Large-scale plasmonic microarrays for label-free high-throughput screening

作     者：Chang, Tsung-Yao Huang, Min Yanik, Ahmet Ali Tsai, Hsin-Yu Shi, Peng Aksu, Serap Yanik, Mehmet Fatih Altug, Hatice 

作者机构：MIT Dept Elect Engn & Comp Sci Cambridge MA 02139 USA Boston Univ Dept Elect & Comp Engn Boston MA 02215 USA MIT Dept Biol Engn Cambridge MA 02139 USA 

出 版 物：《LAB ON A CHIP》 (微型实验室：化学与生物学的微型化)

年 卷 期：2011年第11卷第21期

页      面：3596-3602页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：National Science Foundation (NSF) [ECCS-0954790] Office of Naval Research Massachusetts Life Science Center NSF Engineering Research Center on Smart Lighting [EEC-0812056] Boston University Photonics Center Foxconn sponsorship 

主　　题：microarray technology large sizes Scanning systems Microarray Sensors Microscope slide Protein-Protein Interaction 

摘      要：Microarrays allowing simultaneous analysis of thousands of parameters can significantly accelerate screening of large libraries of pharmaceutical compounds and biomolecular interactions. For large-scale studies on diverse biomedical samples, reliable, label-free, and high-content microarrays are needed. In this work, using large-area plasmonic nanohole arrays, we demonstrate for the first time a large-scale label-free microarray technology with over one million sensors on a single microscope slide. A dual-color filter imaging method is introduced to dramatically increase the accuracy, reliability, and signal-to-noise ratio of the sensors in a highly multiplexed manner. We used our technology to quantitatively measure protein-protein interactions. Our platform, which is highly compatible with the current microarray scanning systems can enable a powerful screening technology and facilitate diagnosis and treatment of diseases.