检索结果-内蒙古大学图书馆

The MAZ protein is an autoantigen of Hodgkin's disease and paraneoplastic cerebellar dysfunction

ANNALS OF NEUROLOGY 2003年第1期53卷 123-127页

作者： Bataller, L Wade, DF Graus, F Rosenfeld, MR Dalmau, J Univ Arkansas Med Sci Dept Neurol Little Rock AR USA Univ Arkansas Med Sci Dept Microbiol & Immunol Little Rock AR USA Hosp Clin Barcelona Dept Neurol Barcelona Spain Cent Arkansas Vet Hlth Care Syst Little Rock AR USA

Probing a cerebellar expression library with TrAb sera from patients with Hodgkin's disease and paraneoplastic cerebellar degeneration resulted in the isolation of MAZ (myc-associated zinc-finger protein). Eleven of 19 TrAb sera and 16 of 131 controls reacted with MAZ, indicating a significant, although not specific, association between Tr and MAZ immunities (p < 0.001). Interestingly, 9 of 16 positive control patients also had cerebellar dysfunction. Purified MAZ antibodies reacted with Purkinje cells. In neuronal cells, MAZ interacts with DCC (Deleted in Colorectal Cancer product), the receptor for netrin-1, a neuronal survival factor. These findings suggest epitope spreading between the Tr antigen and the MAZ-DCC complex and offer a possible model of immune-mediated cerebellar disease.

关键词：自身抗体/血液自身抗原/遗传学自身抗原/免疫学细胞黏附分子/代谢 DNA结合蛋白质类表位基因文库霍奇金病/免疫学副肿瘤性小脑变性/免疫学浦肯野细胞/免疫学受体细胞表面转录因子/遗传学转录因子/免疫学转录因子/代谢肿瘤抑制蛋白质类/代谢人类

来源：评论

学校读者我要写书评

暂无评论

Peroxisome proliferator-activated receptor-γ agonists prevent experimental autoimmune encephalomyelitis

引用

ANNALS OF NEUROLOGY 2002年第6期51卷 694-702页

作者： Feinstein, DL Galea, E Gavrilyuk, V Brosnan, CF Whitacre, CC Dumitrescu-Ozimek, L Landreth, GE Pershadsingh, HA Weinberg, G Heneka, MT Univ Illinois Dept Anesthesiol Chicago IL 60612 USA Albert Einstein Coll Med Dept Pathol Bronx NY 10467 USA Ohio State Univ Dept Mol Virol Immunol & Med Genet Columbus OH 43210 USA Univ Bonn Dept Neurol D-5300 Bonn Germany Case Western Reserve Univ Dept Neurosci Cleveland OH 44106 USA Case Western Reserve Univ Dept Neurol Cleveland OH 44106 USA Univ Calif Irvine Irvine CA USA Kern Med Ctr Dept Family Med Irvine CA USA

The development of clinical symptoms in multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE) involves T-cell activation and migration into the central nervous system, production of glial-derived inflammatory molecules, and demyelination and axonal damage. Ligands of the peroxisome proliferator-activated receptor (PPAR) exert anti-inflammatory effects on glial cells, reduce proliferation and activation of T cells, and induce myelin gene expression. We demonstrate in two models of EAE that orally administered PPAR-gamma ligand pioglitazone reduced the incidence and severity of monophasic, chronic disease in C57BL/6 mice immunized with myelin oligodendrocyte glycoprotein peptide and of relapsing disease in B10.P1 mice immunized with myelin basic protein. Pioglitazone also reduced clinical signs when it was provided after disease onset. Clinical symptoms were reduced by two other PPAR-gamma agonists, suggesting a role for PPARgamma activation in protective effects. The suppression of clinical signs was paralleled by decreased lymphocyte infiltration, lessened demyelination, reduced chemokine and cytokine expression, and increased inhibitor of kappa B (IkB) expression in the brain. Pioglitazone also reduced the antigen-dependent interferon-gamma production from EAE-derived T cells. These results suggest that orally administered PPARgamma agonists could provide therapeutic benefit in demyelinating disease.

