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Evaluation and compare of yeast β-glucan and carboxymethylglucan to improve the immunity and gut health of turbot fed diet containing 400 g kg^-1 of soybean meal

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AQUACULTURE REPORTS 2021年 21卷

作者： Gu, Min Pan, Shihui li, Qing Qi, Zezheng Deng, Wanzhen Chen, chuwen Bai, Nan Shandong Univ Marine Coll Weihai 264209 Shandong Peoples R China

In carnivorous fish feed, soybean meal was widely used as the alternative protein source to fish meal, but its negative influence on fish immunity and gut health limits its application. Yeast beta-glucan is a well-documented immunostimulant and prebiotic, and carboxymethylglucan has higher potential bioactivity. The present work was to investigate and compare the effects of yeast beta-glucan and carboxymethylglucan on the immunity and gut health of turbot Scophthalmus maximus (with initial weight of 7.6 g) fed a diet containing 400 g kg(-1) soybean meal. Yeast beta-glucan and carboxymethylglucan was respectively supplemented at the dose of 2 g kg(-1). Enteritis was observed in fish fed the soybean meal diet. Beta-glucan or carboxymethylglucan supplementation significantly increased serum lysozyme activity, and the concentrations of IgM, complement 3 and complement 4. These two additives significantly decreased the infiltration of leukocytes in the lamina propria and submucosa and the expression of inflammatory cytokine of il-8. Compared with beta-glucan, the fish fed carboxymethylglucan showed higher growth performance, feed utilization, lysozyme activity, concentrations of IgM, complement 3 and complement 4 but lower enteritis severity and inflammatory cytokines expression (il-lp, l beta-8, tnf-a and tgf-beta). The structure of intestinal autochthonous bacteria was also altered by dietary yeast beta-glucan and carboxymethylglucan. In conclusion, the present work confirmed the beneficial influence of dietary yeast beta-glucan and carboxymethylglucan on turbots suffering from soy enteropathy. And carboxymethylglucan showed better protective effects than beta-glucan against soybean meal-induced intestinal inflammation on turbot.

关键词： Gut health Yeast beta-glucan Carboxymethylation Soybean meal Turbot

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补肾运脾方促矮小大鼠生长的效用机制研究

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世界中医药 2024年第11期19卷 1619-1624页

作者：张利赵鋆金莺刘建刚杨楚文宋勤丽芦小叶叶青艳上海中医药大学附属曙光医院儿科上海201203 浙江省中医院儿童重症康复科杭州311100 上海市第七人民医院儿科上海200137

目的:观察补肾运脾方对矮小大鼠生长的影响,探讨其作用机制。方法:将雌性30只、雄性15只大鼠合笼过夜,雌鼠受孕后按随机数字表随机分为低蛋白饲料组与标准饲料组,所有孕鼠分娩后采用标准饲料喂养。仔鼠2周龄去除雌仔鼠,3周龄离乳。标准... 详细信息

目的:观察补肾运脾方对矮小大鼠生长的影响,探讨其作用机制。方法:将雌性30只、雄性15只大鼠合笼过夜,雌鼠受孕后按随机数字表随机分为低蛋白饲料组与标准饲料组,所有孕鼠分娩后采用标准饲料喂养。仔鼠2周龄去除雌仔鼠,3周龄离乳。标准饲料组孕鼠分娩的新生仔鼠中按随机数字表随机方法选取10只雄鼠仔鼠标记为正常组,选矮小模型仔鼠50只分为模型组、西药组、低剂量组、中剂量组、高剂量组,每组10只。正常组、模型组给予无菌生理盐水灌胃,1 mL/100 g;低、中、高剂量组分别给予补肾运脾方药液0.76 g/mL、1.52 g/mL、3.04 g/mL灌胃,1 mL/100 g;西药组给予重组人生长激素注射液腹部皮下注射(0.84 IU/kg)。每天上午8:00给药,1次/d,连续3周。对比各组身长、体质量、骨密度(BMD)、骨矿盐(BMC)及血清Ⅰ型前胶原羧基端肽(PICP)、Ⅰ型胶原羧基吡啶交联肽(ICTP)、生长激素(GH)、胰岛素样生长因子-1(IGF-1)。结果:各组大鼠治疗后身长、体质量高于治疗前(P<0.05),低剂量组、高剂量组BMD高于模型组(P<0.05),正常组、西药组、中剂量组、高剂量组血清PICP含量低于模型组(P<0.05),中药3组血清PICP含量大于正常组(P<0.05),正常组、中药3组、西药组血清ICTP含量小于模型组(P<0.01),中药3组血清ICTP含量大于正常组(P<0.05);正常组、中药3组、西药组血清GH含量小于模型组(P<0.01),低剂量组、中剂量组血清GH含量大于西药组(P<0.05),低剂量组血清GH含量大于正常组(P<0.05);低剂量组、中剂量组血清IGF-1水平小于西药组(P<0.05)。结论:补肾运脾方可促进矮小大鼠身长、体质量增长,促进骨代谢,增加BMD,其效用机制为通过促进血清GH利用、提高血清IGF-1水平发挥促生长作用。

