检索结果-内蒙古大学图书馆

Treatment of Vicia faba root tip cells with specific inhibitors to cyclin-dependent kinases leads to abnormal spindle formation

引用

PLANT JOURNAL 1998年第6期16卷 697-707页

作者： binarová, P Dolezel, J Draber, P Heberle-bors, E Strnad, M bögre, L Univ Vienna Vienna Bioctr Inst Microbiol & Genet A-1030 Vienna Austria De Montfort Univ Norman Borlaug Ctr Plant Sci Inst Expt Bot Prague 14220 Czech Republic Acad Sci Czech Republ Inst Microbiol CR-14220 Prague Czech Republic De Montfort Univ Norman Borlaug Ctr Plant Sci Inst Expt Bot ASCR Olomouc Czech Republic ASCR Inst Mol Genet Prague 14220 Czech Republic Palacky Univ Inst Expt Bot Lab Growth Regulators Olomouc 78371 Czech Republic

Many events during cell division are triggered by an evolutionary conserved regulator the cyclin-dependent kinase (Cdk). Here we used two novel drugs, the purine analogues bohemine and roscovitine, to study the role of Cdks in cell cycle progression and microtubule organisation in Vicia faba root tip cells. both drugs inhibited the activity of immunopurified Vicia faba and alfalfa Cdc2-kinase. The transcript levels of an A and b type cyclin, as well as of the cdc2 genes, declined in treated root tips, white the mRNA level of a D-type cyclin gene was not affected. An observed transient arrest at the G1/S and G2/M regulatory points indicated that inhibition of the Cdc2-kinase had an effect on both transitions. In contrast to the regular bipolar spindle in untreated cell, in drug-treated metaphase cells abnormally short and dense kinetochore microtubule fibres were observed. These microtubules were randomly arranged in the vicinity of the kinetochores and connected the chromosomes. Thus, the chromosomes were not aligned on the metaphase plate but were arranged in a circle, with kinetochores pointing inwards and chromosome arms pointing outwards, gamma-Tubulin, which plays a role in microtubule nucleation, also localised to the centre of the monopolar spindle. The observed abnormalities in mitosis, after inhibition of Cdc2-kinase by specific drugs, suggest a role for this enzyme in regulating some of the steps leading to a bipolar spindle structure.

关键词： CDC2蛋白激酶/拮抗剂和抑制剂 CDC2蛋白激酶/遗传学细胞周期/药物作用细胞周期蛋白A/遗传学细胞周期蛋白b/遗传学酶抑制剂/药理学豆科/细胞学分生组织/细胞学分生组织/药物作用分生组织/超微结构微管/药物作用微管/超微结构有丝分裂纺锤体/药物作用植物根/细胞学植物根/药物作用植物根/超微结构植物药用蛋白激酶类/代谢嘌呤类/药理学转录遗传

来源：评论

学校读者我要写书评

暂无评论

The mitogen-activated protein kinase signaling pathway stimulates Mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation

引用

MOLECULAR AND CELLULAR bIOLOGY 1999年第3期19卷 1990-1999页

作者： Howard, EL Charlesworth, A Welk, J MacNicol, AM Univ Chicago Dept Med Chicago IL 60637 USA Univ Chicago Comm Dev Biol Chicago IL 60637 USA Univ Chicago Comm Canc Biol Chicago IL 60637 USA

