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Chemical characterization of oils and fats from amazonian fruits by1H NMR

Chemical Engineering Transactions 2018年 64卷 235-240页

作者： Santos, Ricardo C. Chagas, Edvan A. de Melo Filho, Antonio A. Takahashi, Jacqueline A. Montero, Ismael F. dos Santos, Gabriel F. Chagas, Pollyana C. de Melo, Cristina G. R. National Postdoctoral Program of CAPES PNPD/CAPES associated to the Postgraduate Program in Agronomy of Universidade Federal de Roraima POSAGRO/UFRR Campus Cauamé BR 174 s/n Km 12 District Monte Cristo Boa Vista-RRCEP 69310-250 Brazil Brazilian Agricultural Research Corporation Embrapa Rodovia 174 Km 8 Industrial District Boa VistaRRCEP 69301970 Brazil Embrapa Boa Vista-RR-Brazil CNPq Research Produtivity Scholarship Brazil Post-Graduate Program in Chemistry PPGQ Center for Research and Graduate Studies in Science and Technology NPPGCT UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Department of Chemistry Institute of Exact Sciences Universidade Federal de Minas Gerais UFMG Av Antonio Carlos no. 6627 Pampulha Belo Horizonte-MGCEP 31270-901 Brazil Post Graduate Program in Biodiversity and Biotechnology Bionorte State Coordination of Roraima UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Post Graduate in Agronomy POSAGRO/UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Post-Graduate in Natural Resources Program PPGRN/UFRR Boa Vista-RRCEP 69304-000 Brazil

ISBN: (纸本)9788895608563

Native plants from the Brazilian Amazon Biome are rich in nutrients, oils and fats and, therefore, represent a very important regional food resource. There is a global interest in the nutritional potential of tropical fruits, the potential of Amazonian fruits as source of essential fatty acids is still under exploited, since their composition is still understudied.1H Magnetic Resonance Nuclear (1H NMR) is a robust spectroscopic tool to profile fatty acids components in oils. The objectives of this work were to use1H NMR to determine the fatty acids profile and the physicochemical properties of crude oils and fats obtained from fruits of Brazilian Amazon: açaí, ata-brava, bacaba, buriti, buritirana, yellow murici, red murici, piaçaba braba, tucumanzinho, pupunha red and yellow varieties. Oils were obtained by Soxhlet extraction using hexane as a solvent. After solvent removal by rotoevaporation, the resulting oils and fats were analyzed by1H NMR spectroscopy. Unsaturated fatty acids oleic (?9), linoleic (?6) and linolenic (?3) acids were quantified in the oils and their physicochemical properties were determined. It was observed that the oils have predominately ?9 fatty acids, followed by ?6 and ?3 representatives. The physicochemical properties confirmed edibility and good quality of oils and fats present in the aforementioned Amazonian fruits. Copyright © 2018, AIDIC Servizi S.r.l.

关键词： Fruits

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Chemical profile, toxicity, anti-acetylcholinesterase and antimicrobial activity of essential oil from hyptis dilatata leaves

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Chemical Engineering Transactions 2018年 64卷 271-276页

作者： Almeida, Sirley P. de Filho, Antonio Alves M. Simplicio, Fernanda Guilhom Martins, Regildo M. G. Takahashi, Jacqueline A. Ferraz, Vany P. de Melo, Ana Cristina G. R. Tadei, Wanderli P. Post graduate in Biodiversity and Biotechnology Program PPG BIONORTE State Coordination of Amazonas ManausAmazonas Brazil Postgraduate Program in Chemistry PPGQ Center for Research and Graduate Studies in Science and Technology NPPGCT Departament of Chemistry and Postgraduate in Biotechnology and Biodiversity Program Rede Bionorte Federal University of Roraima Paricarana Campus Boa Vista-RRCEP 69304-000 Brazil Laboratory of Phytochemistry and Semi synthesis Faculty of Pharmaceutical Sciences Federal University of Amazonas Manaus AmazonasCEP 69077-000 Brazil Federal University of Pernambuco Immuno Pathology Laboratory Keizo-Ami Recife-PE Brazil Department of Chemistry Institute of Exact Sciences Universidade Federal de Minas Gerais CEP 31270-901 Belo HorizonteMinas Gerais Brazil Chromatography Laboratory Institute of Exact Sciences Federal University of Minas Gerais CEP 31270-901 Belo HorizonteMinas Gerais Brazil Post graduate in Natural Resources Program Federal University of Roraima Boa Vista-RR Brazil National Institute for Amazonian Research Manaus AmazonasCEP 69067-375 Brazil

