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Single-cell RNA transcriptome uncovers distinct developmental trajectories in the embryonic skeletal muscle of Daheng broiler and Tibetan chicken

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BMC GENOMICS 2025年第1期26卷 1-18页

作者： li, Jie Yang, Dongmei Chen, chuwen Wang, Jiayan Wang, Zi Yang, Chaowu Yu, Chunlin li, Zhixiong Southwest Minzu Univ Key Lab Qinghai Tibetan Plateau Anim Genet Resourc Minist Educ Chengdu 610041 Peoples R China Southwest Minzu Univ Key Lab Anim Sci Natl Ethn Affairs Commiss China Chengdu 610041 Peoples R China Southwest Minzu Univ Inst Qinghai Tibetan Plateau Chengdu 610041 Peoples R China Sichuan Anim Sci Acad Chengdu 610066 Peoples R China

Different chicken breeds exhibit distinct muscle phenotypes resulting from selective breeding, but little is known about the molecular mechanisms responsible for this phenotypic difference. Skeletal muscle is composed of a large number of heterogeneous cell populations. Differences in differentiation and interaction of cell populations play a key role in the difference of skeletal muscle phenotype. In the current study, we performed a single-cell RNA sequencing (scRNA-seq) on the leg muscle of Daheng broiler (DH, cultivated breed) and Tibetan chicken (TC, native breed) at embryonic (E) 10, E14 and E18. A comprehensive cell atlas of embryonic chicken skeletal muscle, consisting of 29,579 high-quality cells representing 6 distinct cell types was built. The differentiation trajectory of Myoblasts and fibro-adipogenic progenitors (FAPs) was constructed through pseudotemporal trajectory analysis. Our results revealed the different developmental trajectories and dynamic gene expression profiles in 3 subtypes of myoblasts and 5 FAPs subtypes of the two chicken breeds. Tibetan chicken showed earlier embryonic myogenesis and less myoblasts compared with Daheng broiler. By comparing the switch status and switch time of genes in the two breeds, SNRPG,SNRPE,EIF4EBP1 and HSP90AB1 were considered as potentially critical genes for embryonic myogenesis, and the genes MYOG,MYBPH,APOA1, and MGP played dominant roles in the embryonic adipogenesis. Intercellular interaction networks showed that strong and complex intercellular communication was contained during embryonic skeletal muscle growth and development. These findings revealed the differences of molecular mechanisms in the skeletal muscle development between TC and DH chickens. Our data provide a better understanding of skeletal muscle developmental differences between cultivated and native breeds and valuable information for genetic breeding of chicken.

关键词： ScRNA-seq Transcriptional dynamics Myoblasts Fibro-adipogenic progenitor Chicken breeds

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Neuroprotective Effects of BHDPC, a Novel Neuroprotectant, on Experimental Stroke by Modulating Microglia Polarization

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ACS CHEMICAL NEUROSCIENCE 2019年第5期10卷 2434-2449页

作者： li, chuwen Bian, Yaqi Feng, Yu Tang, Fan Wang, liang Hoi, Maggie Pui Man Ma, Dan Zhao, Chao Lee, Simon Ming Yuen Guangzhou Med Univ Sch Pharmaceut Sci Key Lab Mol Target & Clin Pharmacol Guangzhou 510182 Guangdong Peoples R China Univ Macau Inst Chinese Med Sci State Key Lab Qual Res Chinese Med Taipa 999078 Macau Peoples R China Univ Cambridge Dept Clin Neurosci Wellcome Trust MRC Cambridge Stem Cell Inst Cambridge CB2 0AH England