The Mos protein kinase is a key regulator of vertebrate oocyte maturation. Oocyte-specific Mos protein expression is subject to translational control. In the frog Xenopus, the translation of Mos protein requires the progesterone-induced polyadenylation of the maternal Mos mRNA, which is present in the oocyte cytoplasm. both the Xenopus p42 mitogen-activated protein kinase (MAPK) and maturation-promoting factor (MPF) signaling pathways have been proposed to mediate progesterone-stimulated oocyte maturation. In this study, we have determined the relative contributions of the MAPK and MPF signaling pathways to Mos mRNA polyadenylation. We report that progesterone-induced Mos mRNA polyadenylation was attenuated in oocytes expressing the MAPK phosphatase rVH6. Moreover, inhibition of MAPK signaling blocked progesterone-induced Mos protein accumulation. Activation of the MAPK pathway by injection of RNA encoding Mos was sufficient to induce both the polyadenylation of synthetic Mos mRNA substrates and the accumulation of endogenous Mos protein in the absence of MPF signaling. Activation of MPF, by injection of cyclin bI RNA or purified cyclin b1 protein, also induced both Mos protein accumulation and Mos mRNA polyadenylation, However, this action of MPF required MAPK activity. by contrast, the cytoplasmic polyadenylation of maternal cyclin b1 mRNA nas stimulated by MPF in a MAPK-independent manner, thus revealing a differential regulation of maternal mRNA polyadenylation by the MAPK;and MPF signaling pathways. We propose that MAPK-stimulated Mos mRNA cytoplasmic polyadenylation is a key component of the positive-feedback loop, which contributes to the all-or-none process of oocyte maturation.

关键词：钙-钙调素依赖性蛋白激酶类/代谢细胞周期蛋白b/遗传学细胞周期蛋白b1 细胞质成熟促进因子/代谢卵母细胞/生理学聚腺苷酸类蛋白质生物合成原癌基因蛋白质c-mos/遗传学 RNA 信使信号传导爪蟾属动物

来源：评论

学校读者我要写书评

暂无评论

Conservation and function of a potential substrate-binding domain in the yeast Clb5 b-type cyclin

引用

MOLECULAR AND CELLULAR bIOLOGY 2000年第13期20卷 4782-4790页

作者： Cross, FR Jacobson, MD Rockefeller Univ New York NY 10021 USA

Cyclin A contains a region implicated in binding to the p27 inhibitor and to substrates. There is strong evolutionary conservation of surface residues contributing to this region in many cyclins, including yeast b-type cyclins, despite the absence of a yeast p27 homolog. The yeast S-phase b-type cyclin Clb5p interacted with mammalian p27 in a two-hybrid assay. This interaction was disrupted by mutations designed to disrupt hydrophobic interactions (hpm mutation) or hydrogen bonding (Q241A mutation) based on the cyclin A-p27 crystal structure. In contrast, mutation of the Clb5p p27-binding domain only slightly reduced binding and inhibition by the Sic1p Clb-Cdc28p kinase inhibitor. Mutations disrupting the p27-binding domain strongly reduced Clb5p biological activity in diverse assays without reducing Clb5p-associated kinase activity. An analogous hpm mutation in the mitotic cyclin Clb2p reduced mitotic function, but in some assays this mutation increased the ability of Clb2p to perform functions normally restricted to Clb5p. These results support the idea of a modular, structurally conserved cyclin domain involved in substrate targeting.

关键词：氨基酸序列结合部位细胞周期蛋白质类保守序列细胞周期蛋白b/遗传学细胞周期蛋白b/代谢周期素依赖激酶抑制蛋白质类周期素依赖激酶抑制剂p21 周期素依赖激酶抑制剂p27 细胞周期蛋白类/代谢进化分子真菌蛋白质类/遗传学真菌蛋白质类/代谢微管相关蛋白质类/代谢突变酿酒酵母蛋白质类底物特异性肿瘤抑制蛋白质类酵母菌/遗传学酵母菌/代谢

来源：评论

学校读者我要写书评

暂无评论

Human transcription factor hTAFb>IIb>150 (CIF150) is involved in transcriptional regulation of cell cycle progression

引用

MOLECULAR AND CELLULAR bIOLOGY 1999年第8期19卷 5548-5556页

作者： Martin, J Halenbeck, R Kaufmann, J Chiron Corp Chiron Technol Emeryville CA 94608 USA

