检索结果-内蒙古大学图书馆

The role of interactions of long non-coding rnas and heterogeneous nuclear ribonucleoproteins in regulating cellular functions

引用

BIOCHEMICAL JOUrnaL 2017年第17期474卷 2925-2935页

作者： Sun, Xinghui Ali, Mohamed Sham Shihabudeen Haider Moran, Matthew Univ Nebraska Dept Biochem Lincoln NE 68588 USA Univ Nebraska Nebraska Ctr Prevent Obes Dis Dietary Mol Lincoln NE 68583 USA

Long non-coding rnas (lncrnas) are emerging as critical regulators of various biological processes and human diseases. The mechanisms of action involve their interactions with proteins, rna and genomic DNA. Most lncrnas display strong nuclear localization. Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a large family of rna-binding proteins that are important for multiple aspects of nucleic acid metabolism. hnRNPs are also predominantly expressed in the nucleus. This review discusses the interactions of lncrnas and hnRNPs in regulating gene expression at transcriptional and post-transcriptional levels or by changing genomic structure, highlighting their involvements in glucose and lipid metabolism, immune response, DNA damage response, and other cellular functions. Toward the end, several techniques that are used to identify lncrna binding partners are summarized. There are still many questions that need to be answered in this relatively new research area, which might provide novel targets to control the biological outputs of cells in response to different stimuli.

关键词： epigenetics eukaryotic gene expression large intervening non-coding rna metabolism ribonucleoproteins signalling

来源：评论

学校读者我要写书评

暂无评论

Association between polymorphism in the promoter region of lncrna GAS5 and the risk of colorectal cancer

引用

BIOSCIENCE REPORTS 2019年第4期39卷 1-11页

作者： Wang, Yajie Wu, Shenshen Yang, Xi Li, Xiaobo Chen, Rui Southeast Univ Sch Publ Hlth Minist Educ Key Lab Environm Med Engn Nanjing 210009 Jiangsu Peoples R China

The growth arrest special 5 (GAS5), as a research hotspot of long noncoding rnas (lncrnas), has been reported to be associated with colorectal cancer (CRC). However, the association between polymorphisms in GAS5 and the risk of CRC was not clear. In the present study, a case-control study in 1078 CRC patients and 1175 matched healthy controls was performed to evaluate the association between the potential functional genetic variants in GAS5 and the risk of CRC. PCR-TaqMan, qPCR, dual-luciferase assay, electrophoretic mobility shift assay (EMSA), flow cytometry, migration and invasion assays were performed to evaluate the function of polymorphism. Results showed that subjects carrying the rs55829688 CT/TT genotypes had a significantly higher risk of CRC when compared with the CC genotype. Further qPCR assay confirmed that the CRC tissues with rs55829688 CT/TT genotypes had a higher GAS5 mrna expression level. The dual-luciferase assay, qPCR and EMSA assay revealed that rs55829688 T>C polymorphism could decrease the expression level of GAS5 by impacting the binding ability of the transcription factor Yin Yang-1 (YY1) to the GAS5 promoter region. The expression of apoptosis-related proteins were detected by Western blot. Further, flow cytometry, migration, and invasion experiments showed that GAS5 repressed apoptosis and increased invasion and migration capability of CRC cells. Taken together, our findings provided evidence that the rs55829688 variant in the GAS5 promoter was associated with the risk of CRC and decreased expression of GAS5 by affecting the binding affinity of the transcription factors YY1 to GAS5.

关键词： GAS5 colorectal cancer large intervening non-coding rna single nucleotide polymorphisms

来源：评论

学校读者我要写书评

暂无评论

FEZF1-AS1 functions as an oncogenic lncrna in retinoblastoma

引用

BIOSCIENCE REPORTS 2019年第5期39卷 BSR20190754.-BSR20190754.页

作者： Quan, Lian-Jiao Wang, Wen-Jun Qingyang Peoples Hosp Dept Ophthalmol Qingyan 745000 Gansu Peoples R China Xian 4 Hosp Dept Ophthalmol Xian 710004 Shaanxi Peoples R China Shaanxi Ophthalm Med Ctr Dept Ophthalmol Xian 710004 Shaanxi Peoples R China Xi An Jiao Tong Univ Sch Med Affiliated Guangren Hosp Dept Ophthalmol Xian 710004 Shaanxi Peoples R China

