Composition of fatty acid methyl ester in microalgae Chlorella vulgaris: Comparison between various methods of harvesting, extraction and transesterification

作     者：Mat Yasin, N.H. Mohd Yusop, M.A. Awang Kechik, N.A.A. Zahuri, A.Z. 

作者机构：Faculty of Chemical and Process Engineering Technology College of Engineering Technology Universiti Malaysia Pahang 26300 Gambang Pahang Malaysia 

出 版 物：《Materials Today: Proceedings》 (Mater. Today Proc.)

年 卷 期：2023年

核心收录：

学科分类：0710[理学-生物学] 0828[工学-农业工程] 0817[工学-化学工程与技术] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0703[理学-化学] 

基　　金：Heartful thanks and appreciation to Universiti Malaysia Pahang (UMP) for providing an Internal Fundamental Research Grant (RDU1903138) for the financial support throughout the research work 

主　　题：Microalgae 

摘      要：The aim of the study was to identify which of methods produces the highest yield of fatty acid methyl ester (FAME) from microalgae. Different methods of harvesting (centrifugation, Cen & coagulation, Co), extraction (Test Tube, TT & Soxhlet, Sox) and transesterification (direct transesterification, d-trans & extraction transesterification, ext-trans) were considered. The procedures of this research consists of microalgae cultivation, harvesting of biomass and extraction of oil to obtain the lipid. The further process of converting lipid into FAME is followed by transesterification process. The FAME compositions of Chlorella vulgaris were analyzed using Gas chromatography mass spectrometry (GC-MS). The main components were consists of palmitic, stearic and oleic acids for both transesterification methods. However, FAME extracted from d-trans contained more components (linoleic and linolenic acid) than ext-trans. Furthermore, the highest amount of FAME gained by ext-trans was produced by the method of centrifugation-test tube (Cen-TT). Although Cen-TT produced the highest amount of FAME for ext-trans, it still cannot meet the highest amount of FAME produced by d-trans. Therefore, the Cen-TT method was selected to be the best microalgae harvesting and extraction method in ext-trans meanwhile the most efficient transesterification method was d-trans. © 2023 Elsevier Ltd. All rights reserved.