检索结果-内蒙古大学图书馆

Etched Glass microarrays with Differential Resonance for Enhanced Contrast and Sensitivity of Surface Plasmon Resonance Imaging Analysis

引用

ANALYTICAL CHEMISTRY 2011年第15期83卷 5936-5943页

作者： Linman, Matthew J. Abbas, Abdennour Roberts, Christopher C. Cheng, Quan Univ Calif Riverside Dept Chem Riverside CA 92521 USA

We report the fabrication and characterization of gold-coated etched glass array substrates for surface plasmon resonance imaging (SPRi) analysis with significantly enhanced performance, in particular image contrast and sensitivity. The etching of the glass substrate induces a variation in the resonance condition and thus in the resonance angle between the etched wells and the surrounding area, leading to the isolation of the array spot resonance with a significant reduction of the background signal. FDTD simulations show arrays with large spots and minimal spot-to-spot spacing yield ideal differential resonance conditions, which are verified by experimental results. Simulations also indicate the etched well structure exhibits enhanced SPR electric field intensity by 3-fold as compared to standard planar gold chips. Changes in the bulk sensitivity of the etched arrays have been obtained at the 10(-4) RIU level based on image intensity difference. The strong image contrast allows for improved microarray imaging analysis with easily distinguished signals from background resonance. The etched array chips are demonstrated for SPRi detection of bacterial toxins through the coating of an ultrathin SiO2 film for direct vesicle fusion that establishes a supported membrane-based biosensing interface. Protein detection with cholera toxin (CT) at 5 nM is obtained, making this chip one of the most sensitive SPR imaging substrates ever reported without a postbinding amplification scheme. Furthermore, the surface can be regenerated by Triton X-100 for repeated cycles of membrane formation, protein binding, and biomolecular removal. The reusability and enhanced performance of the etched glass array chips should find a broad range of applications, opening up new avenues for high-throughput SPR imaging detection with convenience and marked surface sensitivity.

关键词： SURFACE plasmon resonance DNA microarrays PROTEIN microarrays microarray technology CLINICAL chemistry

来源：评论

学校读者我要写书评

暂无评论

Advances in bacterial transcriptome and transposon insertion-site profiling using second-generation sequencing

引用

TRENDS IN BIOtechnology 2011年第11期29卷 586-594页

作者： Febrer, Melanie McLay, Kirsten Caccamo, Mario Twomey, Kate B. Ryan, Robert P. Univ Coll Cork BioSci Inst Dept Microbiol BIOMERIT Res Ctr Cork Ireland Genome Anal Ctr Norwich NR4 7UH Norfolk England

The arrival of second-generation sequencing has revolutionized the study of bacteria within a short period. The sequence information generated from these platforms has helped in our understanding of bacterial development, adaptation and diversity and how bacteria cause disease. Furthermore, these technologies have quickly been adapted for high-throughput studies that were previously performed using DNA cloning or microarray-based applications. This has facilitated a more comprehensive study of bacterial transcriptomes through RNA sequencing (RNA-Seq) and the systematic determination of gene function by 'transposon monitoring'. In this review, we provide an outline of these powerful tools and the in silico analyses used in their application, and also highlight the biological questions being addressed in these approaches.

关键词： TRANSPOSONS BACTERIAL genetic engineering NUCLEOTIDE sequence microarray technology MOLECULAR cloning BACTERIAL diversity ASEXUAL reproduction ADAPTATION (Biology)

来源：评论

学校读者我要写书评

暂无评论

引用

JOURNAL OF BIOLOGICAL CHEMISTRY 2011年第46期286卷 39715-39725页

作者： Gleyzer, Natalie Scarpulla, Richard C. NW Med Sch Dept Cell & Mol Biol Chicago IL USA

PGC-1-related coactivator (PRC) is a growth-regulated transcriptional cofactor that activates many nuclear genes specifying mitochondrial respiratory function. Stable PRC silencing in U2OS cells results in a complex phenotype typical of mitochondrial dysfunction including abundant abnormal mitochondria, reduced respiratory subunit expression, diminished respiratory enzymes and ATP levels, and elevated lactate production. The PRC response to metabolic stress was investigated by subjecting cells to metabolic insults including treatment with the uncoupler carbonyl cyanide 3-chlorophenylhydrazone (CCCP), expression of a dominant negative allele of nuclear respiratory factor 1 (NRF-1), and glucose deprivation. These treatments led to constitutively elevated PRC protein levels, a departure from its normal transient expression upon the initiation of cell growth. A microarray screen identified 45 genes that require PRC for their induction by CCCP. A subset of these genes specific to inflammation and cell stress was also induced by dominant negative NRF-1 and by glucose deprivation, suggesting that diverse metabolic insults converge on the same PRC-dependent inflammatory program. The PRC-dependent inflammatory response was inhibited by N-acetylcysteine, suggesting that PRC may contribute to the inflammatory microenvironment linked to oxidant signaling. The induction of this PRC-dependent program may be an early event in adaptations linked to cancer and degenerative diseases.

