采用RT-PCR和cDNA末端快速扩增法(RACE)分离、克隆出鲇(Parasilurus asotus)垂体阿黑皮素原(proopiomelanocortin,POMC)基因全长cDNA序列,共1 099 bp,包括129 bp 5′非翻译区、648 bp阅读框以及322 bp 3′非翻译区,阅读框共编码215个氨...
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采用RT-PCR和cDNA末端快速扩增法(RACE)分离、克隆出鲇(Parasilurus asotus)垂体阿黑皮素原(proopiomelanocortin,POMC)基因全长cDNA序列,共1 099 bp,包括129 bp 5′非翻译区、648 bp阅读框以及322 bp 3′非翻译区,阅读框共编码215个氨基酸,POMC前体蛋白的结构主要由信号肽(Met1-Ala28)、N端多肽(Gln29-Asp102)、促肾上腺皮质激素(ACTH,Ser 105-Met144)、α-黑色素细胞刺激激素(α-MSH,Ser105-Val 117)、促肾上腺皮质激素样中叶肽(CLIP,Arg123-Met144)、γ-促脂解激素(γ-LPH,Glu145-Ser158)、β-黑色素细胞刺激激素(β-MSH,Asp161-Ser177)和β-内啡肽(β-EP,Tyr178-Leu215)构成。分子量计算值为24.66 kD,理论等电点为7.25。同源性分析表明鲇与斑点叉尾的相似性高达88%,其构成多肽中α-MSH、β-MSH、β-EP、CLIP保守性逐渐降低,而γ-MSH、连接肽(JP)、γ-LPH是变异比较大的区域,同时鲇POMC中不含有γ-MSH,连接肽和部分γ-LPH也不存在。生物信息学分析显示鲇POMC基因所编码的蛋白质以亲水性区域为主,具有丰富的B细胞抗原位点。定量分析结果表明POMC mRNA主要在垂体中高表达,在非垂体组织的脑中表达量最高,鳃、头肾、肝、脾、肌肉、皮肤等组织也有少量表达。
Enzyme analytical method was used to investigate the activities and distributions of protease, amylase, cellulase in straight intestine, middle intestine, inner intestine, digestive gland, stomach and crystalline from...
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Enzyme analytical method was used to investigate the activities and distributions of protease, amylase, cellulase in straight intestine, middle intestine, inner intestine, digestive gland, stomach and crystalline from three different styles of Hyriopsis cumingii. The morphology and structure of crystalline style were also examined by paraffin section, light microscopy, scanning and transmission electron microscopy. The results showed that the relationship among digestive enzymes activities in all the six kinds of digestive organs was amylase>cellulase>protease, and amylase activity was significantly higher than cellulase and protease. Both protease and amylase activities were significantly higher in crystalline style, while cellulase activities were higher in stomach and orstraight. In contrast to a marked rise in protease activity, the amylase and cellulase demonstrated pronounced decreasing trend accompanied by the increasing wet weight of Hyriopsis cumingii. Compared to the solid line of crystalline style under starvation, a food channel was formed when Hyriopsis cumingii was full, which circumgyrated in one direction to mill, separate, enwrap food and digested them finally.
Glucokinase(GK;E.C.2.7.1.1),belonging to hexokinase family,is the liver enzyme that converts glucose into *** is an important first step in *** we report a cDNA encoding Erythroculter ilishaeformis GK using RT-PCR and...
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Glucokinase(GK;E.C.2.7.1.1),belonging to hexokinase family,is the liver enzyme that converts glucose into *** is an important first step in *** we report a cDNA encoding Erythroculter ilishaeformis GK using RT-PCR and *** cDNA was 2 054 bp with 63 bp 5′UTR,563 bp 3′UTR[excluding poly(A)] and 1 428 bp ORF,which encodes 476 amino acids and has a predicted mol wt of 53.74 *** high similarity between sequences of GK in *** and GK sequences from other vertebrates confirms that this sequence corresponds to a functional enzyme;The *** GK appears to possess the ATP-binding site and glucose-binding site,*** deduced amino acid sequence of *** GK shares 96%,87%,86% sequence identity with common carp,rainbow trout,gilthead seabream,respectively,shares79%,80% identity with clawed frog and human,*** phylogenetic analysis the deduced amino acid sequence of *** GK,together with GK sequences reported for 12 eukaryotic GK amino acid sequences,were aligned by Clustal W,version 1.6 and the PAUP algorithm was used to produce an unrooted *** tree shows *** GK gene has closely relatedness with other three fish species,and highly homologous to mammalian GK.
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