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The aquaporin MePIP2;7 improves MeMGT9-mediated Mg2+ acquisition in cassava

Journal of Integrative Plant Biology 2023年第10期65卷 2349-2367页

作者： Qiuxiang Ma Yancai Feng Shu Luo Lu Cheng Weijing Tong Xinlu Lu Youzhi Li Peng Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/College of Life Science and Technology Guangxi UniversityNanning 530004China

Aquaporins are important transmembrane water transport proteins which transport water and several neutral molecules. However, how aquaporins are involved in the synergistic transport of Mg2+and water remains poorly understood. Here, we found that the cassava aquaporin Me PIP2;7 was involved in Mg2+transport through interaction with Me MGT9, a lower affinity magnesium transporter protein. Knockdown of Me PIP2;7 in cassava led to magnesium deficiency in basal mature leaves with chlorosis and necrotic spots on their edges and starch over-accumulation. Mg2+content was significantly decreased in leaves and roots of Me PIP2;7-RNA interference(PIP-Ri) plants grown in both field and Mg2+-free hydroponic solution. Xenopus oocyte injection analysis verified that Me PIP2;7 possessed the ability to transport water only and Me MGT9 was responsible for Mg^(2+)*** importantly, Me PIP2;7 improved the transportability of Mg^(2+)via Me MGT9 as verified using the CM66 mutant complementation assay and Xenopus oocytes expressing system. Yeast twohybrid, bimolecular fuorescence complementation,co-localization, and co-immunoprecipitation assays demonstrated the direct protein–protein interaction between Me PIP2;7 and Me MGT9 in vivo. Mg2+fux was significantly elevated in Me PIP2;7-overexpressing lines in hydroponic solution through non-invasive micro-test technique analysis. Under Mg^(2+)-free condition, the retarded growth of PIP-Ri transgenic plants could be recovered with Mg^(2+)supplementation. Taken together, our results demonstrated the synergistic effect of the Me PIP2;7 and Me MGT9 interaction in regulating water and Mg2+absorption and transport in cassava.

关键词： aquaporin cassava magnesium transporter nutrient utilization protein interaction

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Effect of Licorice Extract on Biofilm Formation in Two Pathogenic Bacteria: Aeromonas hydrophila and Yersinia enterocolitica

Journal of the Korean Wood Science and Technology

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Journal of the Korean Wood science and technology 2025年第1期53卷 77-88页

作者： Ham, Youngseok Xu, Delin Kim, Tae-Jong Department of Forest Products and Biotechnology College of Science and Technology Kookmin University Seoul02707 Korea Republic of Department of Ecology Institute of Hydrobiology School of Life Science and Technology Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education Jinan University Guangzhou510632 China

Licorice inhibits the biofilm formation ability and cell growth of Streptococcus mutans, which causes dental caries. In this study, we evaluated the effects of licorice extract on biofilm formation and cell growth of Aeromonas hydrophila and Yersinia enterocolitica to understand strain-specific biofilm formation. Extracts prepared in 50% and 20% ethanol caused the maximum inhibition of A. hydrophila and Y. enterocolitica biofilm formation, respectively. Biofilm formation in A. hydrophila was inhibited in a concentration-dependent manner up to 1.9 g/L 50%-ethanol extract, the maximum tested concentration. An 20%-ethanol extract at 0.47 g/L inhibited Y. enterocolitica biofilm formation by 84%;however, the inhibitory effect did not increase more at higher extract concentrations. The growth of A. hydrophila was unaffected by the licorice extraction, although the growth of Y. enterocolitica decreased up to 0.32 g/L extract. Glycyrrhizin, a previously identified major inhibitory compound against S. mutans, weakly inhibited biofilm formation in both strains, suggesting the presence of additional inhibitory compounds in licorice extract. The inhibitory effects of licorice extract and glycyrrhizin on biofilm formation and cell growth of A. hydrophila and Y. enterocolitica differed from those on S. mutans. In this study, glycyrrhizin was proposed as an active compound inhibiting biofilm formation in A. hydrophila and Y. enterocolitica;however, further studies are warranted to identify compounds that contribute to stronger inhibitory effects and unravel strain-specific biofilm formation mechanisms of A. hydrophila and Y. enterocolitica. © 2025 The Korean Society of Wood science & technology.