This study mainly investigated the therapeutic effects of BHDPC on ischemic stroke and its underlying mechanisms. In vivo, the transient middle cerebral artery occlusion (MCAO) was used to induce ischemic model. In vitro, oxygen and glucose deprivation/reperfusion (OGD/R)-induced ischemic stroke in BV-2 microglia and primary neurons, and bEnd.3 mouse cerebral microvascular endothelial cells (ECs) were also used. First, we found that BHDPC exerts considerable neuroprotection against MCAO-induced ischemic injury to mice via alleviating neurological deficits and brain infarcts, inhibiting neuronal cell loss and apoptosis, and attenuating blood brain barrier disruption and tight junction protein changes. Next, we observed that BHDPC significantly reduced microglial M1 activation but enhanced M2 polarization in MCAO-induced ischemic brain. Further experiments in vitro indicated that BHDPC suppressed microglial activation but promoted M2 microglial polarization in OGD/R-induced BV-2 microglia. In addition, conditioned medium (CM) experiments showed that CM from BHDPC-treated BV-2 microglia provided protections against OGD/R-induced ischemic damage in primary neurons and bEnd.3 ECs. Moreover, we found that BHDPC actions on microglial inflammation were associated with the inactivation of NF-kappa B signaling. Interestingly, we also found that BHDPC enhanced phosphorylation of protein kinase A (PKA) and cAMP-response element-binding protein (CREB). The pharmacological inhibition or gene knockdown of PKA/CREB signaling diminished BHDPC-promoted microglial M2 polarization. In summary, BHDPC conferred neuroprotection against ischemic injury in experimental stroke models. Modulating microglial activation and polarization contributes to BHDPC-mediated neuroprotective actions, which in part were mediated by nuclear factor kappa B and PKA/CREB signaling pathway.

关键词： BHDPC ischemic stroke microglia NF-kappa B CREB PKA

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The detection and utilization of volatile metabolomics in Klebsiella pneumoniae by gas chromatography-ion mobility spectrometry

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SCIENTIFIC REPORTS 2024年第1期14卷 1-16页

作者： li, Fuxing Gu, Shumin Zhao, chuwen Zheng, Yunwei Zhu, Junqi Hu, Longhua Hang, Yaping Nanchang Univ Affiliated Hosp 2 Jiangxi Med Coll Jiangxi Prov Key Lab Immunol & InflammatJiangxi P Mingde Rd 1 Nanchang 330006 Jiangxi Peoples R China Nanchang Univ Sch Publ Hlth Nanchang Jiangxi Peoples R China

This research aimed to analyze the volatile compounds emitted during the proliferation of Klebsiella pneumoniae (K. pneumoniae) in the laboratory setting using gas chromatography-ion mobility spectrometry (GC-IMS) and to investigate the potential of volatile metabolomics for detecting carbapenemase-producing strains of K. pneumoniae. The volatile metabolomics of K. pneumoniae were comprehensively analyzed using GC-IMS in tryptic soy broth (TSB) as the culture medium. Afterward, the growth stabilization period (T2) served as the primary time point for analysis, with the introduction of imipenem and carbapenemase inhibitors (avibactam sodium or EDTA) during the exponential growth phase (T0) to further investigate alterations in volatile molecules associated with K. pneumoniae. Standard strains were utilized as references, while clinical strains were employed for validation purposes. At T2, a total of 22 volatile organic compounds (VOCs) associated with K. pneumoniae were identified (3 VOCs found in both monomer and dimer forms). Significant differences in VOCs were observed between carbapenemase-negative and carbapenemase-positive strains, both standard and clinical, following the introduction of imipenem. Furthermore, the addition of avibactam sodium led to distinct changes in the VOC content of strains producing class A carbapenemase, while the addition of EDTA resulted in specific alterations in the volatile metabolic profiles of strains producing class B carbapenemase. GC-IMS demonstrated significant promise for analyzing bacterial volatile metabolomics, and its application in evaluating the volatolomics of K. pneumoniae may facilitate the timely detection of carbapenemase-producing strains.

关键词： Gas chromatography-ion mobility spectrometry (GC-IMS) Volatile metabolomic Klebsiella pneumoniae Carbapenemase Imipenem (IPM)

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Ultrasound Irradiation-Induced Superoxide Anion Radical Mediates the Reduction of Tetravalent Platinum Prodrug for Antitumor Therapy

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CCS Chemistry 2025年第4期7卷 1142-1156页

作者： Chaoying Kong Yanran li Yanjun Xu chuwen Luo Na Shen Zhaohui Tang Xuesi Chen Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022 School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026

The development of tetravalent platinum[Pt(IV)]prodrugs with controllable activation properties represents a crucial strategy for the mitigation of the severe side effects associated with bivalent platinum[Pt(Ⅱ)]drugs,which are used in over 50%of all chemotherapeutics ***(US)irradiation is an ideal method for prodrug activation given its noninvasive nature and efficient tissue penetration *** study shows that US irradiation alone could directly convert Pt(IV)prodrugs to Pt(Ⅱ)drugs in an aqueous solution,with a significant increase in the reduction rate in the presence of the sonosensitizer riboflavin(Rf;also known as vitamin B2).Rf accelerated the reduction rate by facilitating the production of the superoxide anion radical(•O_(2)^(−)).Subsequently,Rf and tetravalent cisplatin[cisPt(IV)]prodrug were covalently co-loaded into poly(L-glutamic acid)to create a US catalysis-activated nano prodrug[P-Rf/cisPt(IV)]for antitumor ***,even under low-intensity US irradiation(1.5 W/cm^(2)),P-Rf/cisPt(IV)was effectively converted to cisPt(Ⅱ)in mice,resulting in significant antitumor *** findings will facilitate the advancement of US irradiation-activated Pt(IV)prodrugs for use in chemotherapeutics.