关键词：小脑/细胞学小脑/生理学大脑皮质/细胞学大脑皮质/生理学 DNA结合蛋白质类/遗传学 DNA结合蛋白质类/代谢脑脊髓炎自身免疫性实验性/药物疗法脑脊髓炎自身免疫性实验性/免疫学脑脊髓炎自身免疫性实验性/病理学糖蛋白类/投药和剂量糖蛋白类/免疫学降血糖药/治疗应用 I-κB蛋白质类配体淋巴细胞活化小鼠近交C57BL 多发性硬化/免疫学多发性硬化/病理生理学一氧化氮合酶/遗传学一氧化氮合酶/代谢一氧化氮合酶Ⅱ型恶唑类/治疗应用肽碎片/投药和剂量肽碎片/免疫学受体胞质和核/激动剂受体胞质和核/免疫学缓解诱导脊髓/细胞学脊髓/病理学 T淋巴细胞/免疫学 T淋巴细胞/代谢噻唑类/治疗应用噻唑烷二酮类转录因子/激动剂转录因子/免疫学酪氨酸/类似物和衍生物酪氨酸/治疗应用动物女(雌)性人类男(雄)性小鼠

来源：评论

学校读者我要写书评

暂无评论

Opposing roles of Elk-1 and its brain-specific isoform, short Elk-1, in nerve growth factor-induced PC12 differentiation

引用

JOURNAL OF BIOLOGICAL CHEMISTRY 2001年第7期276卷 5189-5196页

作者： Vanhoutte, P Nissen, JL Brugg, B Della Gaspera, B Besson, MJ Hipskind, RA Caboche, J Univ Paris 06 Inst Neurosci Lab Neurochim Anat CNRSUMR 7624 F-75005 Paris France Univ Paris 06 Inst Neurosci Lab Dev & Vieillissement SNC CNRSUMR 7624 F-75005 Paris France Univ Paris 06 Inst Neurosci Mol Neurogenet Lab CNRSUMR 7624 F-75005 Paris France CNRS IFR 24 UMR 5535 Inst Genet Mol F-34293 Montpellier 5 France

The ternary complex factor Elk-1, a major nuclear target of extracellular signal-regulated kinases, is a strong transactivator of serum-responsive element (SRE) driven gene expression. We report here that mature brain neurons and nerve growth factor (NGF)-differentiated PC12 cells also express a second, smaller isoform of Elk-1, short Elk-1 (sElk-1). sElk-1 arises from an internal translation start site in the Elk-1 sequence, which generates a protein lacking the first 54 amino acids of the DNA-binding domain. This deletion severely compromises the ability of sElk-1 to form complexes with serum response factor on the SRE in vitro and to activate SRE reporter genes in the presence of activated Ras. Instead, sElk, but not a mutant that cannot be phosphorylated, inhibits transactivation driven by Elk-1. More pertinent to the neuronal-specific expression of sElk-1, we show it plays an opposite role to Elk-1 in potentiating NGF-driven PC12 neuronal differentiation. Overexpression of sElk-1 but not Elk-1 increases neurite extension, an effect critically linked to its phosphorylation. interestingly, in the presence of sElk-1, Elk-1 loses its strictly nuclear localization to resemble the nuclear/cytoplasm pattern observed in the mature brain. This is blocked by mutating a normally cryptic nuclear export signal in Elk-1. These data provide new insights into molecular events underlying neuronal differentiation of PC12 cells mediated by the NGF-ERK signaling cascade.

