CmBBX28-CmMYB9a Module Regulates Petal Anthocyanin Accumulation in Response to Light in Chrysanthemum

作     者：Zhou, Li-Jie Peng, Jialin Chen, Chuwen Wang, Yiguang Wang, Yuxi Li, Yanan Song, Aiping Jiang, Jiafu Chen, Sumei Chen, Fadi 

作者机构：Minist Agr & Rural Affairs State Key Lab Crop Genet & Germplasm Enhancement & Key Lab Landscaping Nanjing Jiangsu Peoples R China Zhongshan Biol Breeding Lab Key Lab State Forestry & Grassland Adm Biol Orname Nanjing Jiangsu Peoples R China Nanjing Agr Univ Coll Hort Nanjing Jiangsu Peoples R China 

出 版 物：《PLANT CELL AND ENVIRONMENT》 (Plant Cell Environ.)

年 卷 期：2025年第48卷第5期

页      面：3750-3765页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：National Natural Science Foundation of China Hainan Provincial Natural Science Foundation of China China Agriculture Research System JBGS" Project of Seed Industry Revitalization in Jiangsu Province Earmarked fund for Jiangsu Agricultural Industry Technology System [LSM800] Priority Academic Program Development of Jiangsu Higher Education Institutions 32372745 322QN340 CARS-23-A18 JBGS020 

主　　题：anthocyanin biosynthesis chrysanthemum CmBBX28 CmMYB9a light 

摘      要：Light is one of the most important environmental factors that affect plant growth and development. It also stimulates anthocyanin biosynthesis in plants. However, the precise molecular mechanisms through which light regulates anthocyanin biosynthesis, particularly in non-model plant species, remain poorly understood. In this study, we discovered a CmBBX28-CmMYB9a molecular module that is responsive to light and regulates anthocyanin biosynthesis in chrysanthemums. Specifically, CmBBX28 interacts with CmMYB9a, interfering with its binding to the promoters of target genes and reducing the protein abundance of CmMYB9a. This interaction downregulates the transcription of CmMYB9a s downstream anthocyanin-associated genes, CmCHS, CmDFR, and CmUFGT. The expression of CmBBX28 was induced in the dark, and the accumulated CmBBX28 proteins interfered with the activation of CmMYB9a during anthocyanin biosynthesis. Concurrently, darkness also inhibited the expression of CmMYB9a to some extent. In contrast, light significantly induced the expression of CmMYB9a and suppressed the expression of CmBBX28, resulting in increased anthocyanin accumulation in chrysanthemum petals. Our findings reveal the mechanism by which light regulates anthocyanin biosynthesis in chrysanthemum flower petals.