关键词：补肾运脾方中药矮小大鼠骨密度生长激素胰岛素样生长因子-1 Ⅰ型前胶原羧基端肽

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水包油型灵芝多糖纳米乳佐剂的制备及乳化方法探究

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畜牧与兽医 2023年第11期55卷 65-71页

作者：王楚文李玉林石盼盼左蕾闫生辉王云龙郑州大学生命科学学院河南郑州450000 河南省生物工程技术研究中心河南郑州450000 郑州职业技术学院生命健康学院河南郑州450000

研发一种水包油型灵芝多糖纳米乳(GLP-NE)佐剂,初步应用于非洲猪瘟病毒P72重组抗原制剂研究,以提升P72免疫制剂稳定性和有效性。本研究利用伪三元相图法确定最佳组方,采用剪切搅拌、超声2种乳化方法筛选制备GLP-P72纳米乳的工艺参数,并... 详细信息

研发一种水包油型灵芝多糖纳米乳(GLP-NE)佐剂,初步应用于非洲猪瘟病毒P72重组抗原制剂研究,以提升P72免疫制剂稳定性和有效性。本研究利用伪三元相图法确定最佳组方,采用剪切搅拌、超声2种乳化方法筛选制备GLP-P72纳米乳的工艺参数,并检验其安全性、物理性状及免疫效果。结果表明,灵芝多糖纳米乳佐剂为水包油型且符合纳米乳佐剂要求,粒径为(167.4±0.1)nm,黏度为4.2 s·(0.4 mL)^(-1),1 500 r·min^(-1)剪切搅拌20 min可制备出较为稳定的纳米乳。该佐剂经济有效,乳化方法简单稳定,可使GLP-P72纳米乳具有较好的稳定性和免疫效果,并对中药成分纳米乳佐剂的研发和制备提供借鉴。

关键词：纳米乳佐剂灵芝多糖乳化方法

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不同鸡种胚胎腿肌差异表达mRNA和lncRNA的筛选及其竞争性调控网络的构建

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畜牧兽医学报 2022年第12期53卷 4207-4220页

作者：李洁赵瑞鹏陈楚雯杨朝武吴锦波李志雄西南民族大学青藏高原动物遗传资源保护与利用教育部重点实验室成都610041 西南民族大学畜牧兽医学院成都610041 西南民族大学青藏高原研究院成都610041 四川省畜牧科学研究院成都610066 阿坝藏族羌族自治州畜牧科学技术研究所阿坝624402

旨在筛选藏鸡和大恒肉鸡胚胎期骨骼肌差异表达的mRNA和lncRNA,并构建lncRNA-miRNA-mRNA竞争性调控网络,为进一步探讨两个鸡品种骨骼肌生长发育速度的差异机制奠定基础。随机选取已孵化18 d的藏鸡和大恒肉鸡胚胎各3个,采集胚胎腿肌组织... 详细信息

