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LG1 promotes preligule band formation through directly activating ZmPIN1 genes in maize

Journal of Genetics and Genomics 2025年第3期52卷 356-366页

作者： Zhuojun Zhong Minhao Yao Yingying Cao Dexin Kong Baobao Wang Yanli Wang Rongxin Shen Haiyang Wang Qing Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern AgricultureCollege of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization College of Horticultural Science&TechnologyHeBei Normal University of Science&TechnologyQinhuangdaoHebei 066004China

Increasing plant density is an effective strategy for enhancing crop yield per unit land area.A key architectural trait for crops adapting to high planting density is a smaller leaf angle(LA).Previous studies have demonstrated that LG1,a SQUAMOSA BINDING PROTEIN(SBP)transcription factor,plays a critical role in LA ***,the molecular mechanisms underlying the regulation of LG1 on LA formation remain largely *** this study,we conduct comparative RNA-seq analysis of the preligule band(PLB)region of wild type and lg1 mutant *** Ontology(GO)term enrichment analysis reveals enrichment of phytohormone pathways and transcription factors,including three auxin transporter genes ZmPIN1a,ZmPIN1b,and *** molecular experiments demonstrate that LG1 can directly bind to the promoter region of these auxin transporter genes and activate their *** also show that double and triple mutants of these ZmPINs genes exhibit varying degrees of auricle size reduction and thus decreased *** the contrary,overexpression of ZmPIN1a causes larger auricle and *** together,our findings establish a functional link between LG1 and auxin transport in regulating PLB formation and provide valuable targets for genetic improvement of LA for breeding high-density tolerant maize cultivars.

关键词： Maize(Zea mays L.) LG1 Leaf angle(LA) Preligule band Auxin transport

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Thermal stability of Pd-Au core-shell nanostructures in an in-situ liquid TEM 4

Thermal stability of Pd-Au core-shell nanostructures in an i...

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2024 4th International Conference on Advanced Materials and Chemical Engineering, AMCE 2024

作者： Meng, Dehuan Liu, Shuai Wang, Weiping Wang, Zixin Hu, Xiaoyan Zhang, Xue Information Science Academy of China Electronics Technology Group Corporation Beijing China National Key Laboratory of Integrated Circuits and Microsystems Beijing100042 China China

The investigation of the thermal stability and evolution of Pd-Au core-shell nanostructures across various temperatures proceeded with in situ heating transmission electron microscopy (TEM) along with spectral analysis techniques. In situ heating transmission electron microscopy (TEM) was utilized to examine the morphology and elemental distribution of core-shell nanostructures composed of Pd0.5-Au0.5 and Pd0.9-Au0.1, which were synthesized via the seed growth technique. Compared with the Pd0.5-Au0.5 core-shell nanostructures, the Pd0.9-Au0.1 core-shell nanostructures have better thermal stability. © Published under licence by IOP Publishing Ltd.

关键词： Shells (structures)

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Modeling and prediction of tribological properties of copper/aluminum-graphite self-lubricating composites using machine learning algorithms

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Friction 2024年第6期12卷 1322-1340页

作者： Huifeng NING Faqiang CHEN Yunfeng SU Hongbin LI Hengzhong FAN Junjie SONG Yongsheng ZHANG Litian HU School of Electrical and Mechanical Engineering Lanzhou University of TechnologyLanzhou 730050China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

The tribological properties of self-lubricating composites are influenced by many variables and complex ***-driven methods,including machine learning(ML)algorithms,can yield a better comprehensive understanding of complex problems under the influence of multiple parameters,typically for how tribological performances and material properties *** of friction coefficients and wear rates of copper/aluminum-graphite(Cu/Al-graphite)self-lubricating composites with their inherent material properties(composition,lubricant content,particle size,processing process,and interfacial bonding strength)and the variables related to the testing method(normal load,sliding speed,and sliding distance)were analyzed using traditional approaches,followed by modeling and prediction of tribological properties through five different ML algorithms,namely support vector machine(SVM),K-Nearest neighbor(KNN),random forest(RF),eXtreme gradient boosting(XGBoost),and least-squares boosting(LSBoost),based on the tribology experimental *** demonstrated that ML models could satisfactorily predict friction coefficient and wear rate from the material properties and testing method variables ***,the LSBoost model based on the integrated learning algorithm presented the best prediction performance for friction coefficients and wear rates,with R^(2) of 0.9219 and 0.9243,*** importance analysis also revealed that the content of graphite and the hardness of the matrix have the greatest influence on the friction coefficients,and the normal load,the content of graphite,and the hardness of the matrix influence the wear rates the most.

