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Synthesis of Methotrexate-Loaded Dumbbell-Shaped Titanium Dioxide/Gold Nanorods Coated with Mesoporous Silica and Decorated with Upconversion Nanoparticles for Near-Infrared-Driven Trimodal Cancer Treatment

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ACS Applied Materials and Interfaces 2023年第28期15卷 33335-33347页

作者： Dash, Pranjyan Thirumurugan, Senthilkumar Tseng, Ching-Li Lin, Yu-Chien Chen, Sung-Lung Dhawan, Udesh Chung, Ren-Jei Taipei10608 Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei110 Taiwan International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei110 Taiwan Research Center of Biomedical Device College of Biomedical Engineering Taipei Medical University Taipei110 Taiwan International Ph.D. Program in Cell Therapy and Regenerative Medicine College of Medicine Taipei Medical University Taipei110 Taiwan Centre for the Cellular Microenvironment Division of Biomedical Engineering James Watt School of Engineering Mazumdar-Shaw Advanced Research Centre University of Glasgow GlasgowG116EW United Kingdom Taipei10608 Taiwan

This study prepared dumbbell-shaped titanium dioxide (TiO2)/gold nanorods (AuNRs) coated with mesoporous silica shells (mS) (AuNRs-TiO2@mS). Methotrexate (MTX) was further loaded into the AuNRs-TiO2@mS, and then upconversion nanoparticles (UCNPs) were decorated to form AuNRs-TiO2@mS-MTX: UCNP nanocomposites. TiO2 is used as an intense photosensitizer (PS) to produce cytotoxic reactive oxygen species (ROS), leading to photodynamic therapy (PDT). Concurrently, AuNRs exhibited intense photothermal therapy (PTT) effects and photothermal conversion efficiency. In vitro results suggested that these nanocomposites can kill oral cancer cells (HSC-3) without toxicity through irradiation of NIR laser, owing to the synergistic effect. The in vivo studies indicated that these nanocomposites exhibited excellent antitumor effects through synergistic PDT/PTT/chemotherapy under a near-infrared (NIR) 808 nm laser irradiation. Thus, these AuNRs-TiO2@mS: UCNP nanocomposites have great potential to undergo deep tissue penetration with enhanced synergistic effects through NIR-triggered light for cancer treatment. © 2023 American Chemical Society

关键词： Titanium dioxide

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Self-organization principles in stem-cell-derived synthetic embryo models

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The Innovation 2023年第1期4卷 20-21页

作者： Min Bao California Institute of Technology Division of Biology and Biological Engineering1200 E.California BoulevardPasadenaCA 91125USA Mammalian Embryo and Stem Cell Group Department of PhysiologyDevelopmentand NeuroscienceUniversity of CambridgeCambridge CB23DYUK Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou325001ZhejiangChina

As embryos,we were extremely lucky to survive implantation,when we transitioned from a simple ball of undifferentiated cells to a complex structure of distinct *** development,fertilization generates a totipotent zygote that ultimately gives rise to themyriad cell types of the whole *** embryos,the first cell fate decision leads to trophectoderm(TE)progenitors on the outside of the early embryo,surrounding the inner cell ***(EPI)and primitive endoderm(PE)cells then arise from the inner cell mass and are initially intermingled(embryonic day 3.5).Thus,themammalian pre-implantation embryo comprises three lineage compartments:TE,destined to become the placenta;PE,destined to become the yolk sac;and EPI,pluripotent cells that will generate the entire body.

关键词： implantation initially outside

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IRE1α regulates the PTHrP-IHH feedback loop to orchestrate chondrocyte hypertrophy and cartilage mineralization

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Genes & Diseases 2024年第1期11卷 464-478页

作者： Mengtian Fan Nana Geng Xingyue Li Danyang Yin Yuyou Yang Rong Jiang Cheng Chen Naibo Feng Li Liang Xiaoli Li Fengtao Luo Huabing Qi Qiaoyan Tan Yangli Xie Fengjin Guo Laboratory of Developmental Biology Department of Cell Biology and GeneticsSchool of Basic Medical ScienceChongqing Medical UniversityChongqing 400016China Laboratory of Stem Cells and Tissue Engineering School of Basic Medical ScienceChongqing Medical UniversityChongqing 400016China Department of Orthopedics The 1st Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Wound Repair and Rehabilitation Medicine State Key Laboratory of TraumaBurns and Combined InjuryTrauma CenterResearch Institute of SurgeryDaping HospitalArmy Medical UniversityChongqing 400042China

