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Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022年第41期70卷 13313-13326页

作者： Shi, Xiaolei Zhao, lina Niu, liyan Yan, Yixuan Chen, Qiushi Jin, Yongri li, xuwen Jilin Univ Coll Food Sci & Engn Changchun 130012 Peoples R China Jilin Univ Coll Chem Changchun 130012 Peoples R China

A new intervention was investigated for the induction of oral tolerance (OT) of OVA using narirutin by in vivo and in vitro experiments combined with network pharmacology and structural analysis of molecular docking. Narirutin (and its metabolism naringenin) has effects on OT by affecting B cell function, DCs, and T cell response by prediction. It was verified that narirutin could affect B cell function of secreting antibodies, thereby reducing the ability of DCs to absorb antigens by affecting GATA3, CCR7, STAT5, and MHCII expression and regulating T cell response by suppressing Th2 and improving Treg cells in vivo. Molecular docking showed that steric hindrance effects may be the reason for weaker binding energy with targets of narirutin. However, this does not mean that it has no bioactivity, for it can inhibit mast cell degranulation. This finding is interesting because it offers the possibility of using natural compounds to promote oral tolerance.

关键词： oral tolerance narirutin food allergy OVA intervention network pharmacology

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Magnetic dispersive solid-phase extraction for the determination of three different glycosides in the Kang'ai injection

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MICROCHEMICAL JOURNAL 2019年 146卷 575-581页

作者： li, Peng Ye, Junfeng Zhang, Yue Wang, Ziqi Ren, Sujuan Jin, Yongri li, xuwen Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China Jilin Univ Hosp 1 Changchun 130012 Jilin Peoples R China

In this work, the content of flavonoid glycosides and astragalosides in a Chinese medicine injection was determined by using the magnetic dispersive solid phase extraction method coupled with high performance liquid chromatography with an evaporative light-scattering detector (HPLC/ELSD). Multi-walled carbon nanotubes (MWCNTs) modified by Fe3O4 nanoparticles obtained in a one-step synthetic procedure, were used as a sorbent to conveniently remove the analytes from water. Single factor experiments were performed to investigate the factors that affected the extraction efficiency, such as the hydroxyl content of the modified MWCNTs, the amount of sorbent, the type of elution solvent, the volume of elution solvent, sample pH and salting-out effect. Under the appropriate optimal conditions, the limits of detection (LODs) and limits of quantification (LOQs) for the three glycosides ranged from 0.16 mu g/mL to 0.60 mu g/mL and 0.58 mu g/mL to 1.24 mu g/mL, respectively. Moreover, the intra- and interday precision ranged from 1.9% to 2.9% and 2.4% to 4.5%, respectively. Meanwhile, the recoveries of spiked samples were measured, which ranged from 91.1% to 96.2% with relative standard deviations (RSDs) ranging from 2.8% to 3.8%. Accordingly, the application of the proposed method for the analysis of Chinese medicine injection samples offered several advantages: an acceptable precision, low LODs and good recoveries. This work might provide a research basis for the quality control of Chinese medicine injections and the further study of the Kang'ai injection.

关键词： Dispersive solid phase extraction Evaporative light-scattering detector Kang'ai injection

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Semisynthesis and Cytotoxicity Evaluation of a Series of Ocotillol Type Saponins and Aglycones from 20（S）-Ginsenoside Rg2, Rhl, Protopanaxatriol and Their 20（R）-Epimers

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Chemical Research in Chinese Universities 2016年第1期32卷 35-40页

作者： YANG Jie YU Xue CAI Xiaoxiang CHEN Yanxin ZANG Huiming li xuwen JIN Yongri College of Chemistry Jilin University Changchun 130021 P. R. China College of Life Sciences Jilin University Changchun 130012 P. R. China Hangzhou Innovative Chinese Medicine Standardization Research Institute Co. Ltd. Hangzhou 310053 P. R. China

