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Bacteriocin production by lactic acid bacteria isolated from Rioja red wines

JOURNAL OF APPLIED MICROBIOLOGY 2000年第1期88卷 44-51页

作者： Navarro, L Zarazaga, M Sáenz, J Ruiz-Larrea, F Torres, C Univ La Rioja Dept Agr & Alimentac Logrono 26004 Spain

Forty-two lactic acid bacteria (LAB) of the genera Lactobacillus (32), Leuconostoc (6), Pediococcus (3) and Lactococcus (1), isolated from Rioja red wines, were tested for antimicrobial activity. All these strains, as well as 18 Leuconostoc oenos and 19 yeast strains were used as indicators. Only nine strains showed antimicrobial activity, and all were of the species Lactobacillus plantarum, which constitutes the predominant microflora in Rioja red wines after alcoholic fermentation. Lact. plantarum strain J-51 showed the widest range of action, inhibiting the growth of 31 strains of the four studied LAB genera. Lact. plantarum J-51 antimicrobial activity was lost after treatment with proteases, suggesting a proteinaceous nature for this activity. It was found to be stable between pH 3 and 9 and under strong heating conditions (100 degrees C for 60 min). Polymerase chain reaction (PCR) analysis of Lact. plantarum J-51 genome revealed the presence of the plnA gene that encodes the plantaricin precursor PlnA. A 366-bp fragment was sequenced and showed 95% identity with pln locus of Lact. plantarum C-11. The deduced precursor peptide sequence showed one mutation (Gly7 to Ser7) at the double glycine leader peptide, and the three putative 26-, 23- and 22-residue active peptides remain identical to those of Lact. plantarum C-11. Therefore, antimicrobial peptides constitute a potent adaptation advantage for those strains that dominate in a medium such as wine, and can play an important role in the ecology of wine microflora.

关键词：氨基酸序列细菌素类/生物合成细菌素类/遗传学乳杆菌属/生长和发育乳杆菌属/代谢乳球菌属/生长和发育乳球菌属/代谢明串珠菌属/生长和发育明串珠菌属/代谢微生物敏感性试验分子序列数据片球菌属/生长和发育片球菌属/代谢聚合酶链反应蛋白质前体/遗传学序列分析 DNA 序列分析蛋白质链球菌科/生长和发育链球菌科/代谢葡萄酒/微生物学

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Effect of some nutritional and environmental parameters on the production of diacetyl and on starch consumption by Pediococcus pentosaceus and Lactobacillus acidophilus in submerged cultures

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JOURNAL OF APPLIED MICROBIOLOGY 2000年第1期88卷 142-153页

作者： Escamilla, ML Valdés, SE Soriano, J Tomasini, A Univ Autonoma Metropolitana Iztapalapa Dept Biotecnol Mexico City 09340 DF Mexico Univ Nacl Autonoma Mexico Fac Estudios Super Cuautitlan Cuautitlan 04510 Mexico

Three series of 5-day submerged cultures with Pediococcus pentosaceus MITJ-10 and Lactobacillus acidophilus Hansen 1748 were carried out in starch-based media, and the effect of cultural factors on the changes of starch, diacetyl and amylase activity determined. In axenic cultures, Ped. pentosaceus MITJ-10 produced more diacetyl (63.27 mg l(-1)) by adding glucose, yeast extract and CaCO3 (P < 0.01), at 28 degrees C (P < 0.05);but more starch was consumed (18.4 g l(-1)) in the absence of glucose (P < 0.01). Lact. acidophilus Hansen 1748 consumed more starch (26.56 g l(-1)) at 28 degrees C, with CaCO3, glucose (P < 0.01) and yeast extract (P < 0.05);however, the amylolytic activity (10 077 U l(-1)) was favoured at 35 degrees C (P < 0.01). Little starch was consumed in mixed cultures due to the low pH;nevertheless, diacetyl content rose to 135.76 mg l(-1) at 32 degrees C (P < 0.01). Therefore, both studied strains might be useful to produce aromatic extensors from starchy substrates. These natural aromatic extensors are of interest to the food industry.

关键词：淀粉酶类/代谢细菌学技术碳酸钙/药理学色谱法气相培养基二乙酰/代谢嗜酸乳杆菌/生长和发育嗜酸乳杆菌/代谢片球菌属/生长和发育片球菌属/代谢淀粉/代谢温度

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Lactobacillus farciminis MD, a newer strain with potential for bacteriocin and antibiotic assay

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LETTERS IN APPLIED MICROBIOLOGY 2000年第3期30卷 197-202页

作者： Halami, PM Chandrashekar, A Nand, K Cent Food Technol Res Inst Dept Food Microbiol Mysore 570013 Karnataka India

A native isolate Lactobacillus farciminis MD isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. Also, the efficacy of Lact. farciminis MD as a sensitive strain for antibiotic assay was established against different antibiotics including ampicillin, cefazoline, chloramphenicol and nitrofurantoin at concentrations of 30 mu g each, showing an inhibition zone of 30 mm diameter. The high degree of sensitivity towards bacteriocins and antibiotics provide potential for the exploitation of Lact. farciminis MD in establishing very well-defined bacteriocin producers.

