Molecular screening on a compact disc

作     者：La Clair, JJ Burkart, MD 

作者机构：BIONIC BROS GmbH D-13162 Berlin Germany Univ Calif San Diego Dept Chem & Biochem La Jolla CA 92093 USA 

出 版 物：《ORGANIC & BIOMOLECULAR CHEMISTRY》 (有机与生物分子化学)

年 卷 期：2003年第1卷第18期

页      面：3244-3249页

核心收录：

学科分类：07[理学] 0703[理学-化学] 

主　　题：CTLA4 gene NOD2 gene microarray technology Biomolecules PRINT polycarbonate ink jet printers polymer composite Compact disc players small molecule 

摘      要：A method is described to screen the recognition between small molecule ligands and biomolecules using a conventional compact disc ( CD) player. A procedure was developed to attach ligands to the reading face of a CD by activating the terminus of polycarbonate, a common polymer composite within the reading face of a CD. Terminal residues of the polycarbonate surface 1 were phosphorylated by reaction with ethyl-(N,N)-diisopropylamine- buffered dichloro-(N, N)-diisopropylaminophosphate to yield surface-bound chlorophosphate 2. Ligands containing a primary alcohol were condensed with 2 providing a polycarbonate capped with phosphodiester linked ligands 3 - 6. Displays were generated on the surface of a CD by printing tracks of ligands 3 - 6 on the disc with an inkjet printer. Using this method, discs were created with entire assemblies of ligand molecules distributed into separate blocks. A molecular array was developed by assembling collections of these blocks to correlate with the CDROM-XA formatted data stored within the digital layer of the disc. Regions of the disc containing a given ligand or set of ligands was marked by its spatial position using the tracking and header information. Recognition between surface expressed ligands and biomolecules was screened by an error determination routine.