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MONOCLONAL-ANTIBODIES TO MITOCHONDRIAL COUPLING fACTOR-B

fEBS LETTERS 1985年第1期179卷 143-147页

作者： JOSHI, S KANTHAM, L KAPLAY, S SANADI, DR HARVARD UNIV SCH MEDDEPT BIOL CHEMBOSTONMA 02115 BOSTON BIOMED RES INST DEPT CELL PHYSIOLBOSTONMA 02114

Two monoclonal antibodies (MAb I and IV) have been prepared which showed high and specific reactions towards bovine heart mitochondrial coupling factor B (fB). Both have been identified as sub-type IgG1 of mouse immunoglobulins. MAb I reacts with purified and functionally active fB, alkylated or oxidized forms of fB and even with peptides formed on digestion of fB with trypsin. When used together, MAb I and IV reacted with fB in immunoblots of normal and urea treated samples of mitochondria, submitochondrial particles, ammonia-EDTA extracted particles, and H+-ATPase. Both MAbs inhibited fB-stimulated ATP-dependent reverse electron flow activity when fB was incubated with the antibody either before or after its addition to fB-deficient AE-particles. Reactivity of MAb I towards fB declined upon exposure of fB to guanidine HC1 while reactivity of MAb IV remained unaltered.

关键词： Coupling factor B H + -ATPase Mitochondria Monoclonal antibody f B , coupling factor B AE-particles, ammonia-EDTA particles ETP H , electron transport particles ELISA, enzyme-linked immunosorbent assay PBS-Tween, 10 mM phosphate buffer, 0.9% NaCl and 0.05% Tween-20 MAb, monoclonal antibody

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MODIfICATION Of PHOTOSYSTEM-II ACTIVITY BY PROTEIN-PHOSPHORYLATION

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fEBS LETTERS 1985年第1期181卷 83-87页

作者： HODGES, M PACKHAM, NK BARBER, J AFRC Photosynthesis Research Group Imperial College London SW7 2BB England

Phosphorylation of proteins within pea thylakoid membranes decreases photosystem II (PSII) mediated electron transfer at saturating light intensities which, according to changes in room temperature chlorophyll fluorescence transients, is due to a modification in the electron transfer from Q A to Q B . However, a previously reported increase in the ability of DCMU to inhibit PS2 electron flow to DCPIP, as a consequence of protein phosphorylation, was not observed, although changes in DCMU efficacy were found to depend upon the redox state of the plastoquinone (PQ) pool.

关键词： Protein phosphorylation Photosystem II Chlorophyll fluorescence Herbicide inhibition PQ redox state Electron transport PS, photosystem LHC, light harvesting chl a/b protein complex DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea DCPIP, 2,6-dichlorophenol-indophenol DPC, diphenylcarbazide Tricine, N -tris(hydroxymethyl)methylglycine f o , initial chlorophyll fluorescence f m , maximum chlorophyll fluorescence f i , chlorophyll fluorescence at i Q a , primary stable electron acceptor of PS II Q b , secondary stable electron acceptor of PS II

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Anomalous behavior of goldfish IgM heavy chain in sodium dodecylsulfate polyacrylamide gel electrophoresis

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Comparative Biochemistry and Physiology -- Part B: Biochemistry and 1985年第1期82卷 41-49页

作者： Wllson, Melanie R. Wang, An-Chuan fish, Wayne W. Warr, Gregory W. United States United States

1. 1. By sodium dodecylsulfate polyacrylamide gel electrophoresis, the heavy chain of the serum immunoglobulin (IgM) of the goldfish (Carassius auratus) differs not only from other studied vertebrate serum IgM heavy chains, but also from other vertebrate lymphocyte membrane IgM heavy chains including those from the goldfish itself. 2. 2. This difference, an increase in apparent M_r of approximately 5000, was investigated by assessing in comparison with the IgM heavy chains of human and rainbow trout (Salmo gairdneri) the following properties: (1) molecular size by gel filtration in denaturing buffers;(2) carbohydrate content, by direct analysis;(3) intrinsic net charge, by isoelectric focusing;(4) net hydrophobicity, deduced from amino acid analysis;and (5) sodium dodecylsulfate binding by direct measurement. 3. 3. Results indicate that goldfish IgM heavy chain is indistinguishable from other IgM heavy chains in terms of (a) its gel-filtration behavior in denaturing conditions, (b) its carbohydrate content (which is similar to trout IgM heavy chain) and (c) its intrinsic net charge and hydrophobicity. 4. 4. However, goldfish IgM does differ from the other proteins studied in its detergent-binding ability and it is this behavior that is concluded to be the cause of its unusual mobility in sodium dodecylsulfate polyacrylamide gel electrophoresis. © 1985.