ISBN: (纸本)9788895608563

The aim of this study was to evaluate the influence of seasonality on the chemical composition of the essential oil from Hyptis dilatata leaves, to perform biological activity assays such as antimicrobial, inhibition of acetylcholinesterase enzyme and to evaluate the toxicity of the essential oil using Artemia salina as indicator on the test. Hyptis dilatata leaves were collected in rainy and dry seasons, in the morning, in the afternoon and in the evening. It was extracted by hydrodistillation using the extractor of Clevenger condenser double Spell model. The essential oil analysis resulted in 22 chemical components. The major constituents of the dry and rainy periods were a-pinene (18.7% in the night and 12.9% in the afternoon), 3-carene (26.5% and 19.9% in the night), fenchone (43% and 33.7% in the morning) and ß-caryophyllene 9.1% in the afternoon and 6.1% in the morning. The essential oil in vitro inhibited the acetylcholinesterase enzyme in 99.9% in the afternoon (rainy period) and 96.4% in the morning (dry period). Between the dry and rainy seasons, the lowest LC50 microbial activity in vitro was obtained for leaves collected during rainy season, in the morning period tested against the bacterium Staphylococcus aureus (LC50 78.1 mg mL-1). The cytotoxic activity of the essential oil of H. dilatata on Artemia salina showed LC50 results below 100 µg mL-1 and in the afternoon during the rainy period and at night in the dry period the results are above this value. Therefore, the chemical characterization and biological activities tests of essential oils showed promising results in the search for new active substances and the development of bioproducts of vegetable origin. Copyright © 2018, AIDIC Servizi S.r.l.

关键词： Essential oils

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Isolation and toxicity test of Bacillus thuringiensis from Sekayu region soil, South Sumatra on Spodopteralitura

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IOP Conference Series: Earth and Environmental Science 2018年第1期102卷

作者： S R Afriani Y Pujiastuti C Irsan N Damiri S Nugraha E R Sembiring Study Program of Crop Science Post Graduate Program Sriwijaya University Palembang South Sumatra Indonesia Research Center for Biotechnology Indonesian Institute of Sciences Jakarta Indonesia

This study aimed to obtain bacterial isolates B. thuringiensis potential as a biological control against pests Spodoptera litura. The research was conducted at the Laboratory of Pest and Disease Department, Agricultural Faculty of Sriwijaya University, Campus Inderalaya Ogan Ilir, South Sumatera, from March to June 2017. The study was conducted with survey method and laboratory trial. The results showed that of the 50 soil samples from three villages selected through morphological observation, reaction staining, KOH test, catalase test, producing 13 bacterial isolates. Screening of the 13th toxicity of the isolates suspected B. thuringiensis against S. litura larvae was investigated. Based on the toxicity screening test the following facts were obtained: five isolates ie KJ2M2, KJ3E1, KJ3JB1, KJ3D3 and KJ3D5 were lower toxicity than Dipel, two isolates ie KJ3K4 and KJ3D3 which had the same toxicity to Dipel. Five isolates the KJ3E3, KJ3BW5, KJ3JB5, KJ3D1 and LC2, LC3 known to have effectiveness until the seventh day reached 40%. There was one isolate that is KJ3BW5 which was more effective compared to Dipel as comparison.