关键词： self-lubricating composites machine learning(ML) tribological properties prediction

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Selection of protocols for phytoplankton pigment analysis:a comparative study

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Journal of Oceanology and Limnology 2025年第3期43卷 817-830页

作者： Jinxiu WANG Fanzhou KONG Zhuang NIU Rencheng YU CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Qingdao Marine Science and Technology CenterQingdao 266214China University of Chinese Academy of Sciences Beijing 100049China Marine Monitoring and Forecasting Center of Zhejiang Province Hangzhou 310007China

Pigments are widely used as indices for estimation of phytoplankton biomass and composition,and many protocols have been developed to analyze pigments in *** protocols were compared using four solvents(methanol,95%methanol,dimethylformamide,and 90%acetone)and two instruments(fluorometer and high-performance liquid chromatography(HPLC)coupled with diode array detector).analysis of chlorophyll a(Chl a)with fluorometer could lead to over-or underestimation due to the interference from its derivatives in all *** the four extractants,90%acetone had a high recovery for *** contrast,95%methanol was a poor extractant for chlorophylls due to the degradation of Chl a,especially in *** 95%methanol,however,had high extraction efficiencies for most diagnostic ***,the selection of pigment analytical protocols should follow the specific purpose of phytoplankton *** addition to fluorometry,an HPLC method with 90%acetone as extractant shall be a good choice for the analysis of Chl a to estimate phytoplankton biomass,especially for diatom-dominated samples,while an HPLC method with 95%methanol as extractant be more suitable to characterize different taxa in phytoplankton communities.

关键词： pigment fluorometry high-performance liquid chromatography(HPLC) extraction phytoplankton

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GP-Plotter:Flexible Spectral Visualization for Proteomics Data with Emphasis on Glycoproteomics analysis

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Genomics, Proteomics & Bioinformatics 2024年第5期22卷 167-173页

作者： Zheng Fang Mingming Dong Hongqiang Qin Mingliang Ye State Key Laboratory of Medical Proteomics National Chromatographic R.&A.CenterCAS Key Laboratory of Separation Science for Analytical ChemistryDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China School of Bioengineering Dalian University of TechnologyDalian 116024China University of Chinese Academy of Sciences Beijing 100049China

Identification evaluation and result dissemination are essential components in mass spectrometry-based proteomics *** visualization of fragment ions in mass spectrum provides strong evidence for peptide identification and modification ***,we present an easy-to-use tool,named GP-Plotter,for ion annotation of tandem mass spectra and corresponding image *** result files of common searching tools in the community and user-customized files are supported as input of *** display modes and parameter customization can be achieved in GP-Plotter to present annotated spectra of *** image formats,especially vector graphic formats,are available for image generation which is favorable for data ***,GP-Plotter is also well-suited for the visualization and evaluation of glycopeptide spectrum assignments with comprehensive annotation of glycan fragment *** a user-friendly graphical interface,GP-Plotter is expected to be a universal visualization tool for the ***-Plotter has been implemented in the latest version of Glyco-Decipher(v1.0.4)and the standalone GP-Plotter software is also freely available at https://***/DICP-1809.

关键词： Glycoproteomics Glycosylation Proteomics Software Visualization

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Enhancing IoT Service Interface Through AsyncAPI with Extensions 16th

Enhancing IoT Service Interface Through AsyncAPI with Exten...