Long non-coding rna (lncrna) FEZF1 antisense rna 1 (FEZF1-AS1) has been shown to be up-regulated in tumor tissues and cells, and exerts oncogenic effects on various types of malignancies. However, the expression and function of FEZF1-AS1 was still fully unclear in retinoblastoma. The purpose of our study was to investigate the expression and clinical value of FEZF1-AS1 in retinoblastoma patients, and explore the effect of FEZF1-AS1 on retinoblastoma cell proliferation, migration and invasion. In our results, levels of FEZF1-AS1 expression were elevated in retinoblastoma tissue specimens and cell lines compared with adjacent normal retina tissue specimens and human retinal pigment epithelial cell line, respectively. The correlation analysis indicated that high FEZF1-AS1 expression was significantly correlated with present choroidal invasion and optic nerve invasion. Survival analysis suggested that retinoblastoma patients in high FEZF1-AS1 expression group had obviously short disease-free survival (DFS) compared with retinoblastoma patients in low FEZF1-AS1 expression group, and high FEZF1-AS1 expression was an independent unfavorable prognostic factor for DFS in retinoblastoma patients. Loss-of-function study indicated silencing FEZF1-AS1 expression inhibited retinoblastoma cell proliferation, invasion and migration. In conclusion, FEZF1-AS1 functions as an oncogenic lncrna in retinoblastoma.

关键词： biomarkers cancer FEZF1-AS1 large intervening non-coding rna retinoblastoma

来源：评论

学校读者我要写书评

暂无评论

LINC00675 is a prognostic factor and regulates cell proliferation, migration and invasion in glioma

引用

BIOSCIENCE REPORTS 2018年第5期38卷 BSR20181039-BSR20181039页

作者： Li, Zhibing Li, Yijian Wang, Qibai Shanxi Prov Tumor Hosp Dept Neurol Taiyuan 030003 Shanxi Peoples R China Tradit Chinese Med Hosp Juye Cty Dept Surg 2 Heze 274900 Shandong Peoples R China Jiangbei Dist Peoples Hosp Hosp Chongqing Red Cross Soc Dept Neurol Chongqing 400025 Peoples R China

LINC00675 has been suggested to be dysregulated in gastric cancer, colorectal cancer and pancreatic cancer. However, the expression status and biological function of LINC00675 in glioma were still unknown. Thus, we reported LINC00675 was overexpressed in glioma tissues and cell lines, and positively associated with advanced WHO grade, large tumor size and poor prognosis. Moreover, univariate and multivariate analyses suggested that high-expression of LINC00675 was an independent unfavorable prognostic predictor for glioma. In addition, levels of LINC00675 expression were positively correlated with TRIP6 mrna and protein expressions. The in vitro experiment showed that silencing of LINC00675 inhibits glioma cell proliferation, migration and invasion through regulating TRIP6. In conclusion, LINC00675 acts as a tumor promoter in glioma progression.

关键词： biomarkers cancer glioma LINC00675 large intervening non-coding rna prognosis

来源：评论

学校读者我要写书评

暂无评论

Regulatory effects of lncrnas and mirnas on autophagy in malignant tumorigenesis

引用

BIOSCIENCE REPORTS 2018年第5期38卷 BSR20180516-BSR20180516页

作者： Yin, Qingqing Feng, Wei Shen, Xianjuan Ju, Shaoqing Nantong Univ Affiliated Hosp Ctr Lab Med Nantong Peoples R China Nantong Univ Affiliated Hosp Surg Comprehens Lab Nantong Peoples R China

Autophagy is an important process in endogenous substrate degradation by lysosomes within cells, with a degree of evolutionary conservation. Like apoptosis and cell senescence, cell autophagy is a very important biological phenomenon involving the development and growth of biological processes. Abnormal autophagy may lead to tumorigenesis. In recent years, increasing studies have demonstrated that long non-coding rnas (lncrnas) and mirnas can regulate cell autophagy by modulating targetting gene expression. In this review, we will provide an overview of lncrnas and mirnas in autophagy modulation and new insights into the underlying mechanisms, as well as their potential utilization in disease diagnosis, prognosis, and therapy.

