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fusarium species and enniatin mycotoxins in wheat, durum wheat, triticale and barley harvested in france

MYCOTOXIN RESEARCH 2019年第4期35卷 369-380页

作者： Orlando, Beatrice Grignon, Guenole Vitry, Cindy Kashefifard, Kambiz Valade, Romain ARVALIS Inst Vegetal Stn Expt F-91720 Boigneville France ARVALIS Inst Vegetal Ave Lucien Bretignieres F-78850 Thiverval Grignon France

Contamination with enniatins A, A1, B and B1 was investigated in 1240 samples of small grain cereals (470 wheat, 260 durum wheat, 282 spring barley, 172 triticale and 56 winter barley) from the french harvests of 2012 to 2014. Associations with fusarium avenaceum, f. tricinctum and f. poae were assessed, with the identification of fusarium species by real-time PCR and mycotoxin quantification by LC-MS/MS. Enniatins were common in the fields sampled. Enniatin concentrations varied between years but were consistently highest on spring barley (mean values of 199 to 1316 mu g/kg) and triticale (mean values from 131 to 1218 mu g/kg), and lower on wheat (mean values from 47 to 142 mu g/kg) and durum wheat (mean values from 55 to 596 mu g/kg). The concentrations of the various enniatins were strongly correlated with each other (Pearson's correlation coefficient of 0.61 to 0.98). Enniatin B was the most frequent (68% of the total enniatin content), followed by enniatin B1 (22%), enniatin A1 (7%) and enniatin A (3%). fusarium species were quantified by calculating arithmetic mean total DNA levels. f. tricinctum was the most abundant (0.177 pg/ng total DNA), followed by f. avenaceum (0.141 pg/ng total DNA) and f. poae (0.091 pg/ng total DNA). Total DNA levels for each species, and the predominant species varied between years and crops. Small grain cereal species (p value < 0.001), harvest year (p value = < 0.001) and the presence of f. avenaceum (p value < 0.001), f. tricinctum (p value < 0.001) or f. poae (p value = 0.017) affected enniatin content. f. tricinctum was the leading enniatin producer on durum wheat (29% to 45%) and spring barley (23 to 37%). f. avenaceum produced large amounts of enniatins on durum wheat (13% to 17%) and wheat (1% to 18%) and was the leading producer on triticale (30% to 55%). f. poae made a minor contribution on all crops, never accounting for more than 2% of total enniatin content. Enniatins are, thus, highly prevalent in french small grain ce

关键词： Enniatins f tricinctum f avenaceum f poae

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Estimation of the number of feeding arteries of spinal arteriovenous malformations by using three-dimensional digital subtraction angiography

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EUROPEAN SPINE JOURNAL 2019年第4期28卷 842-848页

作者： Takamiya, Soichiro Osanai, Toshiya Seki, Toshitaka fujima, Noriyuki Hida, Kazutoshi Asano, Takeshi Yamazaki, Kazuyoshi Hamauchi, Shuji Sasamori, Toru Ono, Kota Terasaka, Shunsuke Houkin, Kiyohiro Hokkaido Univ Fac Med Dept Neurosurg Sapporo Hokkaido Japan Hokkaido Univ Grad Sch Med Sapporo Hokkaido Japan Hokkaido Univ Hosp Dept Diagnost & Intervent Radiol Sapporo Hokkaido Japan Sapporo Azabu Neurosurg Hosp Dept Neurosurg Sapporo Hokkaido Japan Chiba Med Ctr Dept Neuroendovasc Chiba Japan Hokkaido Univ Hosp Clin Res & Med Innovat Ctr Sapporo Hokkaido Japan

