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Optics and the Brain, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences 2023

作者： Lee, Justin Daho Wang, Yihan Nguyen, Amanda Berndt, Andre Molecular Engineering Ph.D. Program University of Washington SeattleWA98195 United States Department of Bioengineering University of Washington SeattleWA98195 United States Institute for Stem Cell and Regenerative Medicine University of Washington SeattleWA98109 United States Department of Pharmacology Center for Neuroscience of Addiction Pain and Emotion University of Washington SeattleWA98195 United States

OxyR is a protein exhibiting exceptional sensitivity and specificity towards H2O2. However, OxyR-based optogenetic sensors do not closely recapitulate the native sensitivity and kinetics. Here, we discuss a novel desi... 详细信息

ISBN: (纸本)9781957171210

关键词： Diffusion Molecules Proteins Sensor performance Sensors Strain

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A reactive oxygen species-responsive hydrogel encapsulated with bone marrow derived stem cells promotes repair and regeneration of spinal cord injury

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Bioactive Materials 2023年第1期19卷 550-568页

作者： Ziming Li Tengfei Zhao Jie Ding Haochen Gu Qiaoxuan Wang Yifan Wang Deteng Zhang Changyou Gao MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China Department of Orthopedics the Second Affiliated Hospital of Zhejiang UniversityHangzhou 310009China School of Medicine Zhejiang UniversityHangzhou 310009China Institute of Neuroregeneration and Neurorehabilitation Qingdao UniversityQingdao 266071China Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cell and Regenerative Medicine Zhejiang UniversityHangzhou 310058China

Spinal cord injury(SCI)is an overwhelming and incurable disabling event accompanied by complicated inflammation-related pathological processes,such as excessive reactive oxygen species(ROS)produced by the infiltrated inflammatory immune cells and released to the extracellular microenvironment,leading to the widespread apoptosis of the neuron cells,glial and *** this study,a thioketal-containing and ROS-scavenging hydrogel was prepared for encapsulation of the bone marrow derived mesenchymal stem cells(BMSCs),which promoted the neurogenesis and axon regeneration by scavenging the overproduced ROS and re-building a regenerative *** hydrogel could effectively encapsulate BMSCs,and played a remarkable neuroprotective role in vivo by reducing the production of endogenous ROS,attenuating ROS-mediated oxidative damage and downregulating the inflammatory cytokines such as interleukin-1 beta(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α),resulting in a reduced cell apoptosis in the spinal cord *** BMSCs-encapsulated ROS-scavenging hydrogel also reduced the scar formation,and improved the neurogenesis of the spinal cord tissue,and thus distinctly enhanced the motor functional recovery of SCI *** work provides a combinational strategy against ROS-mediated oxidative stress,with potential applications not only in SCI,but also in other central nervous system diseases with similar pathological conditions.

关键词： Spinal cord injury(SCI) ROS scavenging BMSCs Axon regeneration Anti-oxidation

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Novel Self-assembled Tellurium Nanoparticles And Their Effects In Myoblastoma cell Line

Journal of Applied Science and Engineering

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Journal of Applied Science and engineering 2025年第11期28卷 2265-2279页

作者： Chithamparam, Pandian Christyraj, Johnson Retnaraj Samuel Selvan Thiagamani, Senthil Muthu Kumar Sriariyanun, Malinee Muthukumaran, Azhaguchamy Department of Biotechnology Kalasalingam Academy of Research and Education Tamil Nadu Krishnankoil India Regeneration and Stem Cell Biology Lab Centre for Molecular and Nanomedical Sciences International Research Centre Sathyabama Institute of Science and Technology Tamil Nadu Chennai600119 India Department of Mechanical Engineering Kalasalingam Academy of Research and Education Anand Nagar Tamil Nadu Krishnankoil626126 India Department of Mechanical Engineering INTI International University Persiaran Perdana BBN Putra Nilai Negeri Sembilan Nilai71800 Malaysia UniversitiTeknologi Malaysia Skudai Johor Johor Bahru81310 Malaysia Biorefinery and Process Automation Engineering Center Department of Chemical and Process Engineering The Sirindhorn International Thai-German Graduate School of Engineering King Mongkut’s University of Technology North Bangkok Bangkok Thailand

