sRNA113 regulates Pseudomonas plecoglossicida motility to affect immune response against infection in pearl gentian grouper

作     者：Li He Mei-Qin Mao Ling-Min Zhao Qi Li Hui Ge Jiao-Nan Zhang Jiao-Lin Zhang Qing-Pi Yan Li He;Mei-Qin Mao;Ling-Min Zhao;Qi Li;Hui Ge;Jiao-Nan Zhang;Jiao-Lin Zhang;Qing-Pi Yan

作者机构：Fisheries CollegeJimei UniversityXiamenFujian 361021China Fisheries Research Institute of FujianXiamenFujian 361000China Key Laboratory of Special Aquatic Feed for FujianFujian Tianma Technology Company LimitedFuzhouFujian 350308China 

出 版 物：《Zoological Research》 (动物学研究(英文))

年 卷 期：2025年第46卷第1期

页      面：152-164页

核心收录：

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China (32373181) National Key Research and Development Program (2023YFD2400700) Science and Technology Plan Project of Fujian Province (2022L3059) High-quality Development of Marine and Fishery Industry Special Fund Project of Fujian Province (FJHYF-L-2023-5) Open Fund of Fujian Province Key Laboratory of Special Aquatic Formula Feed (TMKJZ2302) 

主　　题：Pseudomonas plecoglossicida sRNA Virulence Pearl gentian grouper Immune response 

摘      要：Small RNAs(sRNAs)are a class of molecules capable of perceiving environmental changes and exerting posttranscriptional regulation over target gene expression,thereby influencing bacterial virulence and host immune *** plecoglossicida is a pathogenic bacterium that poses a significant threat to aquatic animal ***,the regulatory mechanisms of sRNAs in *** remain *** study focused on sRNA113,previously identified as a potential regulator of the fliP gene,a key component of the lateral flagellar type III secretion *** investigate the effects of sRNA113on *** virulence,as well as its role in regulating pathogenic processes and host immune responses,mutant strains lacking this sRNA were generated and *** of sRNA113 resulted in the up-regulation of lateral flagellar type III secretion system-related genes in ***,which enhanced bacterial swarming motility,biofilm formation,and chemotaxis ability in *** vivo infection experiments with pearl gentian grouper revealed that sRNA113 deletion enhanced the pathogenicity of *** heightened virulence was attributed to the up-regulation of genes associated with the lateral flagellar type III secretion system,resulting in higher bacterial loads within host *** amplification of pathogenic activity intensified tissue damage,disrupted immune responses,and impaired the ability of the host to clear infection,ultimately leading to *** findings underscore the critical role of sRNA113 in regulating the virulence of *** and its interaction with host immune *** study provides a foundation for further exploration of sRNAmediated mechanisms in bacterial pathogenesis and hostpathogen interactions,contributing to a deeper understanding of virulence regulation and immune evasion in aquatic pathogens.