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THE DIffERENCES IN PLASMA-PROTEIN PATTERN BETWEEN LAYING AND NON-LAYING CHICKENS, QUAILS AND GEESE

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 1985年第2期80卷 309-313页

作者： KURYL, J GASPARSKA, J Polish Academy of Sciences Institute of Genetics and Animal Breeding Jastrzȩbiec 05-551 Mroków Poland

The pattern of proteins in blood plasma of chicken, quail and goose was studied in relation to egg laying. The method of horizontal polyacrylamide gradient gel electrophoresis with a 12% separation gel and a discontinuous buffer system (Tris-citrate-borate pH 9.0) was used. Considerable differences in the protein patterns between laying and non-laying birds in 4 regions of plasma proteins: prealbumin, pretransferrin, post-transferrin and .gamma.-globulin were noticed.

关键词： f three regions of pre-albumin M three regions of pre-albumin S three regions of pre-albumin Alb albumin Lt β-livetin α 1 α 1 -globulin Prt pretransferrin Tf transferrin Ptf post-transferrin γ-glob γ-globulin

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MEMBRANE-SPECIfIC INHIBITORS Of THE BOVINE HEART MITOCHONDRIAL ATPASE

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BIOCHIMICA ET BIOPHYSICA ACTA 1985年第2期806卷 277-282页

作者： fELDMAN, RI SIGMAN, DS UNIV CALIF LOS ANGELES SCH MED INST MOLEC BIOL LOS ANGELES CA 90024 USA UNIV CALIF LOS ANGELES SCH MED DEPT BIOL CHEM LOS ANGELES CA 90024 USA

New cationic inhibitors of the bovine heart mitochondrial ATPase were synthesized by quaternizing 1-dansylamido-3-dimethypropylamine with decyl and hexadecyl iodides. These ligands are unique in their mode of action because they inhibit the submitochondrial membrane-associated forms of the enzyme more potently than the soluble form of the enzyme (f1). Derivatives prepared with propyl or hexyl iodides were weak inhibitors and exhibit little affinity for submitochondrial membranes particle. The inhibitory effectiveness of these derivatives measured either in the direction of ATP synthesis or ATP hydrolysis results from efficient insertion into the membrane. Other inhibitory organic cations such as the 3:1 4,7-diphenyl-1,10-phenanthroline-ferrous chelate and alkyl guanidines inhibit both the membrane-associated and soluble ATPase comparably.

关键词： f 1 -ATPase Inhibitor-membrane interaction Synthetic inhibitor (Bovine heart mitochondria) fCCP, carbonyl cyanide p -trifluoromethoxyphenylhydrazone ETPH, submitochondrial particles

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INTERACTION Of MYELIN BASIC-PROTEIN WITH GANGLIOSIDES AND GANGLIOSIDE-PHOSPHOLIPID MIXTURES

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BIOCHIMICA ET BIOPHYSICA ACTA 1985年第2期819卷 225-230页

作者： BACH, D SELA, B WEIZMANN INST SCI DEPT MEMBRANE RES IL-76100 REHOVOT ISRAEL TEL AVIV UNIV DEPT BIOCHEM IL-69978 TEL AVIV ISRAEL

The interaction of myelin basic protein with monosialoganglioside GM1 was investigated. It was found that the emission maximum of the tryptophan of the protein is blue-shifted due to the interaction. In mixtures of the monosialoganglioside with phosphatidylcholine, the myelin basic protein induces phase separation of the lipids as inferred from differential scanning calorimetry experiments.