旨在筛选藏鸡和大恒肉鸡胚胎期骨骼肌差异表达的mRNA和lncRNA,并构建lncRNA-miRNA-mRNA竞争性调控网络,为进一步探讨两个鸡品种骨骼肌生长发育速度的差异机制奠定基础。随机选取已孵化18 d的藏鸡和大恒肉鸡胚胎各3个,采集胚胎腿肌组织进行转录组测序。筛选两个品种中差异表达的mRNAs和lncRNAs,对差异表达基因进行GO和KEGG富集分析,并对lncRNA的靶标miRNA以及靶向mRNA的miRNA进行预测,筛选与肌肉发育相关的mRNA和lncRNA构建lncRNA-miRNA-mRNA竞争性调控网络,最后利用实时荧光定量PCR(qRT-PCR)对测序结果进行验证。结果显示,共有106个mRNAs在两个品种中差异表达,其中上调表达mRNAs 48个,下调表达mRNAs 58个。差异表达lncRNAs共有28个,其中10个上调,18个下调。差异表达mRNA的GO富集结果显示,骨骼肌细胞分化条目被显著富集,KEGG富集分析中脂质代谢通路被显著富集。lncRNA靶基因的KEGG富集结果显示,在10个显著富集通路中,有基因显著富集于类固醇生物合成和脂肪酸生物合成等与脂质代谢相关的信号通路。筛选与骨骼肌发育相关的差异表达mRNAs和lncRNAs构建了骨骼肌发育相关的lncRNA-miRNA-mRNA调控网络,qRT-PCR结果显示其表达趋势与转录组测序结果一致,证实测序数据准确可靠。本研究筛选出藏鸡和大恒肉鸡胚胎期差异表达的mRNA和lncRNA,并构建了骨骼肌发育相关的lncRNA-miRNA-mRNA调控网络,为揭示不同鸡品种中骨骼肌生长发育的差异性提供理论依据。

关键词：藏鸡大恒肉鸡转录组测序肌肉发育调控网络

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TrustGWAS: A full-process workflow for encrypted GWAS using multi-key homomorphic encryption and pseudorandom number perturbation

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CELL SYSTEMS 2022年第9期13卷 752-+页

作者： Yang, Meng Zhang, chuwen Wang, Xiaoji liu, Xingmin li, Shisen Huang, Jianye Feng, Zhimin Sun, Xiaohui Chen, Fang Yang, Shuang Ni, Ming li, lin Cao, Yanan Mu, Feng BGI Shenzhen MGI Shenzhen 518083 Peoples R China Univ Copenhagen Dept Biol DK-2200 Copenhagen Denmark Shanghai Jiao Tong Univ Ruijin HospRes Unit Clin & Basic Res Metab Dis Chinese Acad Med SciSch MedNatl Clin Res Ctr Me Dept Endocrine & Metab DisShanghai Inst Endocrin Shanghai 200025 Peoples R China SJTU Inst Translat Med Natl Ctr Translat Med Shanghai Shanghai 200240 Peoples R China BGI Shenzhen Shenzhen 518083 Peoples R China BGI Shenzen MGI QingDao Qingdao 266000 Peoples R China SJTU BGI Innovat Res Ctr Shanghai Peoples R China

The statistical power of genome-wide association studies (GWASs) is affected by the effective sample size. However, the privacy and security concerns associated with individual-level genotype data pose great challenges for cross-institutional cooperation. The full-process cryptographic solutions are in demand but have not been covered, especially the essential principal-component analysis (PCA). Here, we present TrustGWAS, a complete solution for secure, large-scale GWAS, recapitulating gold standard results against PliNK without compromising privacy and supporting basic PliNK steps including quality control, linkage disequilibrium pruning, PCA, chi-square test, Cochran-Armitage trend test, covariate-supported logistic regression and linear regression, and their sequential combinations. TrustGWAS leverages pseudorandom number perturbations for PCA and multiparty scheme of multi-key homomorphic encryption for all other modules. TrustGWAS can evaluate 100,000 individuals with 1 million variants and complete QC-LD-PCAregression workflow within 50 h. We further successfully discover gene loci associated with fasting blood glucose, consistent with the findings of the ChinaMAP project.