Abstract With the oxidation treatment, eighteen compounds were separated from 20（S）-ginsenoside Rg2, Rhl, pro- topanaxatriol（PPT） and their 20（R）-epimers in total and cytotoxicity of most of them was evaluated against three hu- man cancer cell lines HeLa, A549 and MCF-7 by 3-（4,5-dimetylthiazol-z-yl）-2,5-diphenyltetrazolium bromide（MTT） assay. Their structures were confirmed by means of nuclear magnetic resonance（NMR） and mass spectrometry and the results were compared with those of previous literature. In this study, we systematically semisynthesized all four ocotillol type saponins, i.e., （20S, 24S＇）, （20S, 24R）, （20R, 24S） and （20R, 24R）. All the configurations at C20 kept the same with their starting materials. Meanwhile a pair of C24 epimers was generated in terms of ocotillol type saponins In addition, seven compounds（4--8, 13 and 14） were reported firstly. The cytotoxic results distinguished the ocotillol type products（6, 7, 13 and 14） from 20（R）-PPT and 20（R）-ginsenoside Rhl, which possessed better cytotoxicities than their correspondents from 20（S）-epimers against HeLa cells, and the carbonyl group at C3 can improve the cytotoxi- city, which helped us to gain deeper insight into Ocotillol type saponins.

关键词： Semisynthesis Ocotillol Saponin Cytotoxicity

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A FRET-based fluorescent probe for mercury ions in water and living cells

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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2016年 165卷 99-105页

作者： Zhang, Bo Ma, Pinyi Gao, Dejiang Wang, Xinghua Sun, Ying Song, Daqian li, xuwen Jilin Univ Coll Chem Qianjin St 2699 Changchun 130012 Peoples R China Changchun Jilin Univ Little Swan Instruments Co Ltd Chuangxin Rd 1203 Changchun 130012 Peoples R China

On the basis of fluorescence resonance energy transfer (FRET), a new rhodamine derivative (DRh) was synthesized as a ratiometric fluorescent probe for detecting Hg2+ in water and living cells samples. The recognition properties of the probe DRh with metal ions had been investigated in H2O/CH3CN (9:1, v/v;Tris-HCl 50 mmol L-1;pH = 7.0) solution by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The results showed that the probe DRh exhibited the selective recognition of Hg2+. Upon the addition of Hg2+, the spirolactam ring of probe DRh was opened. The 1:1 stoichiometric structure between DRh and Hg2+ were supported by Job's plot, MS and OFT theoretical calculations. The linearly fluorescence intensity ratio (I-582/I-538) is proportional to the concentration of Hg2+ in the range 0-30 mu mol L-1. The limit of detection (LOD) of Hg2+ is 0.008 mu mol L-1 (base on S/N = 3). The present probe was applied to the determination of Hg2+ in neutral water samples and gave recoveries ranging from 104.5 to 107.9%. Furthermore, the fluorescent probe also can be applied as a bioimaging reagent for Hg2+ detection in HeLa cells. (C) 2016 Elsevier B.V. All rights reserved.

关键词： Mercury ions Fluorescence probe Rhodamine FRET Water living cells DFT

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Polyoxygenated anti-inflammatory biscembranoids from the soft coral Sarcophyton tortuosum and their stereochemistry

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Chinese Chemical Letters 2021年第1期32卷 271-276页

作者： Yufen li Songwei li Cristina Cuadrado Chenglong Gao Qihao Wu Xiaolu li Tao Pang Antonio Hernandez Daranas Yuewei Guo xuwen li State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China School of Chinese Materia Medica Nanjing University of Chinese MedicineNanjing 210023China Instituto de Productos Naturalesy Agrobiologi’a Consejo Superior de Investigaciones Científicas(IPNA-CSIC)La Laguna 38206Spain Jiangsu Key Laboratory of Drug Screening State Key Laboratory of Natural MedicinesJiangsu Key Laboratory of Drug Discovery for Metabolic DiseasesChina Pharmaceutical UniversityNanjing210009 China

Five novel biscembranoids,ximaolides H-L(1-5),along with four known related compounds(6-9) were isolated from the Hainan soft coral Sarcophyton *** structures of the new compounds were determined by extensive spectroscopic analysis,quantum chemical calculations,and/or by comparing their CD spectra with those of the known *** 1 and 2 are the first examples of biscembranoids bearing a 1,35-bridged lactone moiety,4 is the first biscembranoid comprising an uncommon oxetane ring,and 5 represents the first 36-peroxyl *** Ⅰ(2),K(4) and F(9) exhibited interesting anti-inflammatory activity by the inhibition of LPS-induced TNF-α protein release in RAW264.7 macrophages.