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Microstructural and Proteomic Analysis to Investigate the Effectiveness of Papaya Leaf as a Tenderizer of Beef and Goat's Meat

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IOP Conference Series: Earth and Environmental Science 2019年第1期292卷

作者： A I Kartika H S Kusuma S Darmawati D S Tanjung Molecular Biology Laboratory Medical Laboratory Technology D4 Health and Nursing Faculty Universitas Muhammadiyah Semarang Nutrition Laboratory Health and Nursing Faculty Universitas Muhammadiyah Semarang Post Graduate Program in Biotechnology School of Postgraduate Universitas Gadjah Mada St. Teknika Utara Barek Caturtunggal Daerah Istimewa Yogyakarta 55281 Indonesia

Meat quality for the community is determined by the level of tenderizer. The process of meat tenderization could be done by giving papain enzymes derived from papaya fruit or leaves. Meat with the administration of papain enzymes can be analyzed based on protein profiles to see protein and microstructural integrity to see the texture of meat. The research sample was 20 g of beef and goat's meat with the treatment using the enzyme papain derived from papaya leaves. Time variation of enzyme administration 1 h, 2 h, 4 h, and 8 h, as well as variations in the weight of crude papain extract from papaya leaves as much as 10 g, 15 g, and 20 g. Analysis of protein concentration using Bradford and Kjeldhal methods, and protein profiles using the SDS PAGE method, and microstructural analysis using SEM. The concentration of meat protein with the treatment of crude extract of papaya leaves decreased compared to control meat. Based on protein profile analysis showed that meat given the enzyme papain from papaya leaves experienced protein band loss at a size of 225 kDa, 150 kDa, 96 kDa, 86 kDa. Microstructural analysis showed that beef and goat's meat treated with damage to collagen fibers, collagen fibers did not stick with muscle fibers, and the arrangement was irregular, in addition, there was a distance between muscle fibers.

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Biodegradation of BTEX by indigenous microorganisms isolated from UCG project area, South Sumatra

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IOP Conference Series: Earth and Environmental Science 2019年第1期308卷

作者： S Handayani R Safitri W Surono H Astika R Damayanti M Agung Rukiah R&D Centre for Mineral and Coal Technology Ministry of Energy and Mineral Resources Jl. Jend. Sudirman 623 Bandung Indonesia Study Program of Biotechnology Post Graduate School of Padjadjaran University Jl. Dipati Ukur no. 35 Bandung Indonesia. Biology Department Padjadjaran University Jatinangor Indonesia

Recently Indonesia is conducting Underground Coal Gasification (UCG) project in South Sumatra for power *** potential of negative impacts from UCG on groundwater and the broader environment can not be ignored since past similar projects were often confronted with pollution isues of BTEX and PAHs due to condensation of tar-loaded gas. This study focuses on finding indigenous microorganisms capable of BTEX degradation and evaluate their biodegradability. Several microorganisms were successfully isolated and screened. Pseudomonas putida and Bacillus cereus were chosen for this bioremediation study since the bacteria were predominant and highly viable on the screening test. The BTEX degradation has been studied in single component using single and mixed bacterial cultures in the concentration range of 250-500 ppm. The experimental results show that biodegradation of BTEX by P. putida ranged from 61.4-70.2% and by *** ranging 63.9-74.7 % at initial BTEX concentration of 500 ***, consortium of both isolates has the highest percentage of BTEX biodegradation (67.8-79,8%) during 14 days of retention *** findings reveal that indigenous bacteria of P. putida dan B. cereus exhibit the potential to be used for decontamination of BTEX as an anticipated mitigation for potential pollution coming from the UCG project.

