P>We present a new algorithm to analyse information content in images acquired using automated fluorescence microscopy. The algorithm belongs to the group of autofocusing methods, but differs from its predecessors ...
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P>We present a new algorithm to analyse information content in images acquired using automated fluorescence microscopy. The algorithm belongs to the group of autofocusing methods, but differs from its predecessors in that it can handle thick specimens and operate also in confocal mode. It measures the information content in images using a 'content function', which is essentially the same concept as a focus function. Unlike previously presented algorithms, this algorithm tries to find all significant axial positions in cases where the content function applied to real data is not unimodal, which is often the case. This requirement precludes using algorithms that rely on unimodality. Moreover, choosing a content function requires careful consideration, because some functions suppress local maxima. First, we test 19 content functions and evaluate their ability to show local maxima clearly. The results show that only six content functions succeed. To save time, the acquisition procedure needs to vary the step size adaptively, because a wide range of possible axial positions has to be passed so as not to miss a local maximum. The algorithm therefore has to assess the steepness of the content function online so that it can decide to use a bigger or smaller step size to acquire the next image. Therefore, the algorithm needs to know about typical behaviour of content functions. We show that for normalized variance, one of the most promising content functions, this knowledge can be obtained after normalizing with respect to the theoretical maximum of this function, and using hierarchical clustering. The resulting algorithm is more reliable and efficient than a simple procedure with constant steps.
Based on the theory of Phase Gradient autofocus on airborne SAP, an improved algorithm for reducing the Phase Gradient autofocus computation is provided in this paper. According to the image contrast criterion, some i...
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ISBN:
(纸本)9780769535579
Based on the theory of Phase Gradient autofocus on airborne SAP, an improved algorithm for reducing the Phase Gradient autofocus computation is provided in this paper. According to the image contrast criterion, some isolated stronger scatters for estimation phase errors are chosen, and then a new way to select windows width is developed, which can accelerate the convergence of the autofocus. Point target simulation is carried out, which verify the feasibility and the validity of this algorithm. The present algorithm can estimate discretional. rank phase errors and doesn't require strong reflection point, which is up to accuracy. After decreasing the computation of this algorithm, it is more suitable for Real-time processing.
In order to meet the high-precision measurement requirement of tested objects with tiny dimension and complex shape, a miniature five-coordinate image measuring machine, which contains three translational axes and two...
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ISBN:
(纸本)9783037852828
In order to meet the high-precision measurement requirement of tested objects with tiny dimension and complex shape, a miniature five-coordinate image measuring machine, which contains three translational axes and two rotational axes, is designed and built. This machine can inspect the objects from multi-orientation and multi-posture in non-contact way. As an indispensable step of image measurement, the autofocus effect has a direct influence on the following image quality and measurement accuracy. In this paper, the composition and working principle of autofocus system are presented and discussed. Meanwhile, the path planning method for autofocus system is introduced and an autofocus method based on evaluation function of mean square deviation is proposed and discussed in detail. Finally, the experiments demonstrate the effectiveness of the method and the accuracy of autofocus system.
The phase perturbations due to propagation effects can destroy the high resolution imagery of Synthetic Aperture Imaging Ladar (SAIL). Some autofocus algorithms for Synthetic Aperture Radar (SAR) were developed and im...
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ISBN:
(纸本)9780819487452
The phase perturbations due to propagation effects can destroy the high resolution imagery of Synthetic Aperture Imaging Ladar (SAIL). Some autofocus algorithms for Synthetic Aperture Radar (SAR) were developed and implemented. Phase Gradient algorithm (PGA) is a well-known one for its robustness and wide application, and Phase Curvature algorithm (PCA) as a similar algorithm expands its applied field to strip map mode. In this paper the autofocus algorithms utilized in optical frequency domain are proposed, including optical PGA and PCA respectively implemented in spotlight and strip map mode. Firstly, the mathematical flows of optical PGA and PCA in SAIL are derived. The simulations model of the airborne SAIL is established, and the compensation simulations of the synthetic aperture laser images corrupted by the random errors, linear phase errors and quadratic phase errors are executed. The compensation effect and the cycle index of the simulation are discussed. The simulation results show that both the two optical autofocus algorithms are effective while the optical PGA outperforms the optical PCA, which keeps consistency with the theory.
autofocus method based on the analysis of image content information is investigated to reduce the alignment error resulting from mark positioning uncertainty due to defocus in microstructure layered fabrication proces...