PurposeSpinal angiography is the gold standard for evaluation or diagnosis of spinal arteriovenous malformations (AVMs). However, some feeding arteries might be overlooked when multiple feeders exist. This study aimed to retrospectively review cases of spinal intra-dural AVMs, which were identified by three-dimensional digital subtraction angiography (3D-DSA), and attempted to estimate the number of feeding *** retrospectively reviewed patients with spinal intra-dural AVMs who underwent 3D-DSA at Hokkaido University Hospital from January 2005 to December 2016. We selected 9 patients in whom we could obtain data of multi-planar reconstruction of 3D-DSA. We measured the computed tomography (CT) values of feeding arteries and draining veins. The CT values represented the averages of maximum CT values of 5 continuous axial slices. The ratio of the CT value of feeders to that of drainers (f/D ratio) was calculated. The correlation between the f/D ratio and the number of feeders was examined with Pearson's correlation *** average number of feeders was 2.3 (1-4), and the number of feeders was significantly positively correlated with the f/D ratio (r=0.855, P=.003).ConclusionsWe conclude that the number of feeding arteries of spinal intra-dural AVMs can be estimated by using the f/D ratio obtained from 3D-DSA [GRAPHICS]

关键词： Spinal arteriovenous malformations 3D-DSA f D ratio Estimation Perimedullary AVf Intramedullary AVM

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NEDD4 Is Involved in Inflammation Development during Keloid formation

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JOURNAL Of INVESTIGATIVE DERMATOLOGY 2019年第2期139卷 333-341页

作者： fujita, Munezumi Yamamoto, Yuhei Jiang, Jing-Jing Atsumi, Toru Tanaka, Yuki Ohki, Takuto Murao, Naoki funayama, Emi Hayashi, Toshihiko Osawa, Masayuki Maeda, Taku Kamimura, Daisuke Murakami, Masaaki Hokkaido Univ Inst Genet Med Div Mol Psychoimmunol Sapporo Hokkaido 0600815 Japan Hokkaido Univ Grad Sch Med Sapporo Hokkaido Japan Hokkaido Univ Fac Med Dept Plast & Reconstruct Surg Sapporo Hokkaido Japan

Keloids mark a chronic inflammatory disease characterized by a fibroproliferative disorder of the skin. A genome-wide association study showed that single-nucleotide polymorphism rs8032158 in the neural precursor cell-expressed NEDD4 gene, which has six protein-coding transcript variants (TVs), is genetically linked to keloids. Here, we show that the high frequency of risk allele C in rs8032158 in keloid patients is associated with a selectively higher expression of TV3 of NEDD4 to activate the Nf-kB pathway. Comparisons of keloid scars with normal skin samples that do not have the single-nucleotide polymorphism allele and were derived from different anatomical sites showed stronger expressions of NEDD4 TV3 and activated forms of Nf-kB and STAT3 in keloid scars. forced expression or selective knockdown of NEDD4 TV3 increased or decreased Nf-kB activation in vitro. furthermore, NEDD4 knockdown suppressed Nf-kBedependent inflammation development in vivo. Mechanistic analysis showed that NEDD4 TV3 is involved in Nf-kB activation through its association with the adaptor protein RIP. These results suggest that NEDD4 TV3 is a potential diagnostic marker and therapeutic target for chronic skin diseases, including keloid.

关键词： f phenylalanine GWAS genome-wide association study phospho- phosphorylated siRNA small interfering RNA SNP single-nucleotide polymorphism TNf-α tumor necrosis factor-α TV transcript variant

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Kinetics and Thermodynamics of Adsorption of fluorides by ZeoSonNafe

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MRS ADVANCES 2019年第59-60期4卷 3215-3221页

作者： Lopez-Castillo, Jessica Macedo-Miranda, G. Martinez-Gallegos, S. Ordonez, E. Illescas, J. Barragan-Pena, P. Gomez-Beltran, Guillermina Tecnol Nacl Mexico Inst Tecnol Toluca Av Tecnol S-N Bellavista 52140 Metepec Mexico Inst Nacl Invest Nucl Carretera Mexico Toluca S-N La Marquesa 52750 Ocoyoacac Mexico Tecnol Nacl Mexico Inst Tecnol Nogales Ave Tecnol 911 Col Granja Mexico City 84065 DF Mexico Univ Tecnol Valle Toluca Del Dept Dist Fed Km 7-5 Santa Maria Atarasquillo 52044 Mexico

