检索结果-内蒙古大学图书馆

神经生物学

引用

中国学术期刊文摘 2006年第11期12卷 7页

Nurr1基因与干细胞多巴胺能表型分化的研究进展;肉毒神经毒素受体的研究进展。[编者按]

关键词：肉毒毒素类/代谢神经毒素类/代谢神经生物学

来源：评论

学校读者我要写书评

暂无评论

High-throughput fluorogenic assay for determination of botulinum type B neurotoxin protease activity

引用

ANALYTICAL BIOCHEMISTRY 2001年第2期291卷 253-261页

作者： Anne, C Cornille, F Lenoir, C Roques, BP UFR Sci Pharmaceut & Biol INSERM U266 CNRSUMR 8600Dept Pharmacochim Mol & Struct F-75270 Paris 06 France

Botulinum neurotoxins are responsible for botulism, a flaccid muscular paralysis caused by inhibition of acetylcholine release at the neuromuscular junction, This occurs by cleavage of conserved proteins involved in exocytosis such as synaptobrevin by the zinc metallopeptidase activity of the light chain of some botulinum neurotoxins. Botulism, for which there is presently no therapy available, is a relatively widespread disease that may result in death. Consequently, the development of drugs able to inhibit the hydrolytic activity of these neurotoxins is of great interest, Design and screening of such inhibitors could be largely facilitated by using high-throughput assays, With this aim, a novel in vitro test for quantifying the proteolytic activity of botulinum type B neurotoxin was developed. The substrate is the 60-94 fragment of human synaptobrevin-1 which was modified by introduction of the fluorescent amino acid L-pyrenylalanine in position 74 and a p-nitrophenylalanyl residue as quenching group in position 77, The cleavage of Syb 60-94 [Pya(74), Nop(77)] by th, toxin active chain occurs selectively between residues 76 and 77 as in the case of the unmodified synaptobrevin and is directly quantified by measuring the strong fluorescence of the formed metabolite Syb 60-76 [Pya(74)], This is the easiest, quickest, and cheapest assay described to date for measuring the proteolytic activity of botulinum type B neurotoxin, It can be easily automated for high-throughput screening, Moreover, amounts of about 3.5 pg/ml of botulinum type B neurotoxin could be detected by this method, (C) 2001 Academic Press.

关键词：生物学鉴定法/方法肉毒毒素类/代谢校准色谱法高压液相荧光染料/代谢动力学膜蛋白质类/代谢金属内肽酶类/代谢 R-SNARE蛋白质类分光光度法人类

来源：评论

学校读者我要写书评

暂无评论

Binding of Clostridium botulinum C2 toxin to asparagine-linked complex and hybrid carbohydrates

引用

JOURNAL OF BIOLOGICAL CHEMISTRY 2000年第4期275卷 2328-2334页

作者： Eckhardt, M Barth, H Blöcker, D Aktories, K Univ Freiburg Inst Pharmakol & Toxikol D-79104 Freiburg Germany

Clostridium botulinum C2 toxin is a binary toxin composed of an enzymatic subunit (C2I) capable of ADP-ribosylating actin and a binding subunit (C2II) that is responsible for interaction with receptors on eukaryotic cells. Here we show that binding of C2 toxin depends on the presence of asparagine-linked carbohydrates. A recently identified Chinese hamster ovary cell mutant (Fritz G., Schroeder, P., and Aktories, K. (1995) Infect. Immun. 63, 2334-2340) was found to be deficient in N-acetylglucosaminyltransferase I. C2 sensitivity of this mutant was restored by transfection of an N-acetylglucosaminyltransferase I cDNA C2 toxin sensitivity was reduced after inhibition of alpha-mannosidase II. In contrast, Chinese hamster ovary cell mutants deficient in sialylated (Lec2) or galactosylated (Lec8) glycoconjugates showed an increase in toxin sensitivity compared with wild-type cells. Our results show that the GlcNAc residue linked beta-1,2 to the alpha-1,3-mannose of the asparagine-linked core structure is essential for C2II binding to Chinese hamster ovary cells.

