MITOGEN AND ANTIGEN-INDUCED B-CELL AND T-CELL RESPONSES OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM THE HARBOR SEAL (PHOCA-VITULINA)

作     者：DESWART, RL KLUTEN, RMG HUIZING, CJ VEDDER, LJ REIJNDERS, PJH VISSER, IKG UYTDEHAAG, FGCM OSTERHAUS, ADME 

作者机构：SEAL REHABIL & RES CTR9968 AG PIETERBURENNETHERLANDS INST FORESTRY & NAT RES1790 AB DEN BURGNETHERLANDS 

出 版 物：《VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY》 (兽医免疫学和免疫病理学)

年 卷 期：1993年第37卷第3-4期

页      面：217-230页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 0906[农学-兽医学] 09[农学] 

主　　题：Ab antibody AFC antibody forming cells Ag antigen CDV canine distemper virus CM culture medium Con A concanavalin A cpm counts per minute ELISA enzyme linked immunosorbent assay FBS fetal bovine serum 3 H-Trd tritium labeled thymidin IU international units LF flocculation unit LPS lipopolysaccharide from Salmonella typhimurium PBMC peripheral blood mononuclear cells PBS phosphate buffered saline PDV phocine distemper virus PHA phytohaemagglutinin PWM pokeweed mitogen rhIL-2 recombinant human interleukin-2 RV rabies virus antigen SD standard deviation SI stimulation index TT tetanus toxoid 5-BCIP 5-bromo-4-chloro-3-indolyl phosphate 

摘      要：In vitro assays were developed for studies concerning the functioning of the immune system of the harbour seal (Phoca vitulina). Proliferative responses of peripheral blood mononuclear cells (PBMC) were measured after stimulation with different concentrations of the mitogens concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA) or lipopolysaccharide from Salmonella typhimurium (LPS). Con A and PWM induced strong proliferative responses, while PHA and LPS induced comparatively low proliferative responses. Responses of mitogen stimulated PBMC to recombinant human interleukin-2 (rhIL-2) and in vitro immunoglobulin production by mitogen stimulated PBMC were measured to discriminate between stimulation of T cells and B cells. It was found that Con A and PHA stimulate phocine T cells, PWM stimulates both T cells and B cells and LPS predominantly stimulates phocine B cells. Antigen-specific immune responses were measured after immunization of seals with an inactivated rabies vaccine and/or with tetanus toxoid. Antigen-specific proliferation of PBMC and the presence of antigen-specific antibody forming cells were demonstrated for both antigens in the PBMC of immunized animals. The responses measured in vitro correlated well with the development of specific serum antibody titers to these antigens.