关键词： C-terminus carboxyl terminus GluNH 2 glucosamine IgG immunoglobulin G IgM immunoglobulin M K av V e − V o / V t − V o : M r relative molecular mass pI isoelectric point R f relative mobility S.A. sialic acid Tris tris(hydroxymethyl)amino methane V e peak elution volume V o column void volume V t total included column volume

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ACID-HYDROLASES DURING ARTEMIA DEVELOPMENT - A ROLE IN YOLK DEGRADATION

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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 1985年第4期81卷 993-1000页

作者： PERONA, R VALLEJO, CG UNIV AUTONOMA BARCELONA FAC MEDCSICINST IVEST BIOMED ARZOBISPO MORCILLO 4 E-28029 MADRID SPAIN

A broad group of acid hydrolytic activities have been determined during Artemia development including cathepsin B acid ribonuclease, acid deoxyribonuclease, acid phosphatase, acid phosphodiesterase, .beta.-glucosidase, .beta.-N-acetylgalactosaminidase and acid lipase. These enzymes present maximum activity in nauplii, when yolk degradation is maximum. Artemia cathepsin B proteinase is able to degrade lipovitellin in vitro with a pattern similar to that found in vivo. The involvement of acid hydrolases in the degradation of yolk is suggested. In cryptobiotic embryos acid hydrolases were found associated with structures of buoyant density 1.18. The structures appeared at the electron microscope as 0.4 .mu.m diameter vesicles when revealed by acid phosphatase cytochemistry.

关键词： PhMeSO 2 f phenylmethylsulfonylfluoride ClHgPhSO 2 H p -chloromercuriphenyl sulfonic acid OH-HgBzOH p -hydroxymercuribenzoate E-64 mixture of the optical isomers N-[N- ( dl -3-transcarboxyirancarbonyl)- l -leucyl ] agmatine

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MICROTUBULE-ASSOCIATED ADENYLATE-CYCLASE

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BIOCHIMICA ET BIOPHYSICA ACTA 1985年第1期844卷 34-41页

作者： WOLff, J COOK, GH N.I.A.D.D.K. National Institutes of Health Bethesda MD 20205 United States

Twice-cycled bovine brain or rat brain microtubule protein contains an adenylate cyclase activity that passes 0.2 .mu.m filters, is activated 2-7-fold by 30 .mu.M forskolin, shows modest stimulation by fluoride (especially in the presence of added Al3+), but is virtually insensitive to added guanine nucleotides. The activity is insensitive to various hormones or Ca2+/calmodulin. The adenylate cyclase is active with both Mg2+ and Mn2+ but activity is less in the presence of Mg2+ than with Mn2+. The cyclase is inhibited by agonists of the adenosine P site. The catalytic unit of adenylate cyclase and probably small quantities of the guanine nucleotide regulatory protein, Ns, evidently are cycled along with microtubules.

关键词： Microtubule Adenylate cyclase forskolin Tubulin Calmodulin f Me 4-morpholinecthanesulfonic acid MAP microtubule-associated protein

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非晶态合金稳定性的电子判据2kf=qp条件的讨论

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贵金属 1985年第2期 30-35页

作者：冯本政杨汝森徐国枢昆明贵金属研究所昆明贵金属研究所云南大学中心实验室

用X-射线衍射分析测量Zr100-XMX（M=fe、Co、Ni、Cu和Pd）,Cu100-XTiX,Pd100-XSiX和Pd80-XSi20CrX等非晶态合金的qP值,由自由电子模型计算各合金的2kf值,并讨论2kf=qP与合金成分和负电阻率温度系数α之关系。结果表明,2kf=qP的条件是不... 详细信息

用X-射线衍射分析测量Zr100-XMX（M=fe、Co、Ni、Cu和Pd）,Cu100-XTiX,Pd100-XSiX和Pd80-XSi20CrX等非晶态合金的qP值,由自由电子模型计算各合金的2kf值,并讨论2kf=qP与合金成分和负电阻率温度系数α之关系。结果表明,2kf=qP的条件是不成立的。

关键词：非晶态合金电阻率金属玻璃精密合金电阻系数 f 判据

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PERITZ f-TEST - BASIC PROGRAM Of A ROBUST MULTIPLE COMPARISON TEST fOR STATISTICAL-ANALYSIS Of ALL DIffERENCES AMONG GROUP MEANS

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COMPUTERS IN BIOLOGY AND MEDICINE 1984年第4期14卷 437-445页

作者： HARPER, Jf UNIV TEXAS SCH MED DEPT INTERNAL MED HOUSTON TX 77025 USA UNIV TEXAS SCH MED DEPT PHARMACOL HOUSTON TX 77025 USA

Peritz'' f test was previously found to be the most robust statistical multiple comparison test able to hold all comparisons among group means to a given experimentwise error rate. A BASIC program which will perform this test quickly on a desk-top microcomputer, needing fewer than 11 kbytes [kilobyte] of RAM memory, was presented. The program was run in the author''s laboratory using an inexpensive VIC-20 computer.

