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SSRN 2024年

作者： Hao, Qi Lei, Yongdong Li, Runze Ma, Lingzhen Zheng, Huihui Deng, Xiaorong Zhang, Jian Ministry of Agriculture and Rural Affairs School of Food Science and Technology Shihezi University Xinjiang Shihezi832003 China Key Laboratory for Food Nutrition and Safety Control Xinjiang Production and Construction Corps School of Food Science and Technology Shihezi University Xinjiang Shihezi832003 China Engineering Research Center of Storage and Processing of Xinjiang Characteristic Fruits and Vegetables Ministry of Education School of Food Science and Technology Shihezi University Xinjiang Shihezi832003 China Food Quality and Testing Center Ministry of Agriculture and Rural Affairs Xinjiang Academy of Agricultural and Reclamation Science Xinjiang Shihezi832003 China

Pretreatment for dairy processing sometimes requires freezing and thawing to ensure a steady supply of fresh raw milk. On this basis, a series of studies were conducted to ascertain the impact of freeze‒thaw cycles on the physicochemical stability and nutritional quality of camel milk. The findings demonstrated that while the lipid content and apparent viscosity of camel milk were significantly reduced, the acidity and quantity of short- and medium-chain free fatty acids were enhanced by repeated freeze‒thaw cycles. Freeze‒thaw cycles have been demonstrated to result in an increase in protein oxidation in camel milk, which in turn has been observed to give rise to a shift in the secondary structure towards random coils. The particle size distribution and microstructure of camel milk exhibited aggregation of the droplets, which consequently reduced the physical stability of the milk emulsion. The results of this study may help to deepen our understanding of the quality characteristics of raw camel milk materials while providing methodological references and scientific support for the storage of raw camel milk on small farms. © 2024, The Authors. All rights reserved.

关键词： Particle size analysis

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The Synergistic Effects and Mechanism of Action of the Combination of Nisin and Salicylic Acid Against Staphylococcus Aureus and its Application

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SSRN 2024年

作者： Li, Runze Hao, Qi Yu, Han Ma, Lingzhen Zheng, Huihui Deng, Xiaorong Lei, Yongdong Zhang, Jian School of Food Science and Technology Shihezi University Shihezi Xinjiang832000 China School of Food Science and Technology Shihezi University Shihezi Xinjiang832000 China Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps School of Food Science and Technology Shihezi University Shihezi Xinjiang832000 China Engineering Research Center of Storage and Processing of Xinjiang Characteristic Fruits and Vegetables Ministry of Education School of Food Science and Technology Shihezi University Shihezi Xinjiang832000 China Center for Food Quality Supervision & Testing Ministry of Agriculture Xinjiang Academy of Agricultural and Reclamation Science Shihezi Xinjiang832000 China

In this study, the synergistic effects of nisin and salicylic acid (SCA) on Staphylococcus aureus and its potential application as food preservatives were investigated. The antimicrobial activities of nisin (MIC=500 μg/mL) and SCA (MIC=250 μg/mL) when used alone were determined. The FICI was 0.5, which confirmed that nisin and SCA had synergistic antibacterial effects against S. aureus. Based on these findings, the synergistic mechanism of nisin and SCA was further explored. This combination enhanced cell membrane destruction, increased the release of cell contents, reduced ATP synthesis, and inhibited biofilm synthesis. In addition, the combination demonstrated excellent DNA binding ability. The combination inhibited S. aureus infection in whole/skimmed ultra-high temperature sterilized milk at 25°C. Therefore, the combination of nisin and SCA is expected to be a promising strategy for food preservation. © 2024, The Authors. All rights reserved.