关键词：主动转运细胞核抗体/免疫学脑/代谢细胞分化细胞核/代谢密码子起始 DNA结合蛋白质类/遗传学 DNA结合蛋白质类/免疫学 DNA结合蛋白质类/生理学神经生长因子/药理学神经元/细胞学神经元/代谢 PC12细胞表型蛋白质亚型/遗传学蛋白质亚型/免疫学蛋白质亚型/生理学原癌基因蛋白质类/遗传学原癌基因蛋白质类/免疫学原癌基因蛋白质类/生理学大鼠 Sprague-Dawley 反式激活(遗传学) 转录因子/遗传学转录因子/免疫学转录因子/生理学 ets结构域蛋白Elk1 动物男(雄)性大鼠

来源：评论

学校读者我要写书评

暂无评论

Selective elimination of leukemic CD34⁺ progenitor cells by cytotoxic T lymphocytes specific for WT1

引用

BLOOD 2000年第7期95卷 2198-2203页

作者： Gao, LQ Bellantuono, I Elsässer, A Marley, SB Gordon, MY Goldman, JM Stauss, HJ Hammersmith Hosp Imperial Sch Med Dept Immunol London England Hammersmith Hosp Imperial Sch Med Dept Hematol London England

Hematologic malignancies such as acute and chronic myeloid leukemia are characterized by the malignant transformation of immature CD34(+) progenitor cells. Transformation is associated with elevated expression of the Wilm's tumor gene encoded transcription factor (WT1), Here we demonstrate that WT1 can serve as a target for cytotoxic T lymphocytes (CTL) with exquisite specificity for leukemic progenitor cells. HLA-A0201-restricted CTL specific for WT1 kill leukemia cell lines and inhibit colony formation by transformed CD34(+) progenitor cells isolated from patients with chronic myeloid leukemia (CML), whereas colony formation by normal CD34(+) progenitor cells is unaffected. Thus, the tissue-specific transcription factor WT1 is an ideal target for CTL-mediated purging of leukemic progenitor cells in vitro and for antigen-specific therapy of leukemia and other WT1-expressing malignancies in vivo. (Blood. 2000;95:2198-2203) (C) 2000 by The American Society of Hematology.

关键词：抗原/免疫学抗原 CD34/分析印迹法蛋白质骨髓净化/方法 DNA结合蛋白质类/分析 DNA结合蛋白质类/免疫学基因肾母细胞瘤造血干细胞/化学造血干细胞/免疫学造血干细胞/病理学 T淋巴细胞细胞毒性/免疫学转录因子/分析转录因子/免疫学肿瘤细胞培养的 WT1蛋白质类成年人儿童人类男(雄)性

来源：评论

学校读者我要写书评

暂无评论

HMG-I/Y, a new c-Myc target gene and potential oncogene

引用

MOLECULAR AND CELLULAR BIOLOGY 2000年第15期20卷 5490-5502页

作者： Wood, LJ Mukherjee, M Dolde, CE Xu, Y Maher, JF Bunton, TE Williams, JB Resar, LMS Johns Hopkins Univ Sch Med Div Hematol Baltimore MD 21205 USA Johns Hopkins Univ Sch Med Dept Pediat Baltimore MD 21205 USA Johns Hopkins Univ Sch Med Dept Mol Biol & Genet Baltimore MD 21205 USA Johns Hopkins Univ Sch Med Dept Comparat Med Baltimore MD 21205 USA Johns Hopkins Univ Sch Med Dept Oncol Baltimore MD 21205 USA

