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MALDI-TOF mass spectrometry-based serotyping of V-parahaemolyticus isolated from the Zhejiang province of china

BMC MICROBIOLOGY 2018年第1期18卷 1-10页

作者： Li, Ping Xin, Wenwen Xia, Susu Luo, Yun Chen, Zhongwen Jin, Dazhi Gao, Shan Yang, Hao Ji, Bin Wang, Henghui Yan, Yong Kang, Lin Wang, Jinglin Anhui Med Univ Grad Coll 81 Meishan Rd Hefei 230032 Anhui Peoples R China Jiaxing Ctr Dis Control & Prevent Jiaxing Key Lab Pathogen Microbiol 486 Wenqiao Rd Nanhu Dist 314050 Jiaxing Peoples R China Beijing Inst Microbiol & Epidemiol State Key Lab Pathogen & Biosecur 20 Dongdajie Beijing 100071 Peoples R China Zhejiang Prov Ctr Dis Control & Prevent Dept Microbiol 3399 Binsheng Rd Hangzhou 310000 Zhejiang Peoples R China

BackgroundVibrio parahaemolyticus is as an important food-borne pathogen circulating in china. Since 1996, the core serotype has become O3:K6, which has specific genetic markers. This serotype causes the majority of outbreaks worldwide. Until now, nearly 21 serotypes were considered as serovariants of O3:K6. Among these, O4:K68, O1:K25 and O1:KUT have caused pandemic outbreaks. O4:K8, a serovariant of O3:K6, has become the second most dominant serotype circulating in china after O3:K6. In this study, we report the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze and characterize 146V. parahaemolyticus isolates belonging to 23 *** mass spectral analysis, isolates belonging to O4:K8 formed a distinct group among the five main pandemic groups (O3:K6, O4:K8, O4:K68, O1:K25 and O1:KUT). Two major protein peaks (m/z 4383 and 4397) were significantly different between serotype O4:K8 and the four other pandemic strains. Both of these peaks were present in 32 out of 36 O4:K8 isolates, but were absent in 105 out of 110 non-O4:K8 isolates. These peaks were also absent in all 74 pandemic serotypes (O3:K6, O4:K68, O1:K25 and O1:KUT).ConclusionOur results highlight the threat of O4:K8 forming a distinct group, which differs significantly from pandemic serotypes on the proteomic level. The use of MALDI-TOF MS has not been reported before in a study of this nature. Mass spectrum peaks at m/z 4383 and 4397 may be specific for O4:K8. However, we cannot conclude that MALDI-TOF MS can be used to serotype V. parahaemolyticus.

关键词： Vibrio parahaemolyticus MALDI-TOF MS O4:K8 Biomarkers

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CALCINATION OF ALUMINUM CHLORIDE HEXAHYDRATE (ACH) FOR ALUMINA PRODUCTION: IMPLICATIONS FOR ALUMINA EXTRACTION FROM ALUMINUM RICH FLY ASH (ARFA)

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ARChiVES OF METALLURGY AND MATERIALS 2018年第1期63卷 235-240页

作者： Zhao, L. Natl Inst Clean & LOW Carbon Energy Xiaotangshan Future Sci & Technol City POB 001 Shenhua Nice Beijing 102211 Peoples R China

Alumina rich fly ash (ARFA) has been regarded as the alternative to bauxite in china. Hydrochloric acid process could be favored for alumina extraction, necessitating calcination of aluminum chloride hexahydate (ACH). In this work, the TGA/DSC results of ACH were used to suggest calcination procedures. Two-step calcinations of 200-1000 degrees C and 350-1000 degrees C did not increase the surface area of alumina, by comparison with one step 1000 degrees C calcination, and a slow heating rate could improve the surface area. Calcination temperature was increased from 950 to 1250 degrees C in a step of 50 degrees C, and XRD, XRF, BET and gas pycnometer were used to characterize the alumina from calcinated ACH. Consistent results were obtained by these different techniques, and two groups of impurities were identified and related to alumina purity and surface area. By comparison with clays, it was suggested to remove impurities such as MgO, Na2O, K2O, P2O5 and SO3 in hydrochloric acid leaching of ARFA.

