采用GoldeneyeTM20A试剂盒复合扩增180例重庆地区无血缘关系汉族个体的19个STR基因座。扩增产物经ABI3130序列分析仪进行检测,GeneMapper ID V3.2.1基因分析软件进行基因分析,得到基因分型图。总共检测出182个等位基因,其频率分布介于0....
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采用GoldeneyeTM20A试剂盒复合扩增180例重庆地区无血缘关系汉族个体的19个STR基因座。扩增产物经ABI3130序列分析仪进行检测,GeneMapper ID V3.2.1基因分析软件进行基因分析,得到基因分型图。总共检测出182个等位基因,其频率分布介于0.0028～0.4972。19个STR基因座的杂合度介于0 6611～0.9111,个体识别率介于0.8114～0.9882,匹配概率介于0.0118～0.1 886,多态信息含量介于0.6309～0.9190,累积个人识别率和累积多态信息含量均大于0.9999999999,累积匹配概率为3.7189×10^(-24),累积非父排除率为0.9999999964。说明该19个STR基因座具有比较高的多态性,是较理想的遗传标记,所得到的等位基因频率等数据可为重庆地区汉族人群的法医学个人识别、亲子鉴定及群体遗传学等研究提供基础数据。
Chronic allograft rejection,which is manifested as chronic allograft vasculopathy(CAV),continues to refrain the long-term success of small bowel transplantation(SBTx).The pathway mediated by the receptor for advanced ...
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Chronic allograft rejection,which is manifested as chronic allograft vasculopathy(CAV),continues to refrain the long-term success of small bowel transplantation(SBTx).The pathway mediated by the receptor for advanced glycation end products(RAGE) and its ligand,high mobility group box-1(HMGBl),may contribute to the pathogenesis of CAV,given that they were involved in the process of allograft rejection,n-3 polyunsaturated fatty acids(PUFAs),which has been discovered to attenuate CAV,may have potential impacts on this pathway. The present study investigated whether n-3 PUFAs attenuated CAV via the regulation of HMGB1-RAGE pathway in a chronic rejection model of rat *** revealed that the expression of HMGBl and RAGE was increased in CAV-bearing vessels as well as endothelial cells isolated from these *** administration of fish oil with high levels of n-3 PUFAs following SBTx significantly reduced the HMGBl and RAGE expression,which coincided with the amelioration of *** contrast,feeding of corn oil that contained low levels of n-3 PUFAs had no favorable effects on CAV development,and failed to decrease the HMGBl and RAGE *** results indicate that protective effects of n-3 PUFAs on allograft vessels exist via downregulation of HMGB1- RAGE pathway.
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