Glioma - initiating cells(GICs) have been proven to play key roles in initiation and progress of malignant ***,a mass of pervious in vivo studies were based on the heterograft model which is implanting GICs derived fr...
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Glioma - initiating cells(GICs) have been proven to play key roles in initiation and progress of malignant ***,a mass of pervious in vivo studies were based on the heterograft model which is implanting GICs derived from primary human glioma specimens into immunodeficient *** this in vivo model could duplicate the microenvironment of malignancy and evaluate the biological features of GICs accurately is ***,we compared the biological features of neurosphere - like tumor cells derived from murine glioma cell line GL261(GL261 - NS) and neurosphere - like tumor cells of primary human glioma specimens(P - NS) in vitro and in *** found that GICs enriched in GL261 - NS,GL261 -NS and P - NS both exhibited similar increased GICs potential and drug resistance in ***,GL261 - NS in syngeneic graft model showed different enhanced tumorigenicity and better duplicated the primary human glioma histologically relative to P - NS in nude mice which were lack of immunocytes and ***,the biological features of P - NS were different among individual largely, although the patients were all diagnosed as *** studies conclude that implantation of GL261 - NS into C57/BL6 mice is a more reliable and stable syngeneic graft model for in vivo study on GICs relative to implantation of P - NS into heterogenous immunodeficient mice.
Oxidative stress plays an important role in the pathological processes of ischemic *** antioxidants have been shown to protect against cerebralischemia injury by inhibiting oxidative stressboth in vitro and in vivo.20...
Oxidative stress plays an important role in the pathological processes of ischemic *** antioxidants have been shown to protect against cerebralischemia injury by inhibiting oxidative stressboth in vitro and in vivo.20 - Hydroxyecdysone(20E),an ecdysteroid hormone exhibits antioxidative ***, we used an in vitro oxidative damagemodel and an in vivo ischemic model of middle cerebral artery occlusion (MCAO) to investigate the neuroprotective effects of 20Eon cerebralischemiainjury and the related *** of cells with HO led to neuronal injury,intracellular ROS/RNS generation, mitochondrial membrane potential dissipation,cellular antioxidant potential descent,the increase in malondialdehyde(MDA),the end product of lipid peroxidation and the elevation of intracellular[Ca]),which were markedly attenuated by *** of the activation of ASK1 - MKK4/7 - JNK stress signalingpathway and caspase - 3 induced by oxidative stresswere involved in the neuroprotection afforded by ***,20E reduced the expression of iNOSproteinby inhibition of NF -κB ***, the protective effect of 20E was confirmed in vivo study.20E significantly decreased infarct volume,the neurological deficit score,restore antioxidant potential and inhibited the increase in MDA and TUNEL positive and caspase - 3 positive cells in the cerebral cortex in ***,these resultssupport that 20E protects against cerebral ischemia injury through multifunctional cytoprotective pathways.
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