The HMG-I/Y gene encodes the HMG-I and HMG-Y proteins, which function as architectural chromatin binding proteins important in the transcriptional regulation of several genes. Although increased expression of the HMG-I/Y proteins is associated with cellular proliferation, neoplastic transformation, and several human cancers, the role of these proteins in the pathogenesis of malignancy remains unclear. To better understand the role of these proteins in cell growth and transformation, we have been studying the regulation and function of HMG-I/Y. The HMG-I/Y promoter was cloned, sequenced, and subjected to mutagenesis analysis. A c-Myc-Max consensus DNA binding site was identified as an element important in the serum stimulation of HMG-I/Y. The oncoprotein c-Myc and its protein partner Max bind to this site in vitro and activate transcription in transfection experiments. HMG-I/Y expression is stimulated by c-Myc in a Myc-estradiol receptor cell line in the presence of the protein synthesis inhibitor cycloheximide, indicating that HMG-I/Y is a direct c-Myc target gene. HMG-I/Y induction is decreased in Myc-deficient fibroblasts. HMG-I/Y protein expression is also increased in Burkitt's lymphoma cell lines, which are known to have increased c-Myc protein. Like Myc, increased expression of HMG-I protein leads to the neoplastic transformation of both Rat la fibroblasts and CB33 cells. In addition, Rat la cells overexpressing HMG-I protein form tumors in nude mice. Decreasing HMG-I/Y proteins using an antisense construct abrogates transformation in Burkitt's lymphoma cells. These findings indicate that HMG-I/Y is a c-Myc target gene involved in neoplastic transformation and a member of a new class of potential oncogenes.

关键词：碱性螺旋-环-螺旋亮氨酸拉链转录因子类碱性亮氨酸拉链转录因子类伯基特淋巴瘤细胞系细胞转化肿瘤/遗传学 DNA结合蛋白质类/遗传学 DNA结合蛋白质类/代谢成纤维细胞/药物作用成纤维细胞/病理学基因表达调控生长物质/遗传学生长物质/代谢生长物质/药理学 HMGA1a蛋白高迁移率族蛋白质类/遗传学高迁移率族蛋白质类/免疫学高迁移率族蛋白质类/代谢小鼠近交BALBC 小鼠裸肿瘤蛋白质类/药物作用肿瘤蛋白质类/遗传学肿瘤蛋白质类/代谢启动区遗传原癌基因蛋白质c-myc/遗传学原癌基因蛋白质c-myc/代谢重组蛋白质类/遗传学重组蛋白质类/代谢序列分析 DNA 转录因子/遗传学转录因子/免疫学转录因子/代谢转录遗传肿瘤细胞培养的动物小鼠大鼠

来源：评论

学校读者我要写书评

暂无评论

Do lampreys have lymphocytes? The Spi evidence

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2000年第13期97卷 7417-7422页

作者： Shintani, S Terzic, J Sato, A Saraga-Babic, M O'hUigin, C Tichy, H Klein, J Max Planck Inst Biol Immungenet Abt D-7076 Tubingen Germany Univ Split Sch Med Dept Physiol Split Croatia Univ Split Sch Med Dept Histol & Embryol Split Croatia

It is generally accepted that living jawless vertebrates (lampreys and hagfishes) lack the capability of mounting an adaptive immune response. At the same time, however, there are reports describing histological evidence for the presence in agnathan tissues of lymphocytes, the key players in adaptive immunity. The question therefore arises whether the cells identified morphologically as lymphocytes are true lymphocytes in terms of their genetic: developmental program. In this study, evidence is provided that the lampreys express a member of the purine box 1 (PU.1)/spleen focus-forming virus integration B (Spi-B) gene family known to be critically and specifically involved in the differentiation of lymphocytes in jawed vertebrates. The lamprey gene is expressed in the lymphocyte-like cells of the digestive tract and inexplicably also in the ovary.

关键词：氨基酸序列碱基序列细胞分化 DNA结合蛋白质类/遗传学 DNA结合蛋白质类/免疫学免疫七鳃鳗/免疫学淋巴细胞/细胞学淋巴细胞/免疫学分子序列数据序列比对转录因子/遗传学转录因子/免疫学动物

来源：评论

学校读者我要写书评

暂无评论

Human slug is a repressor that localizes to sites of active transcription

引用

MOLECULAR AND CELLULAR BIOLOGY 2000年第14期20卷 5087-5095页

作者： Hemavathy, K Guru, SC Harris, J Chen, JD Ip, YT Univ Massachusetts Sch Med Dept Cell Biol Program Mol Med Worcester MA 01605 USA Univ Massachusetts Sch Med Dept Biochem & Mol Biol Worcester MA 01605 USA Univ Massachusetts Sch Med Dept Mol Pharmacol & Toxicol Worcester MA 01605 USA Natl Human Genome Res Inst Genet & Mol Biol Branch NIH Bethesda MD 20892 USA