关键词： autophagy large intervening non-coding rna microrna oncogenesis gene therapy

来源：评论

学校读者我要写书评

暂无评论

Altered expression of long non-coding rna GAS5 in digestive tumors

引用

BIOSCIENCE REPORTS 2019年第1期39卷 BSR20180789-BSR20180789页

作者： Lu, Shounan Su, Zhilei Fu, Wen Cui, Zhankun Jiang, Xingming Tai, Sheng Harbin Med Univ Affiliated Hosp 2 Dept Hepatopancreatobiliary Surg Harbin 150086 Heilongjiang Peoples R China

Cancer has become one of the most important diseases that affect human health and life. The effects of cancer in the digestive system are particularly prominent. Recently, long non-coding rna (lncrna) has attracted the attention of more and more researchers and has become an emerging field of gene research. The lncrna growth arrest-specific 5 (GAS5) is a novel lncrna that has attracted the attention of researchers in recent years and plays an important role in the development of tumors, especially in digestive system tumors. GAS5 was first identified in a mouse cDNA library. It was generally considered that it has the role of tumor suppressor genes, but there are still studies that have a certain ability to promote cancer. Furthermore, the 5-bp indel polymorphism (rs145204276) in the GAS5 promoter region also has a carcinogenic effect. The discovery of GAS5 and in-depth study of single nucleotide polymorphism (SNP) mechanism can provide a new way for the prevention and treatment of digestive system tumors.

关键词： digestive system tumor GSA5 large intervening non-coding rna single nucleotide polymorphisms

来源：评论

学校读者我要写书评

暂无评论

The long noncoding rna Meg3 mediates TLR4-induced inflammation in experimental obstructive nephropathy

引用

CLINICAL SCIENCE 2023年第5期137卷 317-331页

作者： Yiu, Wai Han Lok, Sarah W. Y. Xue, Rui Chen, Jiaoyi Lai, Kar Neng Lan, Hui Yao Tang, Sydney C. W. Univ Hong Kong Queen Mary Hosp Dept Med Hong Kong Peoples R China Chinese Univ Hong Kong Dept Med & Therapeut Hong Kong Peoples R China Chinese Univ Hong Kong Li Ka Shing Inst Hlth Sci Hong Kong Peoples R China

Kidney inflammation contributes to the progression of chronic kidney disease (CKD). Modulation of Toll-like receptor 4 (TLR4) signaling is a potential therapeutic strategy for this pathology, but the regulatory mechanisms of TLR4 signaling in kidney tubular inflammation remains unclear. Here, we demonstrated that tubule-specific deletion of TLR4 in mice conferred protection against obstruction-induced kidney injury, with reduction in inflammatory cytokine production, macrophage infiltration and kidney fibrosis. Transcriptome analysis revealed a marked down-regulation of long noncoding rna (lncrna) Meg3 in the obstructed kidney from tubule-specific TLR4 knockout mice compared with wild-type control. Meg3 was also induced by lipopolysaccharide in tubular epithelial cells via a p53-dependent signaling pathway. Silencing of Meg3 suppressed LPS-induced cytokine production of CCL-2 and CXCL-2 and the activation of p38 MAPK pathway in vitro and ameliorated kidney fibrosis in mice with obstructive nephropathy. Together, these findings identify a proinflammatory role of lncrna Meg3 in CKD and suggest a novel regulatory pathway in TLR4-driven inflammatory responses in tubular epithelial cells.

关键词： kidney fibrosis large intervening non-coding rna Meg3 TLR4 signaling tubular injury

来源：评论

学校读者我要写书评

暂无评论

LSINCT5 predicts unfavorable prognosis and exerts oncogenic function in osteosarcoma

引用

BIOSCIENCE REPORTS 2019年第5期39卷 BSR20190612.-BSR20190612.页

作者： He, Weidong Lu, Ming Xiao, Dongbo First Peoples Hosp Lianyungang Dept Joint Surg Lianyungang 222002 Jiangsu Peoples R China Med Sch Chinese PLA Dept Orthoped Beijing 100853 Peoples R China Ankang Cent Hosp Dept Orthoped Ankang 725000 Shaanxi Peoples R China

The dysregulated expression of LSINCT5 (long stress-induced non-coding transcript 5) has been found in various human tumors, and was generally related to cancer progression and unfavorable prognosis. Although the role of LSINCT5 in osteosarcoma was reported not long ago, the sample size of that study was limited. Our study presented more evidence about the clinical significance and biological function of LSINCT5 in osteosarcoma. In our results, we found LSINCT5 expression was increased in osteosarcoma tissue samples and cell lines, and high LSINCT5 expression was associated with advanced Enneking stage, large tumor size, high histological grade and present distant metastasis. Meanwhile, we observed high LSINCT5 expression was correlated with worse overall survival, and high LSINCT5 expression could be an independent poor predictor for overall survival in osteosarcoma cases. Moreover, we found inhibition of LSINCT5 expression suppressed cell proliferation, migration and invasion in vitro, and LSINCT5 overexpression dramatically facilitated cell proliferation, migration and invasion in vitro. In conclusion, our study suggests that LSINCT5 exerts oncogenic function in osteosarcoma cells, and may be a potential predictor for clinical outcome in osteosarcoma patients.