Cartilage development is controlled by the highly synergistic proliferation and differentiation of growth plate chondrocytes,in which the Indian hedgehog(IHH)and parathyroid hormone-related protein-parathyroid hormone-1 receptor(PTHrP-PTH1R)feedback loop is *** inositol-requiring enzyme 1a/X-box-binding protein-1 spliced(IRE1α/XBP1s)branch of the unfolded protein response(UPR)is essential for normal cartilage ***,the precise role of ER stress effector IRE1α,encoded by endoplasmic reticulum to nucleus signaling 1(ERN1),in skeletal development remains ***,we reported that loss of IRE1α accelerates chondrocyte hypertrophy and promotes endochondral bone ***1 acts as a negative regulator of chondrocyte proliferation and differentiation in postnatal growth *** deficiency interrupted PTHrP/PTH1R and IHH homeostasis leading to impaired chondrocyte hypertrophy and ***1s,produced by p-IRE1α-mediated splicing,binds and up-regulates PTH1R and IHH,which coordinate cartilage ***,ER stress cannot be activated normally in ERN1-deficient *** conclusion,ERN1 deficiency accelerates chondrocyte hypertrophy and cartilage mineralization by impairing the homeostasis of the IHH and PTHrP/PTH1R feedback loop and ER ***1 may have a potential role as a new target for cartilage growth and maturation.

关键词： Cartilage development ER stress ERN1 IHH PTHrP/PTH1R

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Human amniotic epithelial stem cell is a cell therapy candidate for preventing acute graft-versus-host disease

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中国药理学报（英文版） 2024年第11期45卷 2339-2353页

作者： Peng-jie Yang Xiang-yu Zhao Yao-hui Kou Jia Liu Xiang-yi Ren Yuan-yuan Zhang Zhi-dong Wang Zhen Ge Wei-xin Yuan Chen Qiu Bing Tan Qin Liu Yan-na Shi Yuan-qing Jiang Cong Qiu Li-he Guo Jin-ying Li Xiao-jun Huang Lu-yang Yu MOE Laboratory of Biosystems Homeostasis & Protection of College of Life Sciences Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province of Sir Run Run Shaw HospitalZhejiang UniversityHangzhou 310058China Peking University Institute of Hematology Beijing Key Laboratory of Hematopoietic Stem Cell TransplantationPeking University People's HospitalPeking UniversityBeijing 100044China MOE Laboratory of Biosystems Homeostasis & Protection of College of Life Sciences Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province of Sir Run Run Shaw HospitalZhejiang UniversityHangzhou 310058China College of Life Sciences-iCell Biotechnology Regenerative Biomedicine Laboratory Joint Research Centre for Engineering BiologyZhejiang University-University of Edinburgh InstituteZhejiang UniversityHangzhou 314400China School of Pharmaceutical Sciences Hangzhou Medical CollegeHangzhou 310013China College of Life Sciences-iCell Biotechnology Regenerative Biomedicine Laboratory Joint Research Centre for Engineering BiologyZhejiang University-University of Edinburgh InstituteZhejiang UniversityHangzhou 314400China Shanghai iCELL Biotechnology Co.Ltd Shanghai 200335China Shanghai iCELL Biotechnology Co.Ltd Shanghai 200335China College of Life Sciences-iCell Biotechnology Regenerative Biomedicine Laboratory Joint Research Centre for Engineering BiologyZhejiang University-University of Edinburgh InstituteZhejiang UniversityHangzhou 314400China Shanghai iCELL Biotechnology Co.Ltd Shanghai 200335China institute of Biochemistry and Cell Biology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Peking University Institute of Hematology Beijing Key Laboratory of Hematopoietic Stem Cell TransplantationPeking University People's HospitalPeking UniversityBeijing 100044China Peking-Tsinghua Center for Life Sciences Beijing 100084China