Here we present evidence that CIF150 (hTAF(II)150), the human homolog: of Drosophila TAF(II)150, plays an important and selective role in establishing gene expression patterns necessary for progression through the cell cycle. Gel filtration experiments demonstrate that CIF150 (hTAF(II)150) seems to be less tightly associated with human transcription factor IID than hTAF(II)130 is associated with hTAF(II)250. The transient functional knockout of CIF150 (hTAF(II)150) protein led to cell cycle arrest at the G(2)/M transition in mammalian cell lines. PCR display analysis with the RNA derived from CIF150-depleted cells indicated that CIF150 (hTAF(II)150) is required for the transcription of only a subset of RNA polymerase II genes. CIF150 (hTAF(II)150) directly stimulated cyclin b1 and cyclin ii transcription in cotransfection assays and in vitro assays, suggesting that the expression of these genes is dependent on CIF150 (hTAF(II)150) function. We defined a CIF150 (hTAF(II)150) consensus binding site and demonstrated that a CIF150-responsive cis element is present in the cyclin b1 core promoter. These results suggest that one function of CIF150 (hTAF(II)150) is to select specific RNA polymerase II core promoter elements involved in cell cycle progression.

关键词：细胞周期/遗传学细胞周期/生理学细胞系色谱法凝胶细胞周期蛋白A/生物合成细胞周期蛋白A/遗传学细胞周期蛋白b/生物合成细胞周期蛋白b/遗传学细胞周期蛋白b1 DNA结合蛋白质类/生理学果蝇蛋白质类 G2期基因表达调控基因表达调控肿瘤基因打靶基因报告 HeLa细胞大分子物质中期肿瘤蛋白质类/生物合成肿瘤蛋白质类/遗传学 RNA聚合酶Ⅱ/代谢逆转录聚合酶链反应底物特异性 TATA结合蛋白质相关因子类转录因子TFIID 转录因子/生理学转录因子 TFⅡ类/代谢转录遗传/生理学转染人类

来源：评论

学校读者我要写书评

暂无评论

Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitment

引用

MOLECULAR AND CELLULAR bIOLOGY 2000年第22期20卷 8397-8408页

作者： Grandin, N Damon, C Charbonneau, M Ecole Normale Super Lyon ENS CNRS UMR 5665 F-69364 Lyon France

The Saccharomyces cerevisiae CDC13 protein binds single-strand telomeric DNA. Here we report the isolation of new mutant alleles of CDC13 that confer either abnormal telomere lengthening or telomere shortening. This deregulation not only depended on telomerase (Est2/TLC1) and Est1, a direct regulator of telomerase, but also on the yeast Ku proteins, yKu70/Hdf1 and yKu80/Hdf2, that have been previously implicated in DNA repair and telomere maintenance. Expression of a Cdc13-yKu70 fusion protein resulted in telomere elongation, similar to that produced by a Cdc13-Est1 fusion, thus suggesting that yKu70 might promote Cdc13-mediated telomerase recruitment. We also demonstrate that Stn1 is an inhibitor of telomerase recruitment by Cdc13, based both on STN1 overexpression and Cdc13-Stn1 fusion experiments. We propose that accurate regulation of telomerase recruitment by Cdc13 results from a coordinated balance between positive control by yKu70 and negative control by Stn1. Our results represent the first evidence of a direct control of the telomerase-loading function of Cdc13 by a double-strand telomeric DNA-binding complex.

关键词：抗原核染色体蛋白质类非组蛋白/遗传学染色体蛋白质类非组蛋白/代谢细胞周期蛋白b/遗传学细胞周期蛋白b/代谢 DNA解旋酶类 DNA结合蛋白质类/遗传学 DNA结合蛋白质类/代谢真菌蛋白质类/遗传学真菌蛋白质类/代谢基因表达调控真菌细胞内信号肽和蛋白质类突变核蛋白质类/遗传学核蛋白质类/代谢蛋白质丝氨酸苏氨酸激酶重组融合蛋白质类/遗传学重组融合蛋白质类/代谢酿酒酵母菌/遗传学酿酒酵母菌/代谢酿酒酵母蛋白质类序列分析 DNA 末端转移酶端粒/遗传学末端转移酶端粒/代谢

来源：评论

学校读者我要写书评

暂无评论

Genetic alterations of the retinoblastoma-related gene Rb2/p130 identify different pathogenetic mechanisms in and among burkitt's lymphoma subtypes

