250 kHz, 1.5 μm resolution SD-OCT for <i>in</i>-<i>vivo</i> cellular imaging of the human cornea

作     者：Tan, Bingyao Hosseinaee, Zohreh Han, Le Kralj, Olivera Sorbara, Luigina Bizheva, Kostadinka 

作者机构：Univ Waterloo Dept Phys & Astron Waterloo ON N2L 3G1 Canada Singapore Natl Eye Ctr Singapore Eve Res Inst Singapore Singapore Univ Waterloo Dept Syst Design Engn Waterloo ON N2L 3G1 Canada Univ Waterloo Sch Optometry & Vis Sci Waterloo ON N2L 3G1 Canada 

出 版 物：《BIOMEDICAL OPTICS EXPRESS》 (生物医学光学快报)

年 卷 期：2018年第9卷第12期

页      面：6569-6583页

核心收录：

学科分类：0710[理学-生物学] 070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 1009[医学-特种医学] 0702[理学-物理学] 

基　　金：Canadian Institutes of Health Research (CIHR) Natural Sciences and Engineering Research Council of Canada (NSERC) [446387, 312037] University of Waterloo Research Incentive Fund 

主　　题：Full field optical coherence tomography High speed photography Image processing Image processing algorithms Optical imaging Three dimensional imaging 

摘      要：We present the first spectral domain optical coherence tomography (SD-OCT) system that combines an isotropic imaging resolution of similar to 1.5 mu m in biological tissue with a 250 kHz image acquisition rate, for in vivo non-contact, volumetric imaging of the cellular structure of the human cornea. OCT images of the healthy human cornea acquired with this system reveal the cellular structure of the corneal epithelium, cellular debris and mucin clusters in the tear film, the shape, size and spatial distribution of the sub-basal corneal nerves and keratocytes in the corneal stroma, as well as reflections from endothelial nuclei. The corneal images presented here demonstrate the potential clinical value of the new high speed. high resolution OCT system for non-invasive diagnostics and monitoring the treatment of corneal diseases. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement