Koen Kasuga-shi Fukuoka 816-8580 J

A series of carbazole-dendronized tris(2,4,6-trichlorophenyl)methyl (TTM) radicals have been synthesized. The photophysical properties of dendronized radicals up to the fourth generation were compared systematically to understand how structure–property relationships evolve with generation. The photoluminescence quantum yield (PLQY) was found to increase with the increasing generation, and the fourth generation (G4TTM) in cyclohexane solution showed a PLQY as high as 63 % at a wavelength of 627 nm (in the deep-red region) from the doublet state. The dendron modification strategy also showed a blue-shift of the emission on increasing the generation number, and the photostability was also increased compared to the bare TTM radical.

关键词： Carbazoles Dendrimers Excited Doublet State Luminescencent Materials Stable Radicals

来源：评论

学校读者我要写书评

暂无评论

Allosteric Regulation of Aptamer Affinity through Mechano-Chemical Coupling**

引用

Angewandte Chemie 2023年第10期135卷

作者： Dr. Hao Qu Manyi Zheng Qihui Ma Dr. Lu Wang Dr. Yu Mao Michael Eisenstein Prof. Hyongsok Tom Soh Prof. Lei Zheng School of Food and Biological Engineering and Engineering Research Center of Bioprocess of Ministry of Education Hefei University of Technology Hefei 230009 China Contribution: Conceptualization (lead) Data curation (equal) Formal analysis (lead) Funding acquisition (lead) Methodology (lead) Validation (equal) Visualization (lead) Writing - original draft (equal) Writing - review & editing (equal) Contribution: Data curation (equal) Investigation (equal) Validation (lead) Writing - original draft (supporting) Contribution: Investigation (lead) Validation (supporting) Contribution: Data curation (supporting) Resources (equal) Writing - original draft (supporting) Contribution: Data curation (supporting) Methodology (supporting) Resources (supporting) Department of Electrical Engineering and Department of Radiology Stanford University Stanford CA 94305 USA Contribution: Writing - original draft (supporting) Writing - review & editing (equal) Contribution: Conceptualization (equal) Data curation (equal) Methodology (equal) Supervision (lead) Writing - original draft (equal) Writing - review & editing (equal) Contribution: Conceptualization (equal) Methodology (equal) Project administration (lead) Resources (lead) Supervision (equal) Writing - original draft (supporting) Writing - review & editing (supporting)

The capacity to precisely modulate aptamer affinity is important for a wide variety of applications. However, most such engineering strategies entail laborious trial-and-error testing or require prior knowledge of an aptamer's structure and ligand-binding domain. We describe here a simple and generalizable strategy for allosteric modulation of aptamer affinity by employing a double-stranded molecular clamp that destabilizes aptamer secondary structure through mechanical tension. We demonstrate the effectiveness of the approach with a thrombin-binding aptamer and show that we can alter its affinity by as much as 65-fold. We also show that this modulation can be rendered reversible by introducing a restriction enzyme cleavage site into the molecular clamp domain and describe a design strategy for achieving even more finely-tuned affinity modulation. This strategy requires no prior knowledge of the aptamer's structure and binding mechanism and should thus be generalizable across aptamers.

关键词： Affinity Allosterism Aptamers Mechanochemical Coupling Molecular Clamp

来源：评论

学校读者我要写书评

暂无评论

Computer-Aided Discovery of Potent Broad-Spectrum Vaccine Adjuvants

引用

Angewandte Chemie 2023年第18期135卷

作者： Juan Ma Shenqing Wang Chuanfang Zhao Xiliang Yan Quanzhong Ren Zheng Dong Jiahuang Qiu Yin Liu Qing'e Shan Ming Xu Bing Yan Sijin Liu State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 P. R. China School of Environmental Sciences University of Chinese Academy of Sciences Beijing 100049 P. R. China Contribution: Data curation (lead) Formal analysis (lead) Funding acquisition (supporting) Investigation (lead) Methodology (equal) Visualization (equal) Writing - original draft (lead) Institute of Environmental Research at Greater Bays Key Laboratory for Water Quality and Conservation of the Pearl River Delta Ministry of Education Guangzhou University Guangzhou 510006 P. R. China Contribution: Conceptualization (equal) Data curation (equal) Formal analysis (supporting) Investigation (supporting) Methodology (lead) School of Chemical Sciences University of Chinese Academy of Sciences Beijing 100049 P. R. China Contribution: Methodology (supporting) Software (lead) Visualization (lead) Contribution: Methodology (supporting) Software (lead) Visualization (supporting) Department of toxicology and sanitary chemistry School of public health Capital Medical University Beijing 100069 P. R. China Contribution: Data curation (supporting) Investigation (supporting) School of Environment Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Hangzhou 330106 P. R. China Contribution: Formal analysis (supporting) Methodology (supporting) Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250117 P. R. China Contribution: Investigation (supporting) Contribution: Supervision (supporting) Contribution: Conceptualization (equal) Funding acquisition (supporting) Methodology (supporting) Project administration (equal) Writing - review & editing (equal) Contribution: Conceptualization (equal) Funding acquisition (lead) Project administration (lead) Supervision (lead) Writing - review & editing (lead)