关键词： Soft coral Sarcophyton tortuosum Biscembranoid Stereochemistry Anti-inflammatory

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In Vitro Study of the Binding of Taxifolin to Bovine Serum Albumin and the Influence of Common Ions on the Binding

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JOURNAL OF SOLUTION CHEMISTRY 2010年第4期39卷 482-494页

作者： Shi, Xiaolei li, xuwen Sun, Yantao Wei, Wei Yang, Ruijie Zhang, Hanqi Jin, Yongri Jilin Univ Coll Chem Changchun 130012 Peoples R China

The interaction between taxifolin and bovine serum albumin (BSA), and the effects of some common ions on their interaction, were investigated by fluorescence and UV-visible absorption spectroscopy. The results indicate that taxifolin has a strong ability to quench the intrinsic fluorescence of BSA through a static quenching process. According to values of the thermodynamic parameters, the hydrophobic force plays a major role in the interaction. Based on FAster's non-radiation theory, the energy transfer distances between BSA and taxifolin in the absence and presence of some common ions were obtained. The experimental results indicate that the transfer distances are almost unaffected by these ions. The conformation of BSA undergoes significant change from the formation of a taxifolin-BSA complex, which was studied by synchronous and three-dimensional fluorescence spectroscopy.

关键词： Interaction Taxifolin Bovine serum albumin Quenching of fluorescence Common ions

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A multi-model object tracking algorithm based on texture and color features 8

A multi-model object tracking algorithm based on texture and...

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8th International Conference on Computing and Pattern Recognition, ICCPR 2019

作者： li, xuwen Xue, Wendong Shi, lijuan Wu, Qiang Beijing University of Technology College of Life Science and Bioengineering Beijing China

ISBN: (纸本)9781450376570

Mean Shift is widely concerned because of its advantages like fast convergence, real-time performance and simple procedure. However, the tracking performance of the traditional Mean Shift algorithm is obviously interfered when the background has the similar color as the target or the illumination changes. Besides, the tracking is easy to defeat in the case of target occlusion and loss of important frames. Therefore, this paper proposes two improvements based on the traditional Mean Shift tracking algorithm. First, the HLBP texture feature and color feature are employed to describe the target feature in order to improve the robustness of the tracking algorithm. Second, multiple models are taken into account to provide more abundant choices for the tracking process which can improve the tacking performance. The result of experiments show that our algorithm is more robust under the case of object occlusion and posture change, and gets better performance in accuracy when the background color and target color are similar or the illumination changes. © 2019 Association for Computing Machinery.

关键词： Color

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A review: The structures and bioactivities of steroidal saponins from Allium macrostemon Bulbus

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PHYTOCHEMISTRY LETTERS 2023年第1期57卷 210-226页

作者： Xie, Huili Shi, Xiaolei Zhao, Dexin Wang, Bingzi Jin, Yongri li, xuwen Jilin Univ Coll Chem 2519 Jiefang Rd Changchun 130021 Peoples R China Jilin Univ Coll Food Sci & Engn Changchun 130062 Peoples R China

Allium macrostemon Bulbus (Xiebai in Chinese), as an important medicinal and edible homologous plant, has two kinds of original plants (the dry bulbs of Allium macrostemon and Allium chinense). In China, the dry bulbs of Allium macrostemon and Allium chinense have been used to treat some diseases for thousands of years. Steroidal saponins are considered to be one type of their most important active ingredients and research into the phar-macological activities of these compounds are currently undertaken. Some steroidal saponins may be promising therapeutic tools, which has caught the attention of researchers. This article provides a comprehensive overview of 102 steroidal saponins and sapogenins found in Allium macrostemon Bulbus, along with their reported phar-macological activities, including anti-inflammatory, antioxidant, antiplatelet aggregation, blood lipid-lowering, antitumor, and other beneficial effects. In this review, the extraction and isolation, spectral characteristics and pharmacological activities of steroidal saponins have been summarized based on literature from 1989 to 2022.