关键词： TrustGWAS multi-key homomorphic encryption GWAS CKKS privacy-preserving computation pseudorandom number genome privacy

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Analysis of the cutting surface striation shape 7th

Analysis of the cutting surface striation shape

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7th International Conference on Material Science and Engineering Technology, ICMSET 2018

作者： li, liucan Xu, Qiwen Guo, chuwen School of Electrical and Power Engineering China University of Mining and Technology Xuzhou China

ISBN: (纸本)9783035715392

In the process of abrasive water jet (AWJ) cutting, there will produce a series of striation shape on the cutting surface of target. In this paper, we define such striation as cutting striation shape. The most research of this paper is the force situation and movement trajectory of water jet in the process of cutting. It is deduced by theory that the cutting surface striation shape is circular arc and find that the radius of circular arc is related to cutting velocity, cutting pressure and the property of cutting material. In the experiment, stainless steel 304 and aluminum 6061 were cut by AWJ and the result shows that the shape of the surface striation is arc-shaped in both material surface. At different cutting pressure, for a same material, KR is a constant value which is only related to the property of cutting material. © 2019 Trans Tech Publications, Switzerland.

关键词： Aluminum alloys

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针刺治疗轻度认知功能障碍的机制和临床研究进展

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中医药信息 2024年第4期41卷 70-74页

作者：佟欣秦鸿宇曹丹娜冯楚文李晓陵黑龙江中医药大学黑龙江哈尔滨150040 黑龙江中医药大学附属第一医院黑龙江哈尔滨150040

轻度认知功能障碍(MCI)是正常老年化和老年期阿尔茨海默病之间的过渡阶段。据统计,10%~15%MCI患者会转变为AD,转化率是正常老龄化的10倍。针刺作为中医学的重要组成部分,疗效和安全性已经过国内大量临床验证,其具有多靶点、多途径和多... 详细信息

轻度认知功能障碍(MCI)是正常老年化和老年期阿尔茨海默病之间的过渡阶段。据统计,10%~15%MCI患者会转变为AD,转化率是正常老龄化的10倍。针刺作为中医学的重要组成部分,疗效和安全性已经过国内大量临床验证,其具有多靶点、多途径和多层面的作用特点,安全、有效,在临床中广泛用于治疗MCI。笔者对针刺治疗MCI的机制和临床相关研究文献进行总结,发现针刺可以抑制Aβ沉积、Tau蛋白的过度磷酸化、炎症反应、氧化应激,调节胆碱能系统,发挥治疗作用;在临床治疗中,针药结合治疗MCI的疗效更好,具有良好的协同作用。

关键词：针刺轻度认知功能障碍综述

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Oral Delivery of a Nanocrystal Formulation of Schisantherin A with Improved Bioavailability and Brain Delivery for the Treatment of Parkinson's Disease

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MOLECULAR PHARMACEUTICS 2016年第11期13卷 3864-3875页

作者： Chen, Tongkai li, chuwen li, Ye Yi, Xiang Lee, Simon Ming-Yuen Zheng, Ying Univ Macau State Key Lab Qual Res Chinese Med Inst Chinese Med Sci Macau Peoples R China Univ North Carolina Chapel Hill UNC Eshelman Sch Pharm Div Mol Pharmaceut Chapel Hill NC 27599 USA

Schisantherin A (SA) is a promising anti-Parkinsonism Chinese herbal medicine but with poor water solubility and challenges to be delivered to the brain. We formulated SA as nanocrystals (SA-NC), aiming to improve its solubility and pharmacokinetic profile and thus provide a potential therapeutic agent for the treatment of Parkinson's disease (PD). The rod-shaped SA-NC had a particle size of, similar to 160 nm with 33.3% drug loading, and the nanocrystals exhibited a fast dissolution rate in vitro. The intact drug nanocrystals could be internalized into Madin-Darby canine kidney (MDCK) cells, which were followed by rapid intracellular release, and most of the drug was transported to the basolateral side in its soluble form. Following oral administration of the SA-NC or an SA suspension, the accumulated concentration of the SA-NC in the plasma and brain was considerably higher than that observed for the SA suspension, but the drug targeting efficiency was similar. The SA-NC significantly reversed the 1methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic (DA) neuronal loss and locomotion deficiency in zebrafish, as well as the 1-methyl-4-phenylpyridinium ion (MPP+)-induced damage of neuronal cell culture model. Further Western blot analysis demonstrated that the stronger neuroprotective effect of SA-NC may be partially mediated by the activation of the protein kinase B (Akt)/glycogen synthase kinase-3 beta (Gsk3 beta) pathway. Taken together, these data provide solid evidence that the nanocrystal formulation has the potential to improve the bioavailability and brain concentration of this Biopharmaceutics Classification System (BCS) class II compound, SA, for the treatment of PD.

