Probiotic Lactobacillus brevis CLB3 prevents azoxymethane/dextran sulfate sodium-induced colon carcinogenesis in mice by reducing amino acid transport and IL-17A levels and repressing the IL-6/AKT/p-STAT3 signaling pathway

作     者：Weiyi Qian Yong Zhang Yan Long Wei Yang Rui Hu Jinping Li Yan Leng Xinjie Liu Qing X.Li Xiangyuan Wan Xun Wei 钱伟祎;张勇;龙艳;杨薇;胡瑞;李金萍;冷燕;刘欣洁;李庆孝;万向元;魏珣

作者机构：Zhongzhi International Institute of Agricultural BiosciencesShunde Graduate SchoolResearch Center of Biology and AgricultureUniversity of Science and Technology BeijingBeijingChina Beijing Beike Institute of Precision Medicine and Health TechnologyBeijingChina Beijing Engineering Laboratory of Main Crop Bio-Tech BreedingBeijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing Solidwill Sci-Tech Co.Ltd.BeijingChina College of Basic ScienceTianjin Agricultural UniversityTianjinChina Department of Molecular Biosciences and BioengineeringUniversity of Hawaii at ManoaHonoluluHawaiiUSA 

出 版 物：《Food Quality and Safety》 (食品品质与安全研究（英文版）)

年 卷 期：2022年第6卷第4期

页      面：664-677页

核心收录：

学科分类：08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：supported by the National Key Research and Development Program of China(2018YFC2000504) the Fundamental Research Funds for the Central Universities(No.06500060) the Beijing Municipal Science and Technology Project(No.Z181100009318004),China the USDA HATCH Project(HAW05020H),USA 

主　　题：Lactobacillus brevis CLB3 probiotic gut microbes colorectal cancer amino acid transporter 

摘      要：Amino acid intake plays a crucial role in the Warburg effect of *** microbes can regulate intestinal amino acid ***,it is still unknown whether probiotic therapy can protect the host from intestinal tumor invasion by reducing amino acid *** in vitro methods,three acid-tolerant strains from fermented pickles were screened *** azoxymethane/dextran sulfate sodium-induced colon cancer models,we evaluated the therapeutic effects of Lactobacillus brevis CLB3,Lactobacillus plantarum XLP,and Lactobacillus johnsonii CM on model *** functional mechanisms were further explained through anatomy section,quantitative reverse transcription polymerase chain reaction(qRT-PCR),Western blot,and immunohistochemical staining analyses as well as database mining and gut *** Lactobacillus brevis(***)CLB3 treatment significantly improved the clinical signs and symptoms of colon cancer,alleviated colon damage,and inhibited colon carcinogenesis in *** addition,this treatment significantly increased gut cultivable Lactobacillus abundance,inhibited the expression and translation levels of the tumor metabolism-related solute carrier(SLC)amino acid transporter including SLC7A5 and SLC7A11,lowered circulating interleukin-6(IL-6)and interleukin-17A(IL-17A)levels,and improved the accumulation of tumor-infiltrating lymphocytes and cancer proliferation *** findings suggest that *** CLB3 can reduce amino acid transport,inhibit mammalian target of rapamycin(mTOR)signaling and enhance intestinal anti-tumor immune responses,which provides a potential targeting amino acid transporter strategy for preventing colorectal cancer.