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Diversity and longitudinal records: Genetic architecture of disease associations and polygenic risk in the Taiwanese Han population

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Science advances 2025年第23期11卷 eadt0539页

作者： Ting-Yuan Liu Hsing-Fang Lu Yu-Chia Chen Chi-Chou Liao Ying-Ju Lin Jai-Sing Yang Wen-Ling Liao Wei-De Lin Shih-Yin Chen Yu-Chuen Huang Wei-Yong Lin Yu-Huei Liu Kai-Cheng Hsu Shih-Sheng Chang Hong-Da Chen Yu-Pao Chou Jan-Gowth Chang Chung-Hsing Wang Chwen-Tzuei Chang Chung-Ming Huang Kai-Jieh Yeo Tzu-Yuan Wang Chin-Chung Yeh Jiunn-Horng Chen Chi-Ping Huang Hsueh-Chou Lai Rong-Hsing Chen Hui-Ju Lin Po-Yuan Wu Jiu-Yao Wang Chin-Chi Kuo Der-Yang Cho Chang-Hai Tsai Fuu-Jen Tsai Million-person precision medicine initiative Department of Medical Research China Medical University Hospital Taichung 40402 Taiwan. Master Program for Digital Health Innovation China Medical University Taichung 406040 Taiwan. Department of Medical Research China Medical University Hospital Taichung 40402 Taiwan. Genetic Center Department of Medical Research China Medical University Hospital Taichung 40402 Taiwan. School of Post Baccalaureate Chinese Medicine China Medical University Taichung 404333 Taiwan. Graduate Institute of Integrated Medicine College of Chinese Medicine China Medical University Taichung 406040 Taiwan. Center for Personalized Medicine Department of Medical Research China Medical University Hospital Taichung 406040 Taiwan. School of Chinese Medicine China Medical University Taichung 406040 Taiwan. Drug Development Center China Medical University Taichung 406040 Taiwan. Artificial Intelligence Center China Medical University Hospital Taichung 40402 Taiwan. Department of Neurology China Medical University Hospital Taichung 40402 Taiwan. Department of Medicine China Medical University Taichung 406040 Taiwan. Department of Laboratory Medicine China Medical University Hospital Taichung 40402 Taiwan. Department of Bioinformatics and Medical Engineering Asia University Taichung 41300 Taiwan. Department of Medical Laboratory Science and Biotechnology China Medical University Taichung Taiwan. Division of Genetics and Metabolism Children's Hospital of China Medical University Taichung 404447 Taiwan. Intelligent Diabetes Metabolism and Exercise Center Department of Internal Medicine China Medical University Hospital Taichung 40402 Taiwan. Division of Immunology and Rheumatology Department of Internal Medicine China Medical University Hospital Taichung 40402 Taiwan. Division of Endocrine and Metabolism China Medical University Hospital Taichung 40402 Taiwan. Department of Urology China Medical University Hospital Taichung 40402 Taiwan. Cen

We addressed the underrepresentation of non-European populations in genome-wide association studies (GWASs) by building HiGenome, a large-scale genetic resource for the Taiwanese Han population. Using a custom genotyping array, we integrated deidentified electronic medical records (2003 to 2021) with genomic data to enable GWASs, phenome-wide association studies, and polygenic risk score (PRS) analysis. Among 413,000 participants, 323,397 passed ancestry and quality control filtering. GWASs covered 1085 traits, focusing on diseases prevalent in Taiwan such as type 2 diabetes, chronic kidney disease, gout, and alcoholic liver damage. PRSs were calculated for 238 traits, with the strongest associations observed in musculoskeletal disorders. Incorporating PRS into clinical practice supports early risk prediction and personalized prevention. To further expand translational value, we also conducted pharmacogenomic analysis and human leukocyte antigen typing. HiGenome offers a large-scale genetic and clinical dataset from the Taiwanese Han population, supporting population-specific analyses and precision medicine development in East Asia. The hospital-based design enables continuous follow-up and longitudinal data expansion.