关键词： Myelin basic protein Ganglioside fluorescence Differential scanning calorimetry PC phosphatidylcholine PS phosphatidylserine GM 1 galactosyl- N -acetylgalactosaminyl( N -acetylneuraminyl)galactosylglucosylceramide DSC differential scanning calorimetry f fluorescence

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DETERMINATION Of MEMBRANE-POTENTIAL WITH LIPOPHILIC CATIONS - CORRECTION Of PROBE BINDING

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BIOCHIMICA ET BIOPHYSICA ACTA 1985年第2期820卷 207-215页

作者： DEMURA, M KAMO, N KOBATAKE, Y Department of Biophysics Faculty of Pharmaceutical Sciences Hokkaido University Sapporo Japan

The binding of lipophilic ions to the membrane of envelope vesicles from Halobacterium halobium was examined in the absence and presence of membrane potential. The lipophilic ions used constitute a homologous series of (Phe)3-P+-(CH2)n-Ch3 (n = 0-4) and tetraphenylphosphonium (TPP+). In the absence of membrane potential, the amounts of binding were proportional to the probe concentration in the medium when the concentration is dilute. Upon illumination, interior negative membrane potential is generated which induces the uptake of phosphonium cation probe. 2 .mu.M were employed as the initial probe concentration. The real membrane potential was evaluted by means of extrapolation of the state of no binding: The values of Ciapp/Co for various probes are plotted against the binding coefficient. Here, Ciapp is the apparent intra-vesicular concentration of the probes which is calculated without consideration of bound probes. The ordinate intercept of the plot gives the true concentration ratio, and from this the membrane potential is evaluated. The membrane is split into two halves, outer and inner half, and the amounts of bound probes in each region are governed by the concentration in the contiguous solution. We obtained a formula which describes amounts of binding as a function of the membrane potential.

关键词： Ion-selective electrode Phosphonium cation Membrane potential Lipophilic cation binding Envelope vesicles ( H. halobium ) C r the probe concentration of r region ( r = o, mo, mi and i) C i app intravesicular concentration of probe which is calculated with neglection of bound probes f K mo V mo (K mo V mo + K mi V mi ) Hepes-(2-hydroxyethyl)-1-piperazineethanesulfonic acid i intra-vesicular aqueuous space K b binding coefficient (= K mo V mo + K mi V mi ) K mo k 1 k 2 in the absence of membrane potential K mi k 6 k 5 in the absence of membrane potential k r rate constants (see fig. 1) mi inner half of membrane mo outer half of membrane n the fraction of the membrane potential that imposes effectively on mo region (Eqn. 6) o extra-vesicular space TPMP + triphenylmethyl phosphonium TPEP + triphenylethyl phosphonium TPPP + triphenylpropyl phosphonium TPBP + triphenylbutyl phosphonium TPAP + triphenylamyl phosphonium TPP + tetraphenyl phosphonium U , uptake of probe by vesicles (= V i C i app ) U b (0) amounts of binding of probe in the absence of Δϕ U b (Δϕ), amounts of binding of probe in the presence of Δϕ V r volume of r region ( r = o, mo, mi and i) X r amounts of probe residing at r region ( r = o, mo, mi and i) Δϕ membrane potential with reference to the extra-vesicular space Δϕ app apparent membrane potential which is calculated with neglection of bound probe μ o standard chemical potential of probe in aqueous solution μ r standard chemical potential of probe in r region ( r = mo, mi)

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UNCOUPLING Of DETECTABLE O-2 EVOLUTION fROM THE APPARENT S-STATE TRANSITIONS IN PHOTOSYSTEM-II BY LAUROYLCHOLINE CHLORIDE - POSSIBLE IMPLICATIONS IN THE PHOTOSYNTHETIC WATER-SPLITTING MECHANISM

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BIOCHIMICA ET BIOPHYSICA ACTA 1985年第1期809卷 125-136页

作者： WYDRZYNSKI, T HUGGINS, BJ JURSINIC, PA STAND OIL CO INDIANA CORP RES NAPERVILLE IL 60566 USA USDA ARS NO REG RES CTR PEORIA IL 61604 USA

