目的研究细胞膜穿透性寡肽———八聚精氨酸(R8)输送包载siRNA的脂质体进入细胞能力的促进作用。方法将合成的R8与PEG-PE形成偶联物并定向定量地插入包载siRNA脂质体的外层膜上制成R8-liposom alsiRNA。荧光分光光度计测定脂质体中R8的含量,荧光倒置显微镜观察R8修饰的脂质体与常用转染试剂lipofectam ine 2000携带siRNA对小细胞肺癌NC I-H446的转染效果,MTT法检测R8-liposom al siRNA对NC I-H446细胞的生长抑制作用。结果R8未修饰的脂质体不能穿过细胞膜,而R8修饰的脂质体则能迅速进入细胞内,随着作用时间的延长逐渐定位在细胞浆和细胞核。R8修饰的脂质体介导hdm2-siRNA转染肿瘤细胞的效率和对肿瘤细胞的生长抑制作用明显强于lipofectam ine 2000。结论R8修饰的脂质体可以有效输送siRNA进入细胞并增强其生物学功能。
GAP分析是用来判别生物多样性保护的空白、遗漏区域(“gaps”)和需要采取保护行动的地区(conservation gap),它是一种评估区域生物多样性所有成分所处保护地位、被保护程度等的科学方法。强调最大限度地保护生物多样性的先决条件是通过构建保护区网络,不仅仅考虑生物多样性最高地区,应该使本地种和生态系统都得到保护。区域生态安全格局的评价不仅要考虑生物及其生境的保护,而且,更要考虑受损生态系统和破坏景观的恢复,即不仅考虑Conservation G ap,也要考虑R estoration G ap。因而,改进GAP方法可用于生态安全格局的分析与评价,且有望成为重要的研究手段。
The volatile compositions of Chinese Pine (Pinus tabulaeformis) are analyzed. The main turpentine volatiles are (R)-(+)-α-pinene, (S)-(-)-β-pinene, (S)-(+)-3-carene (the largest) which occupy more than 97% of the to...
详细信息
The volatile compositions of Chinese Pine (Pinus tabulaeformis) are analyzed. The main turpentine volatiles are (R)-(+)-α-pinene, (S)-(-)-β-pinene, (S)-(+)-3-carene (the largest) which occupy more than 97% of the total. There are also myrcene, (+)-limonene, camphene, terpinen and phellandrene. In the experiment of Electroantennograms(EAG), the male of red turpentine beetles are sensitive to (S)-(-)-α-pinene、limonene and (S)-(+)-3-carene; while the female of the red turpentine beetles are sensitive to (S)-(+)-3-carene、limonene、(S)-(-)-α-pinene and myrcene. The female are more sensitive to the volatiles than the male. There is much difference in response of Dendroctonus valens in Chinese pine compared to that in USA in ponderosa pine. The volatile difference of the two pine species and the environment condition may contribute to this change. Further study on the mechanism of the change is needed.
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