引用

AMERICAN JOURNAL OF PATHOLOGY 2000年第3期156卷 751-760页

作者： Cinti, C Leoncini, L Nyongo, A Ferrari, F Lazzi, S bellan, C Vatti, R Zamparelli, A Cevenini, G Tosi, GM Claudio, PP Maraldi, NM Tosi, P Giordano, A Univ Siena Inst Pathol Anat & Histol I-53100 Siena Italy CNR Inst Normal & Pathol Cytomorphol Bologna Italy Nairobi Hosp Dept Pathol Nairobi Kenya Univ Siena Inst Biomed Technol I-53100 Siena Italy Thomas Jefferson Univ Jefferson Med Coll Dept Pathol Anat & Cell Biol Philadelphia PA 19107 USA Univ Naples Federico II Dept Sci Odontostomatol & Maxillofacciali Naples Italy IOR Lab Cell Biol & Electron Microscopy Bologna Italy

Alterations of cell cycle-associated genes probably contribute to the pathogenesis of burkitt's Lymphoma (bL), in addition to c-myc translocation, Mutations disrupting the nuclear localization signal of the retinoblastoma-related gene Rb2/p130 have been documented recently in bL cell lines and primary tumors. Given the importance of the Rb2/p130 gene in controlling cell growth, mutations of this gene may result in uncontrolled cell proliferation. We tested the expression and genomic organization of the Rb2/p130 gene in relation to the proliferative features of a series of bL samples collected from the endemic and sporadic regions, regardless of whether the samples were acquired immune deficiency syndrome (AIDS)-related. The expression of the Rb2/p130, p107, and cell proliferation-related proteins (cyclin A and b) was determined by immunohistochemistry. The structures of exons 19 through 22 of the Rb2/p130 gene, encoding for the b domain and C terminus, were analyzed by polymerase chain reaction (PCR) analysis and single-strand conformation polymorphism (SSCP) technique. The direct PCR products were sequenced to identify the actual mutations. Our results suggest that bL is composed of a mixture of molecular types with distinct genetic and phenotypic patterns, probably resulting from different pathogenetic mechanisms. In endemic bL, the Rb2/p130 gene is mutated in most of the cases, and the protein is restricted to the cytoplasm, In AIDS-related bL, high levels of nuclear expression of the wild-type pRb2/p130, p107, and cell proliferation-related proteins were detected. This finding is in Line with the molecular mechanisms observed in virus-linked oncogenesis. Sporadic bLs were mainly characterized by the low nuclear values of the wild-type pRb2/p130 and, conversely, the high values of p107, The increased cell proliferation due to different alterations of cell growth control by Rb-related proteins may be the first step in lymphomagenesis, during which additional genet

关键词：细胞凋亡伯基特淋巴瘤/分类伯基特淋巴瘤/遗传学伯基特淋巴瘤/代谢伯基特淋巴瘤/病理学细胞周期蛋白A/遗传学细胞周期蛋白A/代谢细胞周期蛋白b/遗传学细胞周期蛋白b/代谢 DNA引物/化学 DNA 肿瘤/分析 DNA 病毒/分析免疫表型分型原位杂交淋巴瘤艾滋病相关/遗传学淋巴瘤艾滋病相关/代谢淋巴瘤艾滋病相关/病理学有丝分裂指数核蛋白质类/遗传学核蛋白质类/代谢磷蛋白类/遗传学磷蛋白类/代谢点突变聚合酶链反应多态现象单链构象蛋白质类视网膜母细胞瘤蛋白质/遗传学视网膜母细胞瘤蛋白质/代谢视网膜母细胞瘤样蛋白质p107 视网膜母细胞瘤样蛋白质p130 青少年成年人儿童儿童学龄前女(雌)性人类男(雄)性中年人

来源：评论

学校读者我要写书评

暂无评论

Translational control of cyclin b1 mRNA during meiotic maturation: Coordinated repression and cytoplasmic polyadenylation

引用

DEVELOPMENTAL bIOLOGY 2000年第1期220卷 97-109页

作者： barkoff, AF Dickson, KS Gray, NK Wickens, N Univ Wisconsin Dept Biochem Madison WI 53706 USA

