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p27 inhibits CDK6/CCND1 complex formation resulting in cell cycle arrest and inhibition of cell proliferation

CELL CYCLE 2018年第19-20期17卷 2335-2348页

作者： Li, Niannian Zeng, Jie Sun, Fuze Tong, Xiaoling Meng, Gang Wu, Chunman Ding, Xin Liu, Lanlan Han, Minjin Lu, Cheng Dai, Fangyin Southwest Univ Key Lab Sericultural Biol & Genet Breeding State Key Lab Silkworm Genome Biol Minist AgrColl Biotechnol Chongqing Peoples R China

p27 plays critical roles in cell proliferation, differentiation, and apoptosis, which have been well studied in mammals and Drosophila. However, the mechanisms underlying p27 regulation of the cell cycle have not been thoroughly researched. In this study, Genevestigator, Kaplan-Meier Plotter, and the Human Protein Atlas databases were used to analyze the expression of p27, cell division protein kinase 6 (CDK6), and cyclin D1 (CCND1), as well as its prognostic value in different tumor tissues and corresponding normal tissues. Quantitative PCR and immunohistochemistry were used to detect the expression of p27, CDK6, and CCND1 in the tissues of cancer patients. The effects of p27, CDK6, and CCND1 on the proliferation of lung cancer cells were examined by the MTT assay, and flow cytometry was used to investigate the mechanism by which p27 affected cell proliferation. Immunofluorescence, co-immunoprecipitation, and Western blotting were used to determine if p27 interacted with CDK and CCND1 to regulate the cell cycle. The results showed that p27, CDK6, and CCND1 played different roles in tumorigenesis and development, which are in accordance with CDK6 and CCND1 in affecting the cell cycle and cell proliferation. p27 regulated the cell cycle and inhibited cell proliferation by affecting formation of the cell cycle-dependent complex CDK6/CCND1, but did not directly affect the expression of CDK6 and CCND1. Moreover, CCND1 did not regulate the cell cycle alone, but rather, functioned together with CDK6. This study provides insights into the effects of p27 on tumor formation and development, and the underlying regulatory mechanisms.

关键词： Cancer cell proliferation cell cycle p27 CDK6 CCND1

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Bifunctional Super-resolution Imaging Probe with Acidity-Independent Lysosome-Retention Mechanism

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ANALYTICAL CHEMISTRY 2018年第19期90卷 11393-11400页

作者： Xue, Zhongwei Wang, Siyu Li, Jian Chen, Xin Han, Jiahuai Han, Shoufa Xiamen Univ MOEDept Chem Biol Key Lab Spectrochem Anal & InstrumentatColl Chem Key Lab Chem Biol Fujian ProvState Key Lab Phys Xiamen 361005 Peoples R China Xiamen Univ Innovat Ctr Cell Signaling Network Xiamen 361005 Peoples R China Xiamen Univ Innovat Ctr Cell Signaling Network Sch Life Sci State Key Lab Cellular Stress Biol Xiamen 361005 Peoples R China

Spatiotemporal imaging is of enormous use to explore organelle biology, necessitating organelle-tracing techniques reliable in varied cell stress. We herein reported lysosomal imaging using rhodamine-X-integrated sialic acid ((ROX)SA), which is stably maintained in lysosomes irrespective of lysosomal pH changes. Exhibiting bright fluorescence and superior photostability, (ROX)SA enables 120 h continual tracking of fusion/ fission of lysosomes and mitochondrion-lysosome interaction in mitophagy. Relative to conventional acidotropic probes prone to dissipation from stressed lysosomes, (ROX)SA offers a new route for long-term tracking of stressed lysosomes relevant to diverse pathological conditions.