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16th International Conference on Service science, ICSS 2023

作者： Wang, Hai Wang, Guiling Gao, Jing Hu, Jianhang Li, Junhua Zhang, Haoran School of Information Science and Technology North China University of Technology Beijing100144 China Beijing Key Laboratory on Integration and Analysis of Large-Scale Stream Data Beijing China

ISBN: (纸本)9789819944019

In recent years, various Internet of Things (IoT) applications have penetrated into various areas of human life. In the IoT environment, various IoT service systems perform task collaboration by integrating with a large number of heterogeneous software and hardware devices in an open environment, providing intelligent services to the outside world. However, traditional service applications, when facing IoT with massive data, cannot meet the high real-time requirements of modern applications by relying on a pull-based response method, which also increases system operation pressure. Therefore, an event-driven architecture is adopted to provide IoT services, and service users only need to focus on how to determine the subsequent process based on event content. In this paper, we propose an IoT service interface model based on AsyncAPI, which provides a static expression of service for service subscribers, effectively expresses various operations, and makes IoT service usage more flexible. Secondly, we extend AsyncAPI to define a unified IoT service interface specification, solved the problem of protocol heterogeneity in event transmission. And complete the related code generation mechanism for the transmission of event information between systems. Finally, we apply this interface model to IoT service usage and conduct experimental verification, demonstrating the effectiveness of the IoT service interface model in event-driven communication. © 2023, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

关键词： Internet of things

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Failure analysis of Superheater Tube Crack in Waste Heat Boiler 3

Failure Analysis of Superheater Tube Crack in Waste Heat Boi...

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2024 3rd International Conference on Advances in Modern Physics sciences and Engineering technology, ICPSET 2024

作者： Lu, Xianjing Sun, Xudong Xu, Huiting Ying, Chenkai Yu, Ting Zhejiang Academy of Special Equipment Science Hangzhou310000 China Key Laboratory of Special Equipment Safety Testing Technology of Zhejiang Province Hangzhou310000 China

During the water pressure test of the waste heat boiler of the heavy metal recovery unit, many leaks were found in the superheater tube bundle. These leaks were all concentrated at the simmering bend location of the superheater tube parent material. This prompted an investigation into the causes of cracks in the superheater tubes of the waste heat boiler. The study followed a systematic approach, starting with macroscopic examination of the samples, nondestructive radiography, water quality examination, chemical composition analysis, metallographic analysis, scanning electron microscopy analysis, and energy dispersive spectroscopy (EDS). The results of these analyses revealed that the cracks were expanding from the inner wall to the outer wall, the cracks were dendritic in distribution, and the inner and tip of the cracks were full of grey-white oxides, with typical stress corrosion cracking characteristics. The water quality detected a pH value of 10.7, which was alkaline. This sequence of events ultimately led to the conclusion that the tubes were subjected to alkaline stress corrosion cracking due to the concentration of stresses at the simmering bends, which led to the leakage. © 2024 Institute of Physics Publishing. All rights reserved.

关键词： Stress corrosion cracking

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Lipid-conjugated siRNA hitchhikes endogenous albumin for tumor immunotherapy

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Chinese Chemical Letters 2023年第11期34卷 202-208页

作者： Bo Hu Sudong Kong Yuhua Weng Deyao Zhao Abid Hussain Qingze Jiao Shijing Zhan Ling Qiu Jianguo Lin Minhao Xie Bo Li Yuanyu Huang School of Life Science Advanced Research Institute of Multidisciplinary ScienceSchool of Medical TechnologyKey Laboratory of Molecular Medicine and BiotherapyKey Laboratory of Medical Molecule Science and Pharmaceutics EngineeringBeijing Key Laboratory for Separation and Analysis in Biomedicine and PharmaceuticalsBeijing Institute of TechnologyBeijing 100081China School of Chemical and Material Engineering Jiangnan UniversityWuxi 214122China NHC Key Laboratory of Nuclear Medicine Jiangsu Key Laboratory of Molecular Nuclear MedicineJiangsu Institute of Nuclear MedicineWuxi 214063China Department of Radiation Oncology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000China School of Materials and the Environment Beijing Institute of TechnologyZhuhai 519085China Rigerna Therapeutics Suzhou 215127China