关键词： Biofilms

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Covalent organic frameworks-incorporated thin film composite membranes prepared by interfacial polymerization for efficient CO_(2) separation

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Chinese Journal of Chemical Engineering 2022年第3期35卷 152-160页

作者： Haoqing Xu Wenyan Feng Menglong Sheng Ye Yuan Bo Wang Jixiao Wang Zhi Wang Chemical Engineering Research Center School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China Tianjin Key Laboratory of Membrane Science and Desalination Technology State Key Laboratory of Chemical EngineeringCollaborative Innovation Center of Chemical Science and EngineeringTianjin UniversityTianjin 300350China Life and Health Research Institute School of Chemistry and Chemical EngineeringTianjin University of TechnologyTianjin 300384China

Thin film composite(TFC)membranes with nanofillers additives for CO_(2)separation show promising applications in energy and environment-related ***,the poor compatibility between nanofillers and polymers in TFC membranes is the main *** this work,covalent organic frameworks(COFs,TpPa-1)with rich ANHA groups were incorporated into polyamide(PA)segment via in situ interfacial polymerization to prepare defect-free TFC membranes for CO_(2)/N_(2)*** formed covalent bonds between TpPa-1 and PA strengthen the interaction between nanofillers and polymers,thereby enhancing ***,the incorporated COFs disturb the rigid structure of the PA layer,and provide fast CO_(2)transfer *** incorporated COFs also increase the content of effective carriers,which enhances the CO_(2)facilitated ***,in CO_(2)/N_(2)mixed gas separation test,the optimal TFC(TpPa_(0.025)-PIP-TMC/m PSf)membrane exhibits high CO_(2)permeance of 854 GPU and high CO_(2)/N_(2)selectivity of 148 at 0.15 MPa,CO_(2)permeance of 456 GPU(gas permeation unit)and CO_(2)/N_(2)selectivity of 92 at 0.5 *** addition,the Tp Pa_(0.025)-PIP-TMC/m PSf membrane also achieves high permselectivty in CO_(2)/CH_(4)mixed gas separation ***,the optimal TFC membrane showes good stability in the simulated flue gas test,revealing the application potential for CO_(2)capture from flue gas.

关键词： Covalent organic frameworks CO_(2)/N_(2)separation In situ interfacial polymerization Compatibility Covalent bonds

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Impacts of Defoliation on Morphological Characteristics and Non-Structural Carbohydrates of Populus talassica × Populus euphratica Seedlings

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1689-1703页

作者： Mengxu Su Zhanjiang Han Zhen Zhao Xiaofeng Wu Jiaju Wu College of Life Science and Technology Tarim UniversityXinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim BasinAlar843300China Agricultural Science Research Institute of the First Division Xinjiang Production and Construction CorpsAlar843300China

Leaves are important‘source’organs that synthesize organic matter,providing carbon sources for plant ***,we used Populus talassica×Populus euphratica,the dominant species in ecological and timber forests,to simulate carbon limitation through artiﬁcial 25%,50%,and 75%defoliation treatments and explore the effects on root,stem,and leaf morphology,biomass accumulation,and carbon allocation *** the 60th d after treat-ment,under 25%defoliation treatment,the plant height,speciﬁc leaf weight,root surface area and volume,and concentrations of non-structural carbohydrates in stem and root were signiﬁcantly increased by 9.13%,20.00%,16.60%,31.95%,5.12%,and 9.34%,respectively,relative to the *** was no signiﬁcant change in the growth indicators under 50%defoliation treatment,but the concentrations of non-structural carbohydrates in the leaf and stem signiﬁcantly decreased,showing mostly a negative correlation between *** opposite was observed in the *** 75%defoliation treatment,the plant height,ground diameter,leaf number,single leaf area,root,stem,and total biomass were signiﬁcantly reduced by 14.15%,10.24%,14.86%,11.31%,11.56%,21.87%,and 16.82%,respectively,relative to the *** concentrations of non-structural carbohydrates in various organs were signiﬁcantly reduced,particularly in the consumption of the starch concentrations in the stem and *** results indicated that carbon allocation strategies can be adjusted to increase the con-centration of non-structural carbohydrates in root and meet plant growth needs under 25%and 50%***,75%defoliation signiﬁcantly limited the distribution of non-structural carbohydrates to roots and stems,reduced carbon storage,and thus inhibited plant ***-induced carbon limitation altered the carbon allocation pattern of ***×***,and the relationship between carbon reserves in roots and tree growth recovery after defoliation was *** study

