乙肝病毒X蛋白(Hepatitis B virus X protein,HBx)是乙肝病毒诱导肝癌进展的关键因子,其能够影响肝癌细胞周期和促进其迁移。已知趋化因子受体3(chemokine CXC motif receptor 3,CXCR3)在乙型肝炎病毒肝癌中发挥促进作用,目前尚不清楚CX...
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乙肝病毒X蛋白(Hepatitis B virus X protein,HBx)是乙肝病毒诱导肝癌进展的关键因子,其能够影响肝癌细胞周期和促进其迁移。已知趋化因子受体3(chemokine CXC motif receptor 3,CXCR3)在乙型肝炎病毒肝癌中发挥促进作用,目前尚不清楚CXCR3在HBx诱导的肝癌细胞中的作用。因此,本研究探讨下调CXCR3对HBx诱导的肝癌细胞周期进展和迁移作用及机制。肝癌细胞Huh7分成对照组,pcDNA组(转染阴性对照载体),pcDNAHBx组(转染HBx过表达载体),pcDNA-HBx+si-NC组(共转染HBx过表达载体、siRNA对照的),pcDNA-HBx+si-CXCR3组(共转染HBx过表达载体、CXCR3 siRNA的),pcDNA-HBx+si-CXCR3+Jagged1组(共转染HBx过表达载体、CXCR3 siRNA,Notch1信号激活剂Jagged1处理),测定细胞中HBx、CXCR3、神经性钙黏附素(Neural cadherin,N-cadherin)、基质金属蛋白酶-9(Matrix metalloprotease 9,MMP-9)、细胞周期蛋白D1(cyclin D1)、增殖细胞核抗原(PCNA)、基质金属蛋白酶-2(Matrix metalloprotease 2,MMP-2)、上皮性钙黏附素(Epithelical cadherin,Ecadherin)、波形蛋白(Vimentin)、Notch神经同源蛋白1前体(Neurogenic locus notch homolog protein 1 precusor,Notch1)、Hes1蛋白表达,检测细胞增殖、周期和迁移、侵袭情况。结果表明,与Control、pcDNA组比较,pcDNAHBx组肝癌细胞中HBx、CXCR3、Notch1、Hes1、Cyclin D1、PCNA、MMP-9、MMP-2、vimentin、N-cadherin蛋白表达量升高,细胞增殖活性、迁移数目、侵袭数目升高,细胞G0/G1期比例、E-cadherin蛋白表达量降低(P<0.05);与pcDNA-HBx+si-NC组比较,pcDNA-HBx+si-CXCR3组肝癌细胞中CXCR3、Notch1、Hes1、Cyclin D1、PCNA、MMP-9、MMP-2、vimentin、N-cadherin蛋白表达量降低,细胞增殖活性、迁移数目、侵袭数目降低,细胞G0/G1期比例、E-cadherin蛋白表达量升高(P<0.05);与pcDNA-HBx+si-CXCR3组比较,pcDNA-HBx+si-CXCR3+Jagged1组肝癌细胞Notch1、Hes1、Cyclin D1、PCNA、MMP-9、MMP-2、vimentin、N-cadherin蛋白表达量升高,细胞增殖活性、迁移数目、侵袭数目升高,细胞G0/G1期比例、E-cadherin蛋白表达量降低(P<0.05)。结果表明,下调CXCR3通过抑制Notch1信号减弱HBx促进肝癌细胞周期进展和迁移作用。本研究证明了下调CXCR3对HBx促进肝癌细胞周期进展、迁移、侵袭和EMT的作用,并首次发现了其作用机制与Notch1信号有关。
目的:构建伤口造口失禁专科护士能级评价方案,为该领域专科护士的能力评价提供参考。方法:运用文献分析、专家讨论及德尔菲法构建评价方案。于2020年9月随机选取全国213名伤口造口失禁专科护士为研究对象,收集其对评价方案各项指标的认可程度评分及本方案在专业领域中的适用性、可执行性与可推广性的综合评价数据。结果:两轮专家函询的积极系数均达到100%,专家权威系数为0.872,Kendall’s W值分别为0.252和0.331 (P均Objective: To construct a competency evaluation scheme for wound, ostomy, and continence specialist nurses, providing a reference for the capability assessment of nurses in this field. Methods: The evaluation scheme was constructed using literature analysis, expert discussions, and the Delphi method. In September 2020, 213 wound, ostomy, and continence specialist nurses were randomly selected nationwide as research subjects. Data were collected on their recognition degree scores for each indicator of the evaluation scheme, as well as comprehensive evaluation data on the scheme’s applicability, executability, and promotability in the professional field. Results: The positive coefficients of the two rounds of expert consultations were both 100%, the expert authority coefficient was 0.872, and the Kendall’s W values were 0.252 and 0.331 (both P < 0.001), indicating a high degree of consistency and reliability in expert opinions. The final competency evaluation scheme for wound, ostomy, and continence specialist nurses covers 5 dimensions, 3 competency levels, and 52 specific items. The survey found that the recognition degree scores for the scheme’s indicators were generally high, and the scheme received positive evaluations for its applicability, executability, and promotability in the professional field. Conclusion: The constructed competency evaluation scheme for wound, ostomy, and continence specialist nurses has good scientific and practical value, and can provide a scientific reference for the capability assessment of nurses in this field.
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