Graft-versus-host disease(GVHD),an immunological disorder that arises from donor T cell activation through recognition of host alloantigens,is the major limitation in the application of allogeneic hematopoietic stem cell transplantation(allo-HSCT).Traditional immunosuppressive agents can relieve GVHD,but they induce serious side *** is highly required to explore alternative therapeutic *** amniotic epithelial stem cells(hAESCs)were recently considered as an ideal source for cell therapy with special immune regulatory *** this study,we evaluated the therapeutic role of hAESCs in the treatment of GVHD,based on our previous developed cGMP-grade hAESCs *** mouse model of acute GVHD(aGVHD)was established by injection of huPBMCs via the tail *** prevention or treatment of aGVHD,hAESCs were injected to the mice on day-1 or on day 7 post-PBMC infusion,*** showed that hAESCs infusion significantly alleviated the disease phenotype,increased the survival rate of aGVHD mice,and ameliorated pathological injuries in aGVHD target *** demonstrated that hAESCs directly induced CD4+T cell polarization,in which Th1 and Th17 subsets were downregulated,and Treg subset was ***,the levels of a series of pro-inflammatory cytokines were reduced while the levels of the anti-inflammatory cytokines were upregulated in the presence of *** found that hAESCs regulated CD4+subset polarization in a paracrine mode,in which TGFβ and PGE2 were selectively secreted to mediate Treg elevation and Th1/Th17 inhibition,*** addition,transplanted hAESCs preserved the graft-versus-leukemia(GVL)effect by inhibiting leukemia cell *** intriguingly,hAESCs infusion in HSCT patients displayed potential anti-GVHD effect with no safety concerns and confirmed the immunoregulatory mechanisms in the preclinical *** conclude that hAESCs infusion is a promising therapeutic strategy for post-HSCT GVHD without c

关键词： graft-versus-host disease hematopoietic stem cell transplantation human amniotic epithelial stem cells immunomodulation

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Long-term,multidomain analyses to identify the breed and allelic effects in MSTN-edited pigs to overcome lameness and sustainably improve nutritional meat production

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Science China(Life Sciences) 2022年第2期65卷 362-375页

作者： Ziyao Fan Zhiguo Liu Kui Xu Tianwen Wu Jinxue Ruan Xinmin Zheng Shideng Bao Yulian Mu Tad Sonstegard Kui Li State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding Hubei Institute of Animal Science and Veterinary MedicineHubei Academy of Agricultural SciencesWuhan 430064China Department of Stem Cell Biology and Regenerative Medicine Lerner Research InstituteCleveland ClinicCleveland OH 44195USA Acceligen Inc. Eagan MN 55121USA

Beef and mutton production has been aided by breeding to integrate allelic diversity for myostatin(MSTN),but a lack of diversity in the MSTN germplasm has limited similar advances in pig ***,insurmountable challenges with congenital lameness and a dearth of data about the impacts of feed conversion,reproduction,and meat quality in MSTN-edited pigs have also currently blocked ***,in a largest-to-date evaluation of multiple MSTN-edited pig populations,we demonstrated a practical alternative edit-site-based solution that overcomes the major production obstacle of hindlimb *** also provide long-term and multidomain datasets for multiple breeds that illustrate how MSTN-editing can sustainably increase the yields of breed-specific lean meat and the levels of desirable lipids without deleteriously affecting feed-conversion rates or litter *** from establishing a new benchmark for the data scale and quality of genome-edited animal production,our study specifically illustrates how gene-editing site selection profoundly impacts the phenotypic outcomes in diverse genetic back-grounds.

关键词： MSTN-edited pigs multidomain evaluation genetic background editing strategy

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Extraction, Characterization, and Biocompatibility Evaluation of Type I Collagen from Chicken Feet Skin

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Biomedical Materials and Devices 2025年 1-15页

作者： Zourazema, Sevda Zendeh, Zahra Aksun, Elif Tugce Gorgun, Busra Guzel, Fatma Dogan Cecen, Berivan Kozaci, Leyla Didem Department of Translational Medicine Institute of Health Sciences Ankara Yildirim Beyazit University Ankara 06800 Türkiye Department of Food Processing-Food Technology Vocational School of Health Services Ankara Yildirim Beyazit University Ankara 06010 Türkiye Faculty of Engineering and Natural Sciences Department of Biomedical Engineering Ankara Yildirim Beyazit University Ankara Türkiye Department of Biomedical Engineering Newark College of Engineering New Jersey Institute of Technology Newark 07102 NJ United States Stem Cell and Tissue Engineering Application and Research Center Istinye University Istanbul 34010 Türkiye Department of Medical Biochemistry Faculty of Medicine Ankara Yildirim Beyazit University Ankara 06800 Türkiye