关键词： Fluorescence microscopy Super-resolution imaging Fluorescence Fluorescence imaging Probes

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Organelle-Directed Staudinger Reaction Enabling Fluorescence-on Resolution of Mitochondrial Electropotentials via a Self-Immolative Charge Reversal Probe

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ANALYTICAL CHEMISTRY 2018年第4期90卷 2954-2962页

作者： Xue, Zhongwei Zhu, Rui Wang, Siyu Li, Jian Han, Jiahuai Liu, Jian Han, Shoufa Xiamen Univ State Key Lab Phys Chem Solid Surfaces MOE Key Lab Spectrochem Anal & Instrumentat Dept Chem BiolColl Chem & Chem EngnKey Lab Chem Xiamen 361005 Peoples R China Xiamen Univ Innovat Ctr Cell Signaling Network Xiamen 361005 Peoples R China Xiamen Univ Sch Life Sci Innovat Ctr Cell Signaling Network State Key Lab Cellular Stress Biol Xiamen 361005 Peoples R China Lanzhou Univ Sch Pharm Lanzhou 73000 Gansu Peoples R China

Organelles often feature parameters pertinent to functions and yet responsive to biochemical stress. The electro-potential across the mitochondrial membrane (Delta psi m) is a crucial mediator of cell fates. Herein we report a bioorthogonal reaction enabled fluorescence-on probing of Delta psi m alterations featuring anionic fluorescein-triphenylphosphonium diad (F-TPP), which is released via intramitochondria Staudinger reaction triggered self-immolation of o-azidomethylbenzoylated F-TPP. Compared to classical cationic mitochondria-specific dyes, F-TPP is hydrophilic and negatively charged. Effectively discerning Delta psi m changes upon diverse stress inducers, the organelle directed bioorthogonal imaging strategy offers unprecedented choices to probe mitochondrial biology with functional molecules that are otherwise inaccessible via physiological organelle probe affinity.

关键词： Dyes and pigments Fluorescence Fluorescence imaging Heterocyclic compounds Mitochondria

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Frequency Response of a Close-Loop Bell-Bloom Magnetometer 72

Frequency Response of a Close-Loop Bell-Bloom Magnetometer

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IEEE International Frequency Control Symposium (IFCS)

作者： Zhang, Rui Pang, Bo Li, Wenhao Yang, Yucheng Chen, Jingbiao Peng, Xiang Guo, Hong Peking Univ State Key Lab Adv Opt Commun Syst & Network Sch Elect Engn & Comp Sci Beijing 100871 Peoples R China Peking Univ Ctr Quantum Informat Technol Beijing 100871 Peoples R China Natl Univ Def Technol Coll Liberal Arts & Sci Changsha 410073 Hunan Peoples R China Natl Univ Def Technol Interdisciplinary Ctr Quantum Informat Changsha 410073 Hunan Peoples R China

ISBN: (纸本)9781538632147

In this article, the dependence of the frequency response of a close-loop Bell-Bloom magnetometer on its various experimental parameters is studied. With a proper setting of feedback parameters, the close-loop bandwidth, limited by the low pass filter bandwidth in the Lock-in amplifier, can be extended far beyond the open-loop bandwidth. The experimental data show good agreement with the simulation results.

关键词： frequency response close loop Bell-Bloom magnetometer

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Engine-Map-Based Predictive Fuel-Efficient Control Strategies for a Group of Connected Vehicles

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Automotive Innovation 2018年第4期1卷 311-319页

作者： Lihong Qiu Lijun Qian Zoleikha Abdollahi Zhouwei Kong Pierluigi Pisu Changan Intelligent Vehicle R&D Institute Chongqing 401120China Department of Vehicle Engineering Hefei University of TechnologyHefei 230009China International Center for Automotive Research Clemson UniversityGreenville 29607USA

An engine-map-based predictive fuel-efficient control strategy for a group of connected vehicles is presented. A decentralizedmodel predictive control framework is formulated to predict the optimal velocity profile that compromises fuel economy andmobility while guaranteeing the safety of each vehicle. In the model predictive control framework, an engine-map-based fuelconsumption model is established by implementing a backward conventional vehicle model in the cost function. Moreover,the cost function is normalized by dividing each term by its reference value. An extra cost is added to the safety term when thedistance between adjacent vehicles drops to a critical value to guarantee vehicle safety, while another extra cost is consideredfor the velocity tracking term to prevent the violation of traffic rules. The results of simulation show the effectiveness of theproposed control method.

关键词： Model predictive control Connected vehicles Fuel-efficient control Engine map Intelligent transportation system

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Microstructure and Wear Resistance of 62Cu-38Zn Brass with Bionic Coupling Units Treated by Laser Cladding 4

Microstructure and Wear Resistance of 62Cu-38Zn Brass with B...