关键词： calcination aluminum chloride hexahydrate alumina rich fly ash acid leaching

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Extended Application Of Time-Varying Observer For Rigid Rotors Unbalance Estimation During Acceleration 3

Extended Application Of Time-Varying Observer For Rigid Roto...

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3rd International Conference on Mechanical, Manufacturing, Modeling and Mechatronics (IC4M) / 3rd International Conference on Design, Engineering and Science (ICDES)

作者： Yu, Xunxing Mao, Kuanmin Zhu, Yaming Huazhong Univ Sci & Technol Sch Mech Sci & Engn Wuhan 430074 Hubei Peoples R China

Unbalance is one of essential problems for modern rotating machines. In this work, an improved time-varying observer is proposed to estimate the unbalance of rigid rotor during acceleration. In order to fitting different speed acceleration laws, the unbalance forces have been included in an new designed augmented states, meanwhile the state space model of rigid rotor has been also developed. The developed state space model is transformed to a canonical transformation and a new designed time-varying observer can be obtained. The estimated unbalances can be directly obtained by using this time-varying observer. This method would be very helpful for active balancing control strategy during acceleration.

关键词： State space methods

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Automatic 2-D/3-D Vessel Enhancement in Multiple Modality Images Using a Weighted Symmetry Filter

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IEEE TRANSACTIONS ON MEDICAL IMAGING 2018年第2期37卷 438-450页

作者： Zhao, Yitian Zheng, Yalin Liu, Yonghuai Zhao, Yifan Luo, Lingling Yang, Siyuan Na, Tong Wang, Yongtian Liu, Jiang Chinese Acad Sci Ningbo Inst Ind Technol Cixi Inst Biomed Engn Ningbo 315201 Zhejiang Peoples R China Beijing Inst Technol Sch Opt & Elect Beijing Engn Res Ctr Mixed Real & Adv Display Beijing 100081 Peoples R China Univ Liverpool Dept Eye & Vis Sci Liverpool L69 3BX Merseyside England Aberystwyth Univ Dept Comp Sci Aberystwyth SY23 3FL Dyfed Wales Cranfield Univ EPSRC Ctr Innovat Mfg Through Life Engn Serv Cranfield MK43 0AL Beds England Georgetown Preparatory Sch North Bethesda MD 20852 USA

Automated detection of vascular structures is of great importance in understanding the mechanism, diagnosis, and treatment of many vascular pathologies. However, automatic vascular detection continues to be an open issue because of difficulties posed by multiple factors, such as poor contrast, in homogeneous backgrounds, anatomical variations, and the presence of noise during image acquisition. In this paper, we propose a novel 2-D/3-D symmetry filter to tackle these challenging issues for enhancing vessels from different imaging modalities. The proposed filter not only considers local phase features by using a quadrature filter to distinguish between lines and edges, but also uses the weighted geometric mean of the blurred and shifted responses of the quadrature filter, which allows more tolerance of vessels with irregular appearance. As a result, this filter shows a strong response to the vascular features under typical imaging conditions. Results based on eight publicly available datasets (six 2-D data sets, one 3-D data set, and one 3-D synthetic data set) demonstrate its superior performance to other state-of-the-art methods.

关键词： Symmetry filter local phase vascular enhancement angiography

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Learning an identity distinguishable space for large scale face recognition

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The Journal of china Universities of Posts and Telecommunications 2018年第1期25卷 54-61页

作者： Yue Ting Wang Hongbo Cheng Shiduan State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications

Implementing face recognition efficiently to real world large scale dataset presents great challenges to existing approaches. The method in this paper was proposed to learn an identity distinguishable space for large scale face recognition in MSR-Bing image recognition challenge （IRC）. Firstly, a deep convolutional neural network （CNN） was used to optimize a 128 B embedding for large scale face retrieval. The embedding was trained via using triplets of aligned face patches from FaceScrub and CASIA-WebFace datasets. Secondly, the evaluation of MSR-Bing IRC was conducted according to a cross-domain retrieval scheme. The real-time retrieval in this paper was benefited from the K-means clustering performed on the feature space of training data. Furthermore, a large scale similarity learning （LSSL） was applied on the relevant face images for learning a better identity space. A novel method for selecting similar pairs was proposed for LSSL. Compared with many existing networks of face recognition, the proposed model was lightweight and the retrieval method was promising as well.