With the development of a small interfering RNA(siRNA)delivery strategy,increasing siRNA therapeutics for tumor treatment appeared in clinical trials and pre-clinical ***,the test results of such therapeutics unveiled that efficient siRNA delivery to tumor tissues is still *** is considered an ideal carrier for delivering hydrophobic agents into tumor tissue because it is highly concentrated and long-circulating in blood and has propensity of tumor ***,we synthesized lipid conjugated siRNAs(LsiRNAs),which showed high affinity to ***,LsiRNAs non-covalently bind to the hydrophobic core of albumin through its octadecyl *** small size of albumin/LsiRNAs allows the complex to penetrate tumor tissue *** test proved that albumin extremely prolonged circulation time and increased tumor retention of associated ***,LsiRNA against programmed death ligand-1(Pdl1)efficiently suppressed tumor growth as well as prolonged survival time of tumor bearing mice by increasing infiltration of CD8^(+)T cells as well as promoted the maturation of dendritic cells both in tumor and ***,LsiRNAs provide a simple but effective way for siRNA tumor delivery that“hitchhikes”on albumin.

关键词： Albumin siRNA Lipid conjugate Tumor treatment Immunotherapy

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Failure analysis of Cracked Pre-Wired Elbows at Flue Gas Inlet 10

Failure Analysis of Cracked Pre-Wired Elbows at Flue Gas Inl...

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10th International Conference on Advances in Machinery, Material science and Engineering Application, MMSE 2024

作者： Yu, Ting Zhong, Fengping Du, Bin Ma, Linlin Xiong, Fahao Zhejiang Academy of Special Equipment Science Hangzhou310000 China Key Laboratory of Special Equipment Safety Testing Technology of Zhejiang Province Hangzhou310000 China

ISBN: (纸本)9781643685465

A smoker inlet pre-wire line, after eight years of use cracking failure, the central performance of the pipeline and elbow body cracks can not be welded, and welding cracks will expand in the elbow. Elbow material is 0Cr18Ni10Ti, working temperature between 0-700 °C, elbow internal circulation medium for flue gas. To study the failure of cracked pre-wired elbows at flue gas inlet, evidence and specimen collection, chemical composition analysis, chemical composition analysis, fracture analysis, metallographic analysis, scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). The experiments confirmed the reason for the cracked pre-wired elbows at flue gas inlet: Smoker inlet pre-wired elbow in the Cr element precipitation at the grain boundary, resulting in Cr-poor area, reducing the corrosion resistance of the material;flue gas impurities in the element S in the grain boundary enrichment, resulting in adsorptive dissolution at the grain boundary;in the case of a significant temperature difference, the use of eight years of pipeline ultimately cracked due to intergranular corrosion. © 2024 The Authors.

关键词： Intergranular corrosion

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Corrosion behavior of liquid level gauge pipe in C5 tank truck 5

Corrosion behavior of liquid level gauge pipe in C5 tank tru...

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5th International Conference on Optoelectronic science and Materials, ICOSM 2023

作者： Li, Qingshan Yang, Lijun Xiong, Mingming Xiao, Zhihua Xia, Xinhua Jin, Muda Xie, Xumeng Zhejiang Academy of Special Equipment Science Hangzhou310009 China Key Laboratory of Special Equipment Safety Testing Technology of Zhejiang Province Hangzhou310009 China

ISBN: (纸本)9781510674547

C5 is a hydrogen sulfide containing media. Corrosion behavior of liquid level gauge pipe was investigated with cross section morphology observation and element analysis in this study. The material of the pipe is 20# steel with massive ferrite and lamellar pearlite. The Vickers hardness of the pipe cross section is 130-150HV. The corrosion product is loose and polyporous with many cracks. The boundary between corrosion layer and metal is generally straight. The corrosion type belongs to uniform corrosion. O and Fe have opposite distribution in the corrosion products layers. Iron-sulfur compounds were observed with SEM-EDS analysis, and S content is up to 2.87%. S mainly distributed at the corrosion products layer, especially at the boundary between the corrosion layer and the matrix metal. © 2024 SPIE.

关键词： Sulfur compounds

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