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4th International Conference on Applied Materials and Manufacturing technology (ICAMMT)

作者： Hu Yong Cao Yong Hu Kaifeng Hu Ye Li Ling Shen Minghao Zhang Zhihui Jilin Univ Sch Mech Sci & Engn Changchun 130022 Jilin Peoples R China CRRC Zhuzhou Locomot Co Ltd Zhuzhou 412001 Peoples R China Jilin Univ Coll Automot Engn Changchun 130022 Jilin Peoples R China Jilin Univ Minist Educ Key Lab Bion Engn Changchun 130022 Jilin Peoples R China

In order to improve the wear resistance of 62Cu-38Zn brass, bionic coupling units were fabricated by laser cladding using Ni-based, Fe-based and Co-based self-fluxing alloy powders. The microstructures, chemical composition and mechanical properties of the units were studied in this paper. Wear resistance of samples was examined by dry sliding wear. Good metallurgical bonding between the unit and substrate was obtained, and fine dendritic microstructure was resulted in the unit zone, which proved marked availability of laser cladding on brass. Laser cladding treatments with different self-fluxing alloy powders were beneficial to reinforcing the surface of brass and then improved its wear resistance with various results. The Ni-based alloy powders led to the best wear resistance amongst the all samples.

关键词： Wear resistance

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Automatic Liver Segmentation on CT Images 3rd

Automatic Liver Segmentation on CT Images

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3rd Conference on Signal and Information Processing - Networking and Computers (ICSINC)

作者： Celik, Torecan Song, Hong Chen, Lei Yang, Jian Beijing Inst Technol Sch Software Beijing Peoples R China Beijing Inst Technol Sch Opt & Elect Beijing Peoples R China

ISBN: (纸本)9789811075216;9789811075209

In this paper, a new coarse-to-fine framework is proposed for automatic liver segmentation on abdominal computed tomography (CT) images. The framework consists of two steps including rough segmentation and refined segmentation. The rough segmentation is implemented based on histogram thresholding and the largest connected component algorithm. Firstly, gray value range of the liver is obtained from image histogram, then the liver area is extracted from the rest of an image according to the largest connected component algorithm. The refined segmentation is performed based on the improved GrowCut (IGC) algorithm, which generates the label seeds automatically. The experimental results show that the proposed framework can efficiently segment the liver on CT images.

关键词： Liver segmentation CT images Improved Grow-Cut histogram thresholding

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Replication-Based Fault-Tolerance for Large-Scale Graph Processing

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IEEE TRANSACTIONS ON PARALLEL AND DISTRIBUTED SYSTEMS 2018年第7期29卷 1621-1635页

作者： Chen, Rong Yao, Youyang Wang, Peng Zhang, Kaiyuan Wang, Zhaoguo Guan, Haibing Zang, Binyu Chen, Haibo Shanghai Jiao Tong Univ Shanghai Key Lab Scalable Comp & Syst Shanghai 200240 Peoples R China NYU Syst Grp 550 1St Ave New York NY 10003 USA

The increasing algorithmic complexity and dataset sizes necessitate the use of networked machines for many graph-parallel algorithms, which also makes fault tolerance a must due to the increasing scale of machines. Unfortunately, existing large-scale graph-parallel systems usually adopt a distributed checkpoint mechanism for fault tolerance, which incurs not only notable performance overhead but also lengthy recovery time. This paper observes that the vertex replicas created for distributed graph computation can be naturally extended for fast in-memory recovery of graph states. This paper describes Imitator, a new fault tolerance mechanism, which supports cheap maintenance of vertex states by replicating them to their replicas during normal message exchanges, and provides fast in-memory reconstruction of failed vertices from replicas in other machines. Imitator has been implemented on Cyclops with edge-cut and PowerLyra with vertex-cut. Evaluation on a 50-node EC-2 like cluster shows that Imitator incurs an average of 1.37 and 2.32 percent performance overhead (ranging from -0.6 to 3.7 percent) for Cyclops and PowerLyra respectively, and can recover from failures of more than one million of vertices with less than 3.4 seconds.

