Reducing effects of aberration in 3D fluorescence imaging using wavefront coding with a radially symmetric phase mask

作     者：Patwary, Nurmohammed King, Sharon V. Saavedra, Genaro Preza, Chrysanthe 

作者机构：Univ Memphis Computat Imaging Res Lab Elect & Comp Engn Dept Memphis TN 38152 USA Univ Valencia Imaging & Display Lab 3D Dept Opt E-46100 Burjassot Spain 

出 版 物：《OPTICS EXPRESS》 (Opt. Express)

年 卷 期：2016年第24卷第12期

页      面：12905-12921页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：National Science Foundation (NSF) (DBI) [0844682, 0852847, 1353904] University of Memphis (UoM) Herff Fellowship at UoM Ministerio de Economia y Competitividad, Spain [DPI2015-66458-C2-1-R] Generalitat Valenciana [PROMETEOII/2014/072] Direct For Biological Sciences Div Of Biological Infrastructure [0852847, 1353904] Funding Source: National Science Foundation Div Of Biological Infrastructure Direct For Biological Sciences Funding Source: National Science Foundation 

主　　题：Extended depth of field Image processing Imaging systems Light sheet microscopy Liquid crystal modulators Wavefront encoding 

摘      要：In this work, a wavefront encoded (WFE) imaging system built using a squared cubic phase mask, designed to reduce the sensitivity of the imaging system to spherical aberration, is investigated. The proposed system allows the use of a space-invariant image restoration algorithm, which uses a single PSF, to restore intensity distribution in images suffering aberration, such as sample-induced aberration in thick tissue. This provides a computational advantage over depth-variant image restoration algorithms developed previously to address this aberration. Simulated PSFs of the proposed system are shown to change up to 25% compared to the 0 mu m depth PSF (quantified by the structural similarity index) over a 100 mu m depth range, while the conventional system PSFs change up to 84%. Results from experimental test-sample images show that restoration error is reduced by 29% when the proposed WFE system is used instead of the conventional system over a 30 mu m depth range. (C) 2016 Optical Society of America