关键词： Salicylic acid

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A Freshness Indicator Film of Bighead Carp Heads Using Anthocyanin Encapsulated by Whey Protein (Wp)-Propylene Glycol Alginate (Pga) Nanoparticles

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SSRN 2023年

作者： Shi, Ge Shi, Ce Luo, Yongkang Hong, Hui Tan, Yuqing Jia, Zhixin Yang, Xinting Ying, Xiaoguo Information Technology Research Center Beijing Academy of Agriculture and Forestry Sciences Beijing100097 China Beijing Laboratory for Food Quality and Safety College of Food Science and Nutritional Engineering China Agricultural University Beijing100097 China Key Laboratory of Cold Chain Logistics Technology for Agri-product Ministry of Agriculture and Rural Affairs Beijing100097 China National Engineering Laboratory for Agri-product Quality Traceability Beijing Academy of Agricultural and Forestry Sciences Beijing100097 China Center of Food Colloids and Delivery for Functionality College of Food Science and Nutritional Engineering China Agricultural University Beijing China Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood Collaborative Innovation Center of Seafood Deep Processing College of Food and Pharmacy Zhejiang Ocean University Zhoushan316000 China

Freshness indicator films were prepared with anthocyanins (ACNs)-encapsulated whey protein (WP)-propylene glycol alginate (PGA) nanoparticles and polyvinyl alcohol (PVA) and then applied to the freshness indication of the bighead carp heads. The characteristics of films with and without ACNs encapsulated were compared. ACNs was fixed in the film successfully according to the analysis of SEM and ATR-FTIR. The results of light transmission, water vapor transmission rate, and stability analysis indicated that the film possessed good light blocking property, water barrier property, and stability with ACNs encapsulated. The freshness indicator films changed from rosy red to gray in the application experiments of bighead carp heads at 4 and 25 °C, and the color change (ΔE) of the films had good linear correlations with the change of freshness indexes. Therefore, the encapsulation of ACNs has provided a more stable and accurate freshness indication of bighead carp heads during storage and transportation. © 2023, The Authors. All rights reserved.

关键词： Proteins

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Investigation of the Antioxidative Potential of Meretrix Meretrix Linnaeus Derived Peptides from Simulated Gastrointestinal Digestion: In Vitro and in Silico Insights

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SSRN 2023年

作者： Du, Chao Hui, Zhenzhen Li, Na Liu, Kaiyue Wang, Yong Wang, Ping Sun, Shuyang School of Food Engineering Ludong University China BioNanotechnology Institute Ludong University China Yantai Key Laboratory of Nanoscience and Technology for Prepared Food China Yantai Engineering Research Center of Green Food Processing and Quality Control 186 Middle Hongqi Road Shandong Province Yantai264025 China Blood Transfusion Department Changle People's Hospital 278 Limin Street Changle County Shandong Province Weifang262400 China

This investigation sought to identify antioxidative peptides derived from Meretrix meretrix Linnaeus (MML) throughout simulated gastrointestinal digestion (SGD) via in vitro and in silico strategies. The study demonstrated that hydrolysates from MML captured at various SGD phases showcased robust antioxidative activity. These hydrolysates also displayed a protective role against hydrogen peroxide-induced stress in Raw 264.7 cells. Notably, hydrolysates sourced from the intestinal phase of digestion (MMLHs-I) markedly inhibited the reduction of cell viability, limited the generation of reactive oxygen species under oxidative stress conditions, and promoted the activity of key antioxidant enzymes including superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, thus amplifying the cells' antioxidative defense system. Through nLC-MS/MS analysis, 105 peptides tracing back to 18 parent proteins were pinpointed in MMLHs-I, with 18 of these peptides previously reported in the BIOPEP-UWM database. Subsequent in silico analysis, alongside empirical antioxidant activity assays, pinpointed the peptide WLV as an exceptional antioxidative agent, demonstrating an IC50 of 0.125 mg/mL in the ABTS assay and an impressive 604 μM Trolox/μg in the ORAC assay. The outcomes highlight MML's promise as a novel source of antioxidative peptides, positioning peptide WLV as a potentially valuable addition to functional foods designed to combat oxidative stress. © 2023, The Authors. All rights reserved.

