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作者机构:Univ Sao Paulo Inst Ciencias Biomed Dept Parasitol BR-05508900 Sao Paulo Brazil Inst Butantan Lab Artropodes BR-05503900 Sao Paulo Brazil Univ Sao Paulo Inst Quim Dept Bioquim BR-05508900 Sao Paulo Brazil Univ Sao Paulo Dept Biofis BR-04044020 Sao Paulo Brazil
出 版 物:《JOURNAL OF BIOLOGICAL CHEMISTRY》 (生物化学杂志)
年 卷 期:1999年第274卷第36期
页 面:25330-25334页
核心收录:
学科分类:0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学]
主 题:氨基酸序列 抗菌药 抗感染药/化学 抗感染药/分离和提纯 抗感染药/药理学 真菌/药物作用 革兰氏阳性菌/药物作用 血红蛋白类/化学 血红蛋白类/分离和提纯 血红蛋白类/药理学 分子序列数据 肽碎片/化学 肽碎片/分离和提纯 肽碎片/药理学 蜱/代谢 蜱/微生物学 动物 牛
摘 要:Antifungal and antibacterial activities were detected in the hemolymph and gut contents of the cattle tick, Boophilus microplus. A peptide with antibacterial activity from the tick gut contents was purified to homogeneity by reversed-phase chromatography. The molecular mass of the purified peptide was 3,205.7 Da, measured by matrix-assisted laser desorption/ionization mass spectrometry. The amino acid sequence was obtained by Edman degradation and showed that the peptide was identical to a fragment of the bovine cu-hemoglobin. A synthetic peptide based on the sequence obtained showed characterization data identical to those of the isolated material, confirming its structure. The synthetic peptide was active in micromolar concentrations against Gram-positive bacteria and fungi. These data led us to conclude that the antibacterial activity detected in tick gut contents is the result of enzymatic processing of a host protein, hemoglobin. This activity may be used by ticks as a defense against microorganisms.