Concurrently bioprinted scaffolds with autologous bone and allogeneic BMSCs promote bone regeneration through native BMSC recruitment

作     者：Yu Huan Hongqing Chen Dezhi Zhou Xin He Sanzhong Li Xiuquan Wu Bo Jia Yanan Dou Xiaowei Fei Shuang Wu Zhou Fei Tao Xu Fei Fei 郇宇;陈红庆;周德志;何鑫;李三中;武秀权;贾博;豆雅楠;费晓炜;吴霜;费舟;徐弢;费霏

作者机构：Department of NeurosurgeryXijing HospitalAir Force Medical UniversityXi’an 710032China Department of NeurosurgeryGeneral Hospital of Northern Theater CommandShenyang 110840China Biomanufacturing and Rapid Forming Technology Key Laboratory of BeijingDepartment of Mechanical EngineeringTsinghua UniversityBeijing 100084China Key Laboratory for Advanced Materials Processing TechnologyMinistry of EducationDepartment of Mechanical EngineeringTsinghua UniversityBeijing 100084China Department of Precision Medicine and HealthcareTsinghua Berkeley Shenzhen InstituteShenzhen 518055China Center for Bio-intelligent Manufacturing and Living Matter BioprintingResearch Institute of Tsinghua University in ShenzhenShenzhen 518057China Department of OphthalmologyXijing HospitalAir Force Medical UniversityXi’an 710032China 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造(英文))

年 卷 期：2025年第8卷第1期

页      面：85-99,I0042,I0043页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0802[工学-机械工程] 080201[工学-机械制造及其自动化] 

基　　金：supported by the Science and Technology Development Fund of the Fourth Military Medical University(No.2016XB051) the Military Medical Promotion Plan of the Fourth Military Medical University(No.2016TSA-005) the Science and Technology Program of Guangzhou(No.201604040002) the Youth Development Project of Air Force Medical University(No.21QNPY072) the Xijing Hospital Booster Program(No.XJZT24CZ10) 

主　　题：Concurrent 3D bioprinting Cranioplasty Autologous bone particles Allogeneic mesenchymal stem cells Recruitment 

摘      要：Autologous bone marrow-derived mesenchymal stem cells(BMSCs)have been shown to promote osteogenesis;however,the effects of allogeneic BMSCs(allo-BMSCs)on bone regeneration remain ***,we explored the bone regeneration promotion effect of allo-BMSCs in 3D-printed autologous bone particle(ABP)***,we concurrently printed scaffolds with polycaprolactone,ABPs,and allo-BMSCs for appropriate support,providing bioactive factors and seed cells to promote *** vitro studies showed that ABP scaffolds promoted allo-BMSC osteogenic *** vivo studies revealed that the implantation of scaffolds loaded with ABPs and allo-BMSCs into canine skull defects for nine months promoted *** experiments suggested that only a small portion of implanted allo-BMSCs survived and differentiated into vascular endothelial cells,chondrocytes,and *** implanted allo-BMSCs released stromal cell-derived factor 1 through paracrine signaling to recruit native BMSCs into the defect,promoting bone *** study contributes to our understanding of allo-BMSCs,providing information relevant to their future application.