关键词： Peptides

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Observed Effects on Very Early Pregnancy Linked to Ambient PM_(2.5) Exposure in China among Women Undergoing In Vitro FertilizationEmbryo Transfer

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Environment & Health 2024年第12期2卷 918-928页

作者： Changxin Lan Yichun Guan Haining Luo Xiaoling Ma Yihua Yang Hongchu Bao Cuifang Hao Xiaojin He Han Zhang Ning Gao Weinan Lin Mengyuan Ren Tianxiang Wu Chao Wang Xiaoqing Ni Chunyan Shen Jianrui Zhang Junfang Ma Rui Zhang Yin Bi Lili Zhuang Ruichao Miao Ziyi Song Tong An Zhengteng Liu Bo Pan Mingliang Fang Jing Liu Zhipeng Bai Fangang Meng Yuanchen Chen Xiaoxia Lu Yuming Guo Yunxia Cao Qun Lu Bin Wang Department of Epidemiology and Biostatistics School of Public HealthPeking UniversityBeijing 100191China Institute of Reproductive and Child Health Key Laboratory of Reproductive HealthNational Health and Family Planning Commission of the People’s Republic of ChinaBeijing 100191China Department of Reproductive Medicine the Third Affiliated Hospital of Zhengzhou UniversityZhengzhou 450003China Reproductive Medicine Center Tianjin Central Hospital of Gynecology ObstetricsMaternity Hospital of Nankai UniversityTianjin 300052China The First Hospital of Lanzhou University the First Clinical Medical School of Lanzhou UniversityLanzhou 730000China Guangxi Reproductive Medical Center the First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China Reproductive Medicine Centre Yuhuangding Hospital of YantaiAffiliated Hospital of Qingdao UniversityYantai 264000China Qingdao Women and Children’s Hospital Affiliated Hospital of Qingdao UniversityQingdao 266555China Reproductive Medicine Center Department of Obstetrics and GynecologyShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Department of Obstetrics and Gynecology the First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Engineering Research Center of Biopreservation and Artificial Organs Ministry of EducationHefei 230022China Medical Center for Human Reproduction Beijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China Yunnan Provincial Key Lab of Soil Carbon Sequestration and Pollution Control Faculty of Environmental Science and EngineeringKunming University of Science and TechnologyKunming 650500China MOE Key Laboratory of Environmental Remediation and Ecosystem Health College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China Beijing Neurosurgical Institute Beijing Tiantan Hos

The adverse effect of ambient PM_(2.5) exposure on very early pregnancy(VEP)remains controversial among epidemiological studies but is supported by toxicological *** adopted a multicenter retrospective cohort of 141,040 cycles to evaluate the effect of PM_(2.5) exposure on the VEP using the in vitro fertilization and embryo transfer platform and high-resolution PM_(2.5) data in *** first investigated the association between PM_(2.5) exposure 1 week before and 1 week after the embryo transfer date and *** average PM_(2.5) concentrations of the 2 weeks were approximately 47μg/m^(3).The pooled results revealed a negative association between women’s accumulated PM_(2.5) exposure during the 2 weeks near the day of embryo transfer and success odds of VEP with the relative risk of 0.999(95%CI:0.997-0.999)at each increase of 10μg/*** women with the fresh cycle or one transplanted embryo were considered as a vulnerable ***,seven periods for the fresh cycle and five periods for the frozen cycle from 85 days before oocyte retrieval to the day of gestational sac detection by ultrasound detection were *** these exposure periods,no association between the average PM_(2.5) exposure and VEP risk was *** study provided large-scale population evidence for the association between PM_(2.5) exposure near embryo transfer day and VEP and identified vulnerable populations among women undergoing in vitro fertilization-embryo transfer.