关键词： Populus talassica×Populus euphratica defoliation carbon limitation carbon allocation non-structural carbohydrates

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Tracing from Periphery to Cortex:Application of Herpes Simplex Virus to Somatosensory Neural Networks

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Neuroscience Bulletin 2024年第11期40卷 1739-1744页

作者： Yan Chen Qin Chen Yuran Song Huadong Wang Xinyu Hu Kaikai Wang Xuanyuan Wu Fuqiang Xu Lan Bao Xu Zhang Guangdong Institute of Intelligence Science and Technology HengqinZhuhai519031China School of Life Science and Technology ShanghaiTec UniversityShanghai201210China Research Unit of Pain Medicine Chinese Academy of Medical SciencesGuangdong Institute of Intelligence Science and TechnologyHengqinZhuhai519031China NMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Products Brain Cognition and Brain Disease InstituteShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518067China Center for Excellence in Molecular Cell Science Chinese Academy of SciencesShanghai200031China

Dear Editor,Understanding the complex structure of the somatosensory neural network is crucial for deciphering how the brain processes sensory *** advances in viral tracers,such as Herpes simplex virus(HSV)type 1 strain 129(H129),have enabled researchers to effectively map somatosensory neural networks[1-3].However,challenges such as low inoculation efficiency and difficulty in quantitative comparison of labeled neural networks arise due to the polysynaptic nature of H129 and the potential neuronal death induced by its toxicity.

关键词： neural networks Cortex

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Multi-level genomic convergence of secondary aquatic adaptation in marine mammals

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The Innovation 2025年第3期6卷 74-83,73页

作者： Shixia Xu Lei Shan Ran Tian Zhenpeng Yu Di Sun Zhenhua Zhang Inge Seim Ming Zhou Linxia Sun Na Liang Qian Zhang Simin Chai Daiqing Yin Luoying Deme Tianzhen Wu Yongjie Chen Zhikang Xu Yu Zheng Wenhua Ren Guang Yang Jiangsu Key Laboratory for the Biodiversity Conservation and Sustainable Utilization in the Middle and Lower Reaches of Yangtze River Basin College of Life SciencesNanjing Normal UniversityNanjing 210023China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Integrative Biology Laboratory College of Life SciencesNanjing Normal UniversityNanjing 210023China School of Biology and Environmental Science Queensland University of TechnologyBrisbaneQLD 4002Australia

Marine mammals provide a valuable model for studying the molecular basis of convergent evolution during secondary aquatic *** multiomics data and functional experiments,including CRISPR-Cas9 mouse models and luciferase reporter assays,this study explored the molecular mechanisms driving this transition across coding regions,regulatory elements,and genomic *** amino acid substitutions in APPL1P378L and NEIL1E71G were found to promote lipid accumulation and suppress cancer cell proliferation,likely contributing to the evolution of extensive blubber layers and cancer *** evolved conserved non-exonic elements(CNEs)and lineage-specific regulatory variations were shown to influence the activity of nearby genes(e.g.,NKX3-2,SOX9,HAND2),shaping cetacean limb ***,convergent shifts in topologically associating domains(TADs)across cetaceans and pinnipeds were implicated in the regulation of ASXL3 and FAM43B expression,playing a role in the formation of thickened blubber layers and mitigating cancer *** variations within conserved TADs were associated with the expression of neuronal genes,including NUP153 and ID4,potentially driving cognitive and social *** findings provide novel insights into the molecular foundations of the convergent evolution of secondary aquatic adaptations in mammals.