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Biodegradation of phenol, anthracene and acenaphthene singly and consortium culture of indigenous microorganism isolates from underground coal gasification area

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IOP Conference Series. Earth and Environmental Science 2019年第1期306卷 012026-012026页

作者： Safitri, R Handayani, S Surono, W Astika, H Damayanti, R Kusmaya, F D Rukiah Balia, R L Biology Department Faculty of Mathematics Universitas Padjadjaran Jalan Raya Bandung Sumedang Km 21 Hegarmanah Jatinangor Kabupaten Sumedang Jawa Barat 45361 Jatinangor Indonesia Program Study Of Biotechnology Post Graduate School Universitas Padjadjaran Jl. Dipati Ukur no. 35 Bandung Indonesia. R&D Centre for Mineral and Coal Technology Jl. Jend. Sudirman 623 Bandung Indonesia Program Study Of Biotechnology Post Graduate School Universitas Padjadjaran Jl. Dipati Ukur no. 35 Bandung Indonesia. R&D Centre for Mineral and Coal Technology Jl. Jend. Sudirman 623 Bandung Indonesia Biology Department Faculty of Mathematics Universitas Padjadjaran Jalan Raya Bandung Sumedang Km 21 Hegarmanah Jatinangor Kabupaten Sumedang Jawa Barat 45361 Jatinangor Indonesia Chemistry Department Faculty of Mathematic and Natural Sciences Universitas Padjadjaran Jalan Raya Bandung Sumedang Km 21 Hegarmanah Jatinangor Kabupaten Sumedang Jawa Barat 45361 Faculty of Animal Husbandry Jl. Raya Bandung Sumedang KM 21 Jatinangor 45363

The gasification of Coal has a potential for long term energy supply in the future by conversion of non-mineable coal seams to syngas for various industrial applications. However, recalcitrant aromatic compounds such as phenol, anthracene, and acenaphthene from UGC are potential to contaminate groundwater, soil and the broader environment. Therefore, the polluted environment required bioremediation. The purpose of this study was to determine the capability of Bacillus velezensis and Stenotropromonas maltophilia isolated from the area of Underground Coal Gasification (UCG) on biodegradation of phenol, anthracene, and acenaphthene in the single and mixed cultures. The degradation of pollutants in the concentration range of 250-500 ppm evaluated by measuring of the ability of bacteria to grow in single component supplemented mineral salt media, screening tests for hydrocarbon-degrading bacteria and biodegradation ability by gravimetric analysis method. The results showed biodegradability of phenol, anthracene, and acenaphthene at concentration 500 ppm after 14 days by S. maltophilia were 60,2%, 80,4%, and 82,5%. B. velezensis showed 79% of biodegradability of phenol, anthracene 79,8%, and acenaphthene 81,1%. The consortium of both isolates has the highest percentage of phenol, anthracene, and acenapthene biodegradation 83,4 %, 83, 53 % and 83,47 % respectively.

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Cinchona cells performance in in vitro culture: quinine alkaloid production with application of different elicitors

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IOP Conference Series: Earth and Environmental Science 2018年第1期185卷

作者： D R Pratiwi Sumaryono P T Sari D Ratnadewi Plant Biology Study Program School of Post-Graduate Program. Bogor Agricultural University Darmaga Campus Bogor 16680 Indonesia Indonesian Research Institute for Biotechnology and Bioindustry Jalan Taman Kencana No.1 Bogor 16128 Indonesia Biology Undergraduate Study Program Bogor Agricultural University Darmaga Campus Bogor 16680 Indonesia Department of Biology Faculty of Mathematics and Natural Sciences Bogor Agricultural University Darmaga Campus Bogor 16680 Indonesia