关键词： Fine particulate matter Very early pregnancy IVF-ET Biochemical pregnancy Clinical pregnancy

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Enhancing Antioxidant Capacity and controlling Post-Acidification Potential in Yogurt Fermentation: A Novel Approach Using Thermosonication Microstress and Pineapple Peel Extracts

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SSRN 2024年

作者： Zhang, Xiaohui Zheng, Yuanrong Zhou, Changyu Cao, Jinxuan Zhang, Yifeng Wu, Zhen Pan, Daodong Cai, Zhendong Xia, Qiang College of Food Science and Engineering Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition State Key Laboratory for Managing Biotic and Chemical Threats the Quality and Safety of Agro-products Ningbo University Ningbo315211 China State Key Laboratory of Dairy Biotechnology Shanghai Engineering Research Center of Dairy Biotechnology Dairy Research Institute Bright Dairy & Food Co. Ltd. Shanghai200436 China School of Food and Health Beijing Technology and Business University 11 Fucheng Road Beijing100048 China Department of Food Safety and Health School of Advanced Agriculture Sciences Peking University Beijing100871 China

This work investigated the effects of the combined use of thermosonication-preconditioned lactic acid bacteria (LAB) with the addition of pineapple peel extracts (UU) on the post-acidification potential, physicochemical and functional features of yogurt products, aimed at achieving prolonged preservation and enhancing functional attributes. Accordingly, physical-chemical features, probiotic evaluation as revealed by adhesion properties, and sensory assessment, acidification curves, the contents of major organic acids, and the antioxidant activities of the differentially processed yogurts during refrigeration were characterized. Following a 14-day chilled storage process, UU group exhibited acidity levels 0.5-2 oT lower than the control group and a higher lactose content of 0.07 mg/ml as well as unmodified adhesion potential, indicating that the proposed combination method efficiently inhibited post-acidification and delayed lactose metabolism without leading to significant impairment of the probiotic properties. Furthermore, the total phenolic content of UU-treated samples was 98 μg/mL, 1.78 times higher than that of the control. The IC50 values of DPPH and ABTS radical scavenging activities of the UU group were 68.21 μg/mL and 61.65 μg/mL, significantly lower than those of the NN group (165.59 μg/mL and 147.98 μg/mL), respectively, and the FRAP value increased significantly to 2.22 mmol/L. Observations by fluorescence inverted microscopy demonstrated the obvious adhesion phenomenon with no significant difference found among differentially prepared yogurts. The results of targeted metabolomics indicated the proposed combination strategy significantly modified the microbial metabolism, leading to the delayed utilization of lactose and the inhibited conversion into glucose, as well as the decreased lactic acid production and a notable shift towards the formation of relatively weak acids such as succinic acid and citric acid. This study confirmed the feasibility of the

关键词： Metabolism

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Classification and Quantification of Adulterants in Alaska Pollock Based on Fourier Transform Near-Infrared Spectroscopy with Chemometrics

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SSRN 2023年

作者： Wang, Zhouping Feng, Shijia Qi, Shou Chen, Chen Khan, Imran Mahmood Gu, Qianhui Zhang, Yin State Key Laboratory of Food Science and Resources Jiangnan University Wuxi214122 China School of Food Science and Technology Jiangnan University Wuxi214122 China National Engineering Research Center for Functional Food Jiangnan University Jiangsu Wuxi214122 China International Joint Laboratory on Food Safety Jiangnan University Wuxi214122 China Key Laboratory of Meat Processing of Sichuan Chengdu University Chengdu610106 China Collaborative Innovation Center of Food Safety and Quality Control of Jiangsu Province Jiangnan University Wuxi214122 China College of Ocean Food and Biological Engineering Jimmie University Jimei University Jimei District Fujian Province Xiamen City361021 China

In this study, the Fourier transform near-infrared spectroscopy (FT-NIRS) was combined with chemometrics methods to rapidly and non-destructively evaluate the adulteration levels of Alaska Pollock surimi. The principal component analysis (PCA) were performed for feature dimensionality reduction. Partial least squares discriminant analysis (PLS-DA), random forest (RF) and k-nearest neighbors (KNN) were used to establish the classification model. The chemometric tools include partial least squares regression (PLSR), support vector regression (SVR) and back-propagation neural network (BPNN) were utilized to quantify the level of adulteration. For qualitative analysis, the random forest classification model had the most suitable prediction results with accuracy, precision, recall and F1-score of =92.18%, 98.04%, 92.59%, 95.29%, respectively. The SVR with Standard Normal Variate (SNV) pre-treatment was the optimal prediction model for quantitative analysis with the RC2 of 0.941, RMSEC of 0.060, RP2 of 0.946 and RMSEP of 0.062. The present study provides a reliable basis for rapid and non-destructive detection of Alaska Pollock content in surimi and has great potential for determining other seafood species. © 2023, The Authors. All rights reserved.