Abstract: The study focused on extracting type I collagen, a protein derived from chicken feet skin, for applications in the biomedical and cosmetic industries. The research thoroughly explored the extraction methodology and characterization techniques and assessed the biocompatibility of the collagen. Acid extraction yielded substantial amounts of type I collagen with an efficiency of 11.35%. The characterization process involved various analyses, including FTIR, SDS-PAGE, western blot, and SEM imaging, to evaluate purity, structural integrity, and morphology. In vitro testing confirmed the biocompatibility of the extracted collagen, indicating its potential as a natural polymer for medical studies. The collagen extracted from chicken feet skin demonstrated significant bioactivity, positioning it as a promising polymer for tissue engineering, wound healing, and cosmetic applications. This study underlines the importance of developing biocompatible biomaterials and provides valuable insights into a sustainable source of collagen from animal by-products. © This is a U.S. Government work and not under copyright protection in the US;foreign copyright protection may apply 2024.

关键词： Biocompatibility Chicken foot skin Type I collagen extraction

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Nidogen:A matrix protein with potential roles in musculoskeletal tissue regeneration

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Genes & Diseases 2022年第3期9卷 598-609页

作者： Sheng Zhou Song Chen Yixuan Amy Pei Ming Pei Stem Cell and Tissue Engineering Laboratory Department of OrthopaedicsWest Virginia UniversityMorgantownWV 26506USA Department of Orthopaedics The GeneraLHospital of Western Theater CommandChengduSichuan 610083PR China WVU Cancer Institute Robert C.Byrd Health Sciences CenterWest Virginia UniversityMorgantownWV 26506USA

Basement membrane proteins are known to guide cell structures,differentiation,and tissue *** there is a wealth of knowledge on the functions of laminins,per-lecan,and type IV collagen in maintaining tissue homeostasis,not much is known about *** a key molecule in the basement membrane,nidogen contributes to the formation of a delicate microenvironment that proves necessary for stem cell lineage-specific *** this review,the expression of nidogen is delineated at both cellular and tissue levels from embryonic to adult stages of development;the effect of nidogens is also summarized in the context of musculoskeletal development and regeneration,including but not limited to adipogenesis,angiogenesis,chondrogenesis,myogenesis,and ***,potential mechanisms underlying the role of nidogens in stem cell-based tissue regeneration are also *** concise review is expected to facilitate our existing understanding and utilization of nidogen in tissue engineering and regeneration.

关键词： Adipose Basement membrane Cartilage Differentiation Nerve Nidogens Vessel

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Aluminum exposure-exacerbated experimental colitis in mice were antagonized by Pueraria lobata extract

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Food Science and Human Wellness 2024年

作者： Beibei Peng Hui Zhao Fan Yang Jie Pan Liang Bai Zhibo Han Wenqiang Guan Jianfu Liu Yongqing Tao Zheng Wang Tianjin Key Laboratory of Food and Biotechnology， State Experimental and Training Centre of Food and Drug， School of Biotechnology and Food Science， Tianjin University of Commerce Department of Pi-Wei Disease， Xuanwu Traditional Chinese Medical Hospital State Key Lab of Experimental Hematology， Institute of Hematology and Blood Diseases Hospital， Chinese Academy of Medical Sciences Jiangxi Engineering Research Center for Stem Cell

Krill oil （KO） exhibits various biological activities， such as anti-inflammatory and antitumor effects. However， the inhibitory effects of benign prostatic hyperplasia （BPH） in vitro and in vivo have not yet been studied. This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method. KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G0/G1phase arrest through the modulation of positive and negative regulators in both prostate cell types. KO treatment stimulated phosphorylation of JNK and p38 signaling. In addition， KO changed the expression of BPH-related markers （5α-reductase， androgen receptor， FGF， Bcl-2， and Bax） and the activity of the proliferation-mediated NF-κB binding motif. KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38 （si-p38） and JNK （si-JNK）. Furthermore， the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model. KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues. Finally， KO-mediated inhibition of prostatic growth was validated by histological analysis. These results suggest that KO has an inhibitory effect on BPH in prostate cells in vitro and in vivo. Thus， KO might be a potential prophylactic or therapeutic agent for patients with BPH.