Quinine is a dominant alkaloid in Cinchona ledgeriana. It is widely used in anti-malaria, anti-cramping, and anti-arrhythmia medication. In addition, it is commercially demanded for its bitter taste in certain soft drinks. The main source of quinine is the bark of Cinchona plant, which consists of 4-7% of the alkaloid. Land use shifting from perennial trees to cash-crops has resulted in a shortage of the plant material for quinine extraction. Plant cell culture has proven its success in producing secondary metabolites, including alkaloids. In principle, plant cells synthesize secondary metabolites when they are exposed to stress conditions. Therefore, in cell culture of C. ledgeriana, two stress-inducing agents i.e. abscisic acid (ABA) and paclobutrazol (PBZ) in combination with mannitol or sorbitol were applied to increase quinine yield. ABA and PBZ depressed the cells growth. Sorbitol caused more stress to cells than mannitol. Its combination with ABA 3 mgL−1 or with PBZ 7 mgL−1, mixed three weeks after culture, produced the highest concentration of quinine followed by mannitol with ABA 3 mgL−1 or PBZ 7 mgL−1 combined at the third week after culture. Cultured cells showed three different shapes: round, oval, and elongated. The elongated shape pre-dominated in the mature cells culture, while round cells pre-dominated in young cells culture. Those mature cells were found to contain alkaloids.

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Correction to: Effect of cement industry flue gas simulation on the physiology and photosynthetic performance of Chlorella sorokiniana

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Journal of Applied Phycology 2017年第2期30卷 873-873页

作者： Eduardo Caffagni Camargo Ana Teresa Lombardi Post-Graduate Program in Ecology and Natural Resources Algal Biotechnology Laboratory Federal University of São Carlos (UFSCar) São Carlos Brazil Botany Department Federal University of São Carlos (UFSCar) São Carlos Brazil

The original version of this article unfortunately contained a mistake. The name of the corresponding author was given as “Eduardo Caffagni de Camargo”. The correct name should be “Eduardo Caffagni Camargo”.

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Abstracts from ISSN Brazil

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JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION 2017年第1期14卷 1-7页

作者： [Anonymous] Laboratory of Nutritional Biochemistry University of Brasilia Brasilia Brazil Applied Physiology & Nutrition Research Group University of São Paulo São Paulo Brazil São Camilo University Centre São Paulo Brazil Catholic University of Brasilia Brasilia Brazil University Center of Brasília Brasilia Brazil GEPAME – São Judas Tadeu University São Paulo Brazil Federal University of Minas Gerais Belo Horizinte Brazil Catolic University of Minas Gerais Belo Horizinte Brazil Federal University of São Paulo São Paulo Brazil Program of Post-graduation in Physical Education Catholic University of Brasilia Brasilia Brazil Graduation in Nutrition Catholic University of Brasilia Brasilia Brazil Centre of Proteomic and Biochemical Analysis Postgraduate Program in Biotechnology and Genomic Sciences Catholic University of Brasilia Brasilia Brazil S-Inova Biotech Graduate Program in Biotechnology Dom Bosco Catholic University Campo Grande Brazil Sports Nutrition Clinic Opeum Brasilia Brazil Natural Drugstore REDEPHARMA Campina Grande Brazil Laboratory of Physiology Nutrition and Health (LAFINS) Faculty of Physical Education and Dance (FEFD) Federal University of Goiás (UFG) Goiania Brazil Exercise and Immunometabolism Research Group Department of Physical Education Universidade Estadual Paulista Presidente Prudente São Paulo Brazil Clinical and Sports Nutrition Research Laboratory (Labince) Nutrition Faculty (FANUT) Federal University of Goias (UFG) Goiânia Brazil Department of Sport School of Physical Education and Sport University of São Paulo São Paulo Brazil Center and Prescription Motor Activity Laboratory Department of Physical Education Universidade Estadual Paulista Presidente Prudente São Paulo Brazil Human Movement Laboratory Universidade São Judas Tadeu São Paulo Brazil Department of Kinesiology Coastal Carolina University Conway USA Department of Physical Education of Universidade Estadual Paulista Bauru Brazil

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