关键词： Nearest neighbor search

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Tmt-Based Quantitative Proteomic Analysis of Antifungal Mechanism of Phenyllactic Acid Against Mucor Spore

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SSRN 2024年

作者： Zhang, Chaoqi Wang, Yunfan Guo, Mingmei Kong, Yanhui Fan, Xinguang Sun, Shuyang Du, Chao Gong, Hansheng School of Food Engineering Ludong University 186 Middle Hongqi Road Shandong Province Yantai264025 China Yantai Key Laboratory of Nanoscience and Technology for Prepared Food 186 Middle Hongqi Road Shandong Province Yantai264025 China Yantai Engineering Research Center of Green Food Processing and Quality Control 186 Middle Hongqi Road Shandong Province Yantai264025 China Mudan District Mudan Street Sub-district Office Shandong Province Heze274000 China Yantai Landscape Construction and Maintenance Center Shandong Province Yantai264000 China China Agricultural University China

The efficacy of PLA in inhibiting Mucor spore growth and its mechanisms of action were investigated in this study. PLA treatment suppressed Mucor spore growth in a dose-dependent manner, with a minimum inhibitory concentration of 12 mmol/L. Morphological analysis revealed that PLA caused nuclear chromatin condensation, DNA fragmentation, and severe ultrastructural damage. Additionally, results of flow cytometry showed that PLA induced phosphatidylserine externalization, mitochondrial membrane potential depolarization, and intracellular reactive oxygen species accumulation in Mucor spore cells. TMT-based proteomic analysis identified 1,248 differentially expressed proteins (DEPs) in Mucor spores treated with 24 mmol/L PLA, which were primarily involved in energy and material metabolism, including oxidative phosphorylation, glycolysis, the citrate cycle, and the biosynthesis and metabolism of carbon and amino acids. These findings elucidate the antifungal mechanisms of PLA against Mucor spores and provide valuable insights into the potential application of PLA in food preservation. © 2024, The Authors. All rights reserved.

关键词： Fungi

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Differential neutrophil responses in murine following intraperitoneal injections of Escherichia coli and Staphylococcus aureus

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Heliyon 2024年第22期10卷 e40281页

作者： Zhu, Yanyan Luo, Jingya Xia, Xianzhu Feng, Hao Zhao, Pingsen Department of Laboratory Medicine Yuebei People's Hospital Affiliated to Shantou University Medical College Shaoguan 512025 China Laboratory for Diagnosis of Clinical Microbiology and Infection Yuebei People's Hospital Affiliated to Shantou University Medical College Shaoguan 512025 China Research Center for Interdisciplinary & High-Quality Innovative Development in Laboratory Medicine Shaoguan 512025 China Shaoguan Municipal Quality Control Center for Laboratory Medicine Yuebei People's Hospital Affiliated to Shantou University Medical College Shaoguan 512025 China Shaoguan Municipal Quality Control Center for Surveillance of Bacterial Resistance Shaoguan 512025 China Shaoguan Engineering Research Center for Research and Development of Molecular and Cellular Technology in Rapid Diagnosis of Infectious Diseases and Cancer Shaoguan 512025 China College of Animal Science and Technology Jilin Agricultural University Changchun 130118 China Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun 130122 China Jiaxing University Master Degree Cultivation Base Zhejiang Chinese Medical University Jiaxing China