关键词： Graph-parallel system fault-tolerance replication

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Bacterial Genome Editing via a Designed Toxin-Antitoxin Cassette

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ACS SYNTHETIC BIOLOGY 2018年第3期7卷 822-831页

作者： Wu, Jie Deng, Aihua Sun, Qinyun Bai, Hua Sun, Zhaopeng Shang, Xiuling Zhang, Yun Liu, Qan Liang, Yong Liu, Shuwen Che, Yongsheng Wen, Tingyi Chinese Acad Sci Inst Microbiol CAS Key Lab Pathogen Microbiol & Immunol Beijing 100101 Peoples R China Beijing Inst Pharmacol & Toxicol State Key Lab Toxicol & Med Countermeasures Beijing 100850 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Univ Chinese Acad Sci Savaid Med Sch Beijing 100049 Peoples R China

Manipulating the bacterial genomes in an efficient manner is essential to biological and biotechnological research. Here, we reprogrammed the bacterial TA systems as the toxin counter-selectable cassette regulated by an antitoxin switch (TCCRAS) for genetic modifications in the extensively studied, and utilized Gram-positive bacteria, B. subtilis and Corynebacterium glutamicum. In the five characterized type II TA systems, the ReIBE complex can specifically and efficiently regulate cell growth and death by the conditionally controlled antitoxin ReIB switch, thereby serving as a novel counter selectable cassette to establish the TCCRAS system. Using a single vector, such a system has been employed to perform in frame deletion, functional knock-in, gene replacement, precise point mutation, large-scale insertion, and especially, deletion of the fragments up to 194.9 kb in B. subtilis. In addition, the biosynthesis of lycopene was first achieved in B. subtilis using TCCRAS to integrate a 5.4-kb fusion cluster(P-spc-crtI-crtE-crtB). The system was further adapted for gene knockdown and replacement, and large-scale deletion of the fragments up to 179.8 kb in C. glutamicum, with the mutation efficiencies increased by 0.8-1.0-fold compared to the conventional SacB method. TCCRAS thus holds promise as an effective and versatile genome-scale engineering technology for metabolic engineering and synthetic genomics research in a broad range of the Gram-positive bacteria.

关键词： toxin-antitoxin (TA) system genome editing large-scale deletion Bacillus subtilis Corynebacterium glutamicum

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PhoPepMass： A database and search tool assisting human phosphorylation peptide identification from mass spectrometry data

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Journal of Genetics and Genomics 2018年第7期45卷 381-388页

作者： Menghuan Zhang Hui Cui Lanming Chen Ying Yu Michael O. Glocker Lu Xie School of Life Science and Technology Shanghai Tech University Shanghai 201210 China Shanghai Center for Bioinformation Technology Shanghai Academy of Science and Technology Shanghai 201203 China Institute for Nutritional Sciences Shanghai Institutes for Biological Sciences University of Chinese Academy of Sciences Shanghai 200031 China College of Food Science and Technology Shanghai Ocean University Shanghai 201306 China Department of Pharmacology School of Basic Medical Sciences Tianjin Medical University Tianjin 30007 China Proteome Center Rostock University of Rostock Rostoek 18055 Germany

Protein phosphorylation, one of the most important protein post-translational modifications, is involved in various biological processes, and the identification of phosphorylation peptides （phosphopeptides） and their corresponding phosphorylation sites （phosphosites） will facilitate the understanding of the molecular mechanism and function of phosphorylation. Mass spectrometry （MS） provides a high- throughput technology that enables the identification of large numbers of phosphosites. PhoPepMass is designed to assist human phosphopeptide identification from MS data based on a specific database of phophopeptide masses and a multivariate hypergeometric matching algorithm. It contains 244,915 phosphosites from several public sources. Moreover, the accurate masses of peptides and fragments with phosphosites were calculated. It is the first database that provides a systematic resource for the query of phosphosites on peptides and their corresponding masses. This allows researchers to search certain proteins of which phosphosites have been reported, to browse detailed phosphopeptide and fragment information, to match masses from MS analyses with defined threshold to the corresponding phos- phopeptide, and to compare proprietary phosphopeptide discovery results with results from previous studies. Additionally, a database search software is created and a ＂two-stage search strategy＂ is suggested to identify phosphopeptides from tandem mass spectra of proteomics data. We expect PhoPepMass to be a useful tool and a source of reference for proteomics researchers.

关键词： Phosphosite Phosphopeptide Phosphopeptide mass

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