关键词：天冬酰胺酶/代谢碱基序列肉毒毒素类/代谢 CHO细胞仓鼠亚科 DNA引物甘露糖苷酶类/拮抗剂和抑制剂 N-乙酰氨基葡糖转移酶类/遗传学 N-乙酰氨基葡糖转移酶类/代谢多糖类/代谢蛋白质结合重组蛋白质类/遗传学重组蛋白质类/代谢 α甘露糖苷酶动物

来源：评论

学校读者我要写书评

暂无评论

Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B

引用

NATURE STRUCTURAL BIOLOGY 2000年第8期7卷 693-699页

作者： Swaminathan, S Eswaramoorthy, S Brookhaven Natl Lab Dept Biol Upton NY 11973 USA

Clostridium botulinum neurotoxins are among the most potent toxins to humans. The crystal structures of intact C. botulinum neurotoxin type B (BoNT/B) and its complex with sialyllactose, determined at 1.8 and 2.6 Angstrom resolution, respectively, provide insight into its catalytic and binding sites. The position of the belt region in BoNT/B is different from that in BoNT/A;this observation presents interesting possibilities for designing specific inhibitors that could be used to block the activity of this neurotoxin. The structures of BoNT/B and its complex with sialyllactose provide a detailed description of the active site and a model for interactions between the toxin and its cell surface receptor. The latter may provide valuable information for recombinant vaccine development.

关键词：氨基酸序列结合部位肉毒毒素类/化学肉毒毒素类/代谢肉毒杆菌毒素类 A型/化学肉毒杆菌毒素类 A型/代谢催化域肉毒梭菌/化学结晶学 X线神经节苷脂类/代谢乳糖/化学乳糖/代谢模型分子分子序列数据蛋白质结构二级蛋白质结构三级受体细胞表面/化学受体细胞表面/代谢序列比对唾液酸类/化学唾液酸类/代谢

来源：评论

学校读者我要写书评

暂无评论

Intimate details of the most poisonous poison

引用

NATURE STRUCTURAL BIOLOGY 2000年第8期7卷 617-619页

作者： Singh, BR Univ Massachusetts Dept Chem & Biochem Dartmouth MA 02747 USA

Crystal structures of type B botulinum neurotoxin, determined under conditions relevant to the extracellular binding and intracellular substrate cleavage steps of its toxic action, reveal that minimal structural changes occur upon binding a ganglioside or after substrate cleavage. The unique structure of this toxin provides information for developing preventive measures against botulism.

关键词：生物转运肉毒毒素类/化学肉毒毒素类/代谢细胞膜/代谢肉毒梭菌/化学结晶学 X线神经节苷脂类/代谢膜蛋白质类/代谢模型分子蛋白质结合蛋白质构象 SNARE蛋白质类膜泡运输蛋白质类

来源：评论

学校读者我要写书评

暂无评论

Preliminary crystallographic studies of a protease-resistant botulinum neurotoxin associated protein Hn-33

引用

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 1999年第6期55卷 1237-1239页

作者： Hadfield, AT Petsko, G Lindo, P Singh, BR Brandeis Univ Rosenstiel Basic Med Sci Res Ctr Dept Biochem Waltham MA 02254 USA Univ Manchester Dept Chem & Biochem N Dartmouth MA 02747 USA

Botulinum neurotoxin (BoNT) is one of the most potent toxins known. BoNT is also a food poison, which means that the toxin must survive the protease action and acidity of the gut. A group of neurotoxin-associated proteins which are only beginning to be identified and characterized are believed to be responsible for this protection. Hn-33 is a 33 kDa polypeptide which is a major component of the type A botulinum neurotoxin complex. Crystals of Hn-33 have been grown by vapour-diffusion techniques. They belong to a primitive orthorhombic space group and diffract to a resolution of 2.6 Angstrom, with unit-cell parameters a = 130.3, b = 122.2, c = 37.2 Angstrom.

关键词：细菌蛋白质类肉毒毒素类/化学肉毒毒素类/代谢结晶学 X线内肽酶类/代谢血凝素类/化学血凝素类/代谢蛋白质构象

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：