The present study investigated the adsorption of fluorides in natural zeolites clinoptilolite-type, sourced in Sonora, Mexico, and modified with fe. Characterization of the material was carried out along with determination of kinetic parameters varying time and concentration of reagents to calculate maximum adsorption capacity. To determine thermodynamic parameters temperature was varied for accurate characterization of the adsorption process. It was found that the zeolite fixed fe in its structure without model significant modification, likewise when put in contact with a fluoride solution (f-), it was capable of taking up these ions through adsorption due to the interaction between the sorbato and the pollutant. The kinetics best fit Pseudo-second-order model with Qmax=0.91 mgg(-1), therefore it indicates that the process is reversible, spontaneous and endothermic what can favor the reuse of the material.

关键词： adsorption f zeolite

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Creation and anthropology: NfS Grundtvig as humanist and feminist

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DIALOG-A JOURNAL Of THEOLOGY 2021年第2期60卷 137-144页

作者： Pedersen, Else Marie Wiberg Aarhus Univ Theol Jens Chr Skousvej 3 DK-8000 Aarhus Denmark

This article focuses on Scandinavian creation theology by way of the anthropology of the Happy Dane, the theologian, author, hymn writer, poet, and pastor Nicolai frederik Severin Grundtvig (1783-1872). After having given two examples of Grundtvig's and Grundtvigians' encounter with USA in the past, it is demonstrated how the Lutheran Grundtvig, based on his positive and organic creation theology, fought ideas of creational hierarchies, whether slavery or subjection of women. Grundtvig logically and naturally connected his creation theology to a humanist anthropology of which not the devil but God was Creator and sole subject. As in Galatians 3:28, this encompassed a feminist view as he acknowledged two equal sexes and genders: all humans are equal regardless of color of the skin, sex, or belief and hence should have human rights equally.

关键词： creation theology of equality every human as male and female feminist humanist N f S Grundtvig

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斯里兰卡CKDu病区地下水源饮用水关键问题及解决策略

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环境工程学报 2020年第8期14卷 2100-2111页

作者：郑利兵 COORAY Titus 钟慧 WERAGODA Sujithra WEERASOORIYAE Rohan MAKEHELWALA Madhubhashini 魏源送中国科学院生态环境研究中心中国科学院-发展中国家科学院水与环境卓越中心北京100085 中国科学院生态环境研究中心环境模拟与污染控制国家重点联合实验室北京100085 中国科学院生态环境研究中心水污染控制实验室北京100085 斯里兰卡国家供排水部斯里兰卡卡图迦士托塔20800 斯里兰卡国家基础研究院斯里兰卡康提20000

不明原因肾病(CKDu)是斯里兰卡亟需解决的重要民生问题,其地下水源饮用水中的溶解性污染物可能是重要的致病原因。系统分析了北部中心省内高、低、非发病区及对照区(HR、LR、NR、CR)干湿季地下水的水化学特征和DOM特性,结果表明:地下水... 详细信息

不明原因肾病(CKDu)是斯里兰卡亟需解决的重要民生问题,其地下水源饮用水中的溶解性污染物可能是重要的致病原因。系统分析了北部中心省内高、低、非发病区及对照区(HR、LR、NR、CR)干湿季地下水的水化学特征和DOM特性,结果表明:地下水主要呈Ca-Mg-HCO3型,硬度和f-超标率较高,是地下水作为饮水安全保障最关键的指标;地下水中DOM主要为自生源,芳香性和腐熟化程度较低,主要为富里酸、腐殖酸和小分子蛋白质类物质;湿季DOM浓度较高,是fe浓度增加的关键原因;而干季地下水中微生物活性较高,腐殖质和蛋白质发生分解,小分子络氨酸/赖氨酸的浓度增加。同时,发现不同区域地下水中DOM的生物利用性表现为HRf-软化-Nf-消毒"的处理策略,通过有机物吸附处理、阴离子交换除f、软化除硬分离预处理及Nf深度处理等技术有效解决地下水中DOM、f-、Ca^2+和Mg^2+超标的问题,实现饮用水的供给。本研究全面分析了CKDu病区地下水中的污染物组成,并提出解决策略,可为斯里兰卡饮用水安全保障提供支撑。

