Noninvasive genetic sampling greatly facilitates studies on the genetics, ecology, and conservation of threatened species. Species identification is often a prerequisite for noninvasive sampling-based biological studi...
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Noninvasive genetic sampling greatly facilitates studies on the genetics, ecology, and conservation of threatened species. Species identification is often a prerequisite for noninvasive sampling-based biological studies. Due to the low quantity and quality of genomic DNA from noninvasive samples, high-performance short-target PCR primers are necessary for DNA barcoding applications. The order Carnivora is characterized by an elusive habit and threatened status. In this study, we developed three pairs of short-target primers for identifying Carnivora species. The COI279 primer pair was suitable for samples with better DNA quality. The COI157a and COI157b primer pairs performed well for noninvasive samples and reduced the interference of nuclear mitochondrial pseudogenes (numts). COI157a could effectively identify samples from Felidae, Canidae, Viverridae, and Hyaenidae, while COI157b could be applied to samples from Ursidae, Ailuridae, Mustelidae, Procyonidae, and Herpestidae. These short-target primers will facilitate noninvasive biological studies and efforts to conserve Carnivora species.
We proposed a solution to improve the interface property and voltage instability of silicon carbide metal-oxide-semiconductor (SiC MOS) capacitors by eliminating near-interface defects with a single oxygen element. Re...
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We proposed a solution to improve the interface property and voltage instability of silicon carbide metal-oxide-semiconductor (SiC MOS) capacitors by eliminating near-interface defects with a single oxygen element. Results indicated that the quality of the silicon dioxide (SiO2) film and SiC/SiO2 interface and the voltage stability of SiC MOS capacitors were simultaneously improved by the proposed process. The mechanism underlying the improvement in the electrical performance of SiC MOS capacitors by oxygen plasma re-oxidation annealing (OP-ROA) could be elaborated by analysing the results of secondary ion mass spectroscopy and X-ray photoelectron spectroscopy. We believe that the OP-ROA process can eliminate C- and Si-related defects and silicon oxycarbide (SiOxCy) and reduce the thicknesses of the interface-transition region, thereby decreasing relative Si and C contents near the SiC/SiO2 interface. The evolution of C-related defect structures was simulated through density functional theory calculation, which was performed as a supplemental analysis.
Large language models (LLMs) have revolutionized the field of natural language processing, enabling remarkable progress in various tasks. Different from objective tasks such as commonsense reasoning and arithmetic que...
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Skeletonema marinoiis one of the most widespread marine planktonic diatoms in temperate coastal regions and sometimes can form massive blooms. Yet, the molecular mechanisms of triacylglycerol (TAG) synthesis in nutrie...
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Skeletonema marinoiis one of the most widespread marine planktonic diatoms in temperate coastal regions and sometimes can form massive blooms. Yet, the molecular mechanisms of triacylglycerol (TAG) synthesis in nutrient-deficient conditions for this species are still unknown. This study aimed to investigate how the TAG biosynthetic pathway ofS. marinoireacts to the culture age and nitrogen (N) or phosphorus (P) deficiency at molecular levels. Meanwhile, we also described the physiological and biochemical changes ofS. marinoiin response to N or P starvation over time. To obtain reliable qRT-PCR data, six putative reference genes were identified for assessing expression stability using geNorm and BestKeeper software, andActinexhibited the most stable expression across 45 ***. We found that the expression of TAG biosynthesis-related genes andACCaseenzyme activity varied in response to the different nutrient conditions and culture age. Taken together, we speculated that the capacity of TAG biosynthesis inS. marinoiis induced by N or P stress, and increases with culture age. Furthermore, TAG biosynthesis appears to respond more strongly to P deficiency than to N deficiency. Our study provides important insights into how diatoms regulate the TAG biosynthetic pathway when stressed by nutrient limitation. Besides, the data obtained from this study also provide useful clues for further exploring genes that can be used for metabolic engineering to enhance lipid production.
This paper is concerned with the resilient and secure remote monitoring of a cyber-physical system of a discrete time-varying state-space form against attacks. The specific statistical characteristic, magnitude, occur...
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This paper is concerned with the resilient and secure remote monitoring of a cyber-physical system of a discrete time-varying state-space form against attacks. The specific statistical characteristic, magnitude, occurring place and time of the attack signals are not required during the monitor design and attack detection procedures. First, an optimal ellipsoidal state prediction and estimation method is delicately developed in such a way that the recursively computed prediction ellipsoid and estimate ellipsoid can both guarantee the containment of the true system state at each time step regardless of the unknown but bounded input signal. It is expected that the two ellipsoids can resist certain attacks as the calculated state prediction and state estimate are sets in state-space rather than single pointwise vectors, thus potentially enhancing the resilience of the remote monitoring system. Second, a set-based evaluation mechanism in combination with a remedy measure are proposed to provide timely detection of certain attacks. Furthermore, a numerically efficient algorithm is established to achieve resilience and attack detection of the remote monitoring system. Finally, it is shown through several case studies on a water supply distribution system that the proposed methods can provide quantitative analysis and evaluation of the potential consequences of various attacks on the remote monitoring system. (C) 2019 Elsevier Inc. All rights reserved.
Propane dehydrogenation (PDH) provides a promising way to meet the requirement of propylene supply, but the commonly-used Pt-based catalysts still suffer from unsatisfactory stability and deactivation. Herein, an inte...
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Propane dehydrogenation (PDH) provides a promising way to meet the requirement of propylene supply, but the commonly-used Pt-based catalysts still suffer from unsatisfactory stability and deactivation. Herein, an interfacial regulation strategy is developed to construct the Cu, Pt, and Zn species modified hollow porous silicalite-1 (CuPt/ Zn-HPS-1). Owing to the regulation role of Pt, the electron-deficient [ZnOH]+ Lewis acid sites accelerated the CH activation, which was beneficial to achieve the excellent PDH performance. More importantly, the additionally introduced Cu2+ further modulated the geometric structure and electronic density of Pt, thus effectively improving the anti-sintering ability and alleviating the coke deposition. Benefiting from the interfacial regulation and Lewis active [ZnOH]+ sites, the optimal CuPt/Zn-HPS-1 catalyst exhibited an excellent catalytic PDH performance with the initial propane conversion of 30.4 % and propylene selectivity of 99.0 %. The CuPt/Zn-HPS-1 also demonstrated robust recycling stability in reaction-regeneration cycling test with the smaller deactivation rate constant. This work affords a strategy for improving PDH performance in practical industrial application.
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