关键词：伞菌目抗菌药/药理学细菌素类/生物合成细菌素类/药理学发酵食品微生物学乳杆菌属/药物作用乳杆菌属/遗传学乳杆菌属/分离和提纯明串珠菌属/代谢微生物敏感性试验片球菌属/代谢

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Characterization and identification of Pediococcus species isolated from forage crops and their application for silage preparation

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APPLIED AND ENVIRONMENTAL MICROBIOLOGY 1999年第7期65卷 2901-2906页

作者： Cai, YM Kumai, S Ogawa, M Benno, Y Nakase, T Inst Phys & Chem Res Japan Collect Microorganisms Wako Saitama 3510198 Japan Ehime Univ Coll Agr Matsuyama Ehime 7908566 Japan Kyushu Natl Agr Expt Stn Kumamoto 8611192 Japan

Pediococcus species isolated from forage crops were characterized, and their application to silage preparation was studied. Most isolates were distributed on forage crops at low frequency. These isolates could be divided into three (A, B, and C) groups by their sugar fermentation patterns. Strains LA 3, LA 35, and LS 5 are representative isolates from groups A, B, and C, respectively. Strains LA 3 and LA 35 had intragroup DNA homology values above 93.6%, shelving that they belong to the species Pediococcus acidilactici. Strain LS 5 belonged to Pediococcus pentosaceus on the basis of DNA-DNA relatedness. All three of these strains and strain SL 1 (Lactobacillus casei, isolated from a commercial inoculant) were used as additives to alfalfa and Italian ryegrass silage preparation at two temperatures (25 and 48 degrees C). When stored at 25 degrees C, all of the inoculated silages were well preserved and exhibited significantly (P < 0.05) reduced fermentation losses compared to that of their control in alfalfa and Italian ryegrass silages. When stored at 48 degrees C, silages inoculated with strains LA 3 and LA 35 were also well preserved, with a significantly (P < 0.05) lower pH, butyric acid and ammonia-nitrogen content, gas production, and dry matter loss and significantly (P < 0.05) higher lactate content than the control, but silages inoculated with LS 5 and SL 1 were of poor quality. P. acidilactici LA 3 and LA 35 are considered suitable as potential silage inoculants.

关键词：集落计数微生物 DNA 细菌/化学 DNA 细菌/遗传学 DNA 核糖体/化学 DNA 核糖体/遗传学发酵紫苜蓿/微生物学分子序列数据核酸杂交片球菌属/分类片球菌属/遗传学片球菌属/分离和提纯片球菌属/代谢种系发生禾本科/微生物学 RNA 核糖体 16S/遗传学青贮饲料/微生物学玉米/微生物学

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Cloning and expression of the pediocin operon in Streptococcus thermophilus and other lactic fermentation bacteria

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CURRENT MICROBIOLOGY 1999年第5期39卷 295-301页

作者： Coderre, PE Somkuti, GA USDA ARS Eastern Reg Res Ctr Wyndmoor PA 19038 USA

Production of pediocin in Pediococcus acidilactici is associated with pMBR 1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors that contain all four structural genes, the chromosomal promoter STP2201 from Streptococcus thermophilus, and repA from the 2-kbp S. thermophilus plasmid pER8. The recombinant plasmid, pPC318, expressed and secreted active pediocin in Escherichia coli. Streptococcus thermophilus, Lactococcus lactis subsp. lactis, and Enterococcus faecalis::were electrotransformed with pPC418, a modified vector fitted with an erythromycin resistance tracking gene. Pediocin was produced and secreted in each of the lactic acid bacteria, and production was Stable for up to ten passages. The expression of pediocin in dairy fermentation microbes has important implications for bacteriocins as food preservatives in dairy products.

关键词：细菌素类/生物合成细菌素类/遗传学细菌素类/药理学克隆分子肠球菌粪/遗传学肠球菌粪/代谢大肠杆菌/遗传学大肠杆菌/代谢乳杆菌属/药物作用乳球菌乳/遗传学乳球菌乳/代谢微生物敏感性试验操纵子片球菌属/遗传学片球菌属/代谢质粒/遗传学链球菌属/药物作用链球菌属/遗传学链球菌属/代谢

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