目的中国是多种常见肝病的高发国家,肝脏疾病的研究对提高防治水平,保障人民健康有着重要的意义。为了全面收集、整理、共享和分析分散于现有数据库和文献资料中的肝脏生理病理学知识,该研究构建了人类肝脏疾病本体HuL DO并展开多方面应用。方法 HuL DO是参考疾病相关的多个权威术语集和肝脏病学专著而构建的,依据该本体中的疾病名称列表,从现有数据库中收集各种肝病的相关数据,以本体结构为数据组织框架构建肝病知识库,以本体为字典挖掘肝病与基因的关系。结果 HuL DO是目前最全面的肝病概念分类系统,以该本体为基础建立了一整套肝病知识的收集、挖掘和展示的工具和策略。结论 HuL DO是全面收集、高效分享和灵活分析各类肝病数据和知识的重要工具。肝病本体的下载地址是ftp://***/***%20Disease%20Ontology/。
Objective:To generate an h MSH2-gene knockdown lung cancer cell model with small RNA interference in NCI-H520 cell line,so as to verify the underlying mechanisms in h MSH2-mediated recognition and cytolyisis of lung c...
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Objective:To generate an h MSH2-gene knockdown lung cancer cell model with small RNA interference in NCI-H520 cell line,so as to verify the underlying mechanisms in h MSH2-mediated recognition and cytolyisis of lung cancer cells by Vγ9δ2 T ***:h MSH2 specific si RNAs were designed,synthesized and transfected into NCI-H520 *** knockdown efficiency was measured by q RT-PCR,Westernblot,flow cytometery and confocal microscopy 48 or 72 h after si RNAs ***:Transfection efficiency was up to 93% 6 h after si RNAs treatment.48 or 72 h after specific si RNAs transfection,si RNA duplex I and II respectively resulted in 97%,88% and 98%,97% reduction in h MSH2 m RNA expression when compared to mock *** h MSH2 protein expression was confirmed by Western *** surface expression of h MSH2 in si RNA-treated groups was revealed by flow cytometery and confocal ***:si RNA-h MSH2 gene knockdown lung cancer cell model was successfully generated in NCI-H520 cell line,providing a useful means to explore the recognition and cytolytic mechanism in Vγ9δ2 T cell-mediated anti-lung cancer innate immunity.
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