Recently we have introduced the use of choline/fatty acid derived compounds, in particular lauroylcholine chloride (LCC), to probe selectively Photosystem II (PS II) structure and function (Wydrzynski, T. and Huggins, B.J. (1983) in The Oxygen-Evolving System of Photosynthesis (Inoue, Y., Crofts, A.R., Govindjee, Murata, N., Renger, G. and Satoh, K., eds.), pp. 265-272, Academic Press Tokyo, Japan). In this paper we report an unusual condition in thylakoid membrane samples at relatively low amounts of LCC in which detectable O2 evolution cannot be measured, yet electron flow through PS II is near normal without added electron donors. LCC does not appear to interfere with the O2 yield measurements directly nor act as an electron donor itself after the Tris block. Under this condition, steady state and flash O2 yield measurements show no O2 release or uptake, while steady-state ferricyanide photoreduction and the variable component of the chlorophyll .alpha. fluorescence transient remains at more than 50% of the control. The photoreduction of the primary quinone acceptor, QA, measured by microsecond range chlorophyll .alpha. fluorescence continues for a minimum of 200 single turnover excitation light flashes. Most importantly, the yield of the 35 .mu.s component of the chlorophyll .alpha. delayed fluorescence remains at approx. 65% of the control and oscillates with a normal period four over two cycles, indicating the normal cycling of the S-state transitions in PS II. Thus, it appears that PS II can operate normally without detectable O2 evolution. The question remains as to whether water is still being photooxidized under this condition without the release of the dioxygen product, or whether there is another source of electrons. The results are interpreted in terms of the possible existence of an additional water binding component (termed ''H'') in PS II and a concerted oxidation reaction mechanism for photosynthetic water splitting.

关键词： Photosystem II Water splitting Lauroylcholine chloride State transition (Pea, Spinach chloroplast) Chl chlorophyll DCIP 2,6-dichlorophenolin-dophenol DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea f max maximum fluorescence level f 0 instantaneous fluorescence level Δf variable fluorescence ( f max − f 0 ) H theoretical water-binding component Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid I 50 50% inhibition point with respect to a control measurement LCC lauroylcholine chloride M proposed managanese-containing charge-accumulating intermediate P-680 reaction center chlorophyll a molecule Phe reaction center pheophytin molecule PQ plastoquinone PS Photosystem Q A primary Q B secondary quinone acceptors S-states the S n ( n = 0, 1, 2, 3, 4) states of Photosystem II as governed by the oxidation level of M Z first electron donor to P-680 Tris 2-amino-2-hydroxymethylpropane-1,3-diol

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A NEW STATISTIC IN THE ONE-WAY MULTIVARIATE-ANALYSIS Of VARIANCE

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COMPUTATIONAL STATISTICS & DATA ANALYSIS 1985年第4期2卷 297-315页

作者： NATH, R PAVUR, R MEMPHIS STATE UNIV FOGELMAN COLL BUSINESS & ECONDEPT MANAGEMENTMEMPHISTN 38152 N TEXAS STATE UNIV COLL BUSINESSDENTONTX 76203

In the multivariate one-way analysis of variance a test statistic based solely on the rank orders of the data is proposed. In the two group case the statistic simplifies to a test of Puri and Sen [19]. Monte Carlo simulation techniques are used to evaluate the performance of the test statistic under various distributions. These evaluation include the simulated significance levels and power functions. By the nature of the proposed test it is evident that the new procedure is easier to implement than other existing rank based tests.

关键词： Wilks' lambda MANOVA Rank transformation Monte Carlo simulation Rao's f -approximation

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THE ONTOGENY Of ESTROGEN-RECEPTORS - BRAIN AND PITUITARY

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DEVELOPMENTAL BRAIN RESEARCH 1985年第2期19卷 183-194页