关键词： Allium macrostemon Bulbus Allium macrostemon Allium chinense Steroidal Saponins Chemical structure Bioactivity

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Centrifugation free and air-assisted liquid-liquid microextraction based on deep eutectic solvent for determination of rare ginsenosides in Kang'ai injection

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MICROCHEMICAL JOURNAL 2018年 142卷 313-320页

作者： li, Peng Ye, Junfeng Zhang, Yue Wang, Ziqi Ren, Sujuan li, xuwen Jin, Yongri Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China Jilin Univ Hosp 1 Changchun 130012 Jilin Peoples R China

A simple, effective and convenient enrichment method coupled with high performance liquid chromatography (HPLC) was established for the analysis of rare ginsenosides in Kang'ai (KA) injection. Air assisted liquid-liquid microextraction was used in this work for the first time to determine the rare ginsenosides in KA injection. Deep eutectic solvent (DES) and tetrahydrofuran (THF) were specially selected as a novel green extraction solvent and an emulsified solvent, respectively. To remove the DES droplets, Fe3O4 nanoparticles were used. Therefore, centrifugation was free in this work, which can increase the efficiency of analysis procedure. Moreover, Fe3O4 nanoparticles were reusable. To achieve better extraction efficiency and enrichment factor, the experimental conditions have been optimized, including type and volume of DES and emulsified solvent, amount of Fe3O4 nanoparticles and inorganic salt, and pH value. Under the optimum extraction condition, the validation parameters show the limits of detection (LODs) are in the range from 10.2 ng/mL to 137.8 ng/mL and the limits of quantity (LOQs) are in the range from 55.5 ng/mL to 428.5 ng/mL. The recoveries of analytes range from 91.3% to 106.7% with the relative standard deviations (RSDs) ranging from 1.2% to 4.5%. The recommended approach was utilized to acquire the information of constituents in KA injection. Moreover, the results in this research could provide a study basis for further study of KA injection.

关键词： Deep eutectic solvent liquid-liquid microextraction Rare ginsenosides Kang'ai injection HPLC

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An efficient strategy based on two-stage chromatography and in vitro evaluation for rapid screening and isolation of acetylcholinesterase inhibitors from Scutellaria baicalensis Georgi

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JOURNAL OF SEPARATION SCIENCE 2022年第6期45卷 1170-1184页

作者： Hou, Wanchao liu, Chunming li, Sainan Zhang, Yuchi Jin, Yongri li, xuwen liu, Zhen Niu, Huazhou Xia, Jianli Jilin Univ Coll Chem Changchun Jilin Peoples R China Changchun Normal Univ Cent Lab Changchun Jilin Peoples R China

The extraction of Scutellaria baicalensis Georgi was investigated using the response surface methodology-genetic algorithm mathematical regression model, and the extraction variables were optimized to maximize the flavonoid yield. Furthermore, a simple and efficient ultrafiltration-liquid chromatography-mass spectrometry and molecular docking methods were developed for the rapid screening and identification of acetylcholinesterase inhibitors present in Scutellaria baicalensis Georgi. Subsequently, four major chemical constituents, namely baicalein, norwogonin, wogonin, and oroxylin A, were identified as potent acetylcholinesterase inhibitors. This novel approach, involving the use of ultrafiltration-liquid chromatography-mass spectrometry and molecular docking methods combined with stepwise flow rate counter-current chromatography and semi-preparative high-performance liquid chromatography, could potentially provide a powerful tool for the screening and extraction of acetylcholinesterase inhibitors from complex matrices and be a useful platform for the production of bioactive and nutraceutical ingredients.

关键词： acetylcholinesterase inhibitors countercurrent chromatography mathematical model purification Scutellaria baicalensis Georgi

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