Gas chromatography with simultaneous detection: Ultraviolet spectroscopy, flame ionization, and mass spectrometry

作     者：Gras, Ronda Luong, Jim Haddad, Paul R. Shellie, Robert A. 

作者机构：Dow Chem Canada ULC Highway 15 Ft Saskatchewan AB T8L 2P4 Canada Univ Tasmania ARC Training Ctr Portable Analyt Separat Technol Private Bag 75 Hobart Tas 7001 Australia Univ Tasmania Australian Ctr Res Separat Sci ACROSS Private Bag 75 Hobart Tas 7001 Australia Trajan Sci & Med 7 Argent Pl Ringwood 3134 Australia RMIT Univ Sch Sci Private Bag 2476V Melbourne Vic 3001 Australia 

出 版 物：《JOURNAL OF CHROMATOGRAPHY A》 (J. Chromatogr. A)

年 卷 期：2018年第1563卷

页      面：171-179页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：Australian Research Council's Industrial Transformation Training Centre Scheme [IC140100022] 

主　　题：Gas chromatography Hyphenation Diode array detection Heated DAD cell Mass spectrometry 

摘      要：An effective analytical strategy was developed and implemented to exploit the synergy derived from three different detector classes for gas chromatography, namely ultraviolet spectroscopy, flame ionization, and mass spectrometry for volatile compound analysis. This strategy was achieved by successfully hyphenating a user-selectable multi-wavelength diode array detector featuring a positive temperature coefficient thermistor as an isothermal heater to a gas chromatograph. By exploiting the non-destructive nature of the diode array detector, the effluent from the detector was split to two parallel detectors;namely a quadrupole mass spectrometer and a flame ionization detector. This multi-hyphenated configuration with the use of three detectors is a powerful approach not only for selective detection enhancement but also for improvement in structural elucidation of volatile compounds where fewer fragments can be obtained or for isomeric compound analysis. With the diode array detector capable of generating high resolution gas phase spectra, the information collected provides useful confirmatory information without a total dependence on the chromatographic separation process which is based on retention time. This information-rich approach to chromatography is achieved without incurring extra analytical time, resulting in improvements in compound identification accuracy, analytical productivity, and cost. Chromatographic performance obtained from model compounds was found to be acceptable with a relative standard deviation of the retention times of less than 0.01% RSD, and a repeatability at two levels of concentration of 100 and 1000 ppm (v/v) of less than 5% (n=10). With this configuration, correlation of data between the three detectors was simplified by having near identical retention times for the analytes studied. (C) 2018 Elsevier B.V. All rights reserved.