Adjuvants stimulate the immune system to vigorously respond to a vaccine. While current adjuvants such as aluminum salts and oil-in-water emulsions have been used for decades, they do not generate broad and long-lasting responses in many vaccines. Consequently, more potent adjuvants are needed. Here, using computer-aided molecule design and machine learning, we discovered 2 new, broad-spectrum adjuvants that can boost vaccine responses. Our library containing 46 toll-like receptor (TLR)-targeting agonist ligands were assembled on Au nanoparticles. Comprehensive in vitro , ex vivo and in vivo studies showed both leads promoted dendritic cell activation via multiple TLRs and enhanced antigen presentation to T cells. When used together with tumor-specific antigens to immunize mice against B16-OVA melanoma and 4T1-PD1 breast cancer, both adjuvants unleashed strong immune responses that suppressed tumor growth and lung metastases. Our results show computer-aided design and screening can rapidly uncover potent adjuvants for tackling waning immunity in current vaccines.

关键词： Cancer Immunotherapy Computer-Aided Design Dendritic Cell Nano Adjuvant Pattern Recognition Receptor

来源：评论

学校读者我要写书评

暂无评论

Association of dietary patterns with cognitive function in older people: Results from the Chinese longitudinal healthy longevity survey

Medicine Advances

引用

Medicine Advances 2023年第3期1卷

作者： Haiqing Zheng Huixian Li Lingcong Kong Xueli Zhang Yunfei Gao Lianting Hu Xianwen Shang Huiying Liang Medical Big Data Center Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University Guangzhou China Contribution: Conceptualization (equal) Formal analysis (equal) Visualization (equal) Writing - original draft (equal) Contribution: Conceptualization (equal) Writing - review & editing (equal) Contribution: Formal analysis (equal) Software (equal) Guangdong Eye Institute Department of Ophthalmology Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University Guangzhou China Contribution: Investigation (equal) Methodology (equal) Software (equal) Contribution: Supervision (equal) Writing - review & editing (equal) Xianwen Shang Medical Research Institute Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences) Southern Medical University Guangzhou 510080 China. Huiying Liang Medical Big Data Center Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University Guangzhou 510080 China. Contribution: Formal analysis (equal) Visualization (equal) Writing - original draft (equal) Medical Research Institute Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University Guangzhou China Lianting Hu Medical Big Data Center Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences) Southern Medical University Guangzhou 510080 China. Contribution: Methodology (equal) Project administration (equal) Validation (equal) Writing - review & editing (equal) Contribution: Conceptualization (equal) Funding acquisition (equal) Project administration (equal) Supervision (equal) Writing - review & editing (equal)

Background Dietary patterns are crucial for maintaining cognitive health among older people and can be modified through lifestyle interventions. We investigated the associations between dietary patterns, changes in these patterns over time, and cognitive function. Methods This cohort study utilized data from the Chinese Longitudinal Healthy Longevity Survey conducted between 2005 and 2014. The sample included 7472 participants (mean age: 81.45 ± 10.88 years). Dietary patterns were derived using cluster analysis. Cognitive function was assessed using the Mini-Mental State Examination (MMSE). The relationships between dietary patterns/changes, MMSE scores, changes in MMSE scores, and cognitive impairment were analyzed using the generalized estimating equation method, linear regression, and logistic regression. Results Cluster analysis identified three major dietary patterns: balanced diet, relatively balanced diet, and unbalanced diet. Mean MMSE scores decreased from 24.71 ± 7.24 in 2005 to 22.22 ± 9.29 in 2008, then increased to 24.10 ± 7.76 in 2014. Participants who adhered to balanced diet patterns exhibited significantly higher MMSE scores (1.56; 95% confidence interval: 1.34, 1.78) than those who followed unbalanced diet patterns. This association was particularly prominent among older individuals, women, individuals with no education, and underweight individuals. Participants with relatively balanced or balanced diets showed a significantly lower risk of cognitive impairment (adjusted odds ratio (OR) = 0.72, 95% CI: 0.65, 0.80; OR = 0.47, 95% CI: 0.42, 0.53, respectively) than those with unbalanced diets. Transitioning from an unhealthy diet to a healthy diet was associated with a smaller decline in MMSE scores and reduced risk of cognitive impairment. Conclusions These findings emphasize the importance of balanced or relatively balanced diets in preserving cognitive health in older individuals. These patterns relate to better MMSE scores and reduced cognitive i