关键词： nanocrystals dissolution oral bioavailability brain delivery Parkinson's disease (PD)

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Biomimicry-inspired zwitterionic polyurethane used for vascular implants showing water-induced stiffening and preventing intimal hyperplasia in stent

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Biomaterials 2025年 322卷 123394-123394页

作者： Yang, Ruibo Chen, chuwen liu, Wenkai Wang, Ao Jiang, Pengjun li, Zhen Luo, Feng li, Jiehua Tan, Hong College of Polymer Science and Engineering National Key Laboratory of Advanced Polymer Materials Med-X Center for Materials Sichuan University Chengdu610065 China Division of Vascular Surgery Department of General Surgery West China Hospital Sichuan University Chengdu610061 China Department of Laboratory Medicine Med+X Center for Manufacturing West China Hospital Sichuan University Sichuan Chengdu610041 China

Polymeric vascular implants with ideal mechanical properties and biocompatibility are essential for dilating blood vessels and reducing the risk of secondary implant diseases. However, traditional polymer materials are still limited for vascular stents by diminished radial support post-expansion and inadequate surface modification techniques. Herein, we synthesized zwitterionic polyurethanes (ZPUs) featuring hydrophilic side chains derived from betaine sulfonate and full-hard main chains. These ZPUs demonstrate a remarkable increase in modulus during shape recovery in 37 °C warm water, ensuring that the stent remains soft during implantation for easy delivery, but becomes stiff once positioned at the lesion site to provide adequate radial support. The distinctive architecture promotes the migration of hydrophilic side chains to the surface upon hydration, establishing a "core-shell structure" with a hard interior and a highly hydrophilic surface that enhances antithrombotic properties, mitigates inflammation, and curbs intimal hyperplasia. Consequently, ZPUE20 stent showed significantly better blood flow patency than traditional PLA stent in carotid artery implantation for at least 3 months, ensuring the long-term biological safety of implantation. Compared to surface modification of bare stents, ZPU stents avoid the complex and unstable surface modifications. All in all, ZPUs represent a promising material for vascular implants, markedly improving both mechanical performance and biocompatibility. © 2025

关键词： Stents

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Ultra-Fast Bloom Filters using SIMD Techniques

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IEEE TRANSACTIONS ON PARALLEL AND DISTRIBUTED SYSTEMS 2019年第4期30卷 953-964页

作者： Lu, Jianyuan Wan, Ying li, Yang Zhang, chuwen Dai, Huichen Wang, Yi Zhang, Gong liu, Bin Tsinghua Univ Beijing 100084 Peoples R China Alibaba Cloud Beijing 100102 Peoples R China Tsinghua Univ Dept Comp Sci & Technol Beijing 100084 Peoples R China Huawei Future Network Theory Lab Hong Kong Peoples R China

The network link speed is growing at an ever-increasing rate, which requires all network functions on routers/switches to keep pace. Bloom filter is a widely-used membership check data structure in networking applications. Correspondingly, it also faces the urgent demand of improving the performance in membership check speed. To this end, this paper proposes a new Bloom filter variant called Ultra-Fast Bloom Filters (UFBF), by leveraging the Single Instruction Multiple Data (SIMD) techniques. We make three improvements for UFBF to accelerate the membership check speed. First, we develop a novel hash computation algorithm which can compute multiple hash functions in parallel with the use of SIMD instructions. Second, we elaborate a Bloom filter's bit-test process from sequential to parallel, enabling more bit-tests per unit time. Third, we improve the cache efficiency of membership check by encoding an element's information to a small block so that it can fit into a cache-line. We further generalize UFBF, called c-UFBF, to make UFBF supporting large number of hash functions. Both theoretical analysis and extensive evaluations show that the UFBF greatly outperforms the state-of-the-art Bloom filter variants on membership check speed.

关键词： Bloom filter SIMD parallel techniques

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