关键词： PROTEIN S OXIDATION-reduction reaction GENE expression MITOCHONDRIA -- Formation GROWTH factors microarray technology

来源：评论

学校读者我要写书评

暂无评论

Novel Computational Identification of Highly Selective Biomarkers of Pollutant Exposure

引用

ENVIRONMENTAL SCIENCE & technology 2011年第12期45卷 5132-5138页

作者： Weisman, David Liu, Hong Redfern, Jessica Zhu, Liya Colon-Carmona, Adan Univ Massachusetts Dept Biol Boston MA 02125 USA

The use of in vivo biosensors to acquire environmental pollution data is an emerging and promising paradigm. One major challenge is the identification of highly specific biomarkers that selectively report exposure to a target pollutant, while remaining quiescent under a diverse set of other, often unknown, environmental conditions. This study hypothesized that a microarray data mining approach can identify highly specific biomarkers, and, that the robustness property can generalize to unforeseen environmental conditions. Starting with Arabidopsis thaliana microarray data measuring responses to a variety of treatments, the study used the top scoring pair (TSP) algorithm to identify mRNA transcripts that respond uniquely to phenanthrene, a model polycyclic aromatic hydrocarbon. Subsequent in silico analysis with a larger set of microarray data indicated that the biomarkers remained robust under new conditions. Finally, in vivo experiments were performed with unforeseen conditions that mimic phenanthrene stress, and the biomarkers were assayed using qRT-PCR In these experiments, the biomarkers always responded positively to phenanthrene, and never responded to the unforeseen conditions, thereby supporting the hypotheses. This data mining approach requires only microarray or next-generation RNA-seq data, and, in principle, can be applied to arbitrary biomonitoring organisms and chemical exposures.

关键词： Data mining Biological monitoring Arabidopsis thaliana Research methodology Biochemical markers Polymerase chain reaction microarray technology Environmental monitoring

来源：评论

学校读者我要写书评

暂无评论

Gravimetric Analysis of the Adsorption and Desorption of CO₂ on Amine-Functionalized Mesoporous Silica Mounted on a Microcantilever Array

引用

ENVIRONMENTAL SCIENCE & technology 2011年第13期45卷 5704-5709页

作者： Lee, Dongkyu Jin, Yusung Jung, Namchul Lee, Jaehyuk Lee, Jinwoo Jeong, Yong Shik Jeon, Sangmin Pohang Univ Sci & Technol POSTECH Dept Chem Engn Pohang South Korea POSCO Environm & Energy Planning Dept Seoul South Korea

The kinetics of CO2 adsorption and desorption over amine-functionalized mesoporous silica were investigated using silicon microcantilever arrays. Three types of mesoporous silica with different pore sizes were synthesized and functionalized with a variety of amine molecules. After depositing the silica sorbents onto the free end of each cantilever in an array, mass changes due to the adsorption and desorption of CO2 were determined in situ with picogram sensitivity by measuring variations in the cantilever frequencies. The adsorption and desorption kinetics were found to be diffusion-controlled, and the kinetics were accelerated by increasing the temperature and pore size. The activation energies for adsorption and desorption of CO2 were determined from Arrhenius plots.

关键词： Gravimetric analysis Carbon dioxide adsorption Desorption (Chemistry) Effect of temperature on chemical kinetics Activation (Chemistry) Arrhenius equation Cantilevers microarray technology

来源：评论

学校读者我要写书评

暂无评论

Transcriptome Analysis of an Antibiotic Downregulator Mutant and Synergistic Actinorhodin Stimulation via Disruption of a Precursor Flux Regulator in Streptomyces coelicolor

引用

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2011年第5期77卷 1872-1877页

作者： Kim, Seon-Hye Lee, Han-Na Kim, Hye-Jin Kim, Eung-Soo Inha Univ Dept Biol Engn Inchon 402751 South Korea

Through microarray analysis of an antibiotic-downregulator-deleted Streptomyces coelicolor Delta wblA Delta SCO1712 mutant, 28 wblA- and SCO1712-dependent genes were identified and characterized. Among 14 wblA- and SCO1712-independent genes, a carbon flux regulating 6-phosphofructokinase SCO5426 was additionally disrupted in the Delta wblA Delta SCO1712 mutant and further stimulated actinorhodin production in S. coelicolor, implying that both regulatory and precursor flux pathways could be synergistically optimized for antibiotic production.