关键词： molecular mechanisms convergent evolution marine mammals secondary aquatic adaptation amino acid substitutions suppress cancer cell multiomics functional experimentsincluding

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The ceRNA Network Indicates Its Immune Function in Peripheral Blood Leukocytes of Half-Smooth Tongue Sole(Cynoglossus semilaevis)After Stimulation with Chitosan Oligosaccharide

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Journal of Ocean University of China 2024年第6期23卷 1615-1629页

作者： WANG Wenwen WEI Shu CHEN Yadong TAN Suxu SHI Kunpeng ZANG Shaoqing WANG Minmin YANG Teng YANG Yingming CUI Zhongkai SHA Zhenxia Institute of Aquatic Biotechnology College of Life SciencesQingdao UniversityQingdao 266071China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology CenterQingdao 266237China

As an efficient immunostimulant,chitosan oligosaccharide(COS)can enhance the immunity of teleosts;however,the underlying molecular mechanisms still require *** endogenous RNAs(ceRNAs)are vital regulators in the immune response,but their roles in half-smooth tongue sole(Cynoglossus semilaevis)remain *** this study,for the first time,we studied whole-transcriptome expression profiles and analyzed ceRNA networks in peripheral blood leukocytes of half-smooth tongue sole treated with COS.A total of 19 circRNAs(DE-circRNAs),18 miRNAs(DE-miRNAs)and 50 previously identified lncRNAs(DElncRNAs)were differentially expressed after COS *** DE-lncRNAs and DE-miRNAs targeted numerous immunity-related genes,and were enriched in important pathways,including MAPK and Toll-like receptor signaling pathways,suggesting the immunoregulatory roles of ***,we constructed circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA regulatory networks using DE-circRNAs,DE-miRNAs,DE-lncRNAs,and ***,a ceRNA network with immunity-related DEmRNAs was constructed,showing that 3 DE-circRNAs,12 DE-lncRNAs,and 29 DEGs exhibited crosstalk through 9 ***,a DE-miRNA in the ceRNA network,miR-144-3p,was targeted by DE-lncRNA tnrc6a,and negatively regulated the genes of inhibitor of nuclear factor kappa B kinases(IKKs)(ikbkg,ikbkb,and ikbip)and ***,ikbkb,and c3ar1 were significantly up-regulated in macrophages stimulated by *** could be inferred that ncRNAs participated in the immune and inflammatory response by acting as ceRNAs after COS stimulation in *** findings indicate that COS could enhance the immunity of teleosts by regulating ncRNAs,and lay the foundation for further practical application of COS in aquaculture.

关键词： ceRNA circRNA miRNA half-smooth tongue sole peripheral blood leukocytes chitosan oligosaccharide

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Ethylene in fruits:beyond ripening control

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园艺研究（英文） 2024年第10期11卷 289-296页

作者： Wei Huang Cong Tan Hongwei Guo State Key Laboratory of Agricultural Genomics Key Laboratory of GenomicsMinistry of AgriculturalBGI ResearchShenzhen 518083China BGI Bioverse Shenzhen 518083China BGI Bioverse Shenzhen 518083China New Cornerstone Science Laboratory Institute of Plant and Food ScienceDepartment of BiologySchool of Life SciencesSouthern University of Science and Technology(SUSTech)ShenzhenGuangdong 518055China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China