关键词： ultrasound activation ultrasound chemistry platinum drug prodrug superoxide anion radical

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GC-IMS facilitates identification of carbapenem-resistant Klebsiella pneumoniae in simulated blood cultures

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AMB EXPRESS 2024年第1期14卷 40页

作者： li, Fuxing Zheng, Yunwei Zhao, chuwen Zhu, Junqi Hang, Yaping Fang, Youling Hu, Longhua Nanchang Univ Affiliated Hosp 2 Jiangxi Med Coll Nanchang Jiangxi Peoples R China Nanchang Univ Dept Jiangxi Prov Key Lab Med Affiliated Hosp 2 Clin Lab Mingde Rd 1 Nanchang 330006 Jiangxi Peoples R China Nanchang Univ Sch Publ Hlth Nanchang Jiangxi Peoples R China

This study aimed to identify carbapenem-resistant Klebsiella pneumoniae (CRKP) based on changes in levels of its volatile organic compounds (VOCs) in simulated blood cultures (BCs) using the gas chromatography-ion mobility spectrometry (GC-IMS) technique. A comprehensive analysis of volatile metabolites produced by Klebsiella pneumoniae (K. pneumoniae) in BC bottles was conducted using GC-IMS. Subsequently, the released VOCs were analyzed to examine differences in VOC release between CRKP and carbapenem-susceptible Klebsiella pneumoniae (CSKP). A total of 54 VOCs were detected, of which 18 (6 VOCs found in both monomer and dimer forms) were successfully identified. The VOCs produced by K. pneumoniae in BC bottles (BacT/ALERT (R) SA) were primarily composed of organic acids, alcohols, esters, and ketones. The content of certain VOCs was significantly different between CRKP and CSKP after the addition of imipenem (IPM). Moreover, the inclusion of carbapenemase inhibitors facilitated the identification of carbapenemase-producing K. pneumoniae based on the variations in VOCs. This study demonstrates the utility of GC-IMS technology in identifying CRKP, and reveals that changes in VOCs are closely related to the growth and metabolism of K. pneumoniae, indicating that they can be leveraged to promote early identification of CRKP bacteremia. However, further in-depth studies and experiments are needed to validate our findings.

关键词： Carbapenem-resistant Klebsiella pneumoniae (CRKP) Gas chromatography-ion mobility spectrometry (GC-IMS) Volatile organic compounds (VOCs) Imipenem (IPM) Carbapenemase (CBPM)

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Rational design and synthesis of l-carvone-derived 4-methyl-1,2,4-triazole-thioether/nanochitosan complexes as potent nanopesticides for sustainable and efficient herbicidal application

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ENVIRONMENTAL SCIENCE-NANO 2023年第12期10卷 3317-3328页

作者： li, Baoyu Duan, Wengui lin, Guishan Cui, Yucheng Wen, Rongzhu liu, chuwen Xie, Yulu Guangxi Univ Sch Chem & Chem Engn 100 Daxue Dong Rd Nanning 530004 Guangxi Peoples R China Guangxi Univ Coll & Univ Key Lab Appl Chem Technol & Resource D 100 Daxue Dong Rd Nanning 530004 Guangxi Peoples R China