关键词：不明原因肾病溶解性有机物硬度 f 地下水纳滤

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Maternal mitochondrial inheritance in two fusarium pathogens of prickly ash (Zanthoxylum bungeanum) in northern China

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MYCOLOGIA 2019年第2期111卷 235-243页

作者： Zhou, Xue Cao, Zhi-Min Liu, Xin Kim, Hye-Seon Proctor, Robert H. O'Donnell, Kerry Northwest A&F Univ Coll Forestry Taicheng Rd Yangling 712100 Shaanxi Peoples R China ARS Mycotoxin Prevent & Appl Microbiol MPM Res Unit Natl Ctr Agr Utilizat Res USDA Peoria IL 60604 USA

Mitochondrial inheritance in fusarium zanthoxyli and f. continuum, two canker-inducing pathogens of prickly ash (Zanthoxylum bungeanum) in northern China, was investigated by genotyping ascospore progeny obtained from laboratory crosses. Polymorphic regions of the mitochondrial genomes (mitogenomes) that contained indels and single-nucleotide polymorphisms (SNPs) were identified via comparative analyses of the complete mitogenomes of the parents used in the intraspecific crosses. A reciprocal genetic cross of f. zanthoxyli NRRL 66714 x NRRL 66285, and a separate cross of f. continuum f NRRL 66286 x o NRRL 66218, revealed that mitochondria were only inherited from the maternal parent. In addition, the reciprocal cross demonstrated that mitochondrial inheritance is not linked to mating type. Gene order in the circular mitogenomes of the prickly ash pathogens was identical to that previously reported for other fusaria and members of the Hypocreales, except that the TRNL tRNAs were duplicated in f. zanthoxyli NRRL 66714. The genomes contained 14 polypeptide-encoding genes involved in oxidative respiration, one intron-encoded ribosomal protein (rps3) gene, two ribosomal RNA (rRNA) genes, and 26-28 tRNA genes. The f. zanthoxyli mitogenomes were 80.9 and 98.7 kb in length, whereas those of f. continuum were considerably shorter and nearly identical in length at 63.4 kb. The significant differences in mitogenome length were primarily due to variable numbers of introns and open reading frames (ORfs) encoding hypothetical proteins.

关键词： Comparative mitochondrial genomics f continuum f zanthoxyli genetic crosses mitogenome uniparental inheritance whole genome

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TOPOLOGÍA Y fUNCIÓN DE LAS SUBUNIDADES INTRÍNSECAS DE LA MEMBRANA DE LAS f 1 f O -ATP SINTASA MITOCONDRIALES

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TIP 2017年第2期20卷 29-47页

作者： Lorenzo Sánchez-Vásquez Diego González-Halphen Instituto de Fisiología Celular Universidad Nacional Autónoma de México (UNAM). Apartado Postal 70-600 Deleg.Coyoacán C.P. 04510 Ciudad de México México

La f 1 f O -ATP sintasa es un complejo enzimático que se encuentra ampliamente distribuido en las membranas transductoras de energía. Todas las ATPasas, incluidas las mitocondriales, cloroplastídicas y bacterianas, comparten similitudes estructurales y funcionales. Sin embargo, hay diferencias en su composición que dependen de la especie, siendo más compleja en organismos como Saccharomyces cerevisiae o Bos taurus . Es por ello que una mejor comprensión de la estructura de la f 1 f O -ATP sintasa contribuirá a un mayor conocimiento a nivel molecular tanto de la función como de la regulación de este complejo enzimático. En la actualidad, se sabe muy poco acerca de la organización estructural de las subunidades de la región f O. Considerando lo anterior, en este trabajo se presenta información concerniente a las proteínas intrínsecas del dominio f O de las ATP sintasas más investigadas a la fecha, así como de algunas otras subunidades de membrana que se encuentran presentes en organismos menos estudiados. The f 1 f O -ATP synthase is a complex widely distributed in energy-transducing membranes. All ATPases, including the mitochondrial, chloroplastic and bacterial, share structural and functional similarities. However, there are differences in their composition that depend on the species, being more complex in organisms such as Saccharomyces cerevisiae or Bos taurus . This is why, a better understanding of the f 1 f O -ATP synthase structure will contribute to a greater knowledge at a molecular level, both of the function, and the regulation of this enzymatic complex. At present, very little is known about the structural organization of the subunits from the f O domain. Considering the former, this paper presents information concerning the intrinsic membrane proteins from the most researched f 1 f O -ATP synthases to date, as well as some other membrane subunits present in less studied organisms.