关键词： face recognition convolutional neural network （CNN） triplet loss inception similarity learning

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The effect of phosphate buffer on improving the performance of autothermal thermophilic aerobic digestion for sewage sludge

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RSC ADVANCES 2018年第17期8卷 9175-9180页

作者： Jin, Ningben Shanghai Environm Sanitat Engn Design Inst Co Ltd Shanghai 200232 Peoples R China

The influence of phosphate buffer on the stabilization of sewage sludge was investigated in autothermal thermophilic aerobic digestion (ATAD). A concentration series of 0.005, 0.01, 0.02 and 0.03 mol phosphate buffer for each liter of sludge was adopted. The phosphate buffer significantly enhanced the performance of the ATAD for sewage sludge. The highest VS removal was achieved by the group with 0.01 mol L-1 phosphate buffer, and the stabilization time of the sludge was shortened by 9 days compared with that of the control. The group with the optimal dosage obtained the deepest stabilization level of sludge, which was reflected by the distribution of the particle size, and achieved 6.08% VS removal higher than that of the control in the end. Lower concentrations of carbon, nitrogen and phosphate in the supernatant were also achieved by proper dosing compared with those of the control.

关键词： Sewage sludge

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New Calibrator with Points Distributed Conical Helically for Online Calibration of C-Arm

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SENSORS 2019年第9期19卷 1989.-1989.页

作者： Guo, Na Yang, Biao Wang, Yuhan Liu, Hongsheng Hu, Lei Wang, Tianmiao Beihang Univ Sch Mech Engn & Automat Beijing 100083 Peoples R China

To improve the accuracy of calibration of C-arm, and overcome the space limitation in surgery, we proposed a new calibrator for online calibration of C-arm. After the image rectification by a polynomial fitting-based global correction method, the C-arm was assumed as an ideal pinhole model. The relationships between two kinds of spatial calibration errors and the distribution of fiducial points were studied: the performance of FRE (Fiducial Registration Error) and TRE (Target Registration Error) were not consistent, but both were best at the 12 marked points;the TRE decreased with the increase of the uniformity of calibration points distribution, and with the decrease of the distance between the target point and the center of calibration points. A calibrator with 12 fiducial points conical helically distributed, which could be placed on the knee, was an attractive option. A total of 10 experiments on C-arm calibration accuracy were conducted and the mean value of mapping error was 0.41 mm. We designed an ACL reconstruction navigation system and carried out specimen experiments on 4 pairs of dry femur and tibia. The mean accuracy of navigation system was 0.85 mm, which is important to the tunnel positioning for ACL reconstruction.

关键词： surgical navigation system online C-arm calibration method calibration error ACL reconstruction

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Start up of anammox process with activated sludge treating high ammonium industrial wastewaters as a favorable seeding sludge source

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INTERNATIONAL BIODETERIORATION & BIODEGRADATION 2018年 127卷 17-25页

作者： Chi, Yong-Zhi Zhang, Yu Yang, Min Tian, Zhe Liu, Ru-Yin Yan, Feng-Ying Zang, Ya-Nan Chinese Acad Sci Res Ctr Ecoenvironm Sci State Key Lab Environm Aquat Chem Beijing 100085 Peoples R China Tianjin Chengjian Univ Sch Environm & Municipal Engn Tianjin 300384 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China

It is a big challenge to select suitable seeding sludge for the quick startup of anaerobic ammonium oxidation (anammox) systems. Six seeding sludge originating from three municipal wastewater treatment plants (WWTPs) and three industrial WWTPs with high influent ammonium concentrations (landfill, coking and antibiotic production wastewater) were selected to explore suitable seeding sludge for the startup of anammox systems. The abundances of anammox bacteria in the six seeding sludge were characterized by quantitative PCR targeting the hydrazine synthase beta subunit (hzsB) genes, and industrial sludge samples exhibited relatively higher abundances of anammox bacteria than the municipal ones. The logarithm of anammox bacterial abundance in seeding sludge was significantly correlated with the influent ammonium concentration (p < 0.05). Start up performance was evaluated for five (three municipal and two industrial sources) of the six sludge samples in semi-batch mode for 175 d. It took 18-86 d for the five sludge to exhibit detectable anammox activity, with two industrial source sludge (coking wastewater and landfill leachate) exhibiting the shortest start up times (18-33 d). Cloning analysis showed that all of the five final anammox sludge were dominated by Brocadia or Brocadia-like species. This study showed that industrial WWTP sludge treating ammonium rich wastewater might be a good candidate seeding source for the quick startup of anammox process.

