Determination of mercury species over solid waste from nature gas processing plants is a prerequisite for solid waste decontamination and mercury recycle. Temperature programmed decomposition and desorption method (TP...
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13C Nuclei bonded to bromine nuclei can undergo efficient scalar relaxation;the presence of nearly equal amounts of 79Br and 81Br isotopes can lead to observed "non-exponential" relaxation for the 13C nuclei.
13C Nuclei bonded to bromine nuclei can undergo efficient scalar relaxation;the presence of nearly equal amounts of 79Br and 81Br isotopes can lead to observed "non-exponential" relaxation for the 13C nuclei.
The authors analyzed the contribution to megakaryocyte colony formation in methylcellulose made by human plasma, serum, media conditioned by phytohemagglutinin (PHA) stimulated leukocytes (PHA-LCM), erythropoietin (EP...
The authors analyzed the contribution to megakaryocyte colony formation in methylcellulose made by human plasma, serum, media conditioned by phytohemagglutinin (PHA) stimulated leukocytes (PHA-LCM), erythropoietin (EPO) preparations and platelets. The culture system was used as a bioassay for megakaryocyte colony stimulating activity (Meg-CSA)in plasma samples of patients with perturbed megakaryocytopoiesis. Preparations of heparinized platelet-poor plasma yielded the most consistent results. Platelet-poor plasma of normal subjects will, at best, facilitate the occasional growth of small megakaryocyte colonies. Colony frequency and size are reproducibly enhanced in the presence of PHA-LCM as a source of exogenous Meg-CSA. Commercially available EPO preparations may vary in their content of activities that influence magakaryocyte colony formation. Addition of these preparations to cultures that contain plasma and PHA-LCM usually does not enhance colony formation. In contrast to platelet-poor plasma, platelet rich plasma and serum are less supportive of megakaryocyte colony growth. This loss of activity evidently may be related to the release of inhibitors by activated platelets, or may alternatively be caused by absorption of activities by platelets. Plasma samples from patients with megakaryocytopoietic dysfunction may contain components that promote colony formation without addition of PHA-LCM or EPO. This phenomenon is consistently observed for patients with severe aplastic anemia and for bone marrow transplant recipients after completion of their ablative preparative regimen.
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