Snail/Slug family proteins have been identified in diverse species of both vertebrates and invertebrates. The proteins contain four to six zinc fingers and function as DNA-binding transcriptional regulators. Various members of the family have been demonstrated to regulate cell movement, neural cell fate, left-right asymmetry, cell cycle, and apoptosis. However, the molecular mechanisms of how these regulators function and the target genes involved are largely unknown. In this report, we demonstrate that human Slug (hSlug) is a repressor and modulates both activator-dependent and basal transcription. The repression depends on the C-terminal DNA-binding zinc fingers and on a separable repression domain located in the N terminus. This domain may recruit histone deacetylases to modify the chromatin and effect repression. Protein localization study demonstrates that hSlug is present in discrete foci in the nucleus. This subnuclear pattern does not colocalize with the PML foci or the coiled bodies. Instead, the hSlug foci overlap extensively with areas of the SC-35 staining, some of which have been suggested to be sites of active splicing or transcription. These results lead us to postulate that hSlug localizes to target promoters, where activation occurs, to repress basal and activator-mediated transcription.

关键词：碱基序列结合部位细胞核/遗传学细胞核/代谢染色质/代谢保守序列 DNA结合蛋白质类/代谢酶抑制剂/药理学荧光抗体技术组蛋白脱乙酰基酶抑制剂组蛋白脱乙酰基酶类/代谢羟肟酸类/药理学分子序列数据肿瘤蛋白质类/免疫学肿瘤蛋白质类/代谢核蛋白质类 RNA剪接阻遏蛋白质类/遗传学阻遏蛋白质类/免疫学阻遏蛋白质类/代谢序列缺失转录因子/遗传学转录因子/免疫学转录因子/代谢转录遗传肿瘤抑制蛋白质类锌指人类

来源：评论

学校读者我要写书评

暂无评论

Signaling via LAT (linker for T-cell activation) and Syk/ZAP70 is required for ERK activation and NFAT transcriptional activation following CD2 stimulation

引用

BLOOD 2000年第6期96卷 2181-2190页

作者： Martelli, MP Lin, HM Zhang, WQ Samelson, LE Bierer, BE NHLBI Lab Lymphocyte Biol NIH Bethesda MD 20892 USA NCI Cellular & Mol Biol Lab NIH Bethesda MD 20892 USA

Activation of T cells can be initiated through cell surface molecules in addition to the T-cell receptor-CD3 (TCR-CD3) complex. In human T cells, ligation of the CD2 molecule by mitogenic pairs of anti-CD2 monoclonal antibodies activates T cells via biochemical signaling pathways similar but not identical to those elicited on TCR engagement. This study describes a key role for the p36/38 membrane adapter protein linker for T cell activation (LAT) in CD2-mediated T cell activation. Following ligation of CD2 on the surface of the Jurkat T-cell line and human purified T cells, LAT was tyrosine phosphorylated and shown to associate in vivo with a number of other tyrosine phosphorylated proteins including PLC gamma-1, Grb-2, and SLP-76. Using Jurkat cell lines deficient in ZAP70/Syk (P116) or LAT (ANJ3) expression, CD2-dependent PLC gamma-1 and SLP-76 tyrosine phosphorylation required expression both of ZAP70 or Syk and of LAT. As predicted, the absence of either LAT or ZAP70/Syk kinases correlated with a defect in the induction of nuclear factor of activated T cells (NFAT) transcriptional activity, activation of the interleukin-2 promoter, and ERK phosphorylation following CD2 stimulation. These data suggest that LAT is an adapter protein important for the regulation of CD2-mediated T-cell activation. (Blood. 2000;96:2181-2190) (C) 2000 by The American Society of Hematology.