Objective: This study aimed to investigate the proportion of neutrophils among leukocytes, in various tissues following intraperitoneal injection of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in mice. Methods: Twelve specific-pathogen free (SPF) male mice, aged eight weeks, were segregated into three groups, each containing four mice. Two of these groups were subjected to intraperitoneal injections of E. coli and S. aureus, both in high concentrations, to establish mouse models of inflammation. The remaining group, which received an intraperitoneal injection of phosphate buffered saline (PBS), served as the control group. Observe the mice every half hour. Then mice were anesthetized, and samples from peripheral blood, liver, and brain tissues were carefully collected nearing death. These samples underwent a digestion process to produce single-cell suspensions. Subsequently, these suspensions were stained with fluorescent antibodies targeting CD45, Ly6G, and CD11b. A flow cytometric analyzer was then employed to enumerate and compare the neutrophil alterations across each group (Fig. 1). Results: The results indicated a significant variation in the ratio of CD11b⁺ Ly6G⁺ neutrophils to CD45⁺ leukocytes among the groups. In peripheral blood, the control group showed a neutrophil proportion of approximately 1.44 %, while the E. coli and S. aureus groups exhibited increased proportions of 6.53 % and 3.82 %, respectively. In liver tissue, a marked elevation was observed in the experimental groups, with ratios of 19.20 % and 20.40 % for E. coli and S. aureus, respectively, compared to 1.64 % in the control. In brain tissue, the increments were more modest but noticeable, with the experimental groups showing 2.40 % and 1.11 % in contrast to 0.13 % in the control group. Conclusions: These findings suggest neutrophils are involved in the response after intraperitoneal injection of E. coli and S. aureus, with marked differ

关键词： Escherichia coli Flow cytometry Inflammation Neutrophils Staphylococcus aureus

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Deciphering the preservative effects of protamine from Xinjiang Coregonus peled on grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage: a perspective from the microbiome

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International Journal of Food Microbiology 2025年 440卷 111222-111222页

作者： Zhang, Rong Gao, Yanan Fan, Xiaoji Zhu, Song Wang, Tingzhang Gao, Lili Zhao, Xueqin Ma, Zhuang Fan, Yiling Jiang, Dan Liu, Shuyan Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing 100193 China Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province Hangzhou 310012 China Institute of Western Agriculture the Chinese Academy of Agricultural Sciences/Xinjiang Engineering Technology Research Center for Exploration and Quality Evaluation of Characteristic Agricultural and Livestock Product Resources Changji 831100 China National Medical Products Administration Key Laboratory for Testing Technology of Pharmaceutical Microbiology Shanghai Quality Inspection and Testing Center for Innovative Biological Products Shanghai Institute for Food and Drug Control Shanghai 201203 China College of Food Science and Engineering Dalian Ocean University Dalian 116023 China Xinjiang Saihu Fishery Technology Develop. Co. Ltd Bortala Mongolian Autonomous Prefecture 833400 China Zibo Institute for Digital Agriculture and Rural Research Zibo 255000 China

Protamine is a natural cationic peptide which has been utilized as a food preservative, since its antibacterial capability against a wide range of Gram-negative and -positive bacteria. Despite the antibacterial mechanism has been well uncovered, its role in complex food microbial communities is largely elusive. In the present study, grass carp (Ctenopharyngodon idellus) fillets were treated with protamine and a protamine-based compound preservative, and stored at 4 °C for 15 days. The pH, total viable count (TVC), and total volatile base nitrogen (TVB-N) were monitored during the refrigerated storage, as well as the bacterial communities by 16S rRNA amplicon sequencing. Protamine, especially the protamine compound preservative, reduced the pH, TVC and TVB-N during the storage, and prolonged the shelf life of fish fillets. Bacterial communities on fillets during the refrigerated storage challenged by protamine and its compound preservative significantly differed from that in control. Prevalent genera including Aeromonas, Pseudomonas, and Myroides were significantly altered by protamine treatment. A group of 18 genera were closely associated with the antibacterial activity of protamine. The fillets treated with protamine possessed a more complex and stable bacterial community. Our study depicted a dynamic alteration of bacterial communities during the refrigerated storage of fish fillets, and offered an insight into the understanding of the antibacterial mechanisms of protamine from a microbiota perspective. © 2025 Elsevier B.V.

关键词： Compound preservative Food preservative Grass carp Microbiome Protamine Refrigerated storage

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