关键词： ANTIBIOTICS assay STREPTOMYCES coelicolor GENES microarray technology DNA microarrays MOLECULAR genetics

来源：评论

学校读者我要写书评

暂无评论

Multiplexed Method to Calibrate and Quantitate Fluorescence Signal for Allergen-Specific IgE

引用

ANALYTICAL CHEMISTRY 2011年第24期83卷 9485-9491页

作者： Monroe, Margo R. Reddington, Alexander P. Collins, Austin D. LaBoda, Craig Cretich, Marina Chiari, Marcella Little, Frederic F. Uenlue, M. Selim Boston Univ Dept Biomed Engn Boston MA 02215 USA Boston Univ Dept Elect & Comp Engn Boston MA 02215 USA CNR ICRM Milan Italy Boston Univ Sch Med Ctr Pulm Boston MA 02118 USA Boston Univ Dept Phys Boston MA 02215 USA

Using a microarray platform for allergy diagnosis allows for testing of specific IgE sensitivity to a multitude of allergens, while requiring only small volumes of serum. However, variation of probe immobilization on microarrays hinders the ability to make quantitative, assertive, and statistically relevant conclusions necessary in immunodiagnostics. To address this problem, we have developed a calibrated, inexpensive, multiplexed, and rapid protein microarray method that directly correlates surface probe density to captured labeled secondary antibody in clinical samples. We have identified three major technological advantages of our calibrated fluorescence enhancement (CaFE) technique: (i) a significant increase in fluorescence emission over a broad range of fluorophores on a layered substrate optimized specifically for fluorescence;(ii) a method to perform label-free quantification of the probes in each spot while maintaining fluorescence enhancement for a particular fluorophore;and (iii) a calibrated, quantitative technique that combines fluorescence and label-free modalities to accurately measure probe density and bound target for a variety of antibody antigen pairs. In this paper, we establish the effectiveness of the CaFE method by presenting the strong linear dependence of the amount of bound protein to the resulting fluorescence signal of secondary antibody for IgG, beta-lactoglobulin, and allergen-specific IgEs to Ara h 1 (peanut major allergen) and Phl p 1 (timothy grass major allergen) in human serum.

关键词： FLUORESCENCE ALLERGENS IMMUNOGLOBULIN E microarray technology IMMUNODIAGNOSIS

来源：评论

学校读者我要写书评

暂无评论

Large-scale plasmonic microarrays for label-free high-throughput screening

引用

LAB ON A CHIP 2011年第21期11卷 3596-3602页

作者： Chang, Tsung-Yao Huang, Min Yanik, Ahmet Ali Tsai, Hsin-Yu Shi, Peng Aksu, Serap Yanik, Mehmet Fatih Altug, Hatice MIT Dept Elect Engn & Comp Sci Cambridge MA 02139 USA Boston Univ Dept Elect & Comp Engn Boston MA 02215 USA MIT Dept Biol Engn Cambridge MA 02139 USA

microarrays allowing simultaneous analysis of thousands of parameters can significantly accelerate screening of large libraries of pharmaceutical compounds and biomolecular interactions. For large-scale studies on diverse biomedical samples, reliable, label-free, and high-content microarrays are needed. In this work, using large-area plasmonic nanohole arrays, we demonstrate for the first time a large-scale label-free microarray technology with over one million sensors on a single microscope slide. A dual-color filter imaging method is introduced to dramatically increase the accuracy, reliability, and signal-to-noise ratio of the sensors in a highly multiplexed manner. We used our technology to quantitatively measure protein-protein interactions. Our platform, which is highly compatible with the current microarray scanning systems can enable a powerful screening technology and facilitate diagnosis and treatment of diseases.

关键词： microarray technology large sizes Scanning systems microarray Sensors Microscope slide Protein-Protein Interaction

来源：评论

学校读者我要写书评

暂无评论

Detection, Isolation, and Characterization of Acidophilic Methanotrophs from Sphagnum Mosses

引用

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2011年第16期77卷 5643-5654页

作者： Kip, Nardy Ouyang, Wenjing van Winden, Julia Raghoebarsing, Ashna van Niftrik, Laura Pol, Arjan Pan, Yao Bodrossy, Levente van Donselaar, Elly G. Reichart, Gert-Jan Jetten, Mike S. M. Damste, Jaap S. Sinninghe den Camp, Huub J. M. Op Radboud Univ Nijmegen Dept Microbiol IWWR NL-6525 AJ Nijmegen Netherlands Univ Utrecht Fac Geosci Dept Earth Sci NL-3508 TA Utrecht Netherlands Univ Utrecht NL-3584 CH Utrecht Netherlands Austrian Inst Technol Dept Bioresources A-2444 Seibersdorf Austria Royal Netherlands Inst Sea Res NIOZ Dept Marine Organ Biogeochem NL-1790 AB Den Burg Netherlands

Sphagnum peatlands are important ecosystems in the methane cycle. Methane-oxidizing bacteria in these ecosystems serve as a methane filter and limit methane emissions. Yet little is known about the diversity and identity of the methanotrophs present in and on Sphagnum mosses of peatlands, and only a few isolates are known. The methanotrophic community in Sphagnum mosses, originating from a Dutch peat bog, was investigated using a pmoA microarray. A high biodiversity of both gamma- and alphaproteobacterial methanotrophs was found. With Sphagnum mosses as the inoculum, alpha-and gammaproteobacterial acidophilic methanotrophs were isolated using established and newly designed media. The 16S rRNA, pmoA, pxmA, and mmoX gene sequences showed that the alphaproteobacterial isolates belonged to the Methylocystis and Methylosinus genera. The Methylosinus species isolated are the first acid-tolerant members of this genus. Of the acidophilic gammaproteobacterial strains isolated, strain M5 was affiliated with the Methylomonas genus, and the other strain, M200, may represent a novel genus, most closely related to the genera Methylosoma and Methylovulum. So far, no acidophilic or acid-tolerant methanotrophs in the Gammaproteobacteria class are known. All strains showed the typical features of either type I or II methanotrophs and are, to the best of our knowledge, the first isolated (acidophilic or acid-tolerant) methanotrophs from Sphagnum mosses.

关键词： PEAT mosses METHANOTROPHS PEAT bogs METHANE microarray technology RNA

来源：评论

学校读者我要写书评

暂无评论

Physiological and Transcriptional Characterization of Persistent and Nonpersistent Listeria monocytogenes Isolates

引用

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2011年第18期77卷 6559-6569页

作者： Fox, Edward M. Leonard, Nola Jordan, Kieran Teagasc Food Res Ctr Fermoy Cork Ireland Univ Coll Dublin Sch Agr Food Sci & Vet Med Vet Sci Ctr Dublin 4 Ireland

This study aimed to characterize physiological differences between persistent and presumed nonpersistent Listeria monocytogenes strains isolated at processing facilities and to investigate the molecular basis for this by transcriptomic sequencing. Full metabolic profiles of two strains, one persistent and one nonpersistent, were initially screened using Biolog's Phenotype microarray (PM) technology. Based on these results, in which major differences from selected antimicrobial agents were detected, another persistent strain and two nonpersistent strains were characterized using two antimicrobial PMs. Resistance to quaternary ammonium compounds (QACs) was shown to be higher among persistent strains. Growth of persistent and nonpersistent strains in various concentrations of the QACs benzethonium chloride (BZT) and cetylpyridinium chloride (CPC) was determined. Transcriptomic sequencing of a persistent and a presumed nonpersistent strain was performed to compare gene expression among these strains in the presence and absence of BZT. Two strains, designated "frequent persisters" because they were the most frequently isolated at the processing facility, showed overall higher resistance to QACs. Transcriptome analysis showed that BZT induced a complex peptidoglycan (PG) biosynthesis response, which may play a key role in BZT resistance. Comparison of persistent and nonpersistent strains indicated that transcription of many genes was upregulated among persistent strains. This included three gene operons: pdu, cob-cbi, and eut. These genes may play a role in the persistence of L. monocytogenes outside the human host.

关键词： LISTERIA monocytogenes microarray technology ANTI-infective agents AMMONIUM compounds CETYLPYRIDINIUM chloride

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：