关键词： Anammox Start up Industrial wastewater Seeding sludge Anaerobic ammonium-oxidizing bacteria

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A Laterally Driven MEMS Inertial Switch With Double-Layer Suspended Springs for Improving Single-Axis Sensitivity

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IEEE TRANSACTIONS ON COMPONENTS PACKAGING AND MANUFACTURING techNOLOGY 2018年第10期8卷 1845-1854页

作者： Yang, Zhuoqing Shi, Jianhao Yao, Jinyuan Zhang, Xiaojing Ding, Guifu Zhao, Xiaolin Shanghai Jiao Tong Univ Natl Key Lab Sci & Technol Micro Nano Fabricat Sch Elect Informat & Elect Engn Shanghai 200240 Peoples R China

A novel laterally driven inertial switch with double-layer springs has been proposed and fabricated by surface micromachining technology for improving single-axial sensitivity. The symmetrical distribution of double-layer suspended springs and the constraint structures limits the displacement of the proof mass in the off-axis sensitive direction under an acceleration disturbance. The ANSYS simulation results reveal that compared with the inertial switch with one layer springs, the symmetrical distribution of double-layer serpentine springs effectively reduces the displacement of the proof mass in the off-axis direction. The design of symmetrical distribution of double-layer serpentine springs plays an important role in resisting small acceleration disturbance from the off-axis sensitive z-direction, and the constraint structures can resist the large acceleration disturbance. The modal analysis, contact time, and the collision response in the inertial switch have also been simulated and discussed. Finally, the proposed inertial switch has been fabricated successfully, and the prototype is tested by a dropping hammer system. The test results show that the threshold level of the fabricated inertial switch is 272 g with 20-mu s contact time. The combined efforts of double-layer suspended springs and constraint structures effectively lower off-axis sensitivity and improve the single-axis sensitivity of the microelectromechanical system inertial switch.

关键词： Axial sensitivity double-layer springs inertial switch microelectromechanical system (MEMS)

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Design and characterization of an inertial microswitch with synchronous follow-up flexible compliant electrodes capable of extending contact duration

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SENSORS AND ACTUATORS A-PHYSICAL 2018年 270卷 34-45页

作者： Xu, Qiu Sun, Bin Li, Yigui Xiang, Xiaojian Lai, Liyan Li, Jian Ding, Guifu Zhao, Xiaolin Yang, Zhuoqing Shanghai Jiao Tong Univ Sch Elect Informat & Elect Engn Natl Key Lab Sci & Technol Micro Nano Fabricat Shanghai 200240 Peoples R China Chinese Acad Sci Shenzhen Inst Adv Technol Inst Biomed & Hlth Engn Shenzhen 518055 Peoples R China Shanghai Inst Technol Sch Sci Shanghai 201418 Peoples R China

An inertial microswitch with synchronous follow-up compliant electrodes for extending the contact duration is proposed in this paper. A flexible movable electrode and a stationary electrode in the double-stair and spring-shape structures are used for not only extending contact duration but also reducing the impact bounces. The simulated contact time can reach 350 mu s under 500 g applied acceleration, which indicates there is a better contact effect than that reported elsewhere. The fabricated inertial microswitch based on surface micromachining technology was characterized by the dropping hammer system. No contact bouncing behavior can be observed under the acceleration amplitude 466 g and the test contact duration can reach 390 mu s, longer than the microwitch with only one flexible electrode. The testing comparison of the microswitches with and without constraint structure indicates that the contact-bouncing behavior is easier to happen if without constraint structure, which indicates that the designed constraint structure can restrain the vibration of the proof mass in z-off-axis sensitive direction and effectively improve the stability of the device. The test results have an agreement with the simulated ones. (C) 2017 Elsevier B.V. All rights reserved.

关键词： Inertial microswitch Surface micromachining Bounce behavior Contact duration

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