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Superparamagnetic iron oxide nanoparticle regulates microbiota-gut-inner ear axis for hearing protection

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National Science Review 2024年第6期11卷 273-284页

作者： Zhanhang Guo Yunhao Wu Bo Chen Mengdie Kong Peng Xie Yan Li Dongfang Liu Renjie Chai Ning Gu Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Science and Medical EngineeringSoutheast University Medical Science and Technology Innovation Center Shandong First Medical University &Shandong Academy of Medical Sciences State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and Technology School of Medicine Advanced Institute for Life and Health Jiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSoutheast University Institute of Materials Science and Devices School of Materials Science and Engineering Suzhou University of Science and Technology Nurturing Center of Jiangsu Province for State Laboratory of AI Imaging &Interventional Radiology & Vascular Surgery Department of Radiology Medical School Zhongda Hospital Southeast University Co-Innovation Center of Neuroregeneration Nantong University School of Medical Technology Institute of Engineering MedicineBeijing Institute of Technology Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of China Institute for Stem Cell and Regeneration Chinese Academy of Sciences Southeast university Shenzhen research institute Cardiovascular Disease Research Center Nanjing Drum Tower Hospital Affiliated Hospital of Medical School Medical School Nanjing University

Noise-induced hearing loss(NIHL) is a highly prevalent form of sensorineural hearing damage that has significant negative effects on individuals of all ages and there are no effective drugs approved by the US Food and Drug Administration. In this study, we unveil the potential of superparamagnetic iron oxide nanoparticle assembly(SPIOCA) to reshape the dysbiosis of gut microbiota for treating NIHL. This modulation inhibits intestinal inflammation and oxidative stress responses, protecting the integrity of the intestinal barrier. Consequently, it reduces the transportation of pathogens and inflammatory factors from the bloodstream to the cochlea. Additionally, gut microbiota-modulated SPIOCA-induced metabolic reprogramming in the gut–inner ear axis mainly depends on the regulation of the sphingolipid metabolic pathway, which further contributes to the restoration of hearing function. Our study confirms the role of the microbiota–gut–inner ear axis in NIHL and provides a novel alternative for the treatment of NIHL and other microbiota dysbiosis-related diseases.

关键词： superparamagnetic iron oxide nanoparticle hearing loss gut microbiota inflammation sphingolipid metabolism microbiota–gut–inner ear axis

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Influence of sperm morphology on pregnancy outcome and offspring in in vitro fertilization and intracytoplasmic sperm injection:a matched case-control study

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Asian Journal of Andrology 2021年第4期23卷 421-428页

作者： Wen-Jun Zhou Chuan Huang Su-Hua Jiang Xi-Ren Ji Fei Gong Li-Qing Fan Wen-Bing Zhu Institute of Reproductive and Stem Cell Engineering Basic Medicine CollegeCentral South UniversityChangsha 410008China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410008China Clinical Research Center for Reproduction and Genetics in Hunan Province Changsha 410008China

Sperm morphology was once believed as one of the most predictive indicators of pregnancy outcome in assisted reproductive technology(ART).However,the impact of teratozoospermia on in vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI)outcomes and its offspring remains *** order to evaluate the influence of teratozoospermia on pregnancy outcome and newborn status after IVF and ICSI,a retrospective study was *** was a matched case-control study that included 2202 IVF cycles and 2574 ICSI cycles and was conducted at the Reproductive and Genetic Hospital of CITIC-Xiangya in Changsha,China,from June 2013 to June *** were divided into two groups based on sperm morphology:teratozoospermia and normal sperm *** pregnancy outcome and newborn outcome were *** results indicated that couples with teratozoospermia had a significantly lower optimal embryo rate compared to those with normal sperm morphology in IVF(P=0.007),while there were no statistically significant differences between the two groups in terms of the fertilization rate,cleavage rate,implantation rate,and pregnancy rate(all P>0.05).Additionally,teratozoospermia was associated with lower infant birth weight in multiple births after *** regard to ICSI,there was no significant difference in both pregnancy outcome and newborn outcome between the teratozoospermia and normal groups(both P>0.05).Furthermore,no increase in the risk of birth defects occurred in the teratozoospermia group after IVF/***,we believe that teratozoospermia has limited predictive value for pregnancy outcomes in IVF/ICSI,and has little impact on the resulting offspring if multiple pregnancy is avoided.

关键词： in vitro fertilization intracytoplasmic sperm injection newborn outcome pregnancy outcome teratozoospermia

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