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autofocus method based on the analysis of image content information is investigated to reduce the alignment error resulting from mark positioning uncertainty due to defocus in microstructure layered fabrication process based on multilevel imprint lithography. The applicability of several autofocus functions to the alignment mark images is evaluated concerning their uniformity, sharpness near peak, reliability and measure computation efficiency and the most suitable one based on power spectrum in frequency domain (PSFD) is adopted. To solve the problem of too much computation amount needed in PSFD algorithm, the strategy of interested region detection and effective image reconstruction is proposed and the algorithm efficiency is improved. The test results show that the computation time is reduced from 0.316 s to 0.023 s under the same conditions while the other merits of the function are preserved, which indicates that the modified algorithm can meet the mark image autofocusing requirements in response time, accuracy and robustness. The alignment error due to defocus which is about 0.5 μm indicated by experimental results can be reduced or eliminated by the autofocusing implementation.
Due to consumers' demand for faster picture shot time in the rapidly expanding digital still camera market, it is of importance to address the real-time implementation issues in the development of passive automati...
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Due to consumers' demand for faster picture shot time in the rapidly expanding digital still camera market, it is of importance to address the real-time implementation issues in the development of passive automatic focusing for digital still cameras. This article discusses such real-time implementation issues that are often overlooked when designing passive contrast sensing automatic focusing on digital still camera processors. Specifically, algorithmic design tradeoffs between automatic focusing speed, accuracy, and power consumption, are addressed. A sample implementation and its performance results on an actual digital still camera hardware platform powered by the Texas Instruments TMS320DM270 processor are presented to further convey these real-time implementation issues.
The phase error estimated by phase gradient autofocus(PGA) is not based on a finite order polynomial mode, so PGA has a good autofocus property for arbitrary order phase error and is fit for high resolution airborne S...
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The phase error estimated by phase gradient autofocus(PGA) is not based on a finite order polynomial mode, so PGA has a good autofocus property for arbitrary order phase error and is fit for high resolution airborne SAR. But PGA has two shortcomings: first, it has a worse estimation property for fast changing phase error; second, there exists a section of linear phase in the phase error estimated by this algorithm. This paper introduces the idea of rank one phase estimate (ROPE) autofocus technique, and improves PGA. The improved PGA(IPGA) can successfully overcome both these shortcomings of PGA.
This paper presents an improved rank one phase error estimate(ROPE) autofocus technique. Compared with ROPE,the improved algorithm(IROPE) has two distinct ***,its model is more actual,so its property of robustness is ...
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This paper presents an improved rank one phase error estimate(ROPE) autofocus technique. Compared with ROPE,the improved algorithm(IROPE) has two distinct ***,its model is more actual,so its property of robustness is ***,at the beginning of it's iteration process,the initialized valves are set up *** approximation is more exact as compared to the blind initialization of *** estimator of phase error is maximum-likelihood (ML) one,and,under some special conditions, its estimator is the same as mat of phase gradient autofocus(PGA),which is linear unbiased minimum variation(LUMV) *** ROPE and PGA are subsets of IROPE.
A subaperture autofocus algorithm for synthetic aperture radar (SAR) partitions range-compressed phase-history data collected over a full aperture into equal-width subapertures. Application of a one-dimensional Fourie...
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A subaperture autofocus algorithm for synthetic aperture radar (SAR) partitions range-compressed phase-history data collected over a full aperture into equal-width subapertures. Application of a one-dimensional Fourier transform to each range bin converts each subaperture data set into a full-scene image (map). Any linear phase difference, or phase ramp, between a pair of subapertures expresses itself as cross-range drift in their maps. A traditional autofocus algorithm fits a polynomial to inferred equal-width phase ramps. If the true phase error function contains significant high-order components, then polynomial regression generates a poor estimate of the phase error function. Instead of fitting a polynomial, we fit a sinusoidal function through the inferred phase ramps. An example with a degraded SAR image shows how a sinusoidal correction improves image quality. We compare lower bounds on mean squared error (MSE) for polynomial and sinusoidal parameterizations. Sinusoidal parameterization reduces MSE significantly for model orders greater than five.
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