Translational control is prominent during meiotic maturation and early development. In this report, we investigate a mode of translational repression in Xenopus laevis oocytes, focusing on the mRNA encoding cyclin b1. Translation of cyclin b1 mRNA is relatively inactive in the oocyte and increases dramatically during meiotic maturation. We show, by injection of synthetic mRNAs, that the cis-acting sequences responsible for repression of cyclin b1 mRNA reside within its 3'UTR. Repression can be saturated by increasing the concentration of reporter mRNA injected, suggesting that the cyclin b1 3'UTR sequences provide a binding site for a trans-acting repressor. The sequences that direct repression overlap and include cytoplasmic polyadenylation elements (CPEs), sequences known to promote cytoplasmic polyadenylation. However, the presence of a CPE per se appears insufficient to cause repression, as other mRNAs that contain CPEs are not translationally repressed. We demonstrate that relief of repression and cytoplasmic polyadenylation are intimately linked. Repressing elements do not override the stimulatory effect of a long poly(A) tail, and polyadenylation of cyclin b1 mRNA is required for its translational recruitment. Our results suggest that translational recruitment of endogenous cyclin b1 mRNA is a collaborative effect of derepression and poly(A) addition. We discuss several molecular mechanisms that might underlie this collaboration. (C) 2000 Academic Press.

关键词： 3'非翻译区碱基序列细胞周期蛋白b/遗传学细胞质/代谢基因表达调控发育期萤光素酶类/遗传学减数分裂分子序列数据卵母细胞/生长和发育卵母细胞/代谢质粒/遗传学聚腺苷酸类/代谢蛋白质生物合成 RNA 信使/遗传学 RNA 信使/代谢爪蟾光滑动物女(雌)性

来源：评论

学校读者我要写书评

暂无评论

Testing cyclin specificity in the exit from mitosis

引用

MOLECULAR AND CELLULAR bIOLOGY 2000年第13期20卷 4483-4493页

作者： Jacobson, MD Gray, S Yuste-Rojas, M Cross, FR Rockefeller Univ New York NY 10021 USA Boston Consulting Grp New York NY USA Pharma Mar Madrid Spain

Cyclical inactivation of b-type cyclins has been proposed to be required for alternating DNA replication and mitosis, Destruction box-dependent Clb5p degradation is strongly increased in mitotic cells, and constitutive overexpression of Clb5p lacking the destruction box resulted in rapid accumulation of inviable cells, frequently multiply budded, with DNA contents ranging from unreplicated to apparently fully replicated. Loss of viability correlated with retention of nuclear Clb5p at the time of nuclear division. CLb2-Delta db overexpression that was quantitatively comparable to CLb5-Delta db overexpression with respect to Clb protein production and Clb-associated kinase activity resulted in a distinct phenotype: reversible mitotic arrest with uniformly replicated DNA. Simultaneous overexpression of CLb2-Delta db and CLb5-Delta db overexpressers similarly resulted in a uniform arrest with replicated DNA, and this arrest was significantly more reversible than that observed with CLb5-Delta db overexpression alone. These results suggest that Clb2p and not Clb5p can efficiently block mitotic completion. We speculate that CLb5-Delta db overexpression may be lethal, because persistence of high nuclear Clb5p-associated kinase throughout mitosis leads to failure to load origins of replication, thus preventing DNA replication in the succeeding cell cycle.

关键词：细胞周期/生理学细胞核/代谢细胞周期蛋白b/遗传学细胞周期蛋白b/代谢真菌蛋白质类/遗传学真菌蛋白质类/代谢基因表达调控真菌基因致死有丝分裂突变重组蛋白质类/遗传学重组蛋白质类/代谢酿酒酵母蛋白质类酵母菌/细胞学酵母菌/遗传学酵母菌/代谢

来源：评论

学校读者我要写书评

暂无评论

Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth

引用

NATURE 2000年第6791期406卷 90-94页

作者： Zhu, GF Spellman, PT Volpe, T brown, PO botstein, D Davis, TN Futcher, b Cold Spring Harbor Lab Cold Spring Harbor NY 11724 USA Univ Washington Dept Biochem Seattle WA 98195 USA Stanford Univ Med Ctr Dept Genet Stanford CA 94306 USA SUNY Stony Brook Grad Program Genet Stony Brook NY 11794 USA Stanford Univ Med Ctr Dept Biochem Stanford CA 94306 USA