Natural product-based nanopesticide is an effective and sustainable approach for solving the environmental issues caused by the application of current commercial herbicides. In this study, 21 novel l-carvone-derived 4-methyl-1,2,4-triazole-thioether derivatives 5a-5u were rationally designed by transketolase-based in silicon virtual screening, synthesized, and structurally characterized using FT-IR spectroscopy, H-1/C-13 NMR spectroscopy, and HRMS technique. Besides, the herbicidal activity of the target compounds against Brassica campestris and Echinochloa crusgalli L. was evaluated, and the bioassay results revealed that compound 5a with the most significant herbicidal and TK-inhibition activities could serve as a potential TK inhibitor and could serve as a leading compound for further study of green herbicides. Subsequently, the molecular skeletons of chitosan and l-carvone were incorporated for developing high-efficiency nanopesticide carriers, and thus samples 7a-7d were synthesized by N-alkylation of chitosan and characterized using various methods. Compared with unmodified chitosan, l-carvone-derived nanochitosan carrier 7b with the micro-morphologies of nubby lumps and porous surfaces exhibited higher dispersibility and similar thermostability. Finally, l-carvone-derived 4-methyl-1,2,4-triazole-thioether/nanochitosan complexes 7b/5a, 7c/5a, and 7d/5a with better 5a-loading capacities and multi-stage sustained releasing performances were constructed, and the herbicidal activities of 7b/5a and 7d/5a against Brassica campestris were evaluated in the petri dish and greenhouse. It was found that 7b/5a and 7d/5a exhibited significant herbicidal activities. Among them, 7b/5a had great potential as a green and eco-friendly nanopesticide for weed control.

关键词： Herbicides

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Influence of structural parameters of self-excited nozzle on oscillation effect of pulse jet

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JOURNAL OF THE BRAZIliAN SOCIETY OF MECHANICAL SCIENCES AND ENGINEERING 2023年第4期45卷 1-14页

作者： li, Hongsheng liu, Songyong Jiang, Hongxiang Ji, Huifu Zhang, Deyi Sun, Dunkai Guo, chuwen China Univ Min & Technol Sch Mechatron Engn Xuzhou 221116 Peoples R China China Univ Min & Technol Jiangsu Prov & Educ Minist Cosponsored Collaborat Xuzhou 221008 Peoples R China Henan Agr Univ Coll Tobacco Sci Zhengzhou 450008 Peoples R China Suzhou Univ Sci & Technol Sch Mech Engn Suzhou 215009 Peoples R China

To design a self-excited oscillation pulse jet (SOPJ) nozzle with excellent performance and improve the rock breaking efficiency of hard rock roadway, the nonlinear mathematical model of SOPJ is firstly established, and the relationship between jet pressure versus time and pressure gradient with different control parameters beta of self-excited oscillation system is studied. In addition, the internal and external flow fields with different system pressure p, cavity length L-c, cavity diameter D-c and cone angle alpha(c) are studied by numerical simulation to obtain its influence on the oscillation effect of the SOPJ. The results show that the average velocity of SOPJ and continuous jet all shows a power function, but the velocity of SOPJ is lower than that of continuous jet due to the internal energy consumption. The average velocity and variance of the SOPJ are considered as the evaluation criteria for the oscillation effect, the pulse jet presents better oscillation effect with the cavity length, and the cavity diameter and the section cone angle are selected as 80 mm, 30 mm and 160 degrees, respectively, which can provide the basis for the design of self-excited oscillation nozzle and application of pulse jet technology in hard rock roadway driving.

关键词： Self-excited nozzle Pulse jet Nonlinear mathematical model Average velocity Oscillation effect

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Tetramethylpyrazine Analogue T-006 Promotes the Clearance of Alpha-synuclein by Enhancing Proteasome Activity in Parkinson's Disease Models

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NEUROTHERAPEUTICS 2019年第4期16卷 1225-1236页

作者： Zhou, Hefeng Shao, Min Guo, Baojian li, chuwen Lu, Yucong Yang, Xuanjun Shengnanli li, Haitao Zhu, Qi Zhong, Hanbing Wang, Yuqiang Zhang, Zaijun Lu, Jiahong Lee, Simon Ming-Yuen Zunyi Med Univ Dept Bioengn Zhuhai Campus Zhuhai Peoples R China Univ Macau State Key Lab Qual Res Chinese Med Macau Peoples R China Univ Macau Inst Chinese Med Sci Macau Peoples R China Jinan Univ Coll Pharm Inst New Drug Res Guangzhou Peoples R China Guangzhou Med Univ Sch Pharmaceut Sci Key Lab Mol Target & Clin Pharmacol Guangzhou Peoples R China South Univ Sci & Technol Dept Biol Shenzhen Peoples R China