关键词： BASIC Experimentwise error rate Microcomputer Multiple comparison test Peritz' f test Statistics

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fORMATION Of 2-AMINO-3,8-DIMETHYLIMIDAZO[4,5-f]QUINOXALINE IN A MODEL SYSTEM BY HEATING CREATININE, GLYCINE AND GLUCOSE

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MUTATION RESEARCH 1984年第3期126卷 239-244页

作者： JAGERSTAD, M OLSSON, K GRIVAS, S NEGISHI, C WAKABAYASHI, K TSUDA, M SATO, S SUGIMURA, T UNIV LUND CTR CHEM DEPT NUTR S-22007 LUND 7 SWEDEN SWEDISH UNIV AGR SCI DEPT CHEM & MOLEC BIOL S-75007 UPPSALA SWEDEN NATL CANC CTR RES CTR DIV BIOCHEM CHUO KU TOKYO 104 JAPAN

A mixture of creatinine, glucose and Gly was heated in diethylene glycol containing 14% water for 2 h at ***. C, and the mutagens formed were purified by XAD-2 column chromatography, acid-base partition. Sephadex LH-20 column chromatography, blue cotton treatment and HPLC [high performance liquid chromatography]. Two mutagenic substances were isolated by HPLC. The major mutagen was identified by its UV absorption and mass and NMR spectra as 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, which was originally isolated from fried beef. The idea that creatinine, amino acids and sugars present in meat are precursors in the formation of the mutagenic imidazoquinoxaline derivative is supported.

关键词： Trp-P-1 3-amino1,4-dimethyl-5 H -pyrido[4,3- b ]indole 62450-06-0 Trp-P2 3-amino-1-methyl-5 H -pyrido[4,3- b ]indole, 62450-07-1 Glu-P-1 2-amino-6-methyldipyrido[1,2- a :3′,2′- d ]imidazole, 67730-11-4 Glu-P-2 2-aminodipyrido[1,3- a :3′,2′- d ]imidazole, 67730-10-3 Orn-P-1 4-amino-6-methyl-1 H -2,5,10,10b-tetraazafluoranthene, 78859-36-6 Phe-P-1 2-amino-5-phenylpyridine, 33421-40-8 AαC 2-amino-9 H -pyrido[2,3- b ]indole, 26148-68-5 MeAαC 2-amino-3-methyl-9 H -pyrido[2,3- b ]indole, 68006-83-7 IQ 2-amino-3-methylimidaza[4,5- f ]quinoline, 76180-96-6 MeIQ 2-amino-3,4-dimethylimidazo[4,5- f ]quinoline, 77094-11-2 MeIQx 2-amino-3,8-dimethylimidazo[4,5- f ]quinoxaline, 77500-04-0 HPLC high performance liquid chromatography

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ELECTRON-MICROSCOPY Of BEEf-HEART MITOCHONDRIAL f1-ATPASE

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fEBS LETTERS 1984年第2期167卷 285-290页

作者： TSUPRUN, VL MESYANZHINOVA, IV KOZLOV, IA ORLOVA, EV MV LOMONOSOV STATE UNIV BELOZERSKII MOLEC & BIOORGAN CHEM LAB MOSCOW 117234 USSR

The quaternary structure of isolated and membrane-bound f1-ATPase (submitochondrial particles) has been studied by electron microscopy. A model of the molecule has been proposed: six protein masses are arranged in two layers approximately at the vertices of a triangular antiprism. Computer averaging of the images showed that the frontal view of the molecule can be approximately characterized by mirror plane symmetry.

关键词： f 1 -ATPase Enzyme structure Electron microscopy

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INACTIVATION Of BEEf-HEART MITOCHONDRIAL f1-ATPASE BY THE 2',3'-DIALDEHYDE DERIVATIVES Of ADENINE-NUCLEOTIDES

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fEBS LETTERS 1984年第1期169卷 101-106页

作者： DEMELO, Df SATRE, M VIGNAIS, PV CEN DEPT RECH FONDAMENTALEBIOCHIM LABCNRSERA 903 INSERMU191 F-38041 GRENOBLE FRANCE

Beef heart mitochondrial f1-ATPase was inactivated by the 2',3'-dialdehyde derivatives of ATP, ADP and AMP (oATP, oADP, oAMP). In the absence of Mg2+, inactivation resulted from the binding of 1 mol nucleotide analog per active unit of f1. The most efficient analog was oADP, followed by oAMP and oATP. Complete inactivation was correlated with the binding of about 11 mol [14C]oADP/mol f1. After correction for non-specific labeling, the number of specifically bound [14C]oADP was 2-3 mol per mol f1. By SDS-polyacrylamide gel electrophoresis, [14C]oADP was found to bind covalently mainly to the alpha and beta subunits. In the presence of Mg2+, oATP behaved as a substrate and was slowly hydrolyzed.

关键词： f 1 -ATPase Dialnucleotide Affinity labeling Enzyme inactivation f 1 , beef heart soluble mitochondrial ATPase Tra, triethanolamine oAdN, 2′,3′-dialdehyde derivatives of ATP, ADP and AMP (oATP, oADP, oAMP)

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