Harnessing the distinct features of nanoparticles and nanomaterials for biomedical use has seen remarkable progress in recent years. Tellurium nanoparticles (TeNPs) have attracted attention due to their exceptional properties and potential uses in photoconductivity, nonlinear optics, piezoelectricity, and thermal conductivity applications. This study synthesized TeNPs through a novel heat reduction method using ascorbic acid as a reducing agent, followed by various characterization studies. Functional studies, such as the antibacterial activity, antioxidant activity and cytotoxicity assays, AO/EB staining, and live cell imaging were performed using TeNPs. The various concentrations of TeNPs (5 − 20 µg/ml) result in inhibition zones against *** in the 5-13 mm range and against *** in the 6-12 mm range. The produced TeNPs minimum inhibitory concentration (MIC) demonstrated that these nanoparticles had antibacterial properties against the tested gram-positive and gram-negative bacterial strains. Moreover, TeNPs exhibit strong antioxidant properties and demonstrate high toxicity in C2C12 myoblastoma cells. Notably, at a concentration of 5 µg/ml, TeNPs exhibit potential toxicity. Live cell imaging and cell proliferation assay with high and lower concentrations of TeNPs retards cell growth and proliferation of C2C12 myoblastoma cells compared to control. Based on the results, due to the increased toxicity nature of TeNPs, lower concentrations below 5 µg might be used in scaffolds to enhance cell proliferation for regenerative medicine and tissue engineering applications. © The Author(’s).

关键词： cell proliferation

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Partial Sulfation of Gellan Gum Produces Cytocompatible, Body Temperature-Responsive Hydrogels

SSRN

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SSRN 2022年

作者： Daemi, Hamed Mousavi, Seyed Saeed Keshvari, Hamid Tehran Iran Department of Cell Engineering Stem Cells and Developmental Biology Cell Science Research Center ACECR Royan Institute Tehran Iran

Gellan gum (GG) is a biodegradable polysaccharide and forms thermosensitive hydrogels by a helix-mediated mechanism. Unfortunately, the wide use of GG in tissue engineering has been restricted due to its dramatically higher gelation temperature than normal body temperature. Here, we show that partial sulfation of GG affords a cytocompatible body temperature-responsive hydrogel with an interesting thermoreversibility at 42 °C. The partial sulfation of GG was confirmed by FTIR, EDX and elemental analyses. The sulfated GGs (SGGs) had a higher swelling ratio and degradation in PBS compared to the neat GG. Based on the rheometry analysis, the SGG with a degree of sulfation of 0.27 (H3 sample) showed a gelation temperature near to the physiological temperature. In addition, the drop in mechanical properties of SGGs was compensated by a further calcium-mediated ionic crosslinking, where Young’s modulus of H3 increased from 10.6 ± 1.9 kPa up to 38.4 ± 5.5 kPa. © 2022, The Authors. All rights reserved.

关键词： Hydrogen bonds

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Modulating the Angiogenic and Immunomodulatory Capacities of stem cell-Derived Extracellular Vesicles in Culture Through Mechanical Microenvironment Alteration

SSRN

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SSRN 2024年

作者： Kang, Wu Young Jung, Sunyoung Jeong, Hyundoo Woo, Hyun-Myung Bae, Hojae Cha, Jae Min Department of Biomedical & Robotics Engineering College of Engineering Incheon National University Incheon22012 Korea Republic of 3D Stem Cell Bioengineering Laboratory Research Institute for Engineering and Technology Incheon National University Incheon22012 Korea Republic of Department of Stem Cell and Regenerative Biotechnology KU Convergence Science and Technology Institute Konkuk University Seoul05029 Korea Republic of Institute of Advanced Regenerative Science Konkuk University Seoul05029 Korea Republic of