作者： KEEfER, D HOLDEREGGER, C INST TOXICOL SCHWERZENBACH SWITZERLAND

The prenatal and neonatal development of estrogen receptors in the CNS of the mouse was studied. [3H]Diethylstilbestro (DES) was injected into pregnant mice on days 4, 7, 10, 13, 14, 15 and 17 of gestation or into neonates. DES is an estrogen agonist that circumvents the .alpha.-fetoprotein barrier, thereby gaining access to intracellular estrogen receptors. Whole embryos, fetuses or neonates were rapidly frozen and processed for autoradiography 60 min after injection. Although the transplacental movement of the isotope was confirmed in all age groups, evidence for nuclear estrogen receptors was not seen in the brain until day EW14. On this day a few labeled cells first appeared in the basal hypothalamus, preoptic area, amygdala, midbrain and spinal cord. The number and the labeling intensity of target cells increased in each of these areas on days E15, E17 and P[postnatal]0. The first appearance of estrogen receptors closely follows the reported birthdates of neurons in these regions.

关键词： estrogen estrogen receptor diethylstilbestrol fetus brain pituitary AH anterior hypothalamus arc n. arcuatus hypothalami BH basal hypothalamus bst n. interstitialis striae terminalis CA commissura anterior CO chiasma opticum co n. amygdaloideus corticalis f fornix H hippocampus HP t. habenulopeduncularis LV ventricularis lateralis ma n. amygdaloideus medialis mb mammillary body MT t. mammillothalamicus pa n. paraventricularis PH posterior hypothalamus pi n. periventricularis PIT pituitary gland pom n. preopticus medialis pv n. premammillaris ventralis TG trigeminal ganglion TO t. opticus V ventriculus tertius vm n. ventromedialis hypothalami

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MUTATIONAL AND PSEUDOMUTATIONAL EffECTS Of 5-BROMODEOXYURIDINE IN HUMAN-LYMPHOBLASTS

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MUTATION RESEARCH 1985年第1期151卷 95-108页

作者： LIBER, HL CALL, KM MASCIOLI, DA THILLY, WG MIT DEPT APPL BIOL SCI TOXICOL GRP ROOM E18-666 CAMBRIDGE MA 02139 USA

The effects of 5-bromodeoxyuridine (BrdUrd) were studied at 2 genetic loci in diploid human lymphoblast cells. In thymidine kinase heterozygotes (tk+/-), a 2-h dose of BrdUrd induced a transient, non-heritable resistance to the thymidine analog, trifluorothymidine (f3TdR). This phenomenon was called pseudomutation and affected cells acquire the ability to survive in the presence of f3TdR and then, after degradation of f3TdR in the medium, return to an apparently normal wild-type state. BrdUrd incorporation into DNA as a thymidine analog may be responsible for the effect, which is interpreted as a temporary loss of thymidine kinase activity. This effect is not seen in tk+/+ homozygotes. At the hypoxanthine-guanine phosphoribosyl transferase locus in tk+/- heterozygotes, BrdUrd did not induce a permanent, heritable resistance to 6-thioguanine (gene locus mutation). Such mutations were detected only in the tk+/+ homozygote and only at external BrdUrd concentrations considerably higher than those which saturate the uptake of BrdUrd into DNA as a thymidine analog. The reduced TK enzyme levels (30%) in the heterozygote prevent the build-up of a sufficiently high intracellular BrdUrd triphosphate pool to promote the misincorporations as deoxycytidine triphosphate which may be responsible for gene locus mutation.

关键词： BMS butylmethane sulfonate BrdUrd 5-bromodeoxyuridine BrdUTP 5-bromodeoxyuridine triphosphate CdR deoxycytidine cfu/w colony-forming units per well CHAT cytidine-hypoxanthine-aminopterin-thymidine CHT cytidine-hypoxanthine-thymidine dCTP deoxycytidine triphosphate f 3 TdR trifluorothymidine (2′-deoxy-5-trifluoromethyluridine) hgprt hypoxanthine-guanine phosphoribosyl transferase NC non-cycling cells RPMI RPMI 1640 growth medium (with 10% horse or fetal calf serum) TdR deoxythymidine 6TG 6-thioguanine TK thymidine kinase enzyme, tk , thymidine kinase locus