Fruits are a rich source of nutrients,minerals,and dietary fibers for both humans and *** the gaseous phytohormone ethylene is well-known for its role in controlling fruit ripening,there is growing evidence that ethylene also plays crucial roles in regulating other developmental processes of fruits,such as sex determination,fruit set,and fruit *** this review,we aim to revisit these findings from various species like cucumber,melon,tomato,rice,maize,and *** studies not only enhance our understanding of ethylene's function in fruits but also highlight the potential for manipulating ethylene to improve ***,we discuss recent studies that show the ethylene precursor ACC(1-AMINOCYCLOPROPANE-1-CARBOXYLATE),and the ethylene signal-ing components EIN2(ETHYLENE INSENSITIVE2)and EIN3(ETHYLENE INSENSITIVE3)have ethylene-independent function in specific *** phenomenon,combined with findings of dosage-dependent ethylene functions in certain conditions,highlights the importance of analyzing mutants with completely blocked ethylene pathways in different species at specific developmental stages and tissue ***,this review offers a timely and essential summary of ethylene's role in sex determination,fruit formation,and fruit growth,which could be beneficial for horticulture crop breeding.

关键词： ethylene beyond control fruits ripening

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Bioprofiling and vaccine development for Zika virus

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science China life sciences 2025年

作者： Huiping Shi Shengyong feng Abid Naeem Baoying Huang Wenjie Tan Lulu Zheng Xingjie Liang Minghui Yang Yuanyu Huang School of Life Science Advanced Research Institute of Multidisciplinary Science Aerospace Center Hospital School of Medical Technology Key Laboratory of Molecular Medicine and Biotherapy Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Beijing Institute of Technology School of Basic Medical Sciences Tsinghua University National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Key Laboratory of Biosafety National Health Commissions National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology of China Advanced Research Institute of Multidisciplinary Science Beijing Institute of Technology (BIT)

Zika virus(ZIKV) has emerged as a global health priority due to its association with severe congenital abnormalities, including microcephaly and neonatal mortality in infants born to infected mothers. In adults, ZIKV infection is epidemiologically linked to Guillain-Barré Syndrome(GBS). These clinical manifestations prompted the World Health Organization(WHO) to declare ZIKV a Public Health Emergency of International Concern(PHEIC). The antibody-dependent enhancement(ADE) phenomenon further complicates vaccine development and therapeutic strategies. While substantial progress has been made in elucidating ZIKV biology through multidisciplinary approaches, no vaccines or therapies have been clinically approved. This review examines ZIKV-associated risks by analyzing transmission dynamics, viral architecture, and cellular entry mechanisms, and proposes evidence-based prevention and management strategies.

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Establishment of a Fah-LSL mouse model to study BEC-to-hepatocyte conversion

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Biophysics Reports 2023年第6期9卷 309-324页

作者： Xingrui Wang Wenjuan Pu Huan Zhu Mingjun Zhang Bin Zhou New Cornerstone Science Laboratory State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of Systems Health Science of Zhejiang Province School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences School of Life Science and Technology Shanghai Tech University

The liver consists predominantly of hepatocytes and biliary epithelial cells(BECs), which serve distinct physiological functions. Although hepatocytes primarily replenish their own population during homeostasis and injury repair, recent findings have suggested that BECs can transdifferentiate into hepatocytes when hepatocyte-mediated liver regeneration is impaired. However, the cellular and molecular mechanisms governing this BEC-to-hepatocyte conversion remain poorly understood largely because of the inefficiency of existing methods for inducing lineage conversion. Therefore, this study introduces a novel mouse model engineered by the Zhou's lab, where hepatocyte senescence is induced by the deletion of the fumarylacetoacetate(Fah) gene. This model facilitates the efficient conversion of BECs to hepatocytes and allows for the simultaneous lineage tracing of BECs; consequently, a transitional liver progenitor cell population can be identified during lineage conversion. This study also outlines the technical procedures for utilizing this model to determine the underlying cellular and molecular mechanisms of BEC-to-hepatocyte conversion and provides new insights into liver regeneration and its underlying molecular mechanism.

关键词： Liver regeneration BEC-to-hepatocyte transdifferentiation Fumarylacetoacetate (Fah) gene Transitional liver progenitor cells (TLPC) Lineage tracing

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