关键词：衔接蛋白质类信号转导抗原 CD2/免疫学载体蛋白质类/免疫学 DNA结合蛋白质类/免疫学酶前体/免疫学细胞内信号肽和蛋白质类 Jurkat细胞淋巴细胞活化膜蛋白质类丝裂原激活蛋白激酶类/免疫学 NFATC转录因子类核蛋白质类磷蛋白类/免疫学蛋白酪氨酸激酶类/免疫学信号传导/免疫学 T淋巴细胞/免疫学转录因子/免疫学 ZAP-70蛋白酪氨酸激酶人类

来源：评论

学校读者我要写书评

暂无评论

Role of Drosophila IKKγ in a Toll-independent antibacterial immune response

引用

NATURE IMMUNOLOGY 2000年第4期1卷 342-347页

作者： Rutschmann, S Jung, AC Zhou, R Silverman, N Hoffmann, JA Ferrandon, D CNRS Inst Biol Mol & Cellulaire UPR 9022 F-67084 Strasbourg France Harvard Univ Dept Mol & Cellular Biol Cambridge MA 02138 USA

We have generated, by ethylmethane sulfonate mutagenesis, loss-of-function mutants in the Drosophila homolog of the mammalian I-kappa B kinase (IKK) complex component IKK gamma (also called NEMO). Our data show that Drosophila IKK gamma is required for the Relish-dependent immune induction of the genes encoding antibacterial peptides and for resistance to infections by Escherichia coli. However, it is not required for the Toll-Dip-dependent antifungal host: defense,The results indicate distinct control mechanisms of the Rel-like transactivators DIF and Relish in the Drosophila innate immune response and show that Drosophila Toll does not signal through a IKK gamma-dependent signaling complex. Thus, in contrast to the vertebrate inflammatory response, IKK gamma is required for the activation of only one immune signaling pathway in Drosophila.

关键词：抗原细菌/免疫学果蝇属/免疫学果蝇蛋白质类基因表达调控/免疫学 I-κB激酶免疫先天昆虫蛋白质类/免疫学膜糖蛋白类/免疫学蛋白质丝氨酸苏氨酸激酶/免疫学受体细胞表面信号传导/免疫学 Toll样受体转录因子/遗传学转录因子/免疫学动物

来源：评论

学校读者我要写书评

暂无评论

XIST RNA associates with specific regions of the inactive X chromatin

引用

JOURNAL OF BIOLOGICAL CHEMISTRY 2000年第47期275卷 36491-36494页

作者： Gilbert, SL Pehrson, JR Sharp, PA MIT Ctr Canc Res Dept Biol Cambridge MA 02139 USA Univ Penn Sch Vet Med Dept Biol Anim Philadelphia PA 19104 USA

Microscopy studies have shown that XIST RNA colocalizes with the inactive X chromosome (Xi). However, the molecular basis for this colocalization is unknown, Here we provide two lines of evidence from chromatin immunoprecipitation experiments that XTST RNA physically associates with the Xi chromatin. First, XTST RNA can be co-precipitated by antiserum against macroH2A, a histone H2A variant enriched in the Xi. Second, XIST RNA can be co-precipitated by antisera that recognize unacetylated, but not acetylated, isoforms of histones H3 and H4. The specificity of XIST RNA association with hypoacetylated chromatin, together with the previous finding that hypoacetylated histone H4 is enriched at promoters of X-inactivated genes, raises the possibility that XIST RNA may contribute to the hypoacetylation of specific regions of the Xi so as to alter the expression of X-linked genes.

关键词：抗体特异性细胞培养的染色质/化学剂量补偿作用遗传学纤连蛋白类/遗传学组蛋白类/遗传学免疫血清启动区(遗传学) RNA 未翻译/遗传学 RNA 未翻译/免疫学转录因子/遗传学转录因子/免疫学 X染色体动物小鼠

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：