关键词： algas clorofíceas estructura f 1 f O -ATP sintasa mitocondrial proteínas membranales subunidades atípicas chlorophycean algae structure mitochondrial f 1 f O -ATP synthase membrane proteins atypical subunits

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Application of the improved f -expansion method with Riccati equation to find the exact solution of the nonlinear evolution equations

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Journal of the Egyptian Mathematical Society 2017年第1期25卷 13-18页

作者： Md. Shafiqul Islam Kamruzzaman Khan M. Ali Akbar Department of Mathematics Pabna University of Science and Technology Pabna 6600 Bangladesh Department of Applied Mathematics University of Rajshahi Rajshahi 6205 Bangladesh

In this article, we pay attention to the analytical method named, the improved f -expansion method combined with Riccati equation for finding the exact traveling wave solutions of the Benney–Luke equation and the Phi-4 equation. By means of this method we have explored three classes of explicit solutions-hyperbolic, trigonometric and rational solutions with some free parameters. When the parameters are taken as special values, the solitary wave solutions are originated from the traveling wave solutions. Our outcomes disclose that this method is very active and forthright way of formulating the exact solutions of nonlinear evolution equations arising in mathematical physics and engineering.

关键词： Improved f -expansion method Benney–Luke equation Phi-4 equation Exact solution NLEEs 02.30.Jr 02.70.Wz 05.45.Yv 94.05.fg 35K99 35P05 35P99

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灰毡毛忍冬花蕾中苷类化学成分研究

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中草药 2020年第2期51卷 287-292页

作者：梅玉丹李海波王振中于洋姚新生肖伟江苏康缘药业股份有限公司江苏连云港222001 中药制药过程新技术国家重点实验室江苏连云港222001 中药提取精制新技术重点研究室江苏连云港222047 暨南大学中药及天然药物研究所广东广州510632 沈阳药科大学中药学院辽宁沈阳110016

目的研究灰毡毛忍冬Lonicera macranthoides花蕾中的苷类化学成分。方法采用HP-20、硅胶、ODS、Sephadex LH-20和制备液相等多种色谱学方法进行分离纯化,并通过理化常数和各种波谱技术鉴定化合物的结构。结果从灰毡毛忍冬干燥花蕾70%乙... 详细信息

目的研究灰毡毛忍冬Lonicera macranthoides花蕾中的苷类化学成分。方法采用HP-20、硅胶、ODS、Sephadex LH-20和制备液相等多种色谱学方法进行分离纯化,并通过理化常数和各种波谱技术鉴定化合物的结构。结果从灰毡毛忍冬干燥花蕾70%乙醇提取物中分离得到了8个化合物,分别鉴定为7,3′,4′-三甲氧基槲皮素-3-O-α-L-吡喃阿拉伯糖基-(1→6)-O-β-D-吡喃葡萄糖苷(1)、7,3′,4′-三甲氧基槲皮素-3-O-芸香糖苷(2)、槲皮素-3-O-β-D-吡喃葡萄糖苷(3)、坎卡酯苷(4)、kankanoside E(5)、betulalbuside A(6)、shomaside f(7)、amarantholidoside V(8)。结论化合物1为新化合物,命名为甲氧基槲皮素苷;化合物5~8为首次从忍冬属植物中分离得到。

关键词：山银花灰毡毛忍冬槲皮素-3-O-β-D-吡喃葡萄糖苷甲氧基槲皮素苷 kankanoside E betulalbuside A shomaside f amarantholidoside V

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