关键词： biomarker large intervening non-coding rna LSINCT5 osteosarcoma

来源：评论

学校读者我要写书评

暂无评论

Lncrna PFAR contributes to fibrogenesis in lung fibroblasts through competitively binding to miR-15a

引用

BIOSCIENCE REPORTS 2019年第7期39卷 BSR20190280.-BSR20190280.页

作者： Sun, Jian Su, Wei Zhao, Xiaoguang Shan, Tianjiao Jin, Tongzhu Guo, Yingying Li, Chao Li, Ruotong Zhou, Yuhong Shan, Hongli Sun, Xiaohan Liang, Haihai Harbin Med Univ Coll Pharm State Prov Key Labs Biomed Pharmaceut China Minist EducKey Lab Cardiovasc ResDept Pharmacol Harbin 150081 Heilongjiang Peoples R China Harbin Med Univ Heilongjiang Acad Med Sci Northern Translat Med Res & Cooperat Ctr Harbin 150081 Heilongjiang Peoples R China Harbin Med Univ Affiliated Hosp 2 Dept Pediat Harbin 150086 Heilongjiang Peoples R China

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, debilitating disease with unknown etiopathogenesis. Previous reports have reported that long non-coding rnas (lncrnas) were involved in various pathophysiological processes. However, the role of lncrnas in IPF has not been fully described. We aimed to explore the relationship between miR-15a and lncrna PFAR and its function in pulmonary fibrosis. Biological information analysis and luciferase were used to identify targeted binding of lncrna PFAR and miR-15a. Western blot, quantitative reverse transcription-PCR (qRT-PCR) and immunofluorescence staining were conducted to detect fibrosis-related factors. Fibroblasts proliferation were analyzed using 5-ethynyl-2'-deoxyuridine (EdU) staining and fibroblasts migration ability were measured using wound-healing scratch assay. We identified that lncrna PFAR has a binding site with miR-15a and luciferase reporter assays demonstrated their combinative relationship. Our results showed that silencing PFAR attenuated TGF-beta 1 induced fibrogenesis in primary lung fibroblasts. And miR-15a antagonized the function of PFAR and inhibited PFAR induced extracellular collagen deposition, fibroblasts proliferation, migration and differentiation. In conclusion, our results revealed that PFAR functions as a competitive endogenous rna (cerna) by acting as a sponge for miR-15a, revealing a potential regulatory network involving PFAR and miR-15a with a role in the modulation of YAP1-Twist expression. This mechanism may contribute to a better understanding of pulmonary fibrosis pathogenesis and treatment method.

关键词： fibroblasts large intervening non-coding rna microrna

来源：评论

学校读者我要写书评

暂无评论

The multifaceted actions of the lncrna H19 in cardiovascular biology and diseases

引用

CLINICAL SCIENCE 2022年第15期136卷 1157-1178页

作者： Busscher, Denise Boon, Reinier A. Juni, Rio P. Vrije Univ Amsterdam Dept Physiol Amsterdam UMC Boelelaan 1117 Amsterdam Netherlands Amsterdam Cardiovasc Sci Microcirculat Amsterdam Netherlands Goethe Univ Frankfurt Main Inst Cardiovasc Regenerat Ctr Mol Med Frankfurt Germany German Ctr Cardiovasc Res DZHK Partner site Frankfurt Rhein Main Frankfurt Germany

Cardiovascular diseases are the leading cause of death and debility worldwide. Various molecular mechanisms have been studied to better understand the development and progression of cardiovascular pathologies with hope to eradicate these diseases. With the advancement of the sequencing technology, it is revealed that the majority of our genome is non-coding. A growing body of literature demonstrates the critical role of long non-coding rnas (lncrnas) as epigenetic regulators of gene expression. Lncrnas can regulate cellular biological processes through various distinct molecular mechanisms. The abundance of lncrnas in the cardiovascular system indicates their significance in cardiovascular physiology and pathology. Lncrna H19, in particular, is a highly evolutionarily conserved lncrna that is enriched in cardiac and vascular tissue, underlining its importance in maintaining homeostasis of the cardiovascular system. In this review, we discuss the versatile function of H19 in various types of cardiovascular diseases. We highlight the current literature on H19 in the cardiovascular system and demonstrate how dysregulation of H19 induces the development of cardiovascular pathophysiology.

关键词： cardiovascular disease cardiovascular physiology large intervening non-coding rna lncrna H19 non-coding rna

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：