关键词： cluster analysis cognitive function dietary patterns mini-mental state examination older people

来源：评论

学校读者我要写书评

暂无评论

Cover Image

引用

Developmental Dynamics 2022年第4期251卷

作者： Isabel Gross Tabea Tschigor Angelina L. Salman Fan Yang Jiankai Luo Danara Vonk Mark S. Hipp John Neidhardt Anja U. Bräuer Contribution: Conceptualization (supporting) Data curation (lead) Formal analysis (lead) Funding acquisition (supporting) Investigation (lead) Validation (equal) Visualization (lead) Writing - original draft (equal) Writing - review & editing (equal) Contribution: Data curation (supporting) Formal analysis (equal) Investigation (equal) Writing - review & editing (equal) Contribution: Investigation (equal) Validation (equal) Writing - review & editing (equal) Contribution: Methodology (equal) Resources (equal) Writing - review & editing (equal) Contribution: Formal analysis (equal) Writing - review & editing (equal) Contribution: Conceptualization (supporting) Writing - original draft (supporting) Writing - review & editing (equal) Contribution: Methodology (supporting) Resources (supporting) Writing - review & editing (equal) Contribution: Conceptualization (lead) Funding acquisition (lead) Resources (lead) Supervision (lead) Validation (equal) Writing - original draft (equal) Writing - review & editing (equal)

来源：评论

学校读者我要写书评

暂无评论

Antibiotic-Derived Radiotracers for Positron Emission Tomography: Nuclear or “Unclear” Infection Imaging?

引用

Angewandte Chemie 2022年第45期134卷

作者： Mr. Arno Christiaan Gouws Prof. Hendrik Gerhardus Kruger Dr. Olivier Gheysens Dr. Jan Rijn Zeevaart Prof. Thavendran Govender Prof. Tricia Naicker Prof. Thomas Ebenhan Catalysis and Peptide Research Unit University of KwaZulu-Natal Durban 4000 South Africa Contribution: Conceptualization (equal) Data curation (equal) Formal analysis (supporting) Investigation (lead) Methodology (lead) Project administration (supporting) Resources (equal) Visualization (lead) Writing - original draft (lead) Writing - review & editing (equal) Contribution: Data curation (equal) Methodology (lead) Project administration (equal) Supervision (supporting) Validation (equal) Writing - original draft (supporting) Writing - review & editing (equal) Department of Nuclear Medicine Cliniques Universitaires Saint-Luc and Institute of Clinical and Experimental Research Université Catholique de Louvain Brussels Belgium Contribution: Conceptualization (supporting) Data curation (supporting) Investigation (supporting) Methodology (supporting) Validation (equal) Visualization (supporting) Writing - review & editing (equal) Nuclear Medicine Research Infrastructure NPC Pretoria 0001 South Africa Radiochemistry The South African Nuclear Energy Corporation Brits 0420 South Africa Preclinical Drug Development Platform North West University Potchefstroom 2520 South Africa Contribution: Investigation (lead) Project administration (equal) Supervision (equal) Validation (equal) Writing - review & editing (equal) Department of Chemistry University of Zululand KwaDlangezwa 3886 South Africa Contribution: Conceptualization (supporting) Data curation (supporting) Formal analysis (supporting) Methodology (equal) Project administration (equal) Resources (lead) Supervision (supporting) Validation (equal) Writing - original draft (supporting) Writing - review & editing (equal) Contribution: Conceptualization (equal) Methodology (equal) Resources (equal) Supervision (lead) Validation (equal) Visualization (equal) Writing - review & editing (equal) Department of Nuclear Medicine University of Pretoria Pretoria 0001 South Africa Contribution: Conceptualization (lead)

The excellent features of non-invasive molecular imaging, its progressive technology (real-time, whole-body imaging and quantification), and global impact by a growing infrastructure for positron emission tomography (PET) scanners are encouraging prospects to investigate new concepts, which could transform clinical care of complex infectious diseases. Researchers are aiming towards the extension beyond the routinely available radiopharmaceuticals and are looking for more effective tools that interact directly with causative pathogens. We reviewed and critically evaluated (challenges or pitfalls) antibiotic-derived PET radiopharmaceutical development efforts aimed at infection imaging. We considered both radiotracer development for infection imaging and radio-antibiotic PET imaging supplementing other tools for pharmacologic drug characterization; overall, a total of 20 original PET radiotracers derived from eleven approved antibiotics.