Parkinson's disease (PD) is the second most common neurodegenerative disorder worldwide and is characterized in part by the degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc). The main pathological hallmark of PD is the intraneuronal accumulation of misfolded alpha-synuclein (alpha-syn) aggregates. Mutations in the SNCA gene (encoding alpha-syn) and variations in its copy number are associated with some forms of familial PD. In the present study, T-006, a new tetramethylpyrazine (TMP) derivative with recently reported anti-Alzheimer activity, is shown to significantly promote alpha-syn degradation in a cellular PD model. Moreover, we illustrate that T-006 inhibits the accumulation of both Triton-soluble and -insoluble forms of alpha-syn and protects against alpha-syn-induced neurotoxicity in A53T-alpha-syn transgenic mice. The mechanism of action of T-006 was verified by evaluation of a potential protein degradation pathway. We found that T-006 promotes alpha-syn degradation in a proteasome-dependent and autophagy-independent manner. We further confirmed that T-006 enhances proteasome activity by upregulating 20S proteasome subunit beta 5i (LMP7) protein expression. A functional study revealed that T-006 activates the PKA/Akt/mTOR/p70S6K pathway to trigger LMP7 expression and enhance chymotrypsin-like proteasomal activity. These findings indicate that T-006 is a potent proteasome activator and a potential therapeutic agent for the prevention and treatment of PD and related diseases.

关键词： alpha-synuclein Degradation LMP7 Proteasome activity Parkinson's disease

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CmBBX28-CmMYB9a Module Regulates Petal Anthocyanin Accumulation in Response to light in Chrysanthemum

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PLANT CELL AND ENVIRONMENT 2025年第5期48卷 3750-3765页

作者： Zhou, li-Jie Peng, Jialin Chen, chuwen Wang, Yiguang Wang, Yuxi li, Yanan Song, Aiping Jiang, Jiafu Chen, Sumei Chen, Fadi Minist Agr & Rural Affairs State Key Lab Crop Genet & Germplasm Enhancement & Key Lab Landscaping Nanjing Jiangsu Peoples R China Zhongshan Biol Breeding Lab Key Lab State Forestry & Grassland Adm Biol Orname Nanjing Jiangsu Peoples R China Nanjing Agr Univ Coll Hort Nanjing Jiangsu Peoples R China

light is one of the most important environmental factors that affect plant growth and development. It also stimulates anthocyanin biosynthesis in plants. However, the precise molecular mechanisms through which light regulates anthocyanin biosynthesis, particularly in non-model plant species, remain poorly understood. In this study, we discovered a CmBBX28-CmMYB9a molecular module that is responsive to light and regulates anthocyanin biosynthesis in chrysanthemums. Specifically, CmBBX28 interacts with CmMYB9a, interfering with its binding to the promoters of target genes and reducing the protein abundance of CmMYB9a. This interaction downregulates the transcription of CmMYB9a's downstream anthocyanin-associated genes, CmCHS, CmDFR, and CmUFGT. The expression of CmBBX28 was induced in the dark, and the accumulated CmBBX28 proteins interfered with the activation of CmMYB9a during anthocyanin biosynthesis. Concurrently, darkness also inhibited the expression of CmMYB9a to some extent. In contrast, light significantly induced the expression of CmMYB9a and suppressed the expression of CmBBX28, resulting in increased anthocyanin accumulation in chrysanthemum petals. Our findings reveal the mechanism by which light regulates anthocyanin biosynthesis in chrysanthemum flower petals.

关键词： anthocyanin biosynthesis chrysanthemum CmBBX28 CmMYB9a light

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21世纪以来陈式太极拳技术体系演进特征

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武术研究 2019年第3期4卷 72-75页

作者：李楚文成都体育学院四川成都610041

文章采用文献资料法、逻辑分析法等,在大量搜集相关文献的基础上对其进行整理与分析,在此基础上对21世纪以前陈式太极拳的技术发展进行历史性回顾,分析了太极拳与武术的历史渊源及产生、形成与发展。探讨21世纪以来陈式太极拳技术体系... 详细信息

文章采用文献资料法、逻辑分析法等,在大量搜集相关文献的基础上对其进行整理与分析,在此基础上对21世纪以前陈式太极拳的技术发展进行历史性回顾,分析了太极拳与武术的历史渊源及产生、形成与发展。探讨21世纪以来陈式太极拳技术体系的演进历程与特征;分析这种发展趋势下陈式太极拳技术体系演变将面临的问题,提出有利于陈式太极拳发展的可行的技术体系的演变方向。

关键词： 21世纪陈式太极拳技术体系发展

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