Glucokinase(GK;E.C.2.7.1.1),belonging to hexokinase family,is the liver enzyme that converts glucose into *** is an important first step in *** we report a cDNA encoding Erythroculter ilishaeformis GK using RT-PCR and...
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Glucokinase(GK;E.C.2.7.1.1),belonging to hexokinase family,is the liver enzyme that converts glucose into *** is an important first step in *** we report a cDNA encoding Erythroculter ilishaeformis GK using RT-PCR and *** cDNA was 2 054 bp with 63 bp 5′UTR,563 bp 3′UTR[excluding poly(A)] and 1 428 bp ORF,which encodes 476 amino acids and has a predicted mol wt of 53.74 *** high similarity between sequences of GK in *** and GK sequences from other vertebrates confirms that this sequence corresponds to a functional enzyme;The *** GK appears to possess the ATP-binding site and glucose-binding site,*** deduced amino acid sequence of *** GK shares 96%,87%,86% sequence identity with common carp,rainbow trout,gilthead seabream,respectively,shares79%,80% identity with clawed frog and human,*** phylogenetic analysis the deduced amino acid sequence of *** GK,together with GK sequences reported for 12 eukaryotic GK amino acid sequences,were aligned by Clustal W,version 1.6 and the PAUP algorithm was used to produce an unrooted *** tree shows *** GK gene has closely relatedness with other three fish species,and highly homologous to mammalian GK.
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