stem cell-derived extracellular vesicles (EVs) have attracted attention as a promising therapy which could address the limitations of current stem cell treatments. Nevertheless, the inarticulate and inconsistent therapeutic contents of EVs obtained by traditional stem cell culture hinder their clinical applications. This study explores the regulation of the therapeutic efficacy of EVs derived from mesenchymal stem cells (MSCs) through 3D hydrogel culture with controlled mechanical microenvironments. Our results reveal that different physical interactions between MSCs and culture matrices lead to distinct MSC gene expression patterns while preserving the MSCs’ own characteristics as stem cells. In addition, EVs collected from the MSCs showed differentiated angiogenic and immunomodulatory efficacies depending on the mechanical properties of the culture matrices, which was further supported by our comprehensive therapeutic cargo analyses of the cytokines and micro-RNAs in the EVs. As a non-invasive culture approach to stem cells, circumventing genetic modifications or additional biochemical supplementations, our methodology presents a novel avenue for the customized production of EV medicine, potentially advancing clinical applications in regenerative medicine. © 2024, The Authors. All rights reserved.

关键词： stem cells

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In sight on multiple morphological abno rmalities of sperm flagella in male infertility:what is new?

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Asian Journal of Andrology 2020年第3期22卷 236-245页

作者： Wei-Li Wang Chao-Feng Tu Yue-Qiu Tan Institute of Reproductive and Stem Cell Engineering School of Basic Medical ScienceCentral South UniversityChangsha 410078China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410078China National Engineering and Research Center of Human Stem Cell Changsha 410078China

The syndrome of multiple morphological abnormalities of the sperm flagella(MMAF)is a specific kind of asthenoteratozoospermia with a mosaic of flagellar morphological abnormalities(absent,short,bent,coiled,and irregular flagella).MMAF was proposed in 2014 and has attracted increasing attention;however,it has not been clearly *** this review,we elucidate the definition of MMAF from a systematical view,the difference between MMAF and other conditions with asthenoteratozoospermia or asthenozoospermia(such as primary mitochondrial sheath defects and primary ciliary dyskinesia),the knowledge regarding its etiological mechanism and related genetic findings,and the clinical significance of MMAF for intracytoplasmic sperm injection and genetic coun sell *** review provides the basic kno wledge for MMAF and puts forward some suggestions for further investigations.

关键词： asthe no teratozoospermia disease-causing ge nes in tracytoplasmic sperm in jecti on male in fertility multiple morphological abnormalities of the sperm flagella

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Tailoring micro/nano-fibers for biomedical applications

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Bioactive Materials 2023年第1期19卷 328-347页

作者： Bin Kong Rui Liu Jiahui Guo Ling Lu Qing Zhou Yuanjin Zhao Department of Cardio-Thoracic Surgery Institute of Translational MedicineNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical School210008NanjingChina Department of Otolaryngology Head and Neck Surgery Jiangsu Provincial Key Medical DisciplineNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical School210008NanjingChina State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast University210096NanjingChina Institute for Stem Cell and Regeneration Chinese Academy of Science100101BeijingChina

Nano/micro fibers have evoked much attention of scientists and have been researched as cutting edge and hotspot in the area of fiber science in recent years due to the rapid development of various advanced manufacturing technologies,and the appearance of fascinating and special functions and properties,such as the enhanced mechanical strength,high surface area to volume ratio and special functionalities shown in the surface,triggered by the nano or micro-scale *** addition,these outstanding and special characteristics of the nano/micro fibers impart fiber-based materials with wide applications,such as environmental engineering,electronic and biomedical *** review mainly focuses on the recent development in the various nano/micro fibers fabrication strategies and corresponding applications in the biomedical fields,including tissue engineering scaffolds,drug delivery,wound healing,and ***,the challenges for the fabrications and applications and future perspectives are presented.

关键词： Nano/micro fibers Bioprinting Electrospinning Microfluidic spinning Near field electrospinning Biomedical field

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Neuronal and endothelial transglutaminase-2 expression in experimental autoimmune encephalomyelitis and multiple sclerosis

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Neural Regeneration Research 2022年第7期17卷 1471-1472页

作者： Damien D.Pearse Mousumi Ghosh Miami Project to Cure Paralysis University of Miami Miller School of MedicineMiamiFLUSA Department of Neurological Surgery University of Miami Miller School of MedicineMiamiFLUSA Neuroscience Program Interdisciplinary Stem Cell InstituteUniversity of Miami Miller School of MedicineMiamiFLUSA Department of Veterans Affairs Veterans Affairs Medical CenterMiamiFLUSA

Multiple sclerosis(MS)is a neurological condition characterized by the disruption of the blood-brain barrier,immune system activation,and infla mmation that is accompanied by glial reactivity,neuronal cell death,axon demyelination,and *** changes result in functional loss including paralysis,migraine,vision problems,spasticity,and neuropathic pain.

