Synaptic bouton properties are tuned to best fit the prevailing firing pattern

作     者：Knodel, Markus M. Geiger, Romina Ge, Lihao Bucher, Daniel Grillo, Alfio Wittum, Gabriel Schuster, Christoph M. Queisser, Gillian 

作者机构：Heidelberg Univ Bernstein Grp Detailed Modeling Signal Proc Neuro Heidelberg Germany Goethe Univ Frankfurt Heidelberg Germany Goethe Univ Frankfurt Goethe Ctr Sci Comp Dept Simulat & Modeling D-60325 Frankfurt Germany Bernstein Ctr Computat Neurosci Heidelberg Mannhe Heidelberg Germany Heidelberg Univ Dept Neurobiol Interdisciplinary Ctr Neurosci Heidelberg Germany European Mol Biol Lab Dev Unit D-69012 Heidelberg Germany Politecn Torino Dept Math Sci Turin Italy Goethe Univ Frankfurt Goethe Ctr Sci Comp Dept Computat Neurosci D-60325 Frankfurt Germany 

出 版 物：《FRONTIERS IN COMPUTATIONAL NEUROSCIENCE》 (Front. Comput. Neurosci.)

年 卷 期：2014年第8卷第September期

页      面：101页

核心收录：

基　　金：Bernstein group DMSPiN (BMBF) [01GQ0803] German Ministry of Education and Research (BMBF) [01GQ1003A] NeFF Network of Frankfurt University 

主　　题：neuromuscular junction boutons modeling and simulation structure-function relationships morphology firing pattern adaption 

摘      要：The morphology of presynaptic specializations can vary greatly ranging from classical single-release-site boutons in the central nervous system to boutons of various sizes harboring multiple vesicle release sites. Multi-release-site boutons can be found in several neural contexts, for example at the neuromuscular junction (NMJ) of body wall muscles of Drosophila larvae. These NMJs are built by two motor neurons forming two types of glutamatergic multi-release-site boutons with two typical diameters. However, it is unknown why these distinct nerve terminal configurations are used on the same postsynaptic muscle fiber. To systematically dissect the biophysical properties of these boutons we developed a full three-dimensional model of such boutons, their release sites and transmitter-harboring vesicles and analyzed the local vesicle dynamics of various configurations during stimulation. Here we show that the rate of transmission of a bouton is primarily limited by diffusion-based vesicle movements and that the probability of vesicle release and the size of a bouton affect bouton-performance in distinct temporal domains allowing for an optimal transmission of the neural signals at different time scales. A comparison of our in silico simulations with in vivo recordings of the natural motor pattern of both neurons revealed that the bouton properties resemble a well-tuned cooperation of the parameters release probability and bouton size, enabling a reliable transmission of the prevailing firing-pattern at diffusion-limited boutons. Our findings indicate that the prevailing firing-pattern of a neuron may determine the physiological and morphological parameters required for its synaptic terminals.