There are about 800 genes in Saccharomyces cerevisiae whose transcription is cell-cycle regulated(1,2). Some of these form clusters of co-regulated genes(1). The 'CLb2' cluster contains 33 genes whose transcription peaks early in mitosis, including CLb1, CLb2, SWI5, ACE2, CDC5, CDC20 and other genes important for mitosis(1). Here we rnd that the genes in this cluster lose their cell cycle regulation in a mutant that lacks two forkhead transcription factors, Fkh1 and Fkh2. Fkh2 protein is associated with the promoters of CLb2, SWI5 and other genes of the cluster. These results indicate that Fkh proteins are transcription factors for the CLb2 cluster. The fkh1 fkh2 mutant also displays aberrant regulation of the 'SIC1' cluster(1), whose member genes are expressed in the M-G1 interval and are involved in mitotic exit. This aberrant regulation may be due to aberrant expression of the transcription factors Swi5 and Ace2, which are members of the CLb2 cluster and controllers of the SIC1 cluster. Thus, a cascade of transcription factors operates late in the cell cycle. Finally, the fkh1 fkh2 mutant displays a constitutive pseudohyphal morphology, indicating that Fkh1 and Fkh2 may help control the switch to this mode of growth.

关键词：结合部位细胞周期蛋白b/遗传学 DNA 真菌/代谢叉头转录因子类基因表达调控真菌基因真菌基因 cdc 多基因族突变核蛋白质类/遗传学寡核苷酸序列分析沉淀素试验启动区(遗传学) 蛋白质结合酵母菌酿酒/遗传学酵母菌酿酒/生长和发育酿酒酵母蛋白质类转录因子/遗传学

来源：评论

学校读者我要写书评

暂无评论

The tumour suppressor protein p53 can repress transcription of cyclin b

引用

NUCLEIC ACIDS RESEARCH 2000年第22期28卷 4410-4418页

作者： Krause, K Wasner, M Reinhard, W Haugwitz, U Dohna, CLZ Mössner, J Engeland, K Univ Leipzig Max Burger Forschungszentrum Med Klin 2 D-04103 Leipzig Germany

The tumour Suppressor protein p53 has functions in controlling the G(1)/S and G(2)/M transitions. Central regulators for progression from G(2) to mitosis are b-type cyclins complexed with cdc2 kinase, In mammals two cyclin b proteins are found, cyclin b1 and b2, We show that upon treatment of HepG2 cells with 5-fluorouracil or methotrexate, p53 levels increase while concentrations of cyclin b2 mRNA, measured by RT-PCR with the LightCycler system, are reduced, In DLD-1 colorectal adenocarcinoma cells (DLD-1-tet-off-p53) cyclin bland,b2 mRNA levels drop after expression of wild-type p53 but not after induction of a DNA binding-deficient mutant of p53, Analysis of the cyclin: b2 promoter reveals specific repression of this gene by p53, Transfection of wild-type p53 into SaOS-2 Cells shuts off transcription from a cyclin b2 promoter-luciferase construct whereas a p53 mutant protein does not, The cyclin b2 promoter does not contain a consensus p53 binding site, Most of the p53-dependent transcriptional responsiveness resides in its 226 bp core promoter, Taken together with earlier:observations on p53-dependent transcription of cyclin bI, our results suggest that one way Of-regulating G(2) arrest may be a reduction in cyclin b levels through p53-dependent transcriptional repression.

关键词：细胞周期蛋白b/遗传学细胞周期蛋白b1 细胞周期蛋白b2 氟尿嘧啶/药理学基因表达调控肿瘤/药物作用萤光素酶类/遗传学萤光素酶类/代谢甲氨蝶呤/药理学突变启动区遗传/遗传学 RNA 信使/遗传学 RNA 信使/代谢重组融合蛋白质类/遗传学重组融合蛋白质类/代谢转录遗传肿瘤细胞培养的肿瘤抑制蛋白质p53/遗传学肿瘤抑制蛋白质p53/生理学人类

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：