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TIME-RESOLVED PICOSECOND fLUORESCENCE-SPECTRA Of THE ANTENNA CHLOROPHYLLS IN CHLORELLA-VULGARIS - RESOLUTION Of PHOTOSYSTEM-I fLUORESCENCE

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BIOCHIMICA ET BIOPHYSICA ACTA 1985年第2期807卷 155-167页

作者： HOLZWARTH, AR WENDLER, J HAEHNEL, W RUHR UNIV BOCHUM D-4630 BOCHUM 1 GERMANY

The time-resolved fluorescence emission and excitation spectra of C. vulgaris cells were measured by single-photon timing with picosecond resolution. In a 3-exponential analysis the time-resolved excitation spectra recorded at 685 and 706 nm emission wavelength with closed PS II reaction centers show large variations of the preexponential factors of the different decay components as a function of wavelength. At .*** = 685 nm the major contribution to the fluorescence decay originates from 2 components with lifetimes of 2.1-2.4 and 1.2-1.3 ns. A short-lived component with lifetimes of 0.1-0.16 ns of relatively small amplitude is found. When the emission is detected at 706 nm, the short-lived component with a lifetime of less than 0.1 ns predominates. Time-resolved emission spectra using .*** = 630 or .*** = 652 show a spectral peak of the 2 longer-live components at about 680-685 nm, whereas the fast component is red-shifted as compared to the others and shows a maximum at about 690 nm. The emission spectrum observed upon excitation at 696 nm with closed PS II reaction centers shows a large increase in the amplitude of the fast component with a lifetime of 80-100 ps as compared to that at 630 nm excitation. At almost open photosystem II (PS II) reaction centers (f0), the lifetime of the fast component decreased from 150-160 ps at 682 nm to less than 100 ps at 720 nm emission wavelength. Apparently, at least 2 pigment pools contribute to the fast component. One is attributed to PS II and the other to photosystem I (PS I). They have lifetimes of .apprx. 180 ps and 80 ps, respectively. The 80 ps (PS I) contribution has a spectral maximum slightly below 700 nm, while the 180 ps (PS II) spectrum peaks at 680-685 nm . The spectra of the middle decay component .tau.m and its sensitivity to inhibitors of PS II suggest that this component is not preferentially related to LHC II [light harvesting complex] but arises mainly from Chl a pigments probably associ

关键词： fluorescence decay Chlorophyll fluorescence Green alga Photosynthesis Photosystem I Photosystem II (Chlorella, kinetics) LHC, light-harvesting complex Chl, chlorophyll DCMU, 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea PS, Photosystem f 0 , fluorescence level at fully open PS II centers f max , fluorescence level at fully closed PS II centers f var , variable fluorescence

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PRODUCTION Of AUTO-ANTI-IDIOTYPIC ANTIBODY DURING THE NORMAL IMMUNE-RESPONSE .12. AN ENZYME-LINKED IMMUNOSORBENT-ASSAY fOR AUTO-ANTI-IDIOTYPE ANTIBODY

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JOURNAL Of IMMUNOLOGICAL METHODS 1985年第2期79卷 231-237页

作者： GIBBONS, JJ GOIDL, EA SHEPHERD, GM THORBECKE, GJ SISKIND, GW CORNELL UNIV MED CTR COLL MED DEPT MED DIV ALLERGY & IMMUNOL NEW YORK NY 10021 USA NYU SCH MED DEPT PATHOL NEW YORK NY 10016 USA

An enzyme-linked immunosorbent assay (ELISA) to detect anti-idiotype (Id) antibody was described. Using this assay auto-anti-Id was detected in the serum of aged mice immunized with 2,4,6-trinitrophenylated-ficoll (TN... 详细信息

关键词： auto-anti-idiotype idiotype ELISA for anti-idiotype BGG bovine gamma globulin BSA bovine serum albumin f ficoll Id idiotype Ig immunoglobulin PfC plaque-forming cell(s) TNP 2,4,6-trinitrophenyl group TNP-f trinitrophenyllysyl-ficoll

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