关键词： antibiotic-derived PET imaging of infection positron emission tomography radiolabeling radiotracers

来源：评论

学校读者我要写书评

暂无评论

Thiamyxine: Struktur und Biosynthese myxobakterieller RNA-Virus Inhibitoren**

引用

Angewandte Chemie 2022年第52期134卷

作者： Patrick A. Haack Dr. Kirsten Harmrolfs Dr. Chantal D. Bader Dr. Ronald Garcia Dr. Antonia P. Gunesch Dr. Sibylle Haid Dr. Alexander Popoff Alexander Voltz Dr. Heeyoung Kim Prof. Dr. Ralf Bartenschlager Prof. Dr. Thomas Pietschmann Prof. Dr. Dr. Rolf Müller Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) and Department of Pharmacy Saarland University Saarbrücken Deutschland German center for infection research (DZIF) Braunschweig Deutschland Diese Autoren haben zu gleichen Teilen zu der Arbeit beigetragen. Contribution: Conceptualization (equal) Data curation (equal) Formal analysis (equal) Investigation (equal) Methodology (equal) Visualization (equal) Writing - original draft (equal) Writing - review & editing (equal) Contribution: Formal analysis (supporting) Methodology (supporting) Resources (supporting) Writing - review & editing (supporting) Institute of Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI) Hannover Deutschland German Center for Infection Research Hannover-Braunschweig Partner Site and Cluster of Excellence RESIST (EXC 2155) Hannover Deutschland Contribution: Data curation (supporting) Formal analysis (supporting) Investigation (supporting) Visualization (supporting) Writing - original draft (supporting) Writing - review & editing (supporting) Contribution: Data curation (supporting) Formal analysis (supporting) Investigation (supporting) Supervision (supporting) Writing - review & editing (supporting) Contribution: Investigation (supporting) Writing - review & editing (supporting) Department of Infectious Diseases Molecular Virology Heidelberg University German Center for Infection Research Heidelberg Partner Site and Division of Virus-Associated Carcinogenesis German Cancer Research Center (DKFZ) German Center for Infection Research (DZIF) Heidelberg Deutschland Contribution: Data curation (supporting) Formal analysis (supporting) Investigation (supporting) Writing - review & editing (supporting) Contribution: Data curation (supporting) Funding acquisition (supporting) Project administ

Unsere Suche nach neuen myxobakteriellen Naturstoffen führte zur Entdeckung der Thiamyxine, thiazol- und thiazolinreichen nichtribosomalen Peptid-Polyketid-Hybriden mit potenter antiviraler Aktivität. Vier Mitglieder dieser noch unbeschriebenen Naturstofffamilie wurden isoliert. Darunter auch ein offenkettiges Derivat, welches eine Glycerin-Einheit am C-Terminus trägt. Basierend auf der Analyse des Thiamyxin-Biosythesegenclusters und unterstützt durch Fütterungsexperimente mit isotopenmarkierten Vorläufersubstanzen, beschreiben wir hier neben der Strukturaufklärung der Thiamyxine ein Biosynthesemodell. Als Besonderheit ist der Einbau eines 2-(Hydroxymethyl)-4-methylpent-3-ensäure-Bausteins durch die Polyketidsynthase zu nennen, die auch eine GCN5-verwandte N-Acetyltransferase-Decarboxylase-Domäne beinhaltet. Die Thiamyxine zeigen in mit Corona-, Zika- und Dengue-Virus infizierten Zellkulturmodellen eine deutliche Hemmung von RNA-Viren. Ihre Potenz, bis zu einer halb-maximalen Hemmkonzentration von 560 nM, in Verbindung mit milderen zytotoxischen Wirkungen auf menschliche Zelllinien, indizieren ein Potenzial für die weitere Entwicklung der Thiamyxine.

关键词： Antivirale Wirkstoffe Biosynthese Depsipeptide Naturstoffe Strukturaufklärung

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：