关键词： sclerosis paralysis autoimmune

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Preclinical Assessment of Enhanced Chemodynamic Therapy by an FeMnOx-Based Nanocarrier: Tumor-Microenvironment-Mediated Fenton Reaction and ROS-Induced Chemotherapeutic for Boosted Antitumor Activity

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ACS Applied Materials and Interfaces 2023年第48期15卷 55258-55275页

作者： Dirersa, Worku Batu Kan, Tzu-Chun Getachew, Girum Wibrianto, Aswandi Ochirbat, Sonjid Rasal, Akash Chang, Jungshan Chang, Jia-Yaw Department of Chemical Engineering National Taiwan University of Science and Technology Taiwan Taipei106335 Taiwan Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei11031 Taiwan International Master/Ph.D. Program in Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan International Ph.D. Program for Cell Therapy and Regeneration Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan

In recent studies, iron-containing Fenton nanocatalysts have demonstrated significant promise for clinical use due to their effective antitumor activity and low cytotoxicity. A new approach was reported in this work utilizing cation exchange synthesis to fabricate FeMnOx nanoparticles (NPs) that boost Fenton reactions and responses to the tumor microenvironment (TME) for chemodynamic therapy (CDT) and chemotherapy (CT). Within the TME, the redox metal pair of Fe2+/Mn2+ helps break down endogenous hydrogen peroxide (H2O2) into very harmful hydroxyl radicals (•OH) while simultaneously deactivating glutathione (GSH) to boost CDT performance. To further enhance the therapeutic potential, FeMnOx NPs were encapsulated with thioketal-linked camptothecin (CPT-TK-COOH), a reactive oxygen species (ROS)-responsive prodrug, achieving a high CPT-loading capacity of up to 51.1%. Upon ROS generation through the Fenton reaction, the prodrug TK linkage was disrupted, releasing 80% of the CPT payload within 48 h. Notably, FeMnOx@CPT exhibited excellent dual-modal imaging capabilities, enabling magnetic resonance and fluorescence imaging for image-guided therapy. In vitro studies showed the cytocompatibility of FeMnOx NPs using MDA-Mb-231 and 4T1 cells, but in the presence of H2O2, they induced significant cytotoxicity, resulting in 80% cell death through CDT and CT effects. Upon intravenous administration, FeMnOx@CPT displayed remarkable tumor accumulation, which enhanced tumor suppression in xenografts through improved CDT and CT effects. Moreover, no significant adverse effects were observed in the FeMnOx NP-treated animals. In the current study, the FeMnOx@CPT anticancer platform, with its boosted •OH-producing capability and ROS-cleavable drug release, has been validated utilizing in vitro and animal studies, suggesting its capacity as a viable strategy for clinical trials. © 2023 American Chemical Society.

关键词： Chemotherapy

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Mechano-responsive hydrogel for direct stem cell manufacturing to therapy

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Bioactive Materials 2023年第6期24卷 387-400页

作者： Yufeng Shou Ling Liu Qimin Liu Zhicheng Le Khang Leng Lee Hua Li Xianlei Li Dion Zhanyun Koh Yuwen Wang Tong Ming Liu Zheng Yang Chwee Teck Lim Christine Cheung Andy Tay Department of Biomedical Engineering National University of Singapore117583Singapore Institute for Health Innovation&Technology National University of Singapore117599Singapore School of Civil Engineering and Architecture Wuhan University of Technology430070WuhanChina NUS Tissue Engineering Program National University of Singapore117510Singapore Lee Kong Chian School of Medicine Nanyang Technological University636921Singapore School of Mechanical and Aerospace Engineering Nanyang Technological University639798Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR)138648Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore119288Singapore Mechanobiology Institute National University of Singapore117411Singapore

Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory *** cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in *** overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to *** our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and *** addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK *** our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.

关键词： Mesenchymal stem cell Dynamic mechanical stimulation Magnetic hydrogel stem cell manufacturing cell therapy

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