A new simple, rapid and sensitive reversed-phase liquid chromatographic method was developed and validated for the simultaneous determination of methocarbamol (MET) and aspirin (ASP) in their combined dosage form. The...
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A new simple, rapid and sensitive reversed-phase liquid chromatographic method was developed and validated for the simultaneous determination of methocarbamol (MET) and aspirin (ASP) in their combined dosage form. The separation of these compounds was achieved within 6.0min on a CLC Shim-pack C8 column (250x4.6mm, 5 mu m particle size) using isocratic mobile phase consisting of acetonitrile and 0.02 M dihydrogenphosphate buffer (30:70, v/v) at pH=5.0. The analysis was performed at a flow rate of 1.0mL/min with fluorescence detection at 277/313nm for MET and 298/410nm for ASP using real-time programming. The selectivity, linearity of calibration, accuracy, inter- and intra-day precision and recovery were examined as parts of the method validation. The concentration-response relationship was linear over concentration ranges of 0.02-0.20 and 0.02-0.40 mu g/mL for MET and ASP, respectively, with a limit of detection of 6 and 32ng/mL for MET and ASP, respectively. The proposed method was successfully applied for the analysis of both MET and ASP in prepared tablets with average recoveries of 99.88 +/- 0.65% for MET and 100.44 +/- 0.78% for ASP. The results were favourably compared to those obtained by a reference method. Copyright (c) 2012 John Wiley & Sons, Ltd.
Alfuzosin and tamsulosin are recently co-administrated with vardenafil to treat symptoms of benign prostatic hyperplasia and erectile dysfunction. A highly sensitive and simple liquid chromatographic method was develo...
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Alfuzosin and tamsulosin are recently co-administrated with vardenafil to treat symptoms of benign prostatic hyperplasia and erectile dysfunction. A highly sensitive and simple liquid chromatographic method was developed and validated for the simultaneous determination of the three drugs using moxifloxacin as an internal standard. lsocratic separation was achieved within 7.0 min using phenyl-hexyl column (250 :, 4.6 mm i.d.) and a mobile phase composed of acetonitrile/0.25% phosphoric acid (30:70, v/v) at pH 3.0. The analysis was performed at a flow rate of 1.2 mL/min with fluorescence detection at 246/450 nm for Alfuzosin and vardenafil, and 226/322 nm for tamsulosin using time programming technique. The proposed method was linear over the concentration ranges of 5.0-50.0 ng/mL, 10.0-200.0 ng/mL and 20.0-400.0 ng/mL for alfuzosin, vardenafil and tamsulosin, with limits of detection of 0.56 ng/mL, 0.98 ng/mL and 2.81 ng/mL in a respective order. The developed method was successfully applied to determine the studied drugs in dosage forms and human plasma samples and the results were satisfactory as revealed by statistical analysis of the data. (C) 2018 Academie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.
The paper describes the results of using a time and synchronization toolkit in a medical imaging application. The design and architectural principles of the toolkit are summarized. We then present the cineloop synchro...
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The new media types used in advance user interfaces and interactive systems introduce time as a significant variable. This paper addresses the architectural support and programming tools that should be provided to the...
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ISBN:
(纸本)0897915496
The new media types used in advance user interfaces and interactive systems introduce time as a significant variable. This paper addresses the architectural support and programming tools that should be provided to the programmer to manage the time dependencies. The approach considers that the basic models and programming paradigms adopted in the manipulation and management of time should be isomorphic with the spatial models used in existing graphical user *** paper describes the architectural principles of a toolkit designed to support the construction of user interfaces with temporal characteristics. The Ttoolkit is an extension of an existing graphical user